• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular physiological studies on insect excretion and water regulation and its application -physiology of aquaporin and its disorder-

Research Project

Project/Area Number 20380035
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionTottori University

Principal Investigator

AZUMA Masaaki  Tottori University, 大学院・連合農学研究科, 教授 (20175871)

Co-Investigator(Kenkyū-buntansha) TSUMUKI Hisaaki  岡山大学, 資源生物科学研究所, 教授 (60033255)
IZUMI Yohei  岡山大学, 資源生物科学研究所, 技術専門職員 (10457210)
HIRAYAMA Chikara  独立行政法人農業生物資源研究所, 昆虫ー昆虫・植物問相互作用研究ユニット, 主任研究員 (90370676)
HORI Hidetaka  新潟大学, 自然科学系, 教授 (00293241)
SHIOMI Kunihiro  信州大学, 繊維学部, 准教授 (70324241)
ISHIDA Yuko  神戸大学, 大学院・理学研究科, GCOEリサーチアソシエイト (90509861)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2008: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Keywords浸透圧調節 / 原形質膜 / 乾燥耐性 / 害虫制御 / アクアポリン / V-ATPase / プロトンポンプ / カイコ / ナシヒメシンクイ / クロキンバエ / 昆虫感覚子 / ニカメイガ / 休眠
Research Abstract

Epithelial and cellular mechanisms in water transport has been explored over 50 years. Knowledge on the molecular aspects of water regulation in living organisms has greately increased in recent years. Water transport across the plasma membrane depends on the presence of water channel-aquaporin (AQP). Studies on water transport itself in insect epithelia is so far lacking, although the movement of ions and water are inextricably linked. There are still few physiological studies on the multiple functions of AQP in insect epithelia. This project set out to investigate the present status on our understanding of insect aquaporins and the recent advances in the molecular and cellular roles of caterpillar's AQP.
Currently, all insect AQPs are water-specific except for the caterpillar's AQP, which possess the other isoform which enables to transport glycerol and urea (aquaglyceroporin : GLP). Water-specific AQPs in insects are classified into two closely related subfamilies, called DRIP (Drosophila Intrinsic Protein) and PRIP (Pyrocoelia rufa Intrinsic Protein) phylogenetically. Insect AQPs in very recent reports fall into DRIP or PRIP. In the silkworm, Bombyx mori, we have identified these three types, i.e., AQP-Bom1 as DRIP-type, Bom2 as GLP-type, Bom3 as PRIP-type. Caterpillars (solid/plant feeder) are normally endurable against drought and starvation compare to the liquid/plant feeders such as a leafhopper. Therefore, these three AQPs of B. mori, collaborate to maintain not only water balance, but also excretion in silkworm larvae.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (30 results)

All 2011 2010 2009 2008 Other

All Journal Article (14 results) (of which Peer Reviewed: 13 results) Presentation (9 results) Book (6 results) Remarks (1 results)

  • [Journal Article] Investigation of physicochemical condition to stabilize phosphatidylcholine-liposome enclosing fluorescent calcein and its exploitation for analysis of pore formation with Cry1A toxins of Bacillus thuringiensis.2010

    • Author(s)
      Haginoya, K., Thangavel, V., Pandian, G.N., Tomimoto, K., Shitomi, Y., Azuma, M., Angsuthanasombat, C., Hori, H
    • Journal Title

      Appl.Entomol. Zool. 45

      Pages: 477-488

    • NAID

      130000327188

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Investigation of physicochemical condition to stabilize phosphatidylcholine-liposome enclosing fluorescent calcein and its exploitation for analysis of pore formation with of Cry1A toxins of Bacillus thuringiensis.2010

    • Author(s)
      Kohsuke Haginoya
    • Journal Title

      Applied Entomology and Zoology

      Volume: 45 Pages: 477-488

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] cDNA cloning of aquaporin gene expressed in the digestive tract of the Formosan subterranean termite, Coptotermes formosanus Shiraki (Isoptera ; Rhinotermitidae).2009

    • Author(s)
      Kambara, K., Takematsu, Y., Azuma, M., Kobayashi, J.
    • Journal Title

