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Use of the enzymes in the microbial and plant "aldoxime-nitrile pathway"for chiral synthesis

Research Project

Project/Area Number 20380053
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionToyama Prefectural University

Principal Investigator

ASANO Yasuhisa  Toyama Prefectural University, 工学部・生物工学科, 教授 (00222589)

Co-Investigator(Kenkyū-buntansha) KOMEDA Hidenobu  富山県立大学, 工学部, 准教授 (50285160)
FUHSHUKU Ken-ichi  富山県立大学, 工学部, 助教 (70392090)
YAMANE Takashi  名古屋大学, 工学研究科, 教授 (80030055)
YURIMOTO Hiroya  京都大学, 大学院・農学研究科応用生命科学専攻 (00283648)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2009: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2008: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords酵素 / 応用微生物 / 植物 / 遺伝子 / 有機工業化学
Research Abstract

(1) Development of hydroxynitrile lyase (HNL) and its use in optically active cyanohydrin : To construct a library of HNLs, several HNL genes were expressed in heterogonous hosts. We succeeded in cDNA cloning, primary structure elucidation and expression of cDNA of P. mume (Plum) HNL in methanol yeast Pichia pastoris. Full-length cDNA and genomic DNA of a novel S-HNL from a plant, Baliospermum montanum were cloned and sequenced. The genomic DNA contained two introns and one ORF encoding a 263-residue protein (subunit : 29.5 kDa). C-terminal short truncations proved to be successful to access higher productivity in culture medium of E. coli up to 3-fold. The hnl gene was expressed in E. coli and the enzyme was characterized including kinetic studies of 20 substrates for (S)-cyanohydrin synthesis. An R-HNL from Arabidopsis thaliana accepts nitromethane (MeNO2) as a donor in a reaction with aromatic aldehydes to yield (R)-b-nitro alcohols (Henry reaction). This is the first example of the … More R-HNL-catalyzed synthesis of (R)-b-nitro alcohols. (R)-Mandelonitrile was successfully synthesized by an enzymatic transcyanation reaction of benzaldehyde and acetone cyanohydrin catalyzed by a HNL from Eriobotrya japonica (EjHNL) in an aqueous-organic biphasic system. An HNL from leaves of Passiflora edulis (PeHNL) was purified and characterized for the first time. Asymmetric synthesis of (R)-mandelonitrile in a biphasic system employing PeHNL as carried out.
(2) Mechanism of functional expression of HNL from Manihot esculenta : Functional expression of HNL from M. esculenta, MeHNL, was achieved in E. coli. The highly soluble mutant MeHNL-His103Leu were expressed in the systems such as Pichia pastoris, Leishmania tarentolae and two cell-free translation systems, such as E. coli PURE system and Wheat germ translation system. The phenomenon of the functional expression was proven to be specific to the E. coli system.
(3) Dynamic kinetic resolution of amino acid amide : The first crystal structures of a fold-type I racemase are solved for the native form and e-caprolactam-complexed form of a-amino-e-caprolactam racemase. Mutation was successfully done to affect the substrate specificity of the enzyme. Further screening for new amino acid amide racemase was carried out. Less

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (115 results)

All 2011 2010 2009 2008 Other

All Journal Article (26 results) (of which Peer Reviewed: 9 results) Presentation (57 results) Book (22 results) Remarks (4 results) Patent(Industrial Property Rights) (6 results)

  • [Journal Article] Comparative expression of wild-type and highly soluble mutant His103Leu of hydroxynitrile lyase from Manihot esculenta in prokaryotic and eukaryotic expression systems2011

    • Author(s)
      M. Dadashipour, Y. Fukuta, Y. Asano
    • Journal Title

      Protein Expr.Purif. 77(1)

      Pages: 92-97

    • Related Report
      2010 Final Research Report
  • [Journal Article] Characterization of a new (R)-hydroxynitrile lyase from Japanese apricot Prunus mume and cDNA cloning and secretory expression of one of the isozymes in Pichia pastoris.2011

    • Author(s)
      Y. Fukuta, S. Nanda, Y. Kato, H. Yurimoto, Y. Sakai, H. Komeda, Y. Asano
    • Journal Title

      Biosci.Biotech.Biochem. 75(2)

      Pages: 214-220

    • NAID

      10027897395

    • Related Report
      2010 Final Research Report
  • [Journal Article] Comparative expression of wild-type and highly soluble mutant His103Leu of hydroxynitrile lyase from Manihot esculenta in prokaryotic and eukaryotic expression systems2011

    • Author(s)
      M.Dadashipour, et al.
    • Journal Title

      Protein Expr.Purif.

      Volume: 77(1) Pages: 92-97

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of a new (R)-hydroxynitrile lyase from Japanese apricot Prunus mume and cDNA cloning and secretory expression of one of the isozymes in Pichia pastoris2011

    • Author(s)
      Y.Fukuta, et al.
    • Journal Title

      Biosci.Biotech.Biochem.

