Molecular Mechanism of trans-translation

• Project/Area Number: 20380057
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Hirosaki University
• Principal Investigator: HIMENO Hyouta Hirosaki University, 農学生命科学部, 教授 (80208785)
• Co-Investigator(Kenkyū-buntansha): MUTO Akira 弘前大学, 農学生命科学部, 研究員 (80034635)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: 遺伝子発現 / tmRNA / trans-translation / ribosome / molecular mimicry / SmpB / translation / EF-Tu

Research Abstract

Trans-translation is an irregular translation system, in which tmRNA (transfer and messenger RNA) rescues the ribosome stalled at the 3' end of truncated mRNA lacking a termination codon to produce a chimeric polypeptide from two messages on truncated mRNA and tmRNA. It provides an incomplete nascent polypeptide from a truncated mRNA with a tag for degradation to promote degradation of truncated mRNA. This project revealed the molecular mechanism of the early stage of trans-translation based on our proposal that SmpB, a tmRNA binding protein, mimics both tRNA and mRNA.

Research Products

• [Journal Article] tRNA/mRNA mimicry by tmRNA and SmpB in trans-translation. (2011)
  • Author(s): Kurita D., Muto A., Himeno H.
  • Journal Title: Journal of Nucleic Acids 2011
• [Journal Article] RsgA releases RbfA from 30S ribosomal subunit during a late stage of ribosome biosynthesis. (2011)
  • Author(s): Goto S., Kato S., Kimura T., Muto A., Himeno H.
  • Journal Title: EMBO Journal 30 Pages: 104-114
• [Journal Article] tRNA/mRNA mimicry by tmRNA and SmpB in traps-translation (2011)
  • Author(s): Kurita, D., Muto, A., Himeno, H.
  • Journal Title: J.Nucleic Acids Volume: 2011
  • Peer Reviewed
• [Journal Article] RsgA releases RbfA from 30S ribosomal subunit during a late stage of ribosome biosynthesis (2011)
  • Author(s): Goto, S., Kato, S., Kimura, T., Muto, A., Himeno, H.
  • Journal Title: EMBO J. Volume: 30 Pages: 104-114
  • Peer Reviewed
• [Journal Article] Role of the C-terminal tail of SmpB in the early stage of trans-translation. (2010)
  • Author(s): Kurita D., Muto A., Himeno H.
  • Journal Title: RNA 16 Pages: 980-990
• [Journal Article] A small nucleolar RNA functions in rRNA processing in C. elegans. (2010)
  • Author(s): Hokii Y., Sasano Y., Sato M., Sakamoto H., Sakata K., Shingai R., Taneda A., Oka S., Himeno H. Muto A., Fujiwara T., Ushida C.
  • Journal Title: Nucleic Acids Research 38 Pages: 5909-5918
• [Journal Article] Novel factor rescues ribosomes trapped on non-stop mRNAs. (2010)
  • Author(s): 姫野俵太
  • Journal Title: Molecular Microbiology 78 Pages: 789-791
• [Journal Article] Role of the C-terminal tail of SmpB in the early stage of trans-transl ation (2010)
  • Author(s): Kurita, D., Muto, A., Himeno, H.
  • Journal Title: RNA Volume: 116 Pages: 980-990
  • Peer Reviewed
• [Journal Article] A small nucleolar RNA functions in rRNA processing in C.elegans (2010)
  • Author(s): Hokii, Y., Sasano, Y., Sato, M., Sakamoto, H., Sakata, K., Shingai, R., Taneda, A., Oka, S., Himeno, H., Muto, A., Fujiwara, T., Ushida, C.
  • Journal Title: Nucleic Acids Res. Volume: 38 Pages: 5909-5918
  • Peer Reviewed
• [Journal Article] Novel factor rescues ribosomes trapped on non-stop mRNAs (2010)
  • Author(s): Himeno, H.
