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Changes of microbial and protozoa community structures influenced by heat disturbance in the boreal forest soil

Research Project

Project/Area Number 20380082
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Forest science
Research InstitutionHokkaido University

Principal Investigator

KASAHARA Yasuhiro  Hokkaido University, 低温科学研究所, 准教授 (20273849)

Co-Investigator(Kenkyū-buntansha) FUKUI Manabu  北海道大学, 低温科学研究所, 教授 (60305414)
SHIMANO Satoshi  宮城教育大学, 環境教育実践研究センター, 准教授 (70355337)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2010: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2008: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Keywords微生物 / 原生生物 / 群集構造 / 環境変動 / 北方森林 / 土壌温暖化
Research Abstract

In the forest soil, warming of 5 degrees for 5 years, the amount of total soil carbon and total nitrogen slightly were decreased. However, the warming disturbance was considered to be little effect on the structure of microbial community in soils. We developed a molecular technique, and established PCR-DGGE analysis to analyze a structure of protist community. For the function analysis of microbial communities in soil, we were able to be established the platform of environmental metaproteomics.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (27 results)

All 2011 2010 2009 2008 2007

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (22 results)

  • [Journal Article] Detection of Effects of a High Trophic Level Predator, Sorex unguiculatus (Soricidae, Mammalia), on a Soil Microbial Community in a Cool Temperate Forest in Hokkaido, Using the ARISA Method.2010

    • Author(s)
      K.Yamamoto, S.D.Ohdachi, Y.Kasahara
    • Journal Title

      Microbes and Environments 25

      Pages: 197-203

    • NAID

      10026643362

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of Effects of a High Trophic Level Predator, Sorex ung uiculatus (Soricidae, Mammalia), on a Soil Microbial Community in a Cool Temperate Forest in Hokkaido, Using the ARISA Met hod2010

    • Author(s)
      Y.Kasahara
    • Journal Title

      Microbes and Environments

      Volume: 25 Pages: 197-203

    • NAID

      10026643362

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Linkage between light microscopic observations and molecular analysis by single-cell PCR for ciliates.2008

    • Author(s)
      S.Shimano, M.Sanbe, Y. Kasahara
    • Journal Title

      Microbes and Environments 23

      Pages: 356-359

    • NAID

      110007005111

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Linkage between light microscopic observations and molecular analysis by single-cell PCR for ciliates2008

    • Author(s)
      S.Shimano, M.Sanbe, Y.Kasahara
    • Journal Title

      Microbes and Environments 23

      Pages: 356-359

    • NAID

      110007005111

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A taxonspecific oligonucleotide primer set for PCR-based detection of soil ciliate.2007

    • Author(s)
      T.Puitika, Y.Kasahara, N.Miyoshi, Y.Sato, S.Shimano
    • Journal Title

      Microbes and Environments 22

      Pages: 78-81

    • NAID

      110006242034

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] 液体培養系および土壌培養系におけるPseudomonas putida F1株の比較プロテオーム解析2011

    • Author(s)
      森本一、桑野晶喜、笠原康裕
    • Organizer
      第5回日本ゲノム微生物学会年会
    • Place of Presentation
      仙台市(東北学院大学)
    • Related Report
      2010 Final Research Report
  • [Presentation] 液体培養系および土壌培養系におけるPseudomonas putidaF1株の比較プロテオーム解析2011

    • Author(s)
      笠原康裕
    • Organizer
      第5回日本ゲノム微生物学会年会
    • Place of Presentation
      仙台市、東北学院大
    • Related Report
      2010 Annual Research Report
  • [Presentation] Proteome analysis of the response of Pseudomonas putida F1 to aromatic hydrocarbon in soil2010

    • Author(s)
      Morimoto H., Kuwano M., Kasahara Y.
    • Organizer
      第26回日本微生物生態学会
    • Place of Presentation
      つくば市(筑波大学)
    • Related Report
      2010 Final Research Report
  • [Presentation] Micro-eukaryote diversity in soil environments of a grass field.2010

    • Author(s)
      Shimano S., Imaizumi Y., Kasahara Y.
    • Organizer
      International Society for Evolutionary Protistology, 18th Meeting (ISEP XVIII)
    • Place of Presentation
      Ishikawa(金沢大学),Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] ゲノム未解析株のプロテオーム解析の評価2010

    • Author(s)
      桑野晶喜、森本一、笠原康裕
    • Organizer
      第4回日本ゲノム微生物学会年会
    • Place of Presentation
      福岡市(九州大学)
    • Related Report
      2010 Final Research Report
  • [Presentation] 間接タンパク質抽出法を用いた土壌メタプロテオミクスの基盤構築2010

    • Author(s)
      森本一、桑野晶喜、笠原康裕
    • Organizer
      第4回日本ゲノム微生物学会年会
    • Place of Presentation
      福岡市(九州大学)
    • Related Report
      2010 Final Research Report
  • [Presentation] 半定量的発現プロテオミクスによる芳香族分解の代謝経路解析2010