      Volume: 75(2) Pages: 214-220

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new aryl acylamidase from Rhodococcus sp. strain Oct1 acting on ω-lactams : its characterization and gene expression in Escherichia coli.2010

    • Author(s)
      Y. Fukuta, S. Koizumi, H. Komeda, Y. Asano
    • Journal Title

      Enzyme Microb.Technol. 46(3-4)

      Pages: 237-24

    • Related Report
      2010 Final Research Report
  • [Journal Article] Hydroxynitrile lyase from Passiflora edulis : characteristics and application in asymmetric synthesis of (R)-mandelonitrile.2010

    • Author(s)
      T. Ueatrongchit, K. Tamura, T. Ohmiya, A. H-Kittikun, Y. Asano
    • Journal Title

      Enzyme Microb.Technol. 46(6)

      Pages: 456-465

    • Related Report
      2010 Final Research Report
  • [Journal Article] Parameters influencing on enantioselective transcyanation of (R)-mandelonitrile synthesis by a novel (E)-hydroxynitrile lyase from Eriobotrya japonica2010

    • Author(s)
      T.Ueatrongchit, et al.
    • Journal Title

      Enzyme and Microbial Technology

      Volume: 46(6) Pages: 456-465

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2010

    • Author(s)
      Yasuhisa Asano
    • Journal Title

      Practical Methods in Biocatalysis and Biotransformations, Catalysts for the Fine Chemical Industry(John Whittall and Peter Sutton(eds.))

      Pages: 269-272

    • Related Report
      2009 Annual Research Report
  • [Journal Article] The novel structure of a pyridoxal-5'-phosphate-dependent fold-type I racemase, α-amino-ε-capr olactam racemase from Achromobacter obae.2009

    • Author(s)
      S. Okazaki, A. Suzuki, T. Mizushima, T. Kawano, H. Komeda, Y. Asano, T. Yamane
    • Journal Title

      Biochemistry 48(5)

      Pages: 941-950

    • Related Report
      2010 Final Research Report
  • [Journal Article] Screening, purification, and identification of the enzyme producing N-(L-α-L-aspartyl)-L-phenylalanine methyl ester from L-isoasparagine and L-phenylalanine methyl ester.2009

    • Author(s)
      I. Kira, Y. Asano, K. Yokozeki
    • Journal Title

      J.Biosci.Bioeng. 108(3)

      Pages: 190-193

    • NAID

      110007386280

    • Related Report
      2010 Final Research Report
  • [Journal Article] X-ray crystal structure of the Michaelis complex of aldoxime dehydratase.2009

    • Author(s)
      H. Sawai, H. Sugimoto, Y. Kato, Y. Asano, Y. Shiro, S. Aono
    • Journal Title

      J.Biol.Chem. 284(46)

      Pages: 32089-32096

    • Related Report
      2010 Final Research Report
  • [Journal Article] Molecular properties of a novel (R)-hydroxynitrile lyase from Eriobotrya japonica (loquat).2009

    • Author(s)
      T. Ueatrongchit, A. Kayo, H. Komeda, Y. Asano, A. H-Kittikun
    • Journal Title

      J.Molec.Catal.B : Enzymatic 56(4)

      Pages: 208-214

    • Related Report
      2010 Final Research Report
  • [Journal Article] High yield synthesis of 12-aminolauric acid by "Enzymatic transcrystallization" of ω-laurolactam using ω-laurolactam hydrolase from Acidovorax sp. T31.2009

    • Author(s)
      Y. Fukuta, H. Komeda, Y. Yoshida, Y. Asano
    • Journal Title

      Biosci.Biotech.Biochem. 73(5)

      Pages: 980-986

    • NAID

      10027540396

    • Related Report
      2010 Final Research Report
  • [Journal Article] Parameters influencing on enantioselective transcyanation of(R)-mandelonitrile synthesis by a novel(R)-hydroxynitrile lyase from Eriobotrya japonica2009

    • Author(s)
      T.Ueatrongchit, et al.
    • Journal Title

      J.Molec.Catal.B : Enzymatic 56(4)

      Pages: 208-214

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The novel structure of a pyridoxal-5'-phosphate-dependent fold-type I racemase, α-amino-ε-caprolactam racemase from Achromobacter obae2009

    • Author(s)
      S.Okazaki, et al.
    • Journal Title

      Biochemistry 48(5)

      Pages: 941-950

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] X-ray crystal structure of the Michaelis complex of aldoxime dehydratase2009

    • Author(s)
      H.Sawai, et al.
    • Journal Title

      J.Biol.Chem. 284(46)

      Pages: 32089-32096

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification and characterization of hydroxynitrile lyase from Eriobotrya japonica.2008