  • Journal Title: Mol.Microbiol. Volume: 78 Pages: 789-791
  • Peer Reviewed
• [Journal Article] Role of the C-terminal tail of SmpB in the early stage of trans-translation. (2010)
  • Author(s): Kurita, D., Muto, A., Himeno, H.
  • Journal Title: RNA (印刷中)
  • Peer Reviewed
• [Journal Article] Trans-translation by tmRNA and SmpB. (2009)
  • Author(s): Une M., Kurita D., Muto A., Himeno H.
  • Journal Title: Nucleic Acids Symposium Series 53 Pages: 305-306
• [Journal Article] Physiological role of RsgA in ribosome biogenesis. (2009)
  • Author(s): Hase Y., Yokoyama S., Kimura T., Goto S., Muto A., Himeno H.
  • Journal Title: Nucleic Acids Symposium Series 53 Pages: 307-308
• [Journal Article] Trans-translation is involved in the CcpA-dependent tagging and degradation of TreP in Bacillus subtilis. (2009)
  • Author(s): Ujiie H., Matsutani T., Tomatsu H., Fujihara A., Ushida C., Miwa Y., Fujita Y., Himeno H. Muto A.
  • Journal Title: Journal of Biochemistry 145 Pages: 59-66
  • NAID: 10024914415
• [Journal Article] Removal of a ribosome small subunit-dependent GTPase confers salt-resistance on Escherichia coli cells. (2009)
  • Author(s): Hase Y., Yokoyama S., Muto A., Himeno H.
  • Journal Title: RNA 15 Pages: 1766-1774
• [Journal Article] 2つのtRNA/mRNAハイブリッド-トランス・トランスレーション- (2009)
  • Author(s): 姫野俵太、栗田大輔、武藤あきら
  • Journal Title: 蛋白質核酸酵素増刊 54(16) Pages: 2201-2206
• [Journal Article] Removal of a ribosome small subunit-dependent GTPase confers salt-resistance on Escherichia coli cells. (2009)
  • Author(s): Hase, Y., Yokoyama, S., Muto, A., Himeno, H.
  • Journal Title: RNA 15 Pages: 1766-1774
  • Peer Reviewed
• [Journal Article] Trans-translation by tmRNA and SmpB. (2009)
  • Author(s): Une, M., Kurita, D., Muto, A., Himeno, H.
  • Journal Title: Nucleic Acids Symp.Ser. 53 Pages: 305-306
• [Journal Article] Physiological role of RsgA in ribosome biogenesis. (2009)
  • Author(s): Hase, Y., Yokoyama, S., Kimura, T., Goto, S., Muto, A., Himeno, H.
  • Journal Title: Nucleic Acids Symp.Ser. 53 Pages: 307-308
• [Journal Article] 2つのtRNA/mRNAハイブリッド-トランス・トランスレーション- (2009)
  • Author(s): 姫野俵太、栗田大輔、武藤あきら
  • Journal Title: 蛋白質核酸酵素(増刊:mRNAプログラム 多様性と非対称性の獲得戦略) 54 Pages: 2201-2206
• [Journal Article] Trans-translation is involved in the CcpA-dependent tagging and degra dation of TreP in Bacillus subtilis (2009)
  • Author(s): Ujiie, H.
  • Journal Title: J. Biochem 145 Pages: 59-66
  • Peer Reviewed
• [Journal Article] トランストランスレーション (2008)
  • Author(s): 姫野俵太、武藤あきら
  • Journal Title: 蛋白質核酸酵素 53(8) Pages: 975-975
• [Journal Article] mRNAを擬態するタンパク質 (2008)
  • Author(s): 栗田大輔、武藤あきら、姫野俵太
  • Journal Title: 化学と生物 46(7) Pages: 465-471
  • NAID: 10024333950
• [Journal Article] Ribosome-small-subunit-dependent GTPase interacts with the tRNA binding sites on the ribosome. (2008)
  • Author(s): Kimura T., Takagi K., Hirata Y., Hase Y., Muto A., Himeno H.
