Functional analysis of bacterial actin-like cytoskeletal proteins by using inhibitors

• Project/Area Number: 20380047
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: WACHI Masaaki Tokyo Institute of Technology, 大学院・生命理工学研究科, 准教授 (90192822)
• Co-Investigator(Kenkyū-buntansha): TAKADA Ayako 東京工業大学, 技術部, 技術職員 (20401565)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2009: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2008: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
• Keywords: 微生物学 / アクチン様細胞骨格タンパク質 / MreB / A22 / 阻害剤 / PBP1B / 溶菌 / 蛍光標識誘導体 / アクチン様細胞骨格 / 転写

Research Abstract

It was shown that MreB helical structures were clearly visualized by staining with a newly synthesized anthracene-labeled A22 derivative in living E.coli cells. This provides the simple method observing bacterial actin-like cytoskeletons. We found that A22 induced cell lysis in E.coli mrcB mutant cells lacking PBP1B, one of the major penicillin-binding proteins. mreB(ts) mrcB double mutant lysed immediately upon temperature up-shift. These results indicate that simultaneous defects of MreB and PBP1B cause cell lysis.

Research Products

• [Journal Article] A requirement of ToIC and MDR efflux pumps for acid adaptation and GadAB induction in Escherichia coli. (2011)
  • Author(s): Deininger KN, Horikawa A, Kitko RD, Tatsumi R, Rosner JL, Wachi M, Slonczewski JL.
  • Journal Title: PLoS One. 6
  • Peer Reviewed
• [Journal Article] A role of the cspA gene encoding a mycolyltransferase in the growth under alkaline conditions of Corynebacterium glutamicum. (2010)
  • Author(s): Takeshita R, Ito H, Wachi M
  • Journal Title: Biosci Biotechnol Riochem. 74 Pages: 1617-1623
  • NAID: 10027558199
  • Peer Reviewed
• [Journal Article] Requirement of de novo synthesis of the OdhT protein in penicillin-induced glutamate production by Corynebacterium glutamicum. (2010)
  • Author(s): Kim J, Fukuda H, Hirasawa T, Nagahisa K, Nagai K, Wachi M, Shimizu H.
  • Journal Title: Appl Microbiol Biotechnol. 86 Pages: 911-920
  • Peer Reviewed
• [Journal Article] Structure of the heme biosynthetic Pseudomonas aeruginosa porphobilinogen synthase in complex with the antibiotic alaremycin. (2010)
  • Author(s): Heinemann IU, Schulz C, Schubert WD, Heinz DW, Wang YG, Kobayashi Y, Awa Y, Wachi M, Jahn D, Jahn M.
  • Journal Title: Antimicrob Agents Chemother. 54 Pages: 267-272
  • Peer Reviewed
• [Journal Article] 大腸菌RNaseGによる解糖系び制御 (2010)
  • Author(s): 和地正明
  • Journal Title: バイオサイエンスとインダストリー 68 Pages: 34-38
• [Journal Article] A role of the cspA gene encoding a mycolyltransferase in the growth under alkaline conditions of Corynebacterium glutamicum (2010)
  • Author(s): Takeshita R, Ito H, Wachi M
  • Journal Title: Biosci Biotechnol Biochem. Volume: 74 Pages: 1617-1623
  • NAID: 10027558199
  • Peer Reviewed
• [Journal Article] Requirement of de novo synthesis of the OdhI protein in penicillin-induced glutamate production by Corynebacterium glutamicum (2010)
  • Author(s): Kim, J.
  • Journal Title: Appl Microbiol Biotechnol. 86 Pages: 911-920
  • Peer Reviewed
• [Journal Article] Structure of the heme biosynthetic Pseudomonas aeruginosa porphobilinogen synthase in complex with the antibiotic alaremycin (2010)
  • Author(s): Heinemann, I.U.
  • Journal Title: Antimicrob Agents Chemother. 54 Pages: 267-272
  • Peer Reviewed
• [Journal Article] 大腸菌RNase Gによる解糖系の制御 (2010)
  • Author(s): 和地正明
  • Journal Title: バイオサイエンスとインダストリー 63 Pages: 34-38
  • NAID: 10026194999
