The clarification and application of mechanism of hypocholesterolemic peptides by peptide array
| Project/Area Number |
20380075
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Gifu University |
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Principal Investigator |
NAGAOKA Satoshi Gifu University, 応用生物科学部, 教授 (50202221)
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| Co-Investigator(Kenkyū-buntansha) |
KATOU Ryuji 名古屋大学, 大学院・工学系研究科, 助教 (50377884)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Keywords | コレステロール / ラクトスタチン / コングリシニン / ペプチドアレイ / 胆汁酸 / コレステロール7α-水酸化酵素 / ミセル / HNF-3α / LXR / HepG2 |
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| Research Abstract |
[1] This experiment was designed to identify the peptides that have bile acid-binding ability from soybean protein to inhibit an intestinal cholesterol absorption by peptide array. We found some bile acid binding peptides in this screening. Furthermore, we evaluated the efficient modification of VAWWMY (cholesterol absorption inhibitory peptide) activity by peptide array. We found PWWWMY and VIWWFK as new peptides with higher activity than VAWWMY.
[2] We studied the mechanism of cholesterol 7α-hydroxylase (CYP7A1) gene transactivation by a luciferase promoter assay. We found that HNF3 or HNF4 were incorporated into the lactostatin induced the transactivation of CYP7A1 gene in HepG2 cells.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Screening of peptides with a high affinity to bile acids using peptide arrays and a computational analysis.2011
Author(s)
Takeshita, T., Okochi, M., Kato, R., Kaga, C.,Tomita, Y., Nagaoka, S., Honda, H.
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Journal Title
J.Biosci.Bioeng. in press
NAID
Related Report
Peer Reviewed
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[Journal Article] The cattle heart protein hydrolysate ameliorates hypercholesterolemia accompanying with the suppression of cholesterol absorption in rats and Caco-2 cells.2009
Author(s)
Nakade, K., Kaneko, H.Oka, T., Ahhmed, A.M., Muguruma, M., Numata, M., Nagaoka, S.
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Journal Title
Biosci.Biotechnol.Biochem. 73
Pages: 607-612
Related Report
Peer Reviewed
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[Journal Article]2009
Author(s)
長岡利
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Journal Title
機能性ペプチドの最新応用技術(コレステロール代謝改善ペプチド)(シーエムシー出版)
Pages: 86-95
Related Report
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