Development of renin inhibitor screening system and structure-function relationship of renin inhibitor(s) from food stuffs
| Project/Area Number |
20380081
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Akita Prefectural Agriculture, Forestry and Fisheries Research Center (2008, 2010)
秋田県総合食品研究所 (2009) |
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Principal Investigator |
TAKAHASHI Saori Akita Prefectural Agriculture, Forestry and Fisheries Research Center, 食品加工研究所, 所長 (10142184)
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| Co-Investigator(Kenkyū-buntansha) |
HORI Kazuyuki 秋田県総合食品研究センター, 企画マーケティング班, 上席研究員 (50181516)
GOTOH Takeshi 秋田大学, 工学資源学部, 教授 (10215494)
YOSHIZAWA Yuko 秋田県立大学, 生物資源科学部, 教授 (20269202)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2010: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2009: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2008: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Keywords | レニン / アンギオテンシン / 血圧調節 / 大豆 / ソヤサポニン / 米 / オレイン酸 / リノール酸 / バキュロウイルス / 阻害物質 / グルクロニドサポニン / アンギオテンシ / 昆虫細胞 / アスパルテックプロテアーゼ / ソヤサポニンI / 活性化酵素 / システインプロテアーゼ |
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| Research Abstract |
We succeeded effective production of recombinant human renin by baculovirus-insect cell expression system. Using recombinant human renin as a target enzyme, we found renin inhibitory activity in soybean and isolated soybean renin inhibitor. The physico-chemical data on the isolated inhibitor were identical with those of soyasaponin I. In addition, we studied the effects of various saponins on recombinant human renin activity and found that so-called glucuronide saponin which glucuronic acid connected to the third position of sapogenol was renin inhibitory saponins. Furthermore, we found the existence of the renin inhibitor to rice and identified the structure with oleic acid and linoleic acid.
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Report
(4 results)
Research Products
(79 results)
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[Journal Article] Reduction of blood pressure by soybean saponins, renin inhibitor from soybean, in spontaneously hypertensive rats.2010
Author(s)
Kazuyuki Hiwatashi, Hitoshi Shirakawa, Kazuyuki Hori, Yumiko Yoshiki, Nao Suzuki, Michio Kimao, Saori Takahashi
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Journal Title
Bioscience, Biotechnology, and Biochemistry 74
Pages: 2310-2312
NAID
Related Report
Peer Reviewed
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[Journal Article] The occurrence of renin inhibitor in rice : Isolation, identification, and, structure and function.2010
Author(s)
Saori Takahashi, Tetsuo Tokiwano, Keishi Hata, Mika Hokari, Nao Suzuki, Ikuko Kodama, Yuko Yoshizawa, Takeshi Gotoh
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Journal Title
Bioscience, Biotechnology, and Biochemistry 74
Pages: 1713-1715
Related Report
Peer Reviewed
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[Journal Article] The occurrence of renin inhibitor in rice : Isolation, identification, and structure and function.2010
Author(s)
Saori Takahashi, Tetsuo Tokiwano, Keishi Hata, Mika Hokarri, Nao Suzuki, Ikuko Kodama, Yuko Yoshizawa, Takeshi Gotoh
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 74
Pages: 1713-1715
Related Report
Peer Reviewed
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[Journal Article] Reduction of blood pressure by soybean saponins, renin inhibitors from soybean, in spontaneously hypertensive rats.2010
Author(s)
Kazuyuki Hiwatashi, Hitoshi Shirakawa, Kazuyuki Hori, Yumiko Yoshiki, Nao Suzuki, Michio Komai, Saori Takahashi
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 74
Pages: 2310-312
NAID
Related Report
Peer Reviewed
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[Presentation] 大豆由来レニン阻害物質2009
Author(s)
高橋砂織, 堀一之, 新保守, 樋渡一之, 後藤猛, 山田清繁
Organizer
2009年度日本農芸化学会大会
Place of Presentation
福岡マリンパレス、福岡市
Year and Date
2009-03-29
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