• [Journal Article] The Corynebacterium glutamicum NCg12281 gene encoding an Base E/G family endoribonuclease can complement the Escherichia coli rng : cat mutation but not the rne-1 mutation. (2009)
  • Author(s): Maeda T, Sakai T, Wachi M
  • Journal Title: Riosci Biotechnol Riochem. 73 Pages: 2281-2286
  • Peer Reviewed
• [Journal Article] The Corynebacterium glutamicum NCg12281 gene encoding an RNase E/G family endoribonuclease can complement the Escherichia coli rng::cat mutation but not the rne-1 mutation (2009)
  • Author(s): Maeda, T.
  • Journal Title: Biosci Biotechnol Biochem. 73 Pages: 2281-2286
  • Peer Reviewed
• [Journal Article] Structural and functional characterization of the LIdR from Corynebacterium glutamicum : a transcriptional repressor involved in L-lactate and sugar utilization. (2008)
  • Author(s): Gao YG, Suzuki H, Itou H, Zhou Y, Tanaka Y, Wachi M, Watanabe N, Tanaka I, Yao M.
  • Journal Title: Nucleic Acids Res. 36 Pages: 7110-7123
  • Peer Reviewed
• [Journal Article] Distinctroles of two anaplerotic pathways in glutamate production induced by biotin limitation in Corynebacterium glutamicum. (2008)
  • Author(s): Sato H, Orishimo K, Shirai T, Hirasawa T, Nagahisa K, Shimizu H, Wachi M
  • Journal Title: J Biosci Bioeng. 106 Pages: 51-58
  • Peer Reviewed
• [Journal Article] ToIC-dependent exclusion of porphyrins in Rscherichia coli. (2008)
  • Author(s): Tatsumi R, Wachi M
  • Journal Title: J Bacterial. 190 Pages: 6228-6233
  • Peer Reviewed
• [Journal Article] Anti-infectious effect of S-benzylisothiourea compound A22, which inhibits the actin-like protein, MreB, in Shigella flexneri. (2008)
  • Author(s): Noguchi N, Yanagimoto K, Nakaminami H, Wakabayashi M, Iwai N, Wachi M, Sasatsu M.
  • Journal Title: Biol Pharm Bull. 31 Pages: 1327-1332
  • NAID: 110006792097
  • Peer Reviewed
• [Journal Article] structural and functional characterization of the LldR from Corynebacterium glutamicum : a transcriptional repressor involved in L-lactate and sugar utilization. (2008)
  • Author(s): Gao, Y G.
  • Journal Title: Nucleic Acids Res. 36 Pages: 7110-7123
  • Peer Reviewed
• [Journal Article] Distinct roles of two anaplerotic pathways in glutamate production induced by biotin limitation in Corynebacterium glutamicum. (2008)
  • Author(s): Sato, H.
  • Journal Title: J. Biosci. Bioeng. 106 Pages: 51-58
  • Peer Reviewed
• [Journal Article] TolC-dependent exelusion of porphyrins in Escherichia coli. (2008)
  • Author(s): Tatsumi, R.
  • Journal Title: J. Bacteriol. 190 Pages: 6228-6233
  • Peer Reviewed
• [Journal Article] Anti-infectious effect of S-benzylisothiourea compound A22, which inhibits the actin-like protein, MreB, in Shigella flexneri (2008)
  • Author(s): Noguchi, N.
  • Journal Title: Biol. Pharm. Bull. 31 Pages: 1327-1332
  • Peer Reviewed
• [Presentation] Corynebacterium glutamicumのOdhIタンパク質の増殖における機能 (2010)
  • Author(s): 竹内さほ
  • Organizer: 第62回日本生物工学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-09-29
• [Presentation] 大腸菌RNase GのmRNA切断点認識機構について (2010)
  • Author(s): 伊藤和敬
  • Organizer: 第62回日本生物工学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-09-28
• [Presentation] RNase G変異株を用いたmRNAの安定化によるタンパク質過剰発現系 (2010)
  • Author(s): 和地正明
  • Organizer: 第62回日本生物工学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-09-27
• [Presentation] A new antibiotic alaremycin inhibits porphobilinogen synthase, the initial step of the porphyrin biosynthesis. (2010)
  • Author(s): 和地正明