    • Author(s)
      T. Ueatrongchit, A. Kayo, H. Komeda, Y. Asano, A. H-.Kittikun
    • Journal Title

      Biosci.Biotech.Biochem. 72(6)

      Pages: 1513-1522

    • Related Report
      2010 Final Research Report
  • [Journal Article] A novel D-stereoselective amino acid amidase from Brevibacterium iodinum : gene cloning, expression and characterization2008

    • Author(s)
      H. Komeda, Y. Asano.
    • Journal Title

      Enzyme Microb.Technol. 43(3)

      Pages: 276-283

    • Related Report
      2010 Final Research Report
  • [Journal Article] Bordusa. D-Amino acid specific proteases and native all-L-proteins : A convenient combination for semisynthesis.2008

    • Author(s)
      N. Wehofsky, A. Pech, S. Liebscher, S. Schmidt, H. Komeda, Y. Asano, F.
    • Journal Title

      Angew.Chem.Int.Ed.Engl. 47(29)

      Pages: 5456-5460

    • Related Report
      2010 Final Research Report
  • [Journal Article] A new enzymatic synthesis of 12-aminolauric acid-Screening, characterization and the use of ω-laurolactam hydrolase-2008

    • Author(s)
      Y. Asano, Y. Fukuta, Y. Yoshida, H. Komeda
    • Journal Title

      Biosci.Biotech.Biochem. 72(8)

      Pages: 2141-2150

    • Related Report
      2010 Final Research Report
  • [Journal Article] Dedecud catalytic mechanism of D-amino acid amidase from Ochrobactrum anthropi SV32008

    • Author(s)
      S. Okazaki, A. Suzuki, H. Komeda, Y. Asano, T. Yamane
    • Journal Title

      J.Synchrotron Rad 15

      Pages: 250-253

    • Related Report
      2010 Final Research Report
  • [Journal Article] Crystal structures of D-amino acid amidase complexed with L-phenylalanine and with L-phenylalanine amide : Insight into D-stereospecificity of D-amino acid amidase from Ochrobactrum anthropi SV32008

    • Author(s)
      S. Okazaki, A Suzuki, T. Mizushima, H. Komeda, Y. Asano, T. Yamane
    • Journal Title

      Acta Cryst. D64

      Pages: 331-334

    • Related Report
      2010 Final Research Report
  • [Journal Article] Purification and characterization of hydroxynitrile lyase from Eriobotry a japonica.2008

    • Author(s)
      T. Ueatrongchit, et al.
    • Journal Title

      Biosci. Biotech. Biochem. 72(6)

      Pages: 1513-1522

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel D-stereoselective amino acid amidase from Brevibacterium iod inum : gene cloning, expression and characterization2008

    • Author(s)
      H. Komeda, Y. Asano
    • Journal Title

      Enzyme Microb. Technol. 43(3)

      Pages: 276-283

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new enzymatic synthesis of 12-aminolauric acid -Screening, characterization and the use of ω-laurolactam hydrolase-.2008

    • Author(s)
      Y. Asano, et al.
    • Journal Title

      Biosci. Biotech. Biochem. 72(8)

      Pages: 2141-2150

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional expression of a plant hydroxynitrile lyase in Escherichia coli by directed evolution : Creation and characterization of highly in vivo soluble mutants

    • Author(s)
      Y. Asano, M. Yamazaki, M. Dadashipour, N. Doi, H. Komeda
    • Journal Title

      Protein Engineering (accepted)

    • Related Report
      2010 Final Research Report
  • [Presentation] α-アミノ-εカプロラクタム(ACL)ラセマーゼの機能解明を志向したACLアナログの合成2011

    • Author(s)
      冨宿賢一、浅野泰久
    • Organizer
      2011年度農芸化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-26
    • Related Report
      2010 Final Research Report
  • [Presentation] Enzymatic Processes which Replaced Some of Already Established Chemical2011

    • Author(s)
      浅野泰久
    • Organizer
      Processes Symposium on Biomass to Fuels & Chemicals
    • Place of Presentation
      Singapore
    • Year and Date
      2011-01-13
    • Related Report
      2010 Final Research Report
  • [Presentation] α-アミノ-ε-カプロラクタムラセマーゼの基質特異性の改変2010

    • Author(s)
      浅野泰久
    • Organizer
      第422回ビタミンB研究協議会
    • Place of Presentation
      京都
    • Year and Date
      2010-11-27
    • Related Report
      2010 Final Research Report
  • [Presentation] Achromobacter obae由来α-アミノ-ε-カプロラクタムラセマーゼの基質特異性の改変2010

    • Author(s)
      徳南宏祐、浅野泰久
    • Organizer
      北陸合同バイオシンポジウム
    • Place of Presentation
      福井
    • Year and Date
      2010-11-12
    • Related Report
      2010 Final Research Report
  • [Presentation] Mutation of D-aminopeptidase from Ochrobactrum anthropi2010

