| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2008: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Research Abstract |
To identify the binding site of quinazoline and Dlac-acetogenin in bovine heart mitochondrial complex-I (NADH-quinone oxidoreductase), we synthesized photoreactive probes [^<125>I]AzQ and [^<125>I]DAA, respectively. We revealed that [^<125>I]AzQ binds at the interface of the 49 kDa and ND1 subunits. On the other hand, careful examination of the fragmentation patterns of the ND1 subunit cross-linked by [^<125>I]DAA strongly suggested that the cross-linked residues are located at two different sites in the third matrix-side loop connecting the 5th and 6th transmembrane helices.
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