Development of chemical biology based on allosamidin molecule

• Project/Area Number: 20380066
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: SAKUDA Shohei The University of Tokyo, 大学院・農学生命科学研究科, 准教授 (80192087)
• Co-Investigator(Renkei-kenkyūsha): INOUE Hiromasa 鹿児島大学, 大学院・医歯学総合研究科, 教授 (30264039)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000); Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2008: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 生物活性物質 / キチナーゼ / 喘息 / キチナーゼ様タンパク質 / アロサミジン / 放線菌 / 二次代謝 / 生合成 / キチナーゼ阻害物質 / Ym1 / デメチルアロサミジン / キチナーゼ阻害剤 / キチン / 二次代謝産物

Research Abstract

Allosamidin, a chitinase inhibitor, shows anti-asthmatic and chitinase production promoting activities toward mammals and Streptomyces bacteria, respectively. Allosamidin photo-affinity probe was used to identify a chitinase-like protein as a key allosamidin-binding molecule for anti-asthmatic activity of allosamidin. Expression systems to obtain recombinant candidate proteins for allosamidin's target molecule including the chitinase-like protein were constructed. Gene cluster responsible for allosamidin biosynthesis was obtained, which is useful for investigating the role of allosamidin in environment.

Research Products

• [Journal Article] (2011)
  • Author(s): Yosuke Sato, Shigeo Suzuki, Seiko Muraoka, Naoya Kikuchi, Naotaka Noda, Takafumi Matsumoto, Hiromasa Inoue, Hiromichi Nagasawa, Shohei Sakuda
  • Journal Title: Bioorg.Med.Chem. 19 Pages: 3054-3059
  • Peer Reviewed
• [Journal Article] Transglycosylation reaction catalyzed by a class V chitinase from cycad (2010)
  • Author(s): Toki Taira, Maho Fujiwara, Nicole Dennhart, Hiroko Hayashi, Shoko Onaga, Takayuki Ohnuma, Thomas Letzel, Shohei Sakuda, Tamo Fukamizo
  • Journal Title: Cycas revolute : A study involving site-directed mutagenesis, HPLC, and real-time ESI-MS. Biochimica et Biophysica Acta 1804 Pages: 668-675
  • Peer Reviewed
• [Journal Article] The roles of three Serratia marcescens chitinases in chitin conversion are reflected in different thermodynamic signatures of allosamidin binding. (2010)
  • Author(s): Jamil Baban, Salima Fjeld, Shohei Sakuda, Vincent G.H.Eijsink, Morten Soerlie
  • Journal Title: J.Phys.Chem.B 114 Pages: 6144-6149
  • Peer Reviewed
• [Journal Article] Effect of enzyme processivity on the efficacy of a competitive chitinase inhibitor. (2010)
  • Author(s): Henrik Zakariassen, Laila Klemetsen, Shohei Sakuda, Gustav Vaaje-Kolstad, Kjell M.Varum, Morten Sorlie, Vincent G.Eijsink
  • Journal Title: Carbohydrate Polymers 82 Pages: 779-785
  • Peer Reviewed
• [Journal Article] Effect of enzyme processivity on the efficacy of a competitive chitinase inhibitor (2010)
  • Author(s): Henrik Zakariassen
  • Journal Title: Carbohydrate Polymers Volume: 82巻 Pages: 779-785
  • Peer Reviewed
• [Journal Article] The roles of three Serratia marcescens chitinases in chitin conversion are reflected in different thermodynamic signatures of allosamidin binding (2010)
  • Author(s): amil Baban
  • Journal Title: J.Phys.Chem.B Volume: 114巻 Pages: 6144-6149
  • Peer Reviewed
• [Journal Article] Transglycosylation reaction catalyzed by a class V chitinase from cycad, Cycas revolute : A study involving site-directed mutagenesis, HPLC, and real-time ESI-MS (2010)
  • Author(s): Told Taira
  • Journal Title: Biochimica et Biophysica Acta Volume: 1804 Pages: 668-675
  • Peer Reviewed
• [Journal Article] Transglycosylation reaction catalyzed by a class V chitinase from cycad, Cycas revolute : A study involving site-directed mutagenesis, HPLC, and real-time ESI-MS (2010)
  • Author(s): Toki Taira, et al.
  • Journal Title: Biochimica et Biophysica Acta 1804 Pages: 668-675
  • Peer Reviewed
• [Journal Article] Chitinase gene expression in response to environmental stresses in Arabidopsis thaliana : Chitinase inhibitor allosamidin enhances the stress (2009)
  • Author(s): Yasuhiro Takenaka, Sachiko Nakano, Masahiro Tamoi, Shohei Sakuda, Tamo Fukamizo
  • Journal Title: Biosci.Biotechnol.Biochem. 73 Pages: 1066-1071
  • Peer Reviewed
• [Journal Article] Demethylallosamidin, a chitinase inhibitor, suppresses airway inflammation and hyperresponsiveness. (2009)
  • Author(s): Takafumi Matsumoto, Hiromasa Inoue, Yosuke Sato, Yoshihiro Kita, Takako Nakano, Naotaka Noda, Miyuki Eguchi-Tsuda, Atsushi Moriwaki, Keiko Kan-o, Koichiro Matsumoto, Takao Shimizu, Hiromichi Nagasawa, Shohei Sakuda, Yoichi Nakanishi