    • Author(s)
      氷見茉莉子、浅野泰久
    • Organizer
      北陸合同バイオシンポジウム
    • Place of Presentation
      福井
    • Year and Date
      2010-11-12
    • Related Report
      2010 Final Research Report
  • [Presentation] Development of enzymes for industrial and diagnostic uses2010

    • Author(s)
      浅野泰久
    • Organizer
      第11回日中韓酵素工学会議
    • Place of Presentation
      Chengdu, China
    • Year and Date
      2010-11-07
    • Related Report
      2010 Final Research Report
  • [Presentation] Dynamic kinetic resolution of racemic α-aminonitriles to form chiral α-amino acids2010

    • Author(s)
      安川和志, 長谷見隆司, 浅野泰久
    • Organizer
      第11回日中韓酵素工学会議
    • Place of Presentation
      Chengdu, China
    • Year and Date
      2010-11-07
    • Related Report
      2010 Final Research Report
  • [Presentation] Functional expression of a plant enzyme S-hydroxynitrile lyase from Manihot esculenta in Escherichia coli2010

    • Author(s)
      Y.Asano, et al.
    • Organizer
      The 12^<th> Japanese-Swiss Meeting on Biotechnology and Bioprocess Development
    • Place of Presentation
      富山市
    • Year and Date
      2010-10-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] ω-ラウロラクタムの「酵素的結晶変換法」による12-アミノラウリン酸の大量合成2010

    • Author(s)
      福田泰久、米田英伸、浅野泰久
    • Organizer
      第14回生体触媒化学シンポジウム
    • Place of Presentation
      静岡
    • Year and Date
      2010-09-23
    • Related Report
      2010 Final Research Report
  • [Presentation] 非立体選択的ニトリルヒドラターゼを利用したα-アミノニトリルのダイナミックな光学分割2010

    • Author(s)
      安川和志、長谷見隆司、浅野泰久
    • Organizer
      第6回D-アミノ酸研究会学術講演会
    • Place of Presentation
      富山
    • Year and Date
      2010-09-17
    • Related Report
      2010 Final Research Report
  • [Presentation] Asymmetric Henry Reaction Catalyzed by (R)-Hydroxynitrile Lyase from Arabidopsis thaliana2010

    • Author(s)
      K. Fuhshuku, Y.Asano
    • Organizer
      Biocat 2010国際会議
    • Place of Presentation
      Hamburg, Germany
    • Year and Date
      2010-08-30
    • Related Report
      2010 Final Research Report
  • [Presentation] The Role of Lys65 Residue in Catalytic Triad of D-aminopeptidase from Ochrobactrum anthropi Revealed by Bioinformatic Analysis and Molecular Modeling2010

    • Author(s)
      I.G. Khaliullin, D. Suplatov, V. Svedas, Y.Asano, M.Otsuka, D. Shalaeva
    • Organizer
      Biocat2010国際会議
    • Place of Presentation
      Hamburg, Germany
    • Year and Date
      2010-08-30
    • Related Report
      2010 Final Research Report
  • [Presentation] Studies on the plant enzyme hydroxynitrile lyase from Baliospermum montanum and Manihot esculenta: characterization and mechanism of functional expression2010

    • Author(s)
      M. Dadashipour, Y.Asano
    • Organizer
      日本生物工学会2010年度中部支部例会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-08-02
    • Related Report
      2010 Final Research Report
  • [Presentation] Functional Expression of a Plant Enzyme S-Hydroxynitrile lyase from Manihot esculenta in Escherichia coli2010

    • Author(s)
      Y.Asano, M. Dadashipour
    • Organizer
      The 12^<th> Japanese-Swiss Meeting on Biotechnology and Bioprocess Development.
    • Place of Presentation
      富山
    • Related Report
      2010 Final Research Report
  • [Presentation] Functional expression of a plant S-hydroxynitrile lyase from Manihot esculenta in Escherichia coli.2010

    • Author(s)
      M. Dadashipour, Y.Fukuta, Y.Asano
    • Organizer
      The 12^<th> Japanese-Swiss Meeting on Biotechnology and Bioprocess Development.
    • Place of Presentation
      富山
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物酵素ヒドロキシニトリルリアーゼが触媒する光学活性物質の合成2009

    • Author(s)
      冨宿賢一、福田泰久、Samik Nanda、浅野泰久
    • Organizer
      富山・福井・石川県立大学合同シンポジウム
    • Place of Presentation
      福井
    • Year and Date
      2009-12-18
    • Related Report
      2010 Final Research Report
  • [Presentation] 酵素的結晶変換法による12-アミノラウリン酸の大量合成2009

    • Author(s)
      福田泰久、米田英伸、吉田洋一、浅野泰久
    • Organizer
      富山・福井・石川県立大学合同シンポジウム
    • Place of Presentation
      福井
    • Year and Date
      2009-12-18
    • Related Report
      2010 Final Research Report
  • [Presentation] B6酵素α-アミノ-ε-カプロラクタムラセマーゼの構造機能解析と応用2009