  • Journal Title: Journal of Molecular Biology 381 Pages: 467-477
• [Journal Article] tmRNA-dependent trans-translation is required for sporulation in Bacillus subtilis. (2008)
  • Author(s): Abe T., Sakaki K., Fujihara A., Ujiie H., Ushida C., Himeno H., Sato T., Muto A.
  • Journal Title: Molecular Microbiology 69 Pages: 1491-1498
• [Journal Article] Ribosome-small-subunit-dependent GTPase interacts with the tRNA bin ding sites on the ribosome (2008)
  • Author(s): Kimura, T.
  • Journal Title: J. Mol. Biol 381 Pages: 467-477
  • Peer Reviewed
• [Journal Article] tmRNA-dependent trans-translation is required for sporulation in Bacillus subtilis (2008)
  • Author(s): Abe, T.
  • Journal Title: Mol. Microbiol 69 Pages: 1491-1498
  • Peer Reviewed
• [Journal Article] トランストランスレーション (2008)
  • Author(s): 姫野俵太
  • Journal Title: キーワード : 蛋白質の一生(蛋白質核酸酵素増刊) 53 Pages: 975-975
• [Journal Article] mRNAを擬態ずるタンパク質 (2008)
  • Author(s): 栗田大輔
  • Journal Title: 化学と生物 47 Pages: 465-471
  • Peer Reviewed
• [Presentation] Role of the C-terminal tail of SmpB during trans-translation. (2010)
  • Author(s): Kurita D., Une M., Muto A., Himeno H.
  • Organizer: 23^<rd> tRNA Workshop.
  • Place of Presentation: Aveiro, Portugal
• [Presentation] Molecular mimicry of tRNA/mRNA during trans-translation. (2010)
  • Author(s): Kurita D., Une M., Muto A., Himeno H.
  • Organizer: ASBMB 2010 Annual Meeting
  • Place of Presentation: Anaheim, USA
• [Presentation] Structural studies for RsgA/YjeQ, a ribosome maturation factor. (2010)
  • Author(s): Takemoto C., Connell S.R., Hase, Y., Naoe C., Wang H., Kaminishi T., Kikuchi T., Hirata Y., Kurita D., Muto A., Muto Y., Yokoyama S., Himeno H.
  • Organizer: Ribosomes 2010 Meeting
  • Place of Presentation: Orvieto, Italy
• [Presentation] Molecular mimicry by tmRNA/SmpB during trans-translation. (2010)
  • Author(s): Kurita D., Une M., Muto A., Himeno H.
  • Organizer: Ribosomes 2010 Meeting
  • Place of Presentation: Orvieto, Italy
• [Presentation] The mutation of the C. elegans genomic locus encoding a snoRNA has a defect in the pre-rRNA processing. (2010)
  • Author(s): Ushida C., Hokii, Y., Sasano Y., Sato M., Sakamoto H., Himeno H. Muto A., Fujiwara T.
  • Organizer: RNA2010, Fifteenth Annual Meeting of the RNA Society
  • Place of Presentation: Seattle
• [Presentation] Trans-translation by tmRNA/SmpB. (2010)
  • Author(s): Kurita D., Une M., Muto A., Himeno H.
  • Organizer: The 3rd International Symposium on Protein Community
  • Place of Presentation: Nara
• [Presentation] Molecular mechanism of the early stage of trans-translation. (2010)
  • Author(s): Kurita D., Une M., Muto A., Himeno H.
