Molecular Mechanism of trans-translation
| Project/Area Number |
20380057
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Hirosaki University |
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Principal Investigator |
HIMENO Hyouta Hirosaki University, 農学生命科学部, 教授 (80208785)
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| Co-Investigator(Kenkyū-buntansha) |
MUTO Akira 弘前大学, 農学生命科学部, 研究員 (80034635)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Keywords | 遺伝子発現 / tmRNA / trans-translation / ribosome / molecular mimicry / SmpB / translation / EF-Tu |
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| Research Abstract |
Trans-translation is an irregular translation system, in which tmRNA (transfer and messenger RNA) rescues the ribosome stalled at the 3' end of truncated mRNA lacking a termination codon to produce a chimeric polypeptide from two messages on truncated mRNA and tmRNA. It provides an incomplete nascent polypeptide from a truncated mRNA with a tag for degradation to promote degradation of truncated mRNA. This project revealed the molecular mechanism of the early stage of trans-translation based on our proposal that SmpB, a tmRNA binding protein, mimics both tRNA and mRNA.
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Report
(4 results)
Research Products
(79 results)
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[Journal Article] A small nucleolar RNA functions in rRNA processing in C. elegans.2010
Author(s)
Hokii Y., Sasano Y., Sato M., Sakamoto H., Sakata K., Shingai R., Taneda A., Oka S., Himeno H. Muto A., Fujiwara T., Ushida C.
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Journal Title
Nucleic Acids Research 38
Pages: 5909-5918
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[Journal Article] A small nucleolar RNA functions in rRNA processing in C.elegans2010
Author(s)
Hokii, Y., Sasano, Y., Sato, M., Sakamoto, H., Sakata, K., Shingai, R., Taneda, A., Oka, S., Himeno, H., Muto, A., Fujiwara, T., Ushida, C.
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Journal Title
Nucleic Acids Res.
Volume: 38
Pages: 5909-5918
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Peer Reviewed
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[Journal Article] Physiological role of RsgA in ribosome biogenesis.2009
Author(s)
Hase, Y., Yokoyama, S., Kimura, T., Goto, S., Muto, A., Himeno, H.
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Journal Title
Nucleic Acids Symp.Ser. 53
Pages: 307-308
Related Report
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[Presentation] Structural studies for RsgA/YjeQ, a ribosome maturation factor.2010
Author(s)
Takemoto C., Connell S.R., Hase, Y., Naoe C., Wang H., Kaminishi T., Kikuchi T., Hirata Y., Kurita D., Muto A., Muto Y., Yokoyama S., Himeno H.
Organizer
Ribosomes 2010 Meeting
Place of Presentation
Orvieto, Italy
Related Report
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[Presentation] Structural studies for RsgA/YjeQ, a ribosome maturation factor2010
Author(s)
Takemoto, C., Connell, S.R., Hase, Y., Naoe, C., Wang, H., Kaminishi, T., Kikuchi, T., Hirata, Y., Kurita, D., Muto, A., Muto, Y., Yokoyama, S., Himeno, H.
Organizer
Ribosomes 2010 Meeting
Place of Presentation
Palazzo del Popolo, Orvieto, Italy
Related Report
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[Presentation] the mutation of the C.elegans genomic locus encoding a snoRNA has a dfect in the pre-rRNA processing2010
Author(s)
Ushida, C., Hokii, Y., Sasano, Y., Sato, M., Sakamoto, H., Himeno, H., Muto, A., Fuj iwara, T.
Organizer
Fifteenth Annual Meeting of the RNA Society
Place of Presentation
University of Washington, Seattle
Related Report
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[Presentation] Physiological role of RsgA in ribosome biogenesis.2009
Author(s)
Hase, Y., Yokoyama, S., Kimura, T., Goto, S., Muto, A., Himeno, H.
Organizer
6th International Symposium on Nucleic Acids Chemistry.
Place of Presentation
Takayama
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