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Functional analysis of glycolysis enzyme GAPDH in HR cell death

Research Project

Project/Area Number 20380030
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant pathology
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

NISHIZAWA Yoko  National Institute of Agrobiological Sciences, 植物・微生物間相互作用研究ユニット, 上級研究員 (40355756)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2009: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2008: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywords植物免疫 / 過敏感細胞死 / MAMPs受容体 / イネ / ユビキチンリガーゼ / 活性酸素 / 活性窒素 / いもち病
Research Abstract

Various defense reactions such as hypersensitive cell death are induced in plants on recognition of microbe-associated molecular patterns (MAMPs). In this study, we showed that chitin oligosaccharides, which are representative fungal MAMPs, elicit the production of both reactive oxygen and nitrogen species, and the production of these radicals is required for the induction of hypersensitive cell death in rice cultured cells. In addition, we performed functional analysis of rice EL5 gene, whose expression is induced by chitin, confirmation of interaction between EL5 and GAPDH in rice cells, and characterization of GAPDH during cell death. Our data indicate that GAPDH, a glycolysis enzyme, is also involved in the induction of cell death in plants.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (24 results)

All 2011 2010 2009 2008

All Journal Article (7 results) (of which Peer Reviewed: 3 results) Presentation (14 results) Book (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Enhancement of MAMP signaling by chimeric receptors improves disease resistance in plants.2011

    • Author(s)
      K.Kishimoto, Y.Kouzai, H.Kaku, N.Shibuya, E.Minami, Y.Nishizawa
    • Journal Title

      Plant Signaling & Behavior 6(3)

      Pages: 449-451

    • Related Report
      2010 Final Research Report
  • [Journal Article] Enhancement of MAMP signaling by chimeric receptors improves disease resistance in plants2011

    • Author(s)
      Kishimoto, et al.
    • Journal Title

      Plant Signaling & Behavior

      Volume: 6 Pages: 449-451

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Localization of probe-accessible chitin and characterization of genes encoding chitin-binding domains during rice-Magnaporthe oryzae interactions2011

    • Author(s)
      Mochizuki, et al.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 77 Pages: 163-173

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Perception of the chitin oligosaccharides contributes to disease resistance to blast fungus Magnaporthe oryzae in rice.2010

    • Author(s)
      K.Kishimoto, Y.Kouzai, H.Kaku, N.Shibuya, E.Minami, Y.Nishizawa
    • Journal Title

      The Plant Journal 64

      Pages: 343-354

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Perception of the chitin oligosaccharides contributes to disease resistance to blast fungus Magnaporthe oryzae in rice2010

    • Author(s)
      Kishimoto, et al.
    • Journal Title

      The Plant Journal

      Volume: 64 Pages: 343-354

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2010

    • Author(s)
      西澤洋子
    • Journal Title

      微生物と植物の相互作用:病害の生物防除(ソフトサイエンス社)

      Pages: 117-122

    • Related Report
      2009 Annual Research Report
  • [Journal Article] EL5 is involved in root development as an anti-cell death ubiquitin ligase.2008

    • Author(s)
      Y.Nishizawa, S.Katoh, H.Koiwai, E.Katoh
    • Journal Title

      Plant Signaling & Behavior 3(2)

      Pages: 148-150

    • Related Report
      2010 Final Research Report
  • [Presentation] キチンオリゴ糖応答性の改変によるイネの菌類病抵抗性増強の試み2011

    • Author(s)
      香西雄介・岸本久太郎・賀来華江・渋谷直人・南栄一・西澤洋子
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      講演要旨集のみ
    • Year and Date
      2011-03-11
    • Related Report
      2010 Final Research Report
  • [Presentation] キチンオリゴ糖応答性の改変によるイネの菌類病抵抗性増強の試み2011

    • Author(s)
      香西雄介, ら
    • Organizer
      日本植物生理学会
    • Place of Presentation
      講演要旨集のみ
    • Year and Date
      2011-03-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] イネ剥離葉鞘裏面細胞を用いたいもち病菌感染初期における細胞内構造変化のリアルタイムイメージング法の構築2011

    • Author(s)
      望月進, ら
    • Organizer
      日本植物病理学会
    • Place of Presentation
      講演要旨集のみ
    • Year and Date
      2011-03-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] イネの持続的病害抵抗性の増強を目指した宿主-病原菌相互作用の研究2010