    • Author(s)
      森本一、桑野晶喜、笠原康裕
    • Organizer
      第4回日本ゲノム微生物学会年会
    • Place of Presentation
      福岡市(九州大学)
    • Related Report
      2010 Final Research Report
  • [Presentation] Proteome analysis of the response of Pseudomonas putida F1 to aromatic hydrocarbon in soil2010

    • Author(s)
      笠原康裕
    • Organizer
      第26回日本微生物生態学会年会
    • Place of Presentation
      つくば市・筑波大
    • Related Report
      2010 Annual Research Report
  • [Presentation] Micro-eukaryote diversity in soil environments of a grass field.2010

    • Author(s)
      Kasahara Y
    • Organizer
      International Society for Evolutionary Protistology, 18^<th> meeting
    • Place of Presentation
      Kanazawa, Japan
    • Related Report
      2010 Annual Research Report
  • [Presentation] 半定量的発現プロテオミクスによる芳香族分解の代謝経路解析2010

    • Author(s)
      笠原康裕
    • Organizer
      第4回日本ゲノム微生物学会年会
    • Place of Presentation
      福岡市、九州大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] 間接タンパク質抽出法を用いた土壌メタプロテオミクスの基盤構築2010

    • Author(s)
      笠原康裕
    • Organizer
      第4回日本ゲノム微生物学会年会
    • Place of Presentation
      福岡市、九州大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] ゲノム未解析株のプロテオーム解析の評価2010

    • Author(s)
      笠原康裕
    • Organizer
      第4回日本ゲノム微生物学会年会
    • Place of Presentation
      福岡市、九州大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] Biodiversity of micro-eukaryotes in soil environments of a grass field.2009

    • Author(s)
      Shimano S., Imaizumi Y., Kasahara Y.
    • Organizer
      Canadian Society for Ecology and Evolution (CSEE) 2009 meeting.
    • Place of Presentation
      Halifax, Canada
    • Related Report
      2010 Final Research Report
  • [Presentation] 環境変動と土壌微生物群集変動2009

    • Author(s)
      笠原康裕
    • Organizer
      第56回日本生態学会大会
    • Place of Presentation
      盛岡市(岩手県立大学)
    • Related Report
      2010 Final Research Report
  • [Presentation] 大腸菌のVBNC 状態関与遺伝子のゲノムワイドスクリーニング2009

    • Author(s)
      鶴谷安弘、森浩禎、笠原康裕
    • Organizer
      第3回ゲノム微生物学会年会
    • Place of Presentation
      東京(中央大学)
    • Related Report
      2010 Final Research Report
  • [Presentation] Biodiversity of micro-eukaryotes in soil environments of a grass field2009

    • Author(s)
      Kasahara Y
    • Organizer
      Canadian Society for Ecology and Evolution(CSEE)2009 meeting
    • Place of Presentation
      Halifax, Canada
    • Related Report
      2009 Annual Research Report
  • [Presentation] 環境変動と土壌微生物群集変動2009

    • Author(s)
      笠原康裕
    • Organizer
      第56回日本生態学会大会
    • Place of Presentation
      岩手、盛岡県立大
    • Related Report
      2008 Annual Research Report
  • [Presentation] 土壌微生物群集に対する高次捕食者トガリネズミの効果(予報)2008

    • Author(s)
      山本佳奈、大舘智志、笠原康裕
    • Organizer
      第24回日本微生物生態学会
    • Place of Presentation
      札幌市(北海道北海道大学)
    • Related Report
      2010 Final Research Report
  • [Presentation] Ciliate community analysis of agricultural soil based on SSU rDNA with new taxon-specific primer.2008

    • Author(s)
      Shimano S., Sanbe M., Puitika T., Kasahara Y.
    • Organizer
      The International Society of Protistologists (ISOP), 59th Annual Meeting, and the International Society for Evolutionary Protistology, 17th Meeting (ISEP XVII).
    • Place of Presentation
      Halifax, Canada
    • Related Report
      2010 Final Research Report
  • [Presentation] シングルセルPCR法による繊毛虫類の塩基配列解析2008

    • Author(s)
      高叡瑞、明石典之、熊谷朋子、三部光男、今泉友希、外薗香菜、笠原康裕、島野智之
    • Organizer
      日本土壌動物学会第31回記念大会
    • Place of Presentation
      那覇市(琉球大学)
    • Related Report
      2010 Final Research Report
  • [Presentation] シングルセルPCR法による繊毛虫類の塩基配列解析2008

    • Author(s)
      笠原康裕・島野智之
    • Organizer
      日本土壌動物学会
    • Place of Presentation
      琉球大学
    • Related Report
      2008 Annual Research Report
  • [Presentation] Ciliate community analysis of agricultural soil based on SSU rDNA with new taxon-specific primer2008

    • Author(s)
      Shimano S., Kasahara Y
    • Organizer
      59th Annual Meeting, and the International Society for Evolutionary Protistology, 17th Meeting
    • Place of Presentation
      Halifax, Canada
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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