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Research on Photosynthesis Improvement in Transgenic Rice Transformed with RBCS

Research Project

Project/Area Number 20380041
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionTohoku University

Principal Investigator

MAKINO Amane  東北大学, 大学院・農学研究科, 教授 (70181617)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2010: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2008: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Keywordsルビスコ / イネ / 光合成 / バイオマス生産 / RBCS組換体 / バイオマス
Research Abstract

Photosynthesis improvent and the enhancement of biomass production were examined in transgenic rice transformed with RBCS. Overexpression of RBCS led an increase in Rubisco proptein, but did not result in increases in photosynthesis and plant biomass. However, such transgenic rice were grown better under conditions of low CO2 or low temperatures. For RBCS muligene family, OsRBCS2 to OsRBCS5, which are located on Chromosome 12, are expressed substantially, and were not compensated for each other. Each RBCS-RNAi plant had higher photosynthesis only under conditions of elevated [CO2].

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (56 results)

All 2011 2010 2009 2008 Other

All Journal Article (30 results) (of which Peer Reviewed: 30 results) Presentation (20 results) Book (4 results) Remarks (2 results)

  • [Journal Article] Photosynthesis, grain yield and N utilization in rice and wheat2011

    • Author(s)
      Makino A
    • Journal Title

      Plant Physiol

      Volume: 155 Pages: 125-129

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] New insight into photosynthetic acclimation to elevated CO2 : The role of leaf nitrogen and ribulose-1, 5-bisphosphate carboxylase/oxygenase in rice leaves.2011

    • Author(s)
      Seneweera S, Makino A, Hirotsu N, Norton R, Suzuki Y
    • Journal Title

      Environ. Exp. Bot

      Volume: 71 Pages: 128-136

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The rate-limiting step for CO2 assimilation at different temperatures is influenced by the leaf nitrogen content in several C3 crop species2011

    • Author(s)
      Yamori W, Nagai T and Makino A
    • Journal Title

      Plant Cell Environ

      Volume: 34 Pages: 764-777

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Photosynthesis, grain yield and N utilization in rice and wheat2011

    • Author(s)
      Makino A
    • Journal Title

      Plant Physiol.

      Volume: 155 Pages: 125-129

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differences in Rubisco content and its synthesis and degradation in leaves at different positions in Eucalyptus globulus seedlings2010

    • Author(s)
      Suzuki Y, Kihara-Doi T, Kawazu T, Miyake C and Makino A
    • Journal Title

      Plant Cell Environ

      Volume: 33 Pages: 1314-1323

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increased grain yield and biomass allocation in rice under cool night temperature2010

    • Author(s)
      Kanno K and Makino A
    • Journal Title

      Soil Sci Plant Nutr

      Volume: 56 Pages: 412-417

    • NAID

      110008144337

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protein turnover in grass leaves2010

    • Author(s)
      Irving LJ, Suzuki Y, Ishida H and Makino A
    • Journal Title

      Advances in Botanical Research

      Volume: 54 Pages: 139-182

    • Related Report
      2010 Annual Research Report 2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The autophagic degradation of chloroplasts via Rubisco-containing bodies is specially linked to leaf carbon status but not nitrogen status in Arabidopsis2010

    • Author(s)
      Izumi M, Wada S, Makino A and Ishida H
    • Journal Title

      Plant Physiol

      Volume: 154 Pages: 1196-1209

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical aspects associated with in vivo RbcL fragmentation by reactive oxygen species under chilling-light conditions2010

    • Author(s)
      Nakano, et al., 代表者、牧野を含む, 他4名
    • Journal Title

      Plant Biology

      Volume: 12 Pages: 35-45

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An increase in grain yield and biomass allocation in rice under cool night temperature2010

    • Author(s)
      Kanno K, Makino A
    • Journal Title

      Soil Sci.Plant Nutr.

