Study on shrimp immune system against microbial pathogens

• Project/Area Number: 20380109
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: HIRONO Ikuo 東京海洋大学, 海洋科学技術研究科, 教授 (00270926)
• Co-Investigator(Kenkyū-buntansha): KONDO Hidehiro 東京海洋大学, 海洋科学技術研究科, 准教授 (20314635)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000); Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
• Keywords: クルマエビ / 免疫 / 生体防御 / RNA干渉 / 抗菌タンパク質 / 血球凝集因子

Research Abstract

We identified that TGase is an important component of the shrimp immune response and is involved in the regulation of some immune-related genes particularly antimicrobial peptides. We also found the potential of non-specific dsRNA injection to shrimp to elicit antimicrobial response in shrimp. We developed the microarray of kuruma shrimp based on the EST analysis for further study of kuruma shrimp immune gene network.

Research Products

• [Journal Article] Uncovering the Mechanisms of Shrimp Innate Immune Response by RNA Interference (2011)
  • Author(s): Hirono I, Fagutao FF, Kondo H, Aoki T
  • Journal Title: Mar Biotechnol Volume: 13 Issue: 4 Pages: 622-628
  • DOI: 10.1007/s10126-010-9292-0
• [Journal Article] Molecular characterization and expression analysis of heat shock proteins 40, 70 and 90 from kuruma shrimp Marsupenaeus japonicus (2011)
  • Author(s): Danwattananusorn T, Fagutao FF, Shitara A, Kondo H, A oki T, Nozaki R, Hirono I
  • Journal Title: Fish Sci Volume: 77 Issue: 6 Pages: 929-937
  • DOI: 10.1007/s12562-011-0394-z
  • NAID: 10029654416
• [Journal Article] Uncovering the Mechanisms of Shrimp Innate Immune Response by RNA Interference. (2011)
  • Author(s): Hirono I, Fagutao FF, Kondo H, Aoki T.
  • Journal Title: Mar Biotechnol in press
• [Journal Article] PmPPAE2, a new class of crustacean prophenoloxidase (proPO)-activating enzyme and its role in PO activation. (2011)
  • Author(s): Charoensapsri W, Amparyup P, Hirono I, Aoki T, Tassanakajon A.
  • Journal Title: Dev Comp Immunol. 35 Pages: 115-124
• [Journal Article] PmPPAE2, a new class of crustacean prophenoloxidase (proPO)-activatingenzyme and its role in PO activation. (2011)
  • Author(s): Charoensapsri W, Amparyup P, Hirono 1, Aoki T, Tassanakajon A.
  • Journal Title: Dev Comp Immunol Volume: 35 Pages: 115-124
  • Peer Reviewed
• [Journal Article] A cDNA microarray approach for analyzing transcriptional changes in Penaeus monodon after infection by pathogens. (2011)
  • Author(s): Pongsomboon S, Tang S, Boonda S, Aoki T, Hirono 1, Tassanakajon A
  • Journal Title: Fish Shellfish Immunol Volume: 30
  • Peer Reviewed
• [Journal Article] Uncovering the Mechanisms of Shrimp Innate Immune Response by RNA Interference. (2011)
  • Author(s): Hirono I, Fagutao FF, Kondo H, Aoki T.
  • Journal Title: Mar Biotechnol Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Involvement of WSSV-shrimp homologs in WSSV infectivity in kuruma shrimp ; Marsupenaeus japonicus. (2011)
  • Author(s): Dang LT, Koyama T, Shitara A, Kondo H, Aoki T, Hirono I.
  • Journal Title: Antiviral Res Volume: 88 Pages: 217-226
