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Structure/function relationship and cell surface localization mechanism of bacterial flagelin

Research Project

Project/Area Number 20380049
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionKyoto University

Principal Investigator

MURATA Kousaku  Kyoto University, 農学研究科, 教授 (90142299)

Co-Investigator(Kenkyū-buntansha) 橋本 渉  京都大学, 農学研究科 (30273519)
三上 文三  京都大学, 農学研究科 (40135611)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2008: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywordsフラジェリン / 鞭毛 / ペプチドグリカン加水分解酵素 / メタロペプチダーゼ / X線結晶構造解析 / 体腔形成細菌 / 細菌細胞表層構造 / アルギン酸 / ペプチドグリカン加水分解楮 / スフィンゴモナス属細菌 / 超チャネル / ペプチドグリカン構造 / ペプチドグリカン分解酵素 / X線結晶構造 / フラジェリン進化
Research Abstract

Flagellin, a flagellar protein, in a bacterium Sphingomonas sp. A1 does not participate in the formation of flagella, localizes on the cell surface, and shows highly potent capability to bind alginate, an acidic polysaccharide present in brown sea algae. To elucidate these characteristic properties of flagellin, three-dimensional structures of flagellin, flagellin-anchoring protein on the cell surface, and peptidoglycan-hydrolyzing enzyme were determined and their relationships between structure and function clarified. Based on these results, the biological significance of flagellin were discussed from the standpoint of sensor for alginate, cell surface structure, and molecular evolution.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (42 results)

All 2011 2010 2009 2008

All Journal Article (19 results) (of which Peer Reviewed: 19 results) Presentation (21 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Crystal structure of bacterial cell-surface alginate-binding protein with an M_75 peptidase motif.2011

    • Author(s)
      Yukie Maruyama, Akihito Ochiai, Bunzo Mikami, Wataru Hashimoto, Kousaku Murata
    • Journal Title

      Biochem. Biophys. Res.Commun 405(3)

      Pages: 411-416

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Heterosubunit composition and crystal structures of a novel bacterial M16B metallopeptidase.2011

    • Author(s)
      Yukie Maruyama, Asako Chuma, Bunzo Mikami, Wataru Hashimoto, Kousaku Murata
    • Journal Title

      J.Mol.Biol. 407(1)

      Pages: 180-192

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Heterosubunit composition and crystal structures of a novel bacterial M16B metallopeptidase2011

    • Author(s)
      Yukie Maruyama, Kousaku Murata, et al.
    • Journal Title

      Journal of Molecular Biology

      Volume: 407 Pages: 180-192

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of bacterial cell-surface alginate-binding protein with an M_75 peptidase motif2011

    • Author(s)
      Yukie Maruyama, Kousaku Murata, et al.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 405 Pages: 411-416

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bacterial supersystem for alginate import/metabolism and its environmental and bioenergy applications.2010

    • Author(s)
      Wataru Hashimoto, Shigeyuki Kawai, Kousaku Murata
    • Journal Title

      Bioengineered Bugs. 1(2)

      Pages: 1-13

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mutational studies of the peptidolycan hydrolase FlgJ of Sphingomonas sp.strain A12010

    • Author(s)
      Yukie Maruyama, Kousaku Murata, et al.
    • Journal Title

      Journal of Basic Microbiology

      Volume: 50 Pages: 311-317

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular identification of unsaturated uronate reductase prerequisite for alginate metabolism in Sphingomonas sp.A12010

    • Author(s)
      Ryuichi Takase, Kousaku Murata, et al.
    • Journal Title

      Biochimica et Biophysica Acta

      Volume: 1804 Pages: 1925-1936

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of exotype alginate lyase Atu3025 from Agrobacterium tumefaciens2010

    • Author(s)
      Akihito Ochiai, Kousaku Murata, et al.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 285 Pages: 24519-24528

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] アルギン酸代謝酵素の構造と機能2010

    • Author(s)
      落合秋人, 村田幸作, 他
    • Journal Title

      ビタミン

      Volume: 84 Pages: 525-531

    • NAID

      110007989343

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bacterial supersystem for alginate import/metabolism and its environmental and bioenergy applications2010