      Volume: 56 Pages: 412-417

    • NAID

      110008144337

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The autophagic degradation of chloroplasts via Rubisco-containing bodies is specially linked to leaf carbon status but not nitrogen status in Arabidopsis2010

    • Author(s)
      Izumi M, Wada S, Makino A, Ishida H
    • Journal Title

      Plant Physiol.

      Volume: 154 Pages: 1196-1209

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differences in Rubisco content and its synthesis and degradation in leaves at different positions in Eucalyptus globulus seedlings2010

    • Author(s)
      Suzuki Y, Kihara-Doi T, Kawazu T, Miyake C, Makino A
    • Journal Title

      Plant Cell Environ.

      Volume: 33 Pages: 1314-1323

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical aspects associated with in vivo RbcL fragmentation by reactive oxygen species under chilling-light conditions.2010

    • Author(s)
      Nakano, et al.(代表者、牧野を含む他4名)
    • Journal Title

      Plant Biology 12

      Pages: 35-45

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An increase in grain yield and biomass allocation in rice under cool night temperature2010

    • Author(s)
      Kanno K, Makino A
    • Journal Title

      Soil Sci.Plant Nutr. 56(In press)

    • NAID

      110008144337

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High night-temperature stimulates photosynthesis, biomass production and plant growth during the vegetative stage of rice plants2009

    • Author(s)
      Kanno K, Mae T and Makino A
    • Journal Title

      Soil Sci. Plant Nutr

      Volume: 55 Pages: 124-131

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Autophagy plays a role in chloroplast degradation during senescence in individually darkened leaves2009

    • Author(s)
      Wada S, Ishida H, Izumi M, Yoshimoto K, Ohsumi Y, Mae T and Makino A
    • Journal Title

      Plant Physiol

      Volume: 149 Pages: 885-893

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rubisco content and photosynthesis at different positions in transgenic rice with an overexpression of RBCS2009

    • Author(s)
      Suzuki Y, Miyamoto, T, Yoshizawa R, Mae, T and Makino A
    • Journal Title

      Plant Cell Environ

      Volume: 32 Pages: 417-427

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Photosynthesis research in plant science2009

    • Author(s)
      Tanaka A and Makino A
    • Journal Title

      Plant Cell Physiol

      Volume: 50 Pages: 681-683

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differences between rice and wheat in temperature responses of photosynthesis and plant growth2009

    • Author(s)
      Nagai T and Makino A
    • Journal Title

      Plant Cell Physiol

      Volume: 50 Pages: 744-755

    • NAID

      130006992371

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differences in expression of the RBCS multigene family and Rubisco protein content in various rice plant tissues at different growth stages2009

    • Author(s)
      Suzuki Y, Nakabayashi K, Yoshizawa R, Mae T and Makino A
    • Journal Title

      Plant Cell Physiol

      Volume: 50 Pages: 1851-1855

    • NAID

      10027343957

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The analysis of factors affecting the creation of Rubisco-containing bodies (RCBs), a specific autophagic process for chloroplast degradation in plants.2009

    • Author(s)
      Izumi M, Ishida H, Makino A
    • Journal Title

      Autophagy

      Volume: 5 Pages: 914-914

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rubisco content and photosynthesis at different positions in transgenic rice with an overexpression of RBCS.2009

    • Author(s)
      Suzuki, et al(代表者、牧野を含む他4名)
    • Journal Title

      Plant Cell Environ. 32

      Pages: 417-427

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differences between rice and wheat in temperature responses of photosynthesis and plant growth.2009

    • Author(s)
      Nagai T, Makino A
    • Journal Title

      Plant Cell Physiol. 50

      Pages: 744-755

    • NAID

      130006992371

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differences in expression of the RBCS multigene family and Rubisco protein content in various rice plant tissues at different growth stages.2009

    • Author(s)
      Suzuki, et al(代表者、牧野を含む他4名)
    • Journal Title

      Plant Cell Physiol. 50

      Pages: 1851-1855

    • NAID

      10027343957

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Autophagy plays a role in chloroplast degradation during senescence in individually darkened leaves2009