  • Organizer: 7th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Yokohama
• [Presentation] トランス・トランスレーション初期過程におけるC末端tailの働き (2010)
  • Author(s): 栗田大輔、宇根理高、武藤〓、姫野俵太
  • Organizer: 第12回RNAミーティング
  • Place of Presentation: 東京
• [Presentation] リボソーム成熟因子RsgA/YjeQの構造機能解析 (2010)
  • Author(s): 竹本千重、Sean Connell、長谷要一、直枝智恵子、王宏飛、上西達也、菊池岳志、平田侑也、栗田大輔、武藤〓、武藤裕、横山茂之、Paola Fucini、姫野俵太
  • Organizer: 第12回RNAミーティング
  • Place of Presentation: 東京
• [Presentation] tRNAとmRNAの両方の機能を持つtmRNA (2010)
  • Author(s): 姫野俵太
  • Organizer: 9回新しい RNA/RNP を見つける会
  • Place of Presentation: 弘前
• [Presentation] 無細胞トランス・トランスレーション系における SmpBのC末端tailの働き (2010)
  • Author(s): 栗田大輔、武藤〓、姫野俵太
  • Organizer: 第5回無細胞生命科学研究会
  • Place of Presentation: 岡山
• [Presentation] tRNA/mRNAハイブリッドとtRNA/mRNA擬態タンパク質によるtrans-translation (2010)
  • Author(s): 姫野俵太、栗田大輔、宇根理高、武藤〓
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] Molecular mimicry of tRNA/mRNA during trans-translation. (2010)
  • Author(s): Kurita, D., Une, M., Muto, A., Himeno, H.
  • Organizer: ASBMB 2010 Annual Meeting
  • Place of Presentation: Anaheim Convention Center, Anaheim, USA
• [Presentation] Structural studies for RsgA/YjeQ, a ribosome maturation factor (2010)
  • Author(s): Takemoto, C., Connell, S.R., Hase, Y., Naoe, C., Wang, H., Kaminishi, T., Kikuchi, T., Hirata, Y., Kurita, D., Muto, A., Muto, Y., Yokoyama, S., Himeno, H.
  • Organizer: Ribosomes 2010 Meeting
  • Place of Presentation: Palazzo del Popolo, Orvieto, Italy
• [Presentation] Molecular mimicry by tmRNA/SmpB during traps-translation (2010)
  • Author(s): Kurita, D., Une, M., Muto, A., Himeno, H.
  • Organizer: Ribosomes 2010 Meeting
  • Place of Presentation: Palazzo del Popolo, Orviet D, Italy
• [Presentation] the mutation of the C.elegans genomic locus encoding a snoRNA has a dfect in the pre-rRNA processing (2010)
  • Author(s): Ushida, C., Hokii, Y., Sasano, Y., Sato, M., Sakamoto, H., Himeno, H., Muto, A., Fuj iwara, T.
  • Organizer: Fifteenth Annual Meeting of the RNA Society
  • Place of Presentation: University of Washington, Seattle
• [Presentation] トランス・トランスレーション初期過程におけるC末端tailの働き (2010)
  • Author(s): 栗田大輔、宇根理高、武藤〓、姫野俵太
  • Organizer: 第12回RNAミーティング
  • Place of Presentation: 一ツ橋記念講堂(東京都)
• [Presentation] リボソーム成熟因子RsgA/YjeQの構造機能解析 (2010)
  • Author(s): 竹本千重、Sean Connell、長谷要一、直枝智恵子、王宏飛、上西達也、菊池岳志、平田侑也、栗田大輔、武藤豆、武藤裕、横山茂之、Paola Fucini、姫野俵太
  • Organizer: 第12回RNAミーティング
  • Place of Presentation: 一ツ橋記念講堂(東京都)
• [Presentation] tRNAとmRNAの両方の機能を持つtmRNA (2010)
  • Author(s): 姫野俵太
  • Organizer: 第9回新しいRNA/RNPを見つける会
  • Place of Presentation: アソベの森いわき荘(弘前市)
• [Presentation] 無細胞トランス・トランスレーション系におけるSmpBのC末端tailの働き (2010)
  • Author(s): 栗田大輔、武藤〓、姫野俵太
  • Organizer: 第5回無細胞生命科学研究会
  • Place of Presentation: 剛山大学(岡山市)
• [Presentation] Trans-translation by tmRNA/SmpB (2010)
  • Author(s): Kurita, D., Une, M., Muto, A., Himeno, H.