    • Author(s)
      Wataru Hashimoto
    • Journal Title

      Bioengineered Bugs 1

      Pages: 1-13

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of the glycosidase family 73 peptidoglycan hydrolase FlgJ.2009

    • Author(s)
      Wataru Hashimoto, Akihito Ochiai, Keiko Momma, Takafumi Itoh, Bunzo Mikami, Yukie Maruyama, Kousaku Murata
    • Journal Title

      Biochem.Biophys.Res.Commun. 381

      Pages: 16-21

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary crystallographic analysis of cell-surface alginate-binding protein Algp7 from Sphingomonas sp. A12009

    • Author(s)
      Wataru Hashimoto
    • Journal Title

      Acta Crystallographica Section F 65

      Pages: 515-517

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of family 14 polysaccharide lyase with pH-dependent modes of action2009

    • Author(s)
      Kohei Ogura
    • Journal Title

      Journal of Biological Chemistry 284

      Pages: 33572-33579

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of the glycosidase family 73 peptidoglycan hydrolase FlgJ2009

    • Author(s)
      Wataru Hashimoto
    • Journal Title

      Biochemical and Biophysical Rresearch Communications 381

      Pages: 16-21

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A putative lipoprotein of Sphingomonas sp. strain Al binds alginate rather than a lipid moiety2009

    • Author(s)
      Jinshan He
    • Journal Title

      FEMS Microbiological Letters 288

      Pages: 221-226

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Superchannel of bacteria : biological significance and new horizons.2008

    • Author(s)
      Kousaku Murata, Shigeyuki Kawai, Bunzo Mikami, Wataru Hashimoto
    • Journal Title

      Biosci. Biotechnol. Biochem. 72(2)

      Pages: 265-277

    • NAID

      10027523626

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of a novel bacterial cell-surface flagellin binding to polysaccharide.2008

    • Author(s)
      Yukie Maruyama, Misato Momma, Bunzo Mikami, Wataru Hashimoto, Kousaku Murata
    • Journal Title

      Biochemstry 47(5)

      Pages: 1393-1402

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Substrate recognition by family 7 alginate lyase from Sphingomonas sp. Al2008

    • Author(s)
      Kohei Ogura
    • Journal Title

      Journal of Molecular Biology 380

      Pages: 373-385

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of a novel bacterial cell-surface flagelllin binding to poiysaccharide2008

    • Author(s)
      Yukie Maruyama
    • Journal Title

      Biochemistry 47

      Pages: 1393-1402

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 体腔形成細菌の分子育種による海洋バイオマスアルギン酸からのエタノール生産2011

    • Author(s)
      竹田浩之, 村田幸作, 他
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Sphingomonas属細菌A1株由来ペプチドグリカン分解酵素の立体構造に基づく触媒機構解析2010

    • Author(s)
      丸山如江、落合秋人、三上文三、橋本渉、村田幸作
    • Organizer
      日本農芸化学会関西支部大会
    • Place of Presentation
      奈良(口頭発表)
    • Year and Date
      2010-10-03
    • Related Report
      2010 Final Research Report
  • [Presentation] Sphingomonas属細菌A1株由来細胞表層に局在するアルギン酸結合タンパク質の遺伝子発現とX線結晶構造解析2010

    • Author(s)
      橋本渉、丸山如江、落合秋人、三上文三、村田幸作
    • Organizer
      日本農芸化学会関西支部大会
    • Place of Presentation
      奈良(口頭発表)
    • Year and Date
      2010-10-03
    • Related Report
      2010 Final Research Report
  • [Presentation] Sphingomoans属細菌A1株の細胞表層に局在するアルギン酸結合タンパク質の遺伝子発現とX線結晶構造解析2010

    • Author(s)
      橋本渉
    • Organizer
      日本農芸化学会関西支部大会
    • Place of Presentation
      近畿大学
    • Year and Date
      2010-10-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] Sphingomonas属細菌A1株由来ペプチドグリカン分解酵素の立体構造に基づく触媒機構解析2010

    • Author(s)
      丸山如江, 村田幸作, 他
    • Organizer
      日本農芸化学会関西支部大会
    • Place of Presentation
      近畿大学
    • Year and Date
      2010-10-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] 細菌由来多糖分解酵素の構造と機能2010