  • Organizer: 11th International Symposium on the Genetics of Industrial Microorganisms
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2010-06-30
• [Presentation] A new antibiotic alaremycin inhibits porphobilinogen synthase, the initial step of the porphyrin biosynthesis (2010)
  • Author(s): Masaaki Wachi
  • Organizer: 11^<th> International Symposium on the Genetics of Industrial Microorganisms
  • Place of Presentation: Australia
  • Year and Date: 2010-06-30
• [Presentation] Functional analysis of the Corynebactrium glutamicum RNase E/G family endoribonulease (2010)
  • Author(s): Tomoya Maeda
  • Organizer: 11^<th> International Symposium on the Genetics of Industrial Microorganisms
  • Place of Presentation: Australia
  • Year and Date: 2010-06-30
• [Presentation] Simultaneous defects of MreB and PBP 1B cause cell lysis in Escherichia coli (2010)
  • Author(s): Chie Kosaka
  • Organizer: 11^<th> International Symposium on the Genetics of Industrial Microorganisms
  • Place of Presentation: Australia
  • Year and Date: 2010-06-30
• [Presentation] A role of the NCg11221 mechanosensitive channel in gluatmic acid production by Corynebacterium glutamicum (2010)
  • Author(s): Chikako Yamashita
  • Organizer: 11^<th> International Symposium on the Genetics of Industrial Microorganisms
  • Place of Presentation: Australia
  • Year and Date: 2010-06-30
• [Presentation] A role of NCg11220 in stress response of Corynebacterium glutamicum (2010)
  • Author(s): Kosuke Kumagai
  • Organizer: 11^<th> International Symposium on the Genetics of Industrial Microorganisms
  • Place of Presentation: Australia
  • Year and Date: 2010-06-29
• [Presentation] Inhibition of flagellation by the bacterial actin MreB inhibitor A22 (2010)
  • Author(s): Ayako Takada
  • Organizer: 11^<th> International Symposium on the Genetics of Industrial Microorganisms
  • Place of Presentation: Australia
  • Year and Date: 2010-06-28
• [Presentation] A role of the outer-membrane TolC protein in acid tolerance of Escherichia coli (2010)
  • Author(s): Akina Horikawa
  • Organizer: 11^<th> International Symposium on the Genetics of Industrial Microorganisms
  • Place of Presentation: Australia
  • Year and Date: 2010-06-28
• [Presentation] アラレマシンによるポルフィリン合成系ポルフォビリノーゲン合成酵素の阻害 (2010)
  • Author(s): 和地正明
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-30
• [Presentation] アラレマイシンによるポルフィリン合成系ポルフォビリノーゲン合成酵素の阻害 (2010)
  • Author(s): 和地正明
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-30
• [Presentation] アクチン様タンパク質MreBとペニシリン結合タンパク質PBP1Bの同時欠損による溶菌 (2010)
  • Author(s): 小坂智恵
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-28
• [Presentation] MreBとPBP1Bの同時欠損による大腸菌の溶菌 (2009)
  • Author(s): 坂庭友子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] 大腸菌RNase GのmRNA切断部位の認識について (2009)
  • Author(s): 伊藤和敬
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 2009-07-28
• [Presentation] Corynebacterium glutamicumのRnase E/G ファミリー酵素の機能解析 (2009)
  • Author(s): 前田智也
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟
  • Year and Date: 2009-07-28
• [Presentation] 細菌のアクチン様タンパク質阻害剤の開発とプローブ化 (2009)
  • Author(s): 岩井伯隆
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] アクチン様タンパク質MreB阻害剤A22を用いた桿菌形態形成機構の解析 (2009)
  • Author(s): 和地正明、高田綾子、岩井伯隆
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-13
• [Presentation] アクチン様タンパク質MreB阻害剤A22を用いた桿菌形態形成機構の解析 (2009)
  • Author(s): 和地正明
  • Organizer: 第8 2回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-13