Functional analysis of bacterial actin-like cytoskeletal proteins by using inhibitors
| Project/Area Number |
20380047
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Tokyo Institute of Technology |
|---|
Principal Investigator |
WACHI Masaaki Tokyo Institute of Technology, 大学院・生命理工学研究科, 准教授 (90192822)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAKADA Ayako 東京工業大学, 技術部, 技術職員 (20401565)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2009: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2008: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
|
|---|
| Keywords | 微生物学 / アクチン様細胞骨格タンパク質 / MreB / A22 / 阻害剤 / PBP1B / 溶菌 / 蛍光標識誘導体 / アクチン様細胞骨格 / 転写 |
|---|
| Research Abstract |
It was shown that MreB helical structures were clearly visualized by staining with a newly synthesized anthracene-labeled A22 derivative in living E.coli cells. This provides the simple method observing bacterial actin-like cytoskeletons. We found that A22 induced cell lysis in E.coli mrcB mutant cells lacking PBP1B, one of the major penicillin-binding proteins. mreB(ts) mrcB double mutant lysed immediately upon temperature up-shift. These results indicate that simultaneous defects of MreB and PBP1B cause cell lysis.
|
Report
(4 results)
Research Products
(39 results)