    • Author(s)
      橋本渉
    • Organizer
      関西グライコサイエンスフォーラム
    • Place of Presentation
      大阪市立大学(招待講演)
    • Year and Date
      2010-05-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 細菌由来推定リポタンパク質の脂質結合性評価とその予備的X線結晶構造解析2010

    • Author(s)
      橋本渉
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学 (東京都)
    • Year and Date
      2010-03-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] 窒素固定細菌 Azotobacter vinelandii のシデロフォア生合成遺伝子クラスター2010

    • Author(s)
      米山史紀
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学 (東京都)
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] アルギン酸輸送ABCトラシスポーターの機能解析と予備的X線結晶構造解析2010

    • Author(s)
      伊藤貴文
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学 (東京都)
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] アルギン酸代謝における細菌由来α-ケト酸還元酵素の生理機能と活性中心構造2010

    • Author(s)
      落合秋人
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学 (東京都)
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] 細菌由来メタロペプチダーゼファミリーM16酵素のX線結晶構造2010

    • Author(s)
      丸山如江、橋本渉、村田幸作
    • Organizer
      日本農芸化学会平成22年度大会
    • Place of Presentation
      東京(口頭発表)
    • Year and Date
      2010-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] 細菌由来メタロペプチダーゼファミリーM16酵素のX線結晶構造2010

    • Author(s)
      丸山如江
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学 (東京都)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 細菌におけるATP加水分解エネルギーによる高分子多糖の取り込み2009

    • Author(s)
      西谷悠
    • Organizer
      リン化合物討論会
    • Place of Presentation
      静岡大学 (静岡県)
    • Year and Date
      2009-12-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] Coenzyme-binding mode in α-keto acid reductase responsible for alginate metabolism2009

    • Author(s)
      高瀬隆一
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸ポートアイランド (兵庫県)
    • Year and Date
      2009-10-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Structural determinants responsible for mode of action in polysaccharide lyases for biofuel production2009

    • Author(s)
      落合秋人
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸ポートアイランド (兵庫県)
    • Year and Date
      2009-10-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Expression and function analysis of Sphingomonas alginate ABC transporter2009

    • Author(s)
      伊藤貴文
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸ポートアイランド (兵庫県)
    • Year and Date
      2009-10-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] 多糖の輸送・分解に関わる細菌由来超分子の構造と機能2009

    • Author(s)
      橋本渉
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸ポートアイランド (兵庫県)
    • Year and Date
      2009-10-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] アルギン酸リアーゼvAL-1 (S) は反応pHにより異なる作用様式 (エンド/エキソ型) を示す2009

    • Author(s)
      小倉康平
    • Organizer
      日本生物工学会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] ファミリーGH73ペプチドグリカン分解酵素のX線結晶構造2009

    • Author(s)
      橋本渉、落合秋人、門間敬子、丸山如江、伊藤貴文、三上文三、村田幸作
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡(口頭発表)
    • Year and Date
      2009-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] 細菌によるアルギン酸からのバイオ燃料生産 : エタノール生産菌の分子育種2009

    • Author(s)
      竹田浩之
    • Organizer
      日本農芸化学会年次大会
    • Place of Presentation
      マリンメッセ福岡
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] ファミリーGH73ペプチドグリカン分解酵素のX線結晶構造2009

    • Author(s)
      橋本渉
    • Organizer
      日本農芸化学会年次大会
    • Place of Presentation
      マリンメッセ福岡
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Book] 土壌・地下水汚染の浄化・修復技術(スーパー細菌の創成とダイオキシン分解の高効率化技術)2008

    • Author(s)
      橋本渉
    • Total Pages
      11
    • Publisher
      ブッカーズ(エヌ・ティー・エス(NTS))
    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] 細菌によるアルギン酸からのバイオ燃料生産2009

    • Inventor(s)
      村田幸作
    • Industrial Property Rights Holder
      国立大学法人京都大学・株式会社マルハニチロホールディングス
    • Industrial Property Number
      2009-198972
    • Filing Date
      2009-08-28
    • Related Report
      2009 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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