    • Author(s)
      浅野泰久、山根隆
    • Organizer
      日本ビタミン学会、ビタミンB委員会
    • Place of Presentation
      京都
    • Year and Date
      2009-11-28
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物酵素ヒドロキシニトリルリアーゼが触媒する光学活性物質合成2009

    • Author(s)
      冨宿賢一、福田泰久、Samik Nanda、浅野泰久
    • Organizer
      酵素工学研究会第62回講演会
    • Place of Presentation
      東京
    • Year and Date
      2009-11-13
    • Related Report
      2010 Final Research Report
  • [Presentation] 酵素的結晶変換による12-アミノラウリン酸の新しい合成法の開発に関する研究2009

    • Author(s)
      福田泰久、小泉真平、吉田洋一、米田英伸、浅野泰久
    • Organizer
      酵素工学研究会第62回講演会
    • Place of Presentation
      東京
    • Year and Date
      2009-11-13
    • Related Report
      2010 Final Research Report
  • [Presentation] Use of penicillin-recognizing enzymes in L-and D-peptides, and D-amino acid syntheses2009

    • Author(s)
      浅野泰久
    • Organizer
      Italy-Japan symposium, new trends in enzyme science and technology
    • Place of Presentation
      Naples, Italy
    • Year and Date
      2009-10-26
    • Related Report
      2010 Final Research Report
  • [Presentation] D立体選択的ペプチダーゼ類のD-アミノ酸およびペプチド合成への利用2009

    • Author(s)
      浅野泰久
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-22
    • Related Report
      2010 Final Research Report
  • [Presentation] Use of penicillin recognizing enzymes in L-peptide and D-amino acid synthesis2009

    • Author(s)
      浅野泰久
    • Organizer
      15^<th> German-Japanese Workshop in Enzyme Technology
    • Place of Presentation
      Rostoc, Germany
    • Year and Date
      2009-09-26
    • Related Report
      2010 Final Research Report
  • [Presentation] Enzymatic Transcrystallization of ω-Laurolactam to 12-Aminolauric acid by ω-laurolactam hydrolase from Acidovorax sp. T312009

    • Author(s)
      浅野泰久
    • Organizer
      Enzyme Engineering XX
    • Place of Presentation
      Groningen, The Netherlands
    • Year and Date
      2009-09-23
    • Related Report
      2010 Final Research Report
  • [Presentation] Expanding the Synthetic Applicability of Hydroxynitrile Lyase-catalyzed Reaction2009

    • Author(s)
      K. Fuhshuku, Y.Fukuta, S. Nanda, Y.Asano
    • Organizer
      Enzyme Engineering XX
    • Place of Presentation
      Groningen, The Netherlands
    • Year and Date
      2009-09-23
    • Related Report
      2010 Final Research Report
  • [Presentation] Asymmetric transcyanation synthesis of (R)-mandelonitrile by using a novel (R)-hydroxynitrile lyase from Passiflora edulis2009

    • Author(s)
      T. Ueatrongchit, K. Tamura, H.Komeda, Y.Asano, A. H-Kittikun
    • Organizer
      Biotrans 2009
    • Place of Presentation
      Berne, Switzerland
    • Year and Date
      2009-07-09
    • Related Report
      2010 Final Research Report
  • [Presentation] Dynamic kinetic resolution of racemic α-aminonitriles to form chiral amino acids2009

    • Author(s)
      安川和志, 長谷見隆司, 米田英伸, 浅野泰久
    • Organizer
      Biotrans 2009
    • Place of Presentation
      Berne, Switzerland
    • Year and Date
      2009-07-09
    • Related Report
      2010 Final Research Report
  • [Presentation] Dynamic kinetic resolution of α-aminonitriles and amino acid amides2009

    • Author(s)
      Y.Asano, K.Yasukawa, S.Maruyama, R.Hasemi, S.Okazaki, T.Yamane, S.Yamaguchi, H.Komeda
    • Organizer
      Biotrans 2009
    • Place of Presentation
      Berne, Switzerland
    • Year and Date
      2009-07-07
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] Dynamic kinetic resolution of α-aminonitriles and amino acid amides2009

    • Author(s)
      Y.Asano, K.Yasukawa, S.Maruyama, R.Hasemi, S.Okazaki, T.Yamane, S. Yamaguchi, H.Komeda
    • Organizer
      The 1^<st> International Confererence of D-amino acid Research
    • Place of Presentation
      淡路島
    • Year and Date
      2009-07-01
    • Related Report
      2010 Final Research Report
  • [Presentation] ウメ(Prunus mume)由来(R)-hydroxynitrilelyaseの遺伝子解析2009