  • Peer Reviewed
• [Journal Article] Involvement of WSSV-shrimp homologs in WSSV infectivity in kuruma shrimp ; Marsupenaeus japonicus (2010)
  • Author(s): Dang LT, Koyama T, Shitara A, Kondo H, Aoki T, Hirono I
  • Journal Title: Antiviral Res Volume: 88 Issue: 2 Pages: 217-226
  • DOI: 10.1016/j.antiviral.2010.08.017
• [Journal Article] Molecular characterization and expression analysis of a c-type and two novel muramidase-deficient i-type lysozymes from Penaeus monodon (2010)
  • Author(s): Supungul P, Rimphanitchayakit V, Aoki T, Hirono I, Tassanakajon A
  • Journal Title: Fish Shellfish Immunol Volume: 28 Issue: 3 Pages: 490-498
  • DOI: 10.1016/j.fsi.2010.01.003
• [Journal Article] Hyper-expansion of large DNA segments in the genome of kuruma shrimp, Marsupenaeus japonicus (2010)
  • Author(s): Koyama T, Asakawa S, Katagiri T, Shimizu A, Fagutao FF, Mavichak R, Santos MD, Fuji K, Sakamoto T, Kitakado T, Kondo H, Shimizu N, Aoki T, Hirono I
  • Journal Title: BMC Genomics Volume: 11 Issue: 1 Pages: 141-141
  • DOI: 10.1186/1471-2164-11-141
  • Peer Reviewed
• [Journal Article] The Marsupenaeus japonicus voltage-dependent anion channel(MjVDAC) protein is involved in WSSV pathogenesis (2010)
  • Author(s): Wang HC, Kondo H, Hirono I, Aoki T
  • Journal Title: Fish Shellfish Immunol Volume: 29 Issue: 1 Pages: 94-103
  • DOI: 10.1016/j.fsi.2010.02.020
• [Journal Article] A cDNA microarray approach for analyzing transcriptional changes in Penaeus monodon after infection by pathogens (2010)
  • Author(s): Pongsomboon S, Tang S, Boonda S, Aoki T, Hirono I, Tassanakajon A
  • Journal Title: Fish Shellfish Immunol Volume: 30 Issue: 1 Pages: 439-446
  • DOI: 10.1016/j.fsi.2010.10.015
• [Journal Article] A cDNA microarray approach for analyzing transcriptional changes in Penaeus monodon after infection by pathogens. (2010)
  • Author(s): Pongsomboon S, Tang S, Boonda S, Aoki T, Hirono I, Tassanakajon A.
  • Journal Title: Fish Shellfish Immunol. 30 Pages: 439-446
• [Journal Article] The Marsupenaeus japonicus voltage-dependent anion channel (MjVDAC) protein is involved in WSSV pathogenesis. (2010)
  • Author(s): Wang HC, Kondo H, Hirono I, Aoki T.
  • Journal Title: Fish Shellfish Immunol 29 Pages: 94-103
• [Journal Article] Hyper-expansion of large DNA segments in the genome of kuruma shrimp, Marsupenaeus japonicus. (2010)
  • Author(s): Koyama T, Asakawa S, Katagiri T, Shimizu A, Fagutao FF, Mavichak R, Santos MD, Fuji K, Sakamoto T, Kitakado T, Kondo H, Shimizu N, Aoki T, Hirono I.
  • Journal Title: BMC Genomics 11 Pages: 141-141
• [Journal Article] Molecular characterization and expression analysis of a c-type and two novel muramidase-deficient i-type lysozymes from Penaeus monodon. (2010)
  • Author(s): Supungul P, Rimphanitchayakit V, Aoki T, Hirono I, Tassanakajon A.
  • Journal Title: Fish Shellfish Immunol 28 Pages: 490-498
• [Journal Article] Involvement of WSSV-shrimp homologs in WSSV infectivity in kuruma shrimp ; Marsupenaeus japonicus. (2010)
  • Author(s): Dang LT, Koyama T, Shitara A, Kondo H, Aoki T, Hirono I.