  • Organizer: The 3rd International Symposium on Protein Community
  • Place of Presentation: Hotel Nikko Nara, Nara
• [Presentation] Molecular mechanism of the early stage of trans-translation. (2010)
  • Author(s): Kurita, D., Une, M., Muto, A., Himeno, H.
  • Organizer: 7th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: はまぎんボール・ヴィアマーレ、Yokohama
• [Presentation] tRNA/mRNAハイブ-リッドとtRNA/mRNA擬態タンパク質によるtrans-translation (2010)
  • Author(s): 姫野俵太、栗田大輔、宇根理高、武藤〓
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
• [Presentation] Role of the C-terminal tail of SmpB during tans-translation. (2010)
  • Author(s): Kurita, D., Une, M., Muto, A., Himeno, H.
  • Organizer: 23^<rd> tRNA workshop.
  • Place of Presentation: Aveiro, Portugal
• [Presentation] Removal of robosome small subunit-dependent GTPase confers salt resistance on Escherichia coli cells. (2009)
  • Author(s): Hase Y., Yokoyama S., Muto A., Himeno H.
  • Organizer: 4th International Congress on Stress Responses in Biology and Medicine.
  • Place of Presentation: Sapporo
• [Presentation] Trans-translation by tmRNA and SmpB. (2009)
  • Author(s): Une M., Kurita D., Muto A., Himeno H.
  • Organizer: The sixth International Sympojium on Nucleic Acids Chemistry
  • Place of Presentation: Takayama
• [Presentation] Physiological role of RsgA in ribosome biogenesis. (2009)
  • Author(s): Hase Y., Yokoyama S., Kimura T., Goto S., Muto A., Himeno H.
  • Organizer: The sixth International Sympojium on Nucleic Acids Chemistry
  • Place of Presentation: Takayama
• [Presentation] リボソーム小サブユニット依存GTPaseの欠損による塩ストレス耐性の獲得 (2009)
  • Author(s): 長谷要一、横山晋一郎、武藤あきら、姫野俵太
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
• [Presentation] リボソーム小サブユニット依存GTPaseの欠損による塩ストレス耐性の獲得 (2009)
  • Author(s): 長谷要一、横山晋一郎、武藤あきら、姫野俵太
  • Organizer: 第11回RNAミーティング
  • Place of Presentation: 新潟
• [Presentation] 部位特異的ヒドロキシラジカルプロービングを用いたRsgAとリボソームの相互作用の解析 (2009)
  • Author(s): 菊地岳志、平田侑也、栗田大輔、長谷要一、木村天胤、武藤あきら、姫野俵太
  • Organizer: 第11回RNAミーティング
  • Place of Presentation: 新潟
• [Presentation] tmRNA機能解析のための無細胞翻訳実験系の構築 (2009)
  • Author(s): 高田一馬、直枝智恵子、川添将仁、横山茂之、姫野俵太、竹本千重
  • Organizer: 第11回RNAミーティング
  • Place of Presentation: 新潟
• [Presentation] Physiological role of RsgA in ribosome biogenesis. (2009)
  • Author(s): Hase, Y., Yokoyama, S., Kimura, T., Goto, S., Muto, A., Himeno, H.
  • Organizer: 6th International Symposium on Nucleic Acids Chemistry.
  • Place of Presentation: Takayama
• [Presentation] Trans-translation by tmRNA and SmpB. (2009)
  • Author(s): Une, M., Kurita, D., Muto, A., Himeno, H.
  • Organizer: 6th International Symposium on Nucleic Acids Chemistry.
  • Place of Presentation: Takayama
• [Presentation] Molecular mimicry of tRNA+mRNA during trans-translation. (2008)
  • Author(s): Kurita D., Konno T., Takada K., Muto A., Himeno H.