    • Author(s)
      福田泰久、米田英伸、浅野泰久
    • Organizer
      2009年農芸化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] Characterization of a novel(S)-Hydroxynitrile lyase from plant Baliospermum montanum expressed in E.coli2009

    • Author(s)
      Mohammad DADASHIPOUR、金瀬由梨奈、冨宿賢一、米田英伸、浅野泰久
    • Organizer
      2009年農芸化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] ウメ(Prunus mume)由来(R)-hydroxynitrilelyaseのPichia pastorisによる分泌発現2009

    • Author(s)
      福田泰久、米田英伸、浅野泰久
    • Organizer
      2010 年度農芸化学会大会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] Functional expression of cassava plant enzyme (S)-hydroxynitrile lyase in E.coli-Comparative studies on the selected mutants created by directed-evolution2009

    • Author(s)
      M. Dadashipour、M. Yamazaki、H.Komeda, Y.Asano
    • Organizer
      2010 年度農芸化学会大会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] ヒドロキシニトリルリアーゼが触媒する光学活性なβ-ニトロアルコールの合成2009

    • Author(s)
      冨宿賢一、浅野泰久
    • Organizer
      2010年度農芸化学会大会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] ω-オクタラクタム加水分解酵素の精製と酵素化学的諸性質2009

    • Author(s)
      小泉真平、福田泰久、米田英伸、浅野泰久
    • Organizer
      2009年農芸化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] ニトリルヒドラターゼの大腸菌内での発現と光学活性アミノ酸合成への利用2009

    • Author(s)
      安川和志、長谷見隆司、米田英伸、浅野泰久
    • Organizer
      2009年農芸化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] A novel(R)-hydroxynitrile lyase from Passiflora edulis and asymmetric transcyanation synthesis of(R)-mandelonitrile2009

    • Author(s)
      Techawaree UEATRONGCHIT, Kenichirou TAMURA, Hidenobu KOMEDA, Yasuhisa ASANO, Aran H-KITTIKUN
    • Organizer
      2009年農芸化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] ケトキシムの転位反応を触媒する微生物の探索2009

    • Author(s)
      冨宿賢一、浅野泰久
    • Organizer
      2009年農芸化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] D-アミノペプチダーゼの発見とD-アミノ酸合成への応用2009

    • Author(s)
      浅野泰久
    • Organizer
      2009年農芸化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] Achromobacter obae由来アミノ酸アミドラセマーゼの基質特異性の改変2009

    • Author(s)
      丸山沙都子、大塚稔、福田泰久、徳南宏祐、岡崎誠司、山根隆、米田英伸、浅野泰久
    • Organizer
      2010年度農芸化学会大会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] Enzymatic racemisation of amino acid amides : Screening, purification and characterization of racemase from Ensifer adhaerens2009

    • Author(s)
      P. KAUL、M.OTSUKA、Y.ASANO
    • Organizer
      2010年度農芸化学会大会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] 新しい酵素反応の開発と産業利用に関する研究2008

    • Author(s)
      浅野泰久
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-11
    • Related Report
      2010 Final Research Report
  • [Presentation] D-立体選択的ペプチダーゼ類の機能解析と応用2008

    • Author(s)
      米田英伸、浅野泰久
    • Organizer
      石川県立大学・富山県立大学合同シンポジウム
    • Place of Presentation
      氷見
    • Year and Date
      2008-11-29
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物酵素ヒドロキシニトリルリアーゼが触媒する有機合成2008

    • Author(s)
      浅野泰久、福田泰久、S. Nanda、冨宿賢一
    • Organizer
      石川県立大学・富山県立大学合同シンポジウム
    • Place of Presentation
      氷見
    • Year and Date
      2008-11-29
    • Related Report
      2010 Final Research Report
  • [Presentation] ラセミ体アミノニトリルから光学活性アミノ酸へのダイナミックな光学分割2008

    • Author(s)
      安川和志、長谷見隆司、米田英伸、浅野泰久
    • Organizer
      石川県立大学・富山県立大学合同シンポジウム
    • Place of Presentation
      氷見
    • Year and Date
      2008-11-29
    • Related Report
      2010 Final Research Report
  • [Presentation] 新しい酵素機能の開拓と産業利用に関する研究2008

    • Author(s)
      浅野泰久
    • Organizer
      京都大学大学院農学研究科発酵生理学特別セミナー「バイオ戦略と研究開発」
    • Place of Presentation
      京都
    • Year and Date
      2008-11-27
    • Related Report
      2010 Final Research Report
  • [Presentation] 有用酵素の新機能開拓と産業利用2008

    • Author(s)
      浅野泰久
    • Organizer
      酵素工学研究会30周年記念シンポジウム
    • Place of Presentation
      木更津市
    • Year and Date
      2008-11-13
    • Related Report
      2010 Final Research Report
  • [Presentation] Functional analysis and application of D-stereoselective peptidases and amidases from microorganisms2008

