Development of a new virus-induced gene silencing vector using Apple latent spherical virus
Project/Area Number |
20380025
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Plant pathology
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Research Institution | Iwate University |
Principal Investigator |
YOSHIKAWA Nobuyuki Iwate University, 農学部, 教授 (40191556)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2009: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2008: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
|
Keywords | ウイルスベクター / リンゴ小球形潜在ウイルス(ALSV) / ウイルス誘導ジーンサイレンシング(VIGS) / 遺伝子機能解析 / ダイズ / バラ科果樹 / ALSV / ウイルス誘導ジーンサイレンシング / RNAサーイレンシング / VIGS / タバコ / シロイヌナズナ / 抵抗性遺伝子 / RNAサイレンシング / ウリ科植物 / マメ科植物 / ナス科植物 / 遺伝子 |
Research Abstract |
A virus-induced gene silencing (VIGS) system using Apple latent spherical virus (ALSV) vectors was established in legume, cucurbit, Solanaceae species, and Rosaceae fruit trees. ALSV vectors could be successfully used to silence disease resistant N gene in tobacco and RCY1 gene in Arabidopsis thaliana. Infection with an ALSV vector having a fragment of soybean isoflavone synthase 2 gene also led to a reduction of the levels of an isoflavone content in the cotyledons of infected seeds.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Seed and pollen transmission of Apple latent spherical virus in apple2011
Author(s)
Nakamura, K., Yamagishi, N., Isogai, M., Komori, S., Ito, T., Yoshikawa, N.
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Journal Title
Journal of General Plant Pathology
Volume: 77
Pages: 48-53
NAID
Related Report
Peer Reviewed
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[Journal Article] Promotion of flowering and reduction of a generation time in apple seedlings by ectipical expression of the Arabidopsis thaliana FT gene using the Apple latent spherical virus vector2011
Author(s)
Yamagishi, N.sasaki, S., Yamagata, K, Komori, S., Nagase, M., Wada, M., Yamamoto, T., Yoshikawa, N.
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Journal Title
Plant Molecular Biology
Volume: 75
Pages: 193-204
Related Report
Peer Reviewed
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[Journal Article] Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes2009
Author(s)
Igarashi A., Yamagata K., Sugai T.,Takahashi Y., Sugawara E., Tamura A., Uaegasjo H., Yamagishi, N., Takahashi T., Isogai M., Takahashi H., Yoshikawa N.
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Journal Title
Virology 386
Pages: 407-416
Related Report
Peer Reviewed
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[Journal Article] Apple latent spherical virus vectors for reliable and effective virus-ind uced gene silencing among a broad range of plants including tobacco, tomato, Arabidopsis thaliana, cucurbits, and legumes2009
Author(s)
Igarashi, A., Yamagata, K., Sugai, T., Takahashi, Y., Sugawara, E., T amura, A., Yaegashi, H., Yamagishi, N., Takahashi, T., Isogai, M.,Takahashi, H., and Yoshikawa, N.
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Journal Title
Virology 386
Pages: 407-416
Related Report
Peer Reviewed
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