    • Author(s)
      Wada et.al.(研究代表者牧野を含む他6名)
    • Journal Title

      Plant Physiology (IF = 6.376) 149

      Pages: 885-893

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rubisco content and photosynthesis at different positions in transgenic rice with an overexpression of RBCS2009

    • Author(s)
      Suzuki Y, Miyamoto T, Yosh-izawa R, Mae T, Makino A
    • Journal Title

      Plant, Cell and Environment (IF = 4.497) 32

      Pages: 417-427

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High night-temperature stimulates photosynthesis, biomass production and plant growth during the vegetative stage of rice2009

    • Author(s)
      Kanno K, Mae T, Makino A
    • Journal Title

      Soil Sci. Plant Nutr. (IF = 1.084) 55

      Pages: 124-131

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differences between rice and wheat in temperature responses of photosynthesis and plant growth2009

    • Author(s)
      Nagai T, Makino A
    • Journal Title

      Plant and Cell Physiology (IF = 3.654) 50(In press)

    • NAID

      130006992371

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNA extraction from various recalcitrant plant tissues with a cethyltrimethylammonium bromide-containing buffer followed by acid guanidium thiocyanate-phenol chloroform treatment2008

    • Author(s)
      Suzuki Y, Mae T and Makino A
    • Journal Title

      Biosci, Biotechnol. Biochem

      Volume: 72 Pages: 1951-1953

    • NAID

      10027530447

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mobilization of Rubisco and stromal-localized fluorescent proteins of chloroplasts to the vacuole by an ATG gene-dependent autophagic process2008

    • Author(s)
      Ishida H, Yoshimoto K, Izumi M, Reisen D, Yano Y, Makino A, Ohsumi Y, Hanson M and Mae T
    • Journal Title

      Plant Physiol

      Volume: 148 Pages: 142-155

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] イネの生産性と個葉光合成2010

    • Author(s)
      牧野周
    • Organizer
      日本光合成学会シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2010-05-29
    • Related Report
      2010 Final Research Report
  • [Presentation] イネにおいてOsRBCS multigene familyの個別発現抑制は葉身Rubisco量を減少させる2010

    • Author(s)
      小川瞬(代表者、牧野を含む他4名)
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] デンプン代謝変異がRubisco-containing body(RCB)の形成に及ぼす影響の解析2010

    • Author(s)
      泉正範、石田宏幸、牧野周
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] イネにおいてOsRBCS multigene familyの個別発現抑制は葉身Rubisco量を減させる2010

    • Author(s)
      小川瞬ら(代表者、牧野を含む他4名)
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      熊本
    • Related Report
      2010 Final Research Report
  • [Presentation] OsRBCS3を発現抑制したイネの大気CO2分圧下での個体成長2010

    • Author(s)
      菅野圭一ら(代表者、牧野を含む他3名)
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      熊本
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] デンプン代謝変異がRubisco-containing body (RCB)の形成に及ぼす影響の解析2010

    • Author(s)
      泉正範ら(代表者、牧野を含む他1名)
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      熊本
    • Related Report
      2010 Final Research Report
  • [Presentation] シロイヌナズナ個別暗処理葉におけるオートファジー葉緑体分解機構2010

    • Author(s)
      和田慎也ら(代表者、牧野を含む他4名)
    • Organizer
      日本土壌肥料学会札幌大会
    • Place of Presentation
      札幌
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 異なるCO_2分圧下でのOsRBCS3ノックダウンイネの乾物生産と窒素利用2010

    • Author(s)
      菅野圭一ら(代表者、牧野を含む他2名)
    • Organizer
      日本土壌肥料学会札幌大会
    • Place of Presentation
      札幌
    • Related Report
      2010 Final Research Report
  • [Presentation] 葉位の異なるEucalyptus globulus葉におけるRubiscoの遺伝子発現2010

