Genetic analysis genes that regulates male flower abundance of Cryptomeria japonic

• Project/Area Number: 20380096
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Forest science
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: IHARA Tokuko 独立行政法人森林総合研究所, 森林遺伝研究領域, 主任研究員 (40353594)
• Co-Investigator(Kenkyū-buntansha): IWATA Hiroyoshi 東京大学, 農学生命科学研究科, 准教授 (00355489) ; TSUBOMURA Miyoko 独立行政法人森林総合研究所, 林木育種センター, 研究員 (70415040) ; WATANABE Atsushi 独立行政法人森林総合研究所, 林木育種センター, 室長 (10360471) ; 河崎 久男 独立行政法人森林総合研究所, 林木育種センター, 育種第二課長 (50370846)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000); Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2008: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
• Keywords: スギ雄花 / 遺伝解析 / QTL / アソシーエーション解析 / 花成 / スギ / 雄花着生量 / QTL解析 / DNAマーカー

Research Abstract

Using cross-family derived from CR46, which bears abundant male strobili every year, association between DNA polymorphism and male strobili abundance was investigated. Stable and strong quantitative trait loci (QTLs) were detected. Association between genotype of markers in the detected QTL region and male flower abundance were further investigated using 150 plus-trees selected from Kanto region. Significant association was detected for one locus, and genomic clone including this locus has been isolated.

