Development of a synthetic method for hydrophobic glycoprotein and its application to saposin synthesis
| Project/Area Number |
20380069
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Tokai University |
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Principal Investigator |
HOJO Hironobu Tokai University, 工学部, 教授 (00209214)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAHARA Yoshiaki 東海大学, 工学部, 教授 (50087574)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2008: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 疎水性糖タンパク質 / セグメント縮合 / O-アシルイソペプチド / O-アシルイソペプチド法 / ネイティブケミカルライゲーション法 / アジド基 / サポシン / 難溶性糖ペプチド / O-acyl isopeptide / Saposin / ペプチドチオエステル |
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| Research Abstract |
The synthesis of highly hydrophobic glycoproteins, such as membrane proteins and lipid-binding proteins, is extremely difficult even with the use of efficient segment coupling methods. The main difficulty is that the segments usually retain low solubility during peptide synthesis, purification and ligation steps. To overcome this problem, a method to enhance the solubility of segments during peptide synthesis and purification was developed and applied to the synthesis of lipid-binding protein, saposin C.
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Report
(4 results)
Research Products
(81 results)
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[Journal Article] Synthesis of the sphingolipid activator protein, saposin C, using an azido-protected O-acyl isopeptide as an aggregation-disrupting element2011
Author(s)
H.Hojo, H.Katayama, C.Tano, Y.Nakahara, A.Yoneshige, J.Matsuda, Y.Sohma, Y.Kiso, Y.Nakahara
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Journal Title
Tetrahedron Lett.
Volume: 52
Pages: 635-639
Related Report
Peer Reviewed
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[Journal Article] Direct binding of gangliosides to Helicobacter pylori vacuolating cytotoxin (VacA) neutralizes its toxin activity2010
Author(s)
A.Wada, M.Hasegawa, P.-F.Wong, E.Shirai, L.-J.Tan, R.Llanes, H.Hojo, E.Yamasaki, A.Ichinose, Y.Ichinose, M.Senba
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Journal Title
Glycobiology 20
Pages: 668-678
NAID
Related Report
Peer Reviewed
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[Journal Article] Direct binding of gangliosides to Helicobacter pylori vacuolating cytotoxin (VacA) neutralizes its toxin activity.2010
Author(s)
Wada, A., Hasegawa, M., Wong, P.-F., Shirai, E., Shirai, N., Tan, I.J., Llanes, R., Hojo, H., Yamasaki, E., Ichinose, A., Ichinose, Y., Senba, M.
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Journal Title
Glycobiology
Volume: 20
Pages: 668-678
NAID
Related Report
Peer Reviewed
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[Journal Article] Solid-phase synthesis of glycopeptide carrying a tetra-N-acetyllactosamine containing core 2 decasaccharide, Tetrahedron, 66 1742-1759(2010)2010
Author(s)
Ueki, A., Takano, Y., Kobayashi, A., Nakahara, Y., Hojo, H., Nakahara, Y.
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Journal Title
Tetrahedron
Volume: 66
Pages: 1742-1759
Related Report
Peer Reviewed
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[Journal Article] Direct binding of gangliosides to Helicobacter pylori vacuolating cytotoxin (VacA) neutralizes its toxin activity.2010
Author(s)
A.Wada, M.Hasegawa, P.-F.Wong, E.Shirai, N.Shirai, Li-Jing Tan, R.Llanes, H.Hojo, E.Yamasaki, A.Ichinose, Y.Ichinose, M.Senba.
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Journal Title
NAID
Related Report
Peer Reviewed
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[Presentation] Synthesis of glycoprotein by the segment condensation method.2010
Author(s)
Hojo, H., Katayama, H., Takenouchi, T., Shimizu, I., Ueki, A., Nakahara, Y., Nakahara, Y.
Organizer
25th International carbohydrate symposium
Place of Presentation
幕帳メッセ(Tokyo)
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