  • Journal Title: Biochem.Biophys.Res.Commun. 390 Pages: 103-108
  • Peer Reviewed
• [Journal Article] demethylallosamidin, a chitinase inhibitor, suppresses airway inflammation and hyperresponsiveness. (2009)
  • Author(s): Takafumi Matsumoto, et al.
  • Journal Title: Biochem.Biophys.Res.Commun. 390 Pages: 103-108
  • Peer Reviewed
• [Journal Article] Chitinase gene expression in response to environmental stresses in Arabidopsis thaliana : Chitinase inhibitor allosamidin enhances the stress (2009)
  • Author(s): Yasuhiro Takenaka, et al.
  • Journal Title: Biosci. Biotechnol. Biochem. 73 Pages: 1066-1071
  • Peer Reviewed
• [Journal Article] Chtinase inhibitor allosamidin promotes chitinase production of Streptomyces generally (2008)
  • Author(s): Shigeo Suzuki, Eiyu Nakanishi, Keiko Furihata, Katsushiro Miyamoto, Hiroshi Tsujibo, Takeshi Watanabe, Yasuo Ohnishi, Sueharu Horinouchi, Hiromichi Nagasawa, Shohei Sakuda
  • Journal Title: Int.J.Biol.Macromol. 43 Pages: 13-19
  • Peer Reviewed
• [Journal Article] Chtinase inhibitor allosamidin promotes chitinase production of Strepotmyces generally (2008)
  • Author(s): Shigeo Suzuki et al.
  • Journal Title: International Journal of Biological Macromolecules 43 Pages: 13-19
  • Peer Reviewed
• [Presentation] アロサミジンのキチナーゼ生産促進および抗喘息作用の分子機構解析 (2010)
  • Author(s): 作田庄平
  • Organizer: 第52回天然有機化合物討論会
  • Place of Presentation: 静岡
  • Year and Date: 2010-09-29
• [Presentation] アロサミジンのキチナーゼ生産促進および抗喘息作用の分子機構解析 (2010)
  • Author(s): 作田庄平
  • Organizer: 第52回天然有機化合物討論会
  • Place of Presentation: グランシップ(静岡)
  • Year and Date: 2010-09-29
• [Presentation] マウスのキチナーゼおよびキチナーゼ様タンパク質とアロサミジン類 (2010)
  • Author(s): 村岡聖子
  • Organizer: 第24回キチン・キトサンシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-07-13
• [Presentation] マウスのキチナーゼおよびキチナーゼ様タンパク質とアロサミジン類 (2010)
  • Author(s): 村岡聖子
  • Organizer: 第24回キチン・キトサンシンポジウム
  • Place of Presentation: 東京大学弥生講堂(東京)
  • Year and Date: 2010-07-13
• [Presentation] アロサミジン類の抗喘息作用における作用機構解析 (2010)
  • Author(s): 村岡聖子
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] Strong anti-asthmatic and Ym1-binding activities of demethylallosamidin (2009)
  • Author(s): 作田庄平
  • Organizer: 11th International Conference on Chitin & Chitosan
  • Place of Presentation: Taipei
  • Year and Date: 2009-09-08
• [Presentation] Strong anti-asthmatic and Ym1-binding activities of demethylallosamidin (2009)
  • Author(s): Shohei Sakuda, et al.
  • Organizer: 11th International Conference on Chitin & Chitosan
  • Place of Presentation: Taipei 台湾
  • Year and Date: 2009-09-08
• [Presentation] アロサミジン生合成酵素methyltransferaseの精製に関する研究 (2009)
  • Author(s): 田川直史
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] アロサミジン生合成酵素methyltransferaseの精製に関する研究 (2009)
  • Author(s): 田川直史他
  • Organizer: 2009年度日本農芸化学会大会
  • Place of Presentation: マリンメッセ福岡
  • Year and Date: 2009-03-29
• [Presentation] 多彩な顔を持つアロサミジン (2008)
  • Author(s): 作田庄平
  • Organizer: 第22回キチン・キトサンシンポジウム特別セッション
  • Place of Presentation: 新潟
  • Year and Date: 2008-08-05
• [Presentation] 多彩な顔を持つアロサミジン (2008)
  • Author(s): 作田庄平
  • Organizer: 第22回キチン・キトサンシンポジウム
  • Place of Presentation: 朱鷺メッセ
  • Year and Date: 2008-08-05
• [Presentation] アロサミジン生合成酵素methyltransferaseの精製 (2008)
  • Author(s): 田川直史他
  • Organizer: 2008年度日本放線菌学会大会
  • Place of Presentation: フルーツパーク富士屋ホテル
  • Year and Date: 2008-07-10
• [Book] キチン・キトサン研究(マウスのキチナーゼおよびキチナーゼ様タンパク質とアロサミジン類)17 (2011)
  • Author(s): 村岡聖子、野田直孝、井上博雅、菊池直也、長澤寛道、作田庄平
• [Book] Natural Products : Chemical Ecology Edited by Kenji Mori(Toxins of microorganisms)Comprehensive Natural Products Chemistry Vol.3 (2010)
  • Author(s): Shohei Sakuda, Makoto Kimura
  • Publisher: Elsevier Limited
• [Book] Binomium Chitin-Chitinase: Emerging issues Edited by Salvatore Musumeri and Maurizio G.Paoletti(Chitinase inhibitors and its significance in biology) (2009)
  • Author(s): 作田庄平
  • Total Pages: 20
  • Publisher: Nova Science Publishers, Inc.
• [Book] The biochemical significance of allosamidins as chitinase inhibitors in Biononium Chitin-Chitinase : Emerging issues (2009)
  • Author(s): Shohei Sakuda
  • Publisher: Nova Science Publishers, Inc.
• [Book] "The biochemical significance of allosamidins as chitinase inhibitors" in Biononium Chitin-Chitinase:Emerging issues″edited by Salvatore Musumeci and Maurizio G. Paletti, in press (2009)
  • Author(s): Shohei Sakuda
  • Publisher: Nova Science Publishers, Inc.
• [Remarks]
  • URL: http://www.a.u-tokyo.ac.jp/researchers/index.html