Target identification of vauolating compound, vicenistatin.

• Project/Area Number: 20380065
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: University of Tsukuba
• Principal Investigator: USUI Takeo University of Tsukuba, 大学院・生命環境科学研究科, 准教授 (60281648)
• Co-Investigator(Renkei-kenkyūsha): KANOH Naoki 東北大学, 大学院・薬学研究科, 准教授 (40317293)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2008: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: 液胞化 / 標的分子 / Chemical Biology

Research Abstract

Vicenistatin is an novel vacuolating compound, but its molecular mechanism has remained to be revealed. We found that vicenistatin interfered with the upstream factors of PIKfyve, a PI3P 5-kinase, or regulators involved in PI-turnover.

Research Products

• [Journal Article] Irciniastatin A induces JNK activation that is involved in caspase-8-dependent apoptosis via the mitochondrial pathway. (2010)
  • Author(s): Chinen T., Nagumo Y., Watanabe T., Imaizumi T., Shibuya M., Kataoka T., Kanoh N., Iwabuchi Y., Usui T.
  • Journal Title: Toxicol. Lett. 199 Pages: 341-356
  • Peer Reviewed
• [Journal Article] Application of proteomic profiling based on 2-DIGE for classification of compounds according to the mechanism of action. (2010)
  • Author(s): Muroi M., Kazami S., Noda K., Kondo H., Takayama H., Kawatani M., Usui T., Osada H.
  • Journal Title: Chem.Biol. 17 Pages: 460-470
  • Peer Reviewed
• [Journal Article] Application of proteomic profiling based on 2-DIGE for classification of compounds according-to the mechanism of action (2010)
  • Author(s): Muroi, M., et al
  • Journal Title: Chemistry & Biology Volume: 28 Pages: 460-470
  • Peer Reviewed
• [Journal Article] Structure-activity relationship study of glaziovianin A against cell cycleprogressi on and spindleformation of HeLa S3 cells. (2010)
  • Author(s): Ikedo, A., et al.
  • Journal Title: Bioorganic Medicinal Chemistry Letters Volume: 20 Pages: 5402-5404
  • Peer Reviewed
• [Journal Article] Irciniastatin A induces JNK activation that is involved incaspase-8-dependent apoptosis vra the mitochondrial pathway (2010)
  • Author(s): Chinen, T., et al.
  • Journal Title: Toxicology Letters Volume: 199 Pages: 34-346
  • Peer Reviewed
• [Journal Article] Garbled messages and corrupted translations (2010)
  • Author(s): Schneider-Poetsch T., et al
  • Journal Title: Nature Chemical Biology 6 Pages: 189-198
  • Peer Reviewed
• [Journal Article] Syntheses and biological evaluation of irciniastatin A and the C1-C2 alkyne analogue. (2010)
  • Author(s): Watanabe T., et al.
  • Journal Title: Organic Letters 12 Pages: 1040-1043
  • Peer Reviewed
• [Journal Article] The cytotoxic macrolide FD-891 induces caspase-8-dependent mitochondrial release of cytochrome c and subsequent apoptosis in human leukemia Jurkat cells. (2009)
  • Author(s): Inaba S., et al.
  • Journal Title: Journal of Antibiotics 62 Pages: 507-512
  • Peer Reviewed
• [Journal Article] Epoxyquinol B shows antiantiogenic and antitumor effects by inhi biting VEGFR2. EGFR. FGFR. and PDGFR. (2008)
  • Author(s): Kamiyama H, et al.
  • Journal Title: Oncology Research 17 Pages: 11-21
  • Peer Reviewed
• [Journal Article] Fungal metabolite, epoxyquinol B, crosslinks proteins by epoxy-thiol conjugation. (2008)
  • Author(s): Kamiyama H, et al.
  • Journal Title: Journal of Antibiotics 61 Pages: 94-97
  • Peer Reviewed
• [Journal Article] Synthesis and biological activities of reveromycin A and spirofungin A derivatives. (2008)
  • Author(s): Shimizu T, et al.
  • Journal Title: Bioorganic Medicinal Chemistry Letters 18 Pages: 3756-3760
  • Peer Reviewed
• [Presentation] 蛋白質-蛋白質相互作用阻害剤の解析 (2010)
  • Author(s): 臼井健郎
  • Organizer: 日本農芸化学会東北北海道支部合同第11回若手シンポジウム
  • Place of Presentation: モンタナリゾート(宮城県)
  • Year and Date: 2010-11-26
• [Presentation] Glaziovianin A, a new inhibitor of microtubule dynamics (2010)
  • Author(s): Usui, T.
  • Organizer: International Symposium on Cell Functions Mediated by Small Molecules
  • Place of Presentation: 筑波大学
  • Year and Date: 2010-11-08
• [Presentation] マーカーレス遺伝子破壊によるフォワードケミカルバイオロジー研究に向けた薬剤超感受性酵母の作成と利用 (2010)
  • Author(s): 臼井健郎
  • Organizer: 酵母研究会
  • Place of Presentation: 小西酒造(株)(兵庫県)
  • Year and Date: 2010-03-09
• [Presentation] マーカーレス遺伝子破壊によるフォワードケミカルバイオロジー研究に向けた薬剤超感受性酵母の作成と利用 (2010)
  • Author(s): 臼井健郎
  • Organizer: 酵母研究会
  • Place of Presentation: 小西酒造(株)本社(兵庫県)
  • Year and Date: 2010-03-09
• [Presentation] 蛋白質一蛋白質相互作用阻害剤の解析 (2010)
  • Author(s): 臼井健郎
  • Organizer: 日本農芸化学会東北北海道支部合同第11回若手シンポジウム
  • Place of Presentation: モンタナリゾート岩沼(宮城県岩沼市)
• [Book] Protein in Targeting with Small Molecules(Osada H.ed.)(Chapter 12 "Data on Small Molecules and Their Target Proteins") (2009)
  • Author(s): Usui T., Saito A., Osada H.
• [Book] Chapter 12"Data on Small Molecules and Their Target Proteins"in Prote in Targeting with Small Molecules(H.Osada, ed.) (2009)
  • Author(s): Usui T., Saito A., Osada H
  • Total Pages: 289
  • Publisher: Wiley
• [Remarks] ホームページ
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• [Remarks]
  • URL: http://www.sakura.cc.tsukuba.ac.jp/~usui/index/index.html
• [Remarks]
  • URL: http://www.sakura.cc.tsukuba.ac.jp/~usui/index/index.html