Research Products

• [Journal Article] Identification of QTLs associated with male strobilus abundance in Cryptomeria japonica. (2013)
  • Author(s): Ujino-Ihara T, Iwata H, Taguchi Y and Tsumura Y
  • Journal Title: TREE GENETICS & GENOMES Volume: 8 Pages: 1319-1329
  • Peer Reviewed
• [Journal Article] The construction of a high-density linkage map for identifying SNP markers that are tightly linked to a nuclear-recessive major gene for male sterility in Cryptomeria japonica D. Don. (2013)
  • Author(s): Moriguchi Y, Ujino-Ihara T
  • Journal Title: BMC Genomics Volume: 13:95
  • Peer Reviewed
• [Journal Article] Identification of QTLs associated with male strobilus abundance in Cryptomeria japonica. (2012)
  • Author(s): Ujino-Ihara T, Taguchi Y, Moriguchi Y, Tsumura Y
  • Journal Title: TREE GENETICS & GENOMES Volume: 8 Issue: 6 Pages: 1319-1329
  • DOI: 10.1007/s11295-012-0518-3
  • Peer Reviewed
• [Journal Article] The construction of a high-density linkage map for identifying SNP markers that are tightly linked to a nuclear-recessive major gene for male sterility in Cryptomeria japonica D.Don (2012)
  • Author(s): 森口喜成、伊原徳子、内山憲太郎、二村典宏、斎藤真己、上野真義、松本麻子、谷尚樹、平英彰、篠原健司、津村義彦
  • Journal Title: BMC Genomics Volume: 13 Issue: 1
  • DOI: 10.1186/1471-2164-13-95
  • Peer Reviewed
• [Journal Article] Prospects for genomic selection in conifer breeding: a simulation study of Cryptomeria japonica (2011)
  • Author(s): Iwata H, Hayashi T, Tsumura Y
  • Journal Title: Tree Genetics & Genomes. Tree Genetics and Genomes Volume: 7 Pages: 747-758
  • Peer Reviewed
• [Journal Article] Prospects for genomic selection in conifer breeding : a simulation study of Cryptomeria japonica. (2011)
  • Author(s): Iwata H, Hayashi T, Tsumura Y
  • Journal Title: Tree Genetics & Genomes. On-line first.
  • Peer Reviewed
• [Journal Article] An efficient method for developing SNP markers based on EST data combined with high resolution melting (HRM) analysis (2010)
  • Author(s): Ujino-Ihara T, Taguchi Y, Moriguchi Y, Tsumura Y
  • Journal Title: BMC Research Notes Volume: 3:51
  • Peer Reviewed
• [Journal Article] An efficient method for developing SNP markers based on EST data combined with high resolution melting (HRM) analysis. (2010)
  • Author(s): Ujino-Ihara T, Taguchi Y, Moriguchi Y, Tsumura Y.
  • Journal Title: BMC Research Notes. 3 Pages: 51-51
  • Peer Reviewed
• [Journal Article] An efficient method for developing SNP markers based on EST data combined with high resolution melting(HRM)analysis (2010)
  • Author(s): 伊原徳子、田口由利子、森口喜成、津村義彦
  • Journal Title: BMC Research Notes 3
  • Peer Reviewed
• [Journal Article] Screening for genes specific to coniferous species (2008)
  • Author(s): Ujino-Ihara T, Tsumura Y
  • Journal Title: Tree Physiology Volume: 28 Pages: 1325-1330
  • Peer Reviewed
• [Journal Article] Screening for genes specific to coniferous species. (2008)
  • Author(s): Ujino-Ihara T, Tsumura Y.
  • Journal Title: Tree Physiology 28 Pages: 1325-1330
  • Peer Reviewed
• [Journal Article] Screening for genes specific to coniferous species (2008)
  • Author(s): Ujino-Ihara T, Tsumura Y
  • Journal Title: Tree Physiology 28 Pages: 1325-1330
  • Peer Reviewed
• [Presentation] スギ雄花着生量に関与する遺伝子座の遺伝解析 (2012)
  • Author(s): 伊原 徳子、 森口 喜成、 内山 憲太郎、 坪 村 美代子、 岩田 洋佳、 渡辺 敦史、 津村 義彦
  • Organizer: 日本森林学会第123回大会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2012-03-27
• [Presentation] スギ雄花着生量に関与する遺伝子座の遺伝解析 (2012)
  • Author(s): 伊原徳子、森口喜成、内山憲太郎、坪村美代子、岩田洋佳、渡辺敦史、津村義彦
  • Organizer: 日本森林学会第123回大会
  • Place of Presentation: 宇都宮大学(宇都宮)
  • Year and Date: 2012-03-27
• [Presentation] ゲノミックセレクションが林木育種に変革をもたらすか? (2010)
  • Author(s): 岩田洋佳、林武司、津村義彦
  • Organizer: 日本森林学会第121回大会
  • Place of Presentation: 筑波大学(発表予定)
  • Year and Date: 2010-04-04
• [Presentation] Prospect for genomic selection in forest tree breeding (2009)
  • Author(s): Iwata H, Hayashi T, Tsumura Y
  • Organizer: Eucarpia XIV meeting of the Biometrics in Plant Breeding Section
  • Place of Presentation: Dundee, UK
  • Year and Date: 2009-09-02
• [Presentation] Prospect for genomic selection in forest tree breeding. (2009)
  • Author(s): Iwata H, Hayashi T, Tsumura Y.
  • Organizer: Eucarpia XIV meeting of the Biometrics in Plant Breeding Section.
  • Place of Presentation: Dundee, UK.
  • Year and Date: 2009-09-02
• [Presentation] スギ雄花着生量を制御する遺伝 子を探る.遺伝子型から表現型へ・ゲノム育 種の林木における可能性 (2009)
  • Author(s): 伊原徳子
  • Organizer: 第120回日本森林学会大会
  • Place of Presentation: 京都大学
  • Year and Date: 2009-03-27
• [Presentation] スギ雄花着生量を制御する遺伝子を探る～遺伝子型から表現型へ・ゲノム育種の林木における可能性～ (2009)
  • Author(s): 伊原徳子
  • Organizer: 第120回日本森林学会大会
  • Year and Date: 2009-03-27
• [Presentation] Prospects for genomic selection of forest tree breeding : Simulation study for genetic improvement of conifer, Cryptomeria japonica (2009)
  • Author(s): 岩田洋佳、林武司、津村義彦
  • Organizer: Eucarpia Biometrics
  • Place of Presentation: Dundee, UK
• [Presentation] スギ雄花着生量を制御する遺伝子を探る (2009)
  • Author(s): 伊原徳子
  • Organizer: 第120回日本森林学会大会
  • Place of Presentation: 京都大学
• [Remarks]
  • URL: http://www.ffpri.affrc.go.jp/labs/cjgenome/indexj.html
• [Remarks] スギゲノムデータベース
  • URL: http://www.ffpri.affrc.go.jp/labs/cjgenome/indexj.html