  • Organizer: 13th Annual Meeting of the RNA Society
  • Place of Presentation: Berlin
• [Presentation] リボソーム小サブユニット依存GTPase RsgA欠損による浸透圧耐性の獲得 (2008)
  • Author(s): 長谷要一・横山晋一郎・武藤あきら・姫野俵太
  • Organizer: 第10回RNAミーティング
  • Place of Presentation: 札幌
• [Presentation] tRNA+mRNAのキメラRNA"と"tRNA+mRNA擬態タンパク質"によるtrans-translation. (2008)
  • Author(s): 栗田大輔・今野貴之・高田一馬・武藤 あきら・姫野俵太
  • Organizer: 第10回RNAミーティング
  • Place of Presentation: 札幌
• [Presentation] 枯草菌tmRNAによるtrans-translation反応のカタボライト抑制への関与 (2008)
  • Author(s): 氏家博美・松谷誠子・戸松恒・藤原愛・姫野俵太・武藤あきら
  • Organizer: 第10回RNAミーティング
  • Place of Presentation: 札幌
• [Presentation] "tRNA+mRNAのキメラRNA"と"tRNA+mRNA擬態タンパク質"によるtrans-translation (2008)
  • Author(s): 栗田大輔
  • Organizer: 第10回RNAミーティング
  • Place of Presentation: 札幌
• [Presentation] Molecular mimicry of tRNA+mRNA during trans-translation (2008)
  • Author(s): Kurita, D.
  • Organizer: 30th annual meeting of the RNA society
  • Place of Presentation: Berlin
• [Book] 生命化学II 第2版 -遺伝子の働きとその応用- (2011)
  • Author(s): 渡辺公綱、姫野俵太
  • Publisher: 丸善
• [Book] mRNAプログラム 多様性と非対称性の獲得戦略(稲田利文、大野睦人, 編)(2つのtRNA/mRNAハイブリッド-トランス・トランスレーション-) (2010)
  • Author(s): 姫野俵太、栗田大輔、武藤あきら
  • Publisher: 共立出版
• [Book] mRNAプログラム多様性と非対称性の獲得戦略 (2010)
  • Author(s): 姫野俵太、栗田大輔、武藤〓(稲田利文、大野睦人編)
  • Total Pages: 6
  • Publisher: 共立出版
• [Book] 生命化学II第2版-遺伝子の働きとその応用- (2010)
  • Author(s): 渡辺公綱、姫野俵太
  • Total Pages: 290
  • Publisher: 丸善出版
• [Book] 改訂第3版分子生物学イラストレイテッド(田村隆明、山本雅, 編)(翻訳) (2009)
  • Author(s): 渡辺公綱、鈴木勉、姫野俵太
  • Publisher: 羊土社
• [Book] Protein Biosynthesis(Toma E. Esterhouse & Lado B. Petrinos Eds.)(Trans-translation by tmRNA and a protein mimicking tRNA and mRNA.) (2009)
  • Author(s): Himeno H., Kurita D., Muto A.
  • Publisher: Nova Science Publishers Inc
• [Book] 改訂第3版分子生物学イラストレイテッド (2009)
  • Author(s): 渡辺公綱
  • Total Pages: 9
  • Publisher: 羊土社
• [Book] Protein Biosynthesis (2009)
  • Author(s): Himeno, H.
  • Total Pages: 39
  • Publisher: Nova Science Publishers Inc
• [Book] ベーシックマスター生化学(大山隆, 監修、西川一八、清水光弘, 編)(窒素同化とアミノ酸代謝) (2008)
  • Author(s): 姫野俵太
  • Publisher: オーム社
• [Book] キーワード:蛋白質の一生(遠藤斗志也、小椋光、永田和宏、森和俊、田口英樹、吉田賢右, 編)(トランストランスレーション) (2008)
  • Author(s): 姫野俵太、武藤あきら
  • Total Pages: 975
  • Publisher: 共立出版
• [Remarks] ホームページ等
  • URL: http://nature.cc.hirosaki-u.ac.jp/kohou2/public/oyo/h.himeno/index.htm