    • Author(s)
      西澤洋子
    • Organizer
      植物科学シンポジウム
    • Place of Presentation
      東京都品川コクヨホール
    • Year and Date
      2010-12-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] キチンエリシター受容シグナルの変換によるイネいもち病抵抗性増強の試み2010

    • Author(s)
      香西雄介, ら
    • Organizer
      日本農芸化学会
    • Place of Presentation
      千葉大(松戸)
    • Year and Date
      2010-10-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] キチンエリシター受容シグナルの改変によるイネいもち病抵抗性増強の試み2010

    • Author(s)
      香西雄介, ら
    • Organizer
      日本植物病理学会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2010-04-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] いもち病菌感染初期におけるイネの細胞内構造変化の可視化2010

    • Author(s)
      望月進, ら
    • Organizer
      日本植物病理学会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2010-04-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] エリシターにより発現誘導されるイネ膜結合型ubiquitin ligase EL5と相互作用するタンパク質の解析2010

    • Author(s)
      近藤勝彦・小岩井花恵・望月進・岸本久太郎・加藤悦子・南栄一・西澤洋子
    • Organizer
      日本育種学会第117回講演会
    • Place of Presentation
      京都
    • Year and Date
      2010-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] エリシターにより発現誘導されるイネ膜結合型ubiquitin ligase EL5と相互作用するタンパク質の解析2010

    • Author(s)
      近藤勝彦、小岩井花恵、望月進、岸本久太郎、加藤悦子、南栄一、西澤洋子
    • Organizer
      日本育種学会第117回講演会
    • Place of Presentation
      京都大学
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Defense mechanisms mediated by chitin in rice-blast interactions2010

    • Author(s)
      Yoko Nishizawa, Susumu Mochizuki, Ken-ichiro Saitoh, Kyutaro Kishimoto, Yusuke Kouzai, Eiichi Minami
    • Organizer
      10th Japan-US seminar
    • Place of Presentation
      オレゴン州立大学(アメリカ合衆国)
    • Year and Date
      2010-01-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Imaging analysis on the localization of surface-exposed chitin and rice cells during rice-Magnaporthe oryzae interactions2010

    • Author(s)
      Susumu Mochizuki, Ken-ichiro Saitoh, Eiichi Minami, Yoko Nishizawa
    • Organizer
      10th Japan-US seminar
    • Place of Presentation
      オレゴン州立大学(アメリカ合衆国)
    • Year and Date
      2010-01-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] イネのユビキチンリガーゼEL5は側根形成と細胞死を制御する2009

    • Author(s)
      望月進・岸本久太郎・近藤勝彦・中島恵美・倉野洋子・南栄一・西澤洋子
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-23
    • Related Report
      2010 Final Research Report
  • [Presentation] Rice EL5, an unstable RING-H2 type E3, protects cells from nitrate-related stress.2008

    • Author(s)
      Y.Nishizawa, E.Katoh, E.Minami
    • Organizer
      ZOMES第5回国際シンポジウム
    • Place of Presentation
      横浜
    • Year and Date
      2008-11-13
    • Related Report
      2010 Final Research Report
  • [Presentation] キチン受容シグナルをHR細胞死誘導シグナルに変換するキメラ受容体創出の試み2008

    • Author(s)
      岸本久太郎・賀来華江・渋谷直人・南栄一・西澤洋子
    • Organizer
      平成20年度植物感染生理談話会
    • Place of Presentation
      茨城県大子町
    • Year and Date
      2008-08-07
    • Related Report
      2010 Final Research Report
  • [Book] (APS Press) Defense mechanisms mediated by chitin in rice-blast interactions.2011

    • Author(s)
      Y.Nishizawa, S.Mochizuki, K.Saitoh, K.Kishimoto, Y.Kouzai, E.Minami
    • Publisher
      in Genome-enabled Analysis of Plant-Pathogen Interactions.
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] ユビキチン依存型タンパク質分解系を利用したタンパク分解システム2010

    • Inventor(s)
      加藤悦子、加藤静恵、角田由紀、赤木香予、南栄一、西澤洋子
    • Industrial Property Rights Holder
      農業生物資源研究所
    • Acquisition Date
      2010-03-05
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] イネの側根を発達させる方法2009

    • Inventor(s)
      西澤洋子、望月進、南栄一
    • Industrial Property Rights Holder
      農業生物資源研究所
    • Industrial Property Number
      2009-053434
    • Filing Date
      2009-03-06
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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