      Appl.Entomol.Zool. 44

      Pages: 315-321

    • NAID

      130000143010

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aquaporin and aquaglyceroporin in silkworms, differently expressed in the hindgut and midgut of Bombyx mori.2009

    • Author(s)
      Kataoka, N., Miyake, S., Azuma, M.
    • Journal Title

      Insect Mol.Biol. 18

      Pages: 303-314

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization of aquaporin and aquaglyceroporin in the alimentary canal of Grapholita molesta (the oriental fruit moth)-comparison with Bombyx mori aquaporins.2009

    • Author(s)
      Kataoka, N., Miyake, S., Azuma, M.
    • Journal Title

      J.Insect Biotechnol.Sericol. 78

      Pages: 81-90

    • NAID

      10026131796

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] cDNA cloning of aquaporin gene expressed in the digestive tract of the Formosan subterranean termite, Coptotermes formosanus Shiraki (Isoptera ; Rhinotermitidae).2009

    • Author(s)
      Kohei Kambara
    • Journal Title

      Applied Entomology and Zoology 44

      Pages: 315-321

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aquaporin and aquaglyceroporin in silkworms, differently expressed in the hindgut and midgut of Bombyx mori.2009

    • Author(s)
      Naoya Kataoka
    • Journal Title

      Insect Molecular Biology 18

      Pages: 303-314

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization of aquaporin and aquaglyceroporin in the alimentary canal of Grapholita molesta (the oriental fruit moth)-comparison with Bombyx mori aquaporins.2009

    • Author(s)
      Naoya Kataoka
    • Journal Title

      J.Insect Biotechnology and Sericology 78

      Pages: 81-90

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      三宅誠司
    • Journal Title

      絹糸腺でpHと水分を調節するしくみ((財)衣笠会 編)(為国印刷(株)) (ISBNなし,非売品)

      Pages: 166-169

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Developmental expression and the physiological role of aquaporin in the silk gland of Bombyx mori.2008

    • Author(s)
      Miyake, S., Azuma, M.
    • Journal Title

      J.Insect Biotechnol.S ericol. 77

      Pages: 87-93

    • NAID

      10021221111

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acidification of the silk gland in Bombyx mori and Samia cynthia ricini in relation to the distribution of H^+-translocating vacuolar-type ATPase.2008

    • Author(s)
      Seiji Miyake
    • Journal Title

      J. Insect Biotechnology and Sericology 77

      Pages: 9-16

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Developmental expression and the physiological role of aquaporin inthe silk gland of Bombyx mori.2008

    • Author(s)
      Seiji Miyake
    • Journal Title

      J. Insect Biotechnology and Sericology 77

      Pages: 87-93

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regioselective formation of quercetin 5-O-glucoside from orally administered quercetin in the silkworm, Bombyx mori.2008

    • Author(s)
      Chikara Hirayama
    • Journal Title

      Phytochemistry 69

      Pages: 1141-1149

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes in the expression of soluble and integral-membrane trehalase in the midgut during metamorphosis in Bombyx mori.2008

    • Author(s)
      Kanako Mitsumasu
    • Journal Title

      Zoological Science 25

      Pages: 693-698

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] カイコ卵巣の発育過程におけるアクアポリン(水チャネル)の役割2011

    • Author(s)
      丸山麻理弥・永江知音・東政明
    • Organizer
      蚕糸・昆虫機能利用学術講演会~日本蚕糸学会第81回大会
    • Place of Presentation
      東京都
    • Related Report
      2010 Final Research Report
  • [Presentation] 後腸とそれに並走するマルピーギ管におけるアクアポリンの局所的分布とその機能2010

    • Author(s)
      東政明
    • Organizer
      平成22年度蚕糸・昆虫機能利用学術講演会日本蚕糸学会第80回記念大会
    • Place of Presentation
      信州大学
    • Year and Date
      2010-04-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] 後腸とそれに並走するマルピーギ管におけるアクアポリンの局所的分布とその機能2010

    • Author(s)
      東政明・丸山麻理弥
    • Organizer
      蚕糸・昆虫機能利用学術講演会~日本蚕糸学会第80回記念大会
    • Place of Presentation
      上田市
    • Related Report
      2010 Final Research Report
  • [Presentation] カイコ幼虫の水バランスにはたらく後腸アクアポリンの抗ペプチド抗体を用いた分析2009

