Development of microbial production system using bioreduction system
| Project/Area Number |
20380051
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kyoto University |
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Principal Investigator |
KATAOKA Michihiko Kyoto University, 生命環境科学研究科, 教授 (90252494)
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| Co-Investigator(Kenkyū-buntansha) |
小川 順 京都大学, 農学研究科, 教授 (70281102)
萩下 大郎 京都大学, 農学研究科, 准教授 (20432346)
清水 昌 京都大学, 農学研究科, 教授 (70093250)
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| Co-Investigator(Renkei-kenkyūsha) |
OGAWA Jun 京都大学, 農学研究科, 教授 (70281102)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2008: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | バイオ還元 / 不斉還元 / 補酵素再生 / 旧黄色酵素 / モノオキシゲナーゼ / ラクトン |
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| Research Abstract |
Bioreduction system using an E. coli transformant co-expressing NAD(P)H-dependent enzyme and cofactor regeneration enzyme genes was applied to the production of other useful compounds. The reactions requiring NAD(P)H as a cofactor, such as C=C bond reductions and monooxigenation were exammined.
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Report
(4 results)
Research Products
(71 results)
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[Journal Article] Expression, purification, crystallization and preliminary X-ray analysis of conjugated polyketone reductase C2 (CPR-C2) from Candida parapsilosis IFO 0708.2009
Author(s)
A. Yamamura, S. Maruoka, J. Ohtsuka, T.Miyakawa, K. Nagata, M.Kataoka, N. Kitamura, S.Shimizu, M.Tanokura
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Journal Title
Acta Crystal. F65
Pages: 1145-1148
Related Report
Peer Reviewed
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[Journal Article] Expression, purification, crystallization and preliminary X-ray analysis of conjugated polyketone reductase C2 (CPR-C2) from Candida parapsilosis IFO 07082009
Author(s)
Yamamura A, Maruoka S, Ohtsuka J, Miyakawa T, Nagata K, Kataoka M, Kitamura N, Shimizu S, Tanokura M.
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Journal Title
Acta Crystallogr.Sect.F Struct.Biol.Cryst Common 65
Pages: 1145-1148
Related Report
Peer Reviewed
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