    • Author(s)
      米田英伸, 浅野泰久
    • Organizer
      The tenth Korea-China-Japan Joint Symposium on Enzyme Engineering
    • Place of Presentation
      Busan, Korea
    • Year and Date
      2008-11-04
    • Related Report
      2010 Final Research Report
  • [Presentation] The novel structure and function of pyridoxal-5'-phosphate-dependent Fold type I racemase, α-amino-ε-caprolactam racemase from Achromobacter obae2008

    • Author(s)
      岡崎誠司, 鈴木淳巨, 米田英伸, 浅野泰久, 山根隆
    • Organizer
      The tenth Korea-China-Japan Joint Symposium on Enzyme Engineering
    • Place of Presentation
      Busan, Korea
    • Year and Date
      2008-11-04
    • Related Report
      2010 Final Research Report
  • [Presentation] 新しい酵素反応の開発と産業利用に関する研究(バイオインダストリー協会賞受賞講演)2008

    • Author(s)
      浅野泰久
    • Organizer
      Bio Japan 2008
    • Place of Presentation
      横浜市
    • Year and Date
      2008-10-15
    • Related Report
      2010 Final Research Report
  • [Presentation] Brevibacteriumiodinum由来の新規D-アミノ酸アミダーゼの遺伝子クローニング、酵素精製及び諸性質の解明2008

    • Author(s)
      米田英伸、浅野泰久
    • Organizer
      第4回D-アミノ酸研究会学術講演会
    • Place of Presentation
      名古屋市
    • Year and Date
      2008-09-20
    • Related Report
      2010 Final Research Report
  • [Presentation] α-アミノ-ε-カプロラクタムラセマーゼの立体構造と、推定上の基質認識機構2008

    • Author(s)
      岡崎誠司、鈴木淳巨、米田英伸、浅野泰久、山根隆
    • Organizer
      第4回D-アミノ酸研究会学術講演会
    • Place of Presentation
      名古屋市
    • Year and Date
      2008-09-19
    • Related Report
      2010 Final Research Report
  • [Presentation] Enzymatic processes which replaced some of already established chemical processes2008

    • Author(s)
      浅野泰久
    • Organizer
      BioKorea 2008
    • Place of Presentation
      Osong, Korea
    • Year and Date
      2008-09-09
    • Related Report
      2010 Final Research Report
  • [Presentation] Cloning and expression of a novel (S)-hydroxynitrile lyase from plant Baliospermum montanum in Escherichia coli2008

    • Author(s)
      M. Dadashipour, M. Yamazaki, K. Momonoi, K. Tamura, H.Komeda, Y.Asano
    • Organizer
      4^<th> International Congress on Biocatalysis
    • Place of Presentation
      Hamburg,Germany
    • Year and Date
      2008-09-03
    • Related Report
      2010 Final Research Report
  • [Presentation] A new enzymatic synthesis of 12-aminolauric acid from ω-laurolactam using omega-laurolactam hydrolase2008

    • Author(s)
      福田泰久, 米田英伸, 吉田洋一, 浅野泰久
    • Organizer
      4^<th> International Congress on Biocatalysis
    • Place of Presentation
      Hamburg, Germany
    • Year and Date
      2008-09-01
    • Related Report
      2010 Final Research Report
  • [Presentation] Acidovorax sp. T31由来omega-ラウロラクタム加水分解酵素を用いた12-アミノラウリン酸の酵素的合成2008

    • Author(s)
      福田泰久、米田英伸、吉田洋一、浅野泰久
    • Organizer
      第60回日本生物工学会大会(2008年)
    • Place of Presentation
      仙台市
    • Year and Date
      2008-08-27
    • Related Report
      2010 Final Research Report
  • [Presentation] Dynamic kinetic resolution of aminonitrile and amino acid amide to form chiral amino acids2008

    • Author(s)
      浅野泰久
    • Organizer
      Gordon Conferences on Biocatalysis
    • Place of Presentation
      Rhode Island, USA
    • Year and Date
      2008-07-07
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Book] In Practical Methods in Biocatalysis and Biotransformations(A new (R)-hydroxynitrile lyase from Prunus mume for the asymmetric synthesis of cyanohydrins)(John Whittall and Peter Sutton (eds.))2010

    • Author(s)
      浅野泰久
    • Publisher
      Catalysts for the Fine Chemical Industry
    • Related Report
      2010 Final Research Report
  • [Book] Tools for Enzyme Discovery-Industrial enzymes, biocatalysis and enzyme evolution-Manual of Industrial Microbiology and Biotechnology(3rd Edition)(Ed.: R.H. Baltz, J.E. Davies, and A. Demain)2010

    • Author(s)
      浅野泰久
    • Publisher
      American Society for Microbiology
    • Related Report
      2010 Final Research Report
  • [Book] D-アミノ酸の酵素的合成、酵素利用技術大系2010