    • Author(s)
      鈴木雄二ら(代表者、牧野を含む他4名)
    • Organizer
      日本土壌肥料学会札幌大会
    • Place of Presentation
      札幌
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Rubiscoタンオーバーと窒素栄養2010

    • Author(s)
      鈴木雄二ら(代表者、牧野を含む他2名)
    • Organizer
      日本土壌肥料学会札幌大会
    • Place of Presentation
      札幌
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 異なるCO2分圧下でのOsRBCS3ノックダウンイネの乾物生産と窒素利用2010

    • Author(s)
      菅野圭一、鈴木雄二、小川瞬、牧野周
    • Organizer
      日本土壌肥料学会札幌大会
    • Place of Presentation
      札幌
    • Related Report
      2010 Annual Research Report
  • [Presentation] イネの個葉光合成と生産性2009

    • Author(s)
      牧野周
    • Organizer
      日本光合成学会シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2009-05-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] RBCS遺伝子を過剰発現した形質転換体イネのRubisco量2009

    • Author(s)
      鈴木雄二ら(代表者、牧野を含む他4名)
    • Organizer
      光合成および個体生育日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] イネRBCS multigene familyの生育別・器官別の発現の違いについて2009

    • Author(s)
      中林香(代表者、牧野を含む他4名)
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] Nitrogen oscillation in the tiller axes of grassas A role in leaf production2009

    • Author(s)
      Irvingら(代表者、牧野を含む他2名)
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] シロイヌナズナ切離葉におけるRubisco-containing body (RCB)の形成に影響を及ぼす要因2009

    • Author(s)
      泉正範ら(代表者、牧野を含む他2名)
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] 刃ROS遺伝子を過剰発現した形質転換体イネのRubisco量、光合成および個体生育2009

    • Author(s)
      鈴木ら(牧野を含む他4名)
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2008 Annual Research Report
  • [Presentation] イネRBCS multigene familyの生育別・器官別の発現の違いについて2009

    • Author(s)
      中林ら(牧野を含む他4名)
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2008 Annual Research Report
  • [Presentation] Nitrogen oscillation in the tiller axes of grassas A role in leaf production2009

    • Author(s)
      Irvingら(牧野を含む他2名)
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2008 Annual Research Report
  • [Presentation] シロイヌナズナ切離葉におけるRubisco-containing body(RCB)の形成に影響を及ぼす要因2009

    • Author(s)
      泉ら(牧野を含む他2名)
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2008 Annual Research Report
  • [Book] Nitrogen utilization, growth and yield in rice plants. In Nitrogen Assimilation in Plants : Chapter 172010

    • Author(s)
      Mae T and Makino A
    • Publisher
      Eds by Ohyama T and Sueyoshi K
    • Related Report
      2010 Final Research Report
  • [Book] Rubisco turnover and photosynthesis in a leaf. In Nitrogen Assimilation in Plants : Chapter 172010

    • Author(s)
      Ishida H, Suzuki Y and Makino A
    • Publisher
      Eds by Ohyama T and Sueyoshi K
    • Related Report
      2010 Final Research Report
  • [Book] Nitrogen utilization, growth and yield in rice plants. In Nitrogen Assimilation in Plants : Chapter 17(Eds by Ohyama T and Sueyoshi K)2010

    • Author(s)
      Mae T, Makino A
    • Publisher
      Research Signpost
    • Related Report
      2010 Annual Research Report
  • [Book] Rubisco turnover and photosynthesis in a leaf. In Nitrogen Assimilation in Plants : Chapter 17(Eds by Ohyama T and Sueyoshi K)2010

    • Author(s)
      Ishida H, Suzuki Y, Makino A
    • Publisher
      Research Signpost
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.agri.tohoku.ac.jp/syokuei/index-j.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.agri.tohoku.ac.jp/syokuei/index-j.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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