  • Journal Title: Antiviral Res 88 Pages: 217-226
• [Journal Article] Hyper-expansion of large DNA segments in the genome of kuruma shrimp, Marsupenaeus japonicus. (2010)
  • Author(s): Koyama T, Asakawa S, Katagiri T, Shimizu A, Fagutao FF, Mavichak R, Santos MD, Fuji K, Sakamoto T, Kitakado T, Kondo H, Shimizu N, Aoki T, Hirono I.
  • Journal Title: BMC Genomics Volume: 11 Pages: 141-141
  • Peer Reviewed
• [Journal Article] Molecular characterization and expression analysis of a c-type and twonovel muramidase-deficient i-type lysozymes from Penaeus monodon. (2010)
  • Author(s): Supungul P, Rimphanitchayakit V, Aoki T, Hirono I, Tassanakaj on A.
  • Journal Title: Fish Shellfish Immunol Volume: 28 Pages: 490-498
  • Peer Reviewed
• [Journal Article] The Marsupenaeus japonicus voltage-dependent anion channel (MjVDAC)protein is involved in WSSV pathogenesis. (2010)
  • Author(s): Wang HC, Kondo H, Hirono I, Aoki T.
  • Journal Title: Fish Shellfish Immunol Volume: 29 Pages: 94-103
  • Peer Reviewed
• [Journal Article] Increased bacterial load in shrimp hemolymph in the absence of prophenoloxidase (2009)
  • Author(s): Fagutao FF, Koyama T, Kaizu A, Saito-Taki T, Kondo H, Aoki T, Hirono I
  • Journal Title: FEBS J Volume: 276 Issue: 18 Pages: 5298-5306
  • DOI: 10.1111/j.1742-4658.2009.07225.x
• [Journal Article] Differential gene expression in black tiger shrimp, Penaeus monodon, following administration of oxytetracycline and oxolinic acid (2009)
  • Author(s): Fagutao FF, Yasuike M, Santos MD, Ruangpan L, Sangrunggruang K, Tassanakajon A, Takahashi Y, Ueno R, Kondo H, Hirono I, Aoki T
  • Journal Title: Dev Comp Immunol Volume: 33 Issue: 10 Pages: 1088-1092
  • DOI: 10.1016/j.dci.2009.05.010
• [Journal Article] Increased bacterial load in shrimp hemolymph in the absence of prophenoloxidase. (2009)
  • Author(s): Fagutao FF, Koyama T, Kaizu A, Saito-Taki T, Kondo H, Aoki T, Hirono I.
  • Journal Title: FEBS J. 276 Pages: 5298-5306
• [Journal Article] Differential gene expression in black tiger shrimp, Penaeus monodon, following administration of oxytetracycline and oxolinic acid. (2009)
  • Author(s): Fagutao FF, Yasuike M, Santos MD, Ruangpan L, Sangrunggruang K, Tassanakajon A, Takahashi Y, Ueno R, Kondo H, Hirono I, Aoki T.
  • Journal Title: Dev Comp Immunol. 33 Pages: 1088-1092
• [Journal Article] Differential gene expression in black tiger shrimp, Penaeusmonodon, following administration of oxytetracycline and oxolinic acid (2009)
  • Author(s): Fagutao FF, Yasuike M, Santos MD, Ruangpan L, Sangrunggruang K, Tassanakajon A, Takahashi Y, Ueno R, Kondo H, Hirono I, Aoki T.
  • Journal Title: Developmental and Comparative Immunology 33 Pages: 1088-1092
  • Peer Reviewed
• [Journal Article] Increased bacterial load in shrimp hemolymph in the absence of prophenoloxidase (2009)
  • Author(s): Fagutao FF, Koyama T, Kaizu A, Saito-Taki T, Kondo H, Aoki T, Hirono I.
  • Journal Title: FEBS J. 276 Pages: 5298-5306
  • Peer Reviewed
• [Journal Article] Molecular cloning, characterization and expression analysis of a chymotrypsin-