    • Author(s)
      浅野泰久
    • Publisher
      NTS出版
    • Related Report
      2010 Final Research Report
  • [Book] 生物学辞典(「アクリルアミド」、「イノシン酸」「グアニル酸」)2010

    • Author(s)
      浅野泰久
    • Publisher
      東京化学同人
    • Related Report
      2010 Final Research Report
  • [Book] "Tools for Enzyme Discovery" In "Manual of Industrial Microbiology and Biotechnology" (3rd Edition)2010

    • Author(s)
      Y.Asano(分担執筆)
    • Total Pages
      766
    • Publisher
      American Society for Microbiology
    • Related Report
      2010 Annual Research Report
  • [Book] 酵素法によるD-アミノ酸の製造、微生物によるものづくり-化学法に代わるホワイトバイオテクノロジーの全て-2008

    • Author(s)
      浅野泰久
    • Publisher
      CMC出版
    • Related Report
      2010 Final Research Report
  • [Book] 酵素ハンドブックMandelonitrile lyase2008

    • Author(s)
      浅野泰久
    • Total Pages
      774
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Book] 酵素ハンドブックHydroxynitrile lyase2008

    • Author(s)
      浅野泰久
    • Total Pages
      774
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Book] 酵素ハンドブックMaleate hydratase2008

    • Author(s)
      浅野泰久
    • Total Pages
      792
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Book] 酵素ハンドブックL(+)-Tartrate dehydratase2008

    • Author(s)
      浅野泰久
    • Total Pages
      793
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Book] 酵素ハンドブック3-Isopropylmalate dehydratase2008

    • Author(s)
      浅野泰久
    • Total Pages
      793
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Book] 酵素ハンドブック(S)-2-Methylmalate dehydratase2008

    • Author(s)
      浅野泰久
    • Total Pages
      793
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Book] 酵素ハンドブック(R)-2-Methylmalate dehydratase2008

    • Author(s)
      浅野泰久
    • Total Pages
      794
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Book] 酵素ハンドブック3-Cyanoalanine hydratase2008

    • Author(s)
      浅野泰久
    • Total Pages
      799
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Book] 酵素ハンドブックMethylitaconate Δ-isomerase2008

    • Author(s)
      浅野泰久
    • Total Pages
      852
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Book] 酵素ハンドブックCyanide hydratase2008

    • Author(s)
      浅野泰久、米田英伸
    • Total Pages
      799
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Book] 酵素ハンドブックCyanamide hydratase2008

    • Author(s)
      浅野泰久、米田英伸
    • Total Pages
      800
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Book] 酵素ハンドブックPseudouridylate synthase2008

    • Author(s)
      浅野泰久、米田英伸
    • Total Pages
      800
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Book] 酵素ハンドブックProtoaphilin-aglucone dehydratase(cyclizing)2008

    • Author(s)
      浅野泰久、米田英伸
    • Total Pages
      800
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Book] 酵素ハンドブックDimethylmalate hydratase2008

    • Author(s)
      浅野泰久、米田英伸
    • Total Pages
      803
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Book] 酵素ハンドブックMethylaspartate ammonia-lyase2008

    • Author(s)
      浅野泰久、加藤康夫
    • Total Pages
      817
    • Publisher
      朝倉書店
    • Related Report
      2010 Final Research Report
  • [Book] 微生物によるものづくり-化学法に代わるホワイトバイオテクノロジーの全て-(分担執筆)2008

    • Author(s)
      浅野泰久
    • Publisher
      CMC出版
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.pu-toyama.ac.jp/BR/asano/homepage.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.pu-toyama.ac.jp/BR/asano/homepage.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.pu-toyama.ac.jp/BR/asano/homepage.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.pu-toyama.ac.jp/BR/asano/homepage.html

    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] L体アミノ酸アミド不斉加水分解酵素及びそれをコードするDNA2011

    • Inventor(s)
      浅野泰久、井上敦
    • Acquisition Date
      2011-03-16
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] (R)-ヒドロキシニトリルリアーゼ及びその利用方法2009

    • Inventor(s)
      浅野泰久
    • Acquisition Date
      2009-05-20
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] (R)-体のアミド結合を選択的に加水分解するアミダーゼ遺伝子及びその利用2009

    • Inventor(s)
      阪本剛、上田誠、浅野泰久
    • Acquisition Date
      2009-06-10
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] L-ペニシラミンの製造方法2009

    • Inventor(s)
      浅野泰久、田村豊、田中昭宣
    • Acquisition Date
      2009-07-15
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] (S)-ヒドロキシニトリルリアーゼ2009

    • Inventor(s)
      浅野泰久
    • Acquisition Date
      2009-11-11
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)]2008

    • Inventor(s)
      浅野泰久
    • Acquisition Date
      2008-07-09
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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