Exploring the function of membrane domain of mitochondrial complex-I on the basis of synthesis of inhibitor probes
| Project/Area Number |
20380068
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
MIYOSHI Hideto Kyoto University, 大学院・農学研究科, 教授 (20190829)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2008: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Keywords | 細胞機能調節物質 / ミトコンドリア / 複合体-I / 光親和性標識 / 阻害剤 / アセトゲニン / ND1サブユニット / 呼吸鎖酵素 / ペプチド化学 / 生体エネルギー |
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| Research Abstract |
To identify the binding site of quinazoline and Dlac-acetogenin in bovine heart mitochondrial complex-I (NADH-quinone oxidoreductase), we synthesized photoreactive probes [^<125>I]AzQ and [^<125>I]DAA, respectively. We revealed that [^<125>I]AzQ binds at the interface of the 49 kDa and ND1 subunits. On the other hand, careful examination of the fragmentation patterns of the ND1 subunit cross-linked by [^<125>I]DAA strongly suggested that the cross-linked residues are located at two different sites in the third matrix-side loop connecting the 5th and 6th transmembrane helices.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Characterization of the ubiquinone binding site in alternative NADH-quinone oxidoreductase of Saccharomyces cerevisiae by photoaffinity labeling2010
Author(s)
Murai, M., Yamashita, T., Senoh, M., Mashimo, Y., Kataoka, M., Kosaka, H., Matsuno-Yagi, A., Yagi, T., Miyoshi, H.
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Journal Title
Biochemistry 49
Pages: 2973-2980
Related Report
Peer Reviewed
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[Journal Article] Synthesis and characterization of new piperazine-type inhibitors for mitochondrial NADH-ubiquinone oxidoreductase(complex I)2008
Author(s)
Ichimaru, N., Murai, M., Kakutani, N, Kako, J., Ishihara, A., Nakagawa, Y., Nishioka, T., Yagi, T., Miyoshi
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Journal Title
Biochemitsry 47
Pages: 10816-10826
Related Report
Peer Reviewed
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[Journal Article] Dynamic fhnction of the spacer region of acetogen ins in the inhibition of bovine mitochondrial NADH-ubiquinone oxidoreductase (complex I)2008
Author(s)
Abe, M., Kubo, A., Yamamoto, S., Hatoh, Y., Murai, M., Hattori, Y., Makabe, H., Nishioka, T., Miyoshi, H.
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Journal Title
Biochemistry 47
Pages: 6260-6266
Related Report
Peer Reviewed
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