    • Author(s)
      丸山麻理弥
    • Organizer
      日本蚕糸学会第75回関西支部・第65回九州支部合同大会 蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      山口大学
    • Year and Date
      2009-11-13
    • Related Report
      2009 Annual Research Report
  • [Presentation] 鱗翅目幼虫のアクアポリンとアクアグリセロポリンの構造的特徴と機能的解析2009

    • Author(s)
      東 政明
    • Organizer
      平成21年度蚕糸・昆虫機能利用学術講演会日本蚕糸学会第79回大会
    • Place of Presentation
      東京農工大学・農学部
    • Year and Date
      2009-03-21
    • Related Report
      2008 Annual Research Report
  • [Presentation] カイコ幼虫の水バランスにはたらく後腸アクアポリンの抗ペプチド抗体を用いた分析2009

    • Author(s)
      丸山麻理弥・東政明
    • Organizer
      日本蚕糸学会第75回関西支部・第65回九州支部合同大会蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      山口市
    • Related Report
      2010 Final Research Report
  • [Presentation] Physiology of aquaporins in lepidopteran caterpillars.2009

    • Author(s)
      東政明
    • Organizer
      3rd International Symposium on the Environmental Physiology of Ectotherms and Plants (ISEPEP3)
    • Place of Presentation
      Tsukuba, Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] 鱗翅目幼虫のアクアポリンとアクアグリセロポリンの構造的特徴と機能的解析2009

    • Author(s)
      片岡直也・三宅誠司・東政明
    • Organizer
      蚕糸・昆虫機能学術講演会~日本蚕糸学会第79回大会
    • Place of Presentation
      東京都
    • Related Report
      2010 Final Research Report
  • [Presentation] Molecular cloning, tissue and cellular characterization of two isoforms of aquaporin in silkworms, differently expressed in the hindgut and midgut of Bombyx mori.2008

    • Author(s)
      三宅誠司, 東政明
    • Organizer
      International Symposium on Insect Midgut Biology, South China Normal Univ.
    • Place of Presentation
      Guangzhou, China
    • Related Report
      2010 Final Research Report
  • [Book] 水チャネル(アクアポリン)のはたらき.昆虫の低温耐性-その仕組みと調べ方-(積木久明編)2010

    • Author(s)
      東政明
    • Publisher
      岡山大学出版会,岡山.
    • Related Report
      2010 Final Research Report
  • [Book] 昆虫の水代謝.からだと水の事典(佐々木成・石橋賢一編)2010

    • Author(s)
      東政明
    • Publisher
      朝倉書店,東京
    • Related Report
      2010 Final Research Report
  • [Book] アクアポリン-水チャネル-のはたらき(昆虫の低温耐性-その仕組みと調べ方-,積木久明・田中一裕・後藤三千代編,分担執筆)2010

    • Author(s)
      東政明
    • Publisher
      岡山大学出版会(ISBN978-4-904228-14-2)
    • Related Report
      2010 Annual Research Report
  • [Book] 虫たちが語る生物学の未来(絹糸腺でpHと水分を調節するしくみ)2009

    • Author(s)
      三宅誠司・東政明
    • Publisher
      為国印刷(株)京都(財)衣笠会編
    • Related Report
      2010 Final Research Report
  • [Book] 昆虫の水代謝からだと水の事典(佐々木成・石橋賢一編)2008

    • Author(s)
      東 政明
    • Publisher
      朝倉書店(ISBN 978-4-254-30094-9)
    • Related Report
      2008 Annual Research Report
  • [Book] ニカメイガ幼虫の凍結障害の発生と回避機構耐性の昆虫学(田中誠二・小滝冨美・田中一裕編)2008

    • Author(s)
      積木久明
    • Publisher
      東海大学出版会(ISBN 978-4-486-01790-5)
    • Related Report
      2008 Annual Research Report
  • [Remarks] 研究グループは,2009年1月6日 (於 : 岡山大・資源生物研) に公開で研究発表討論会を開催した (参加者約30名)。

    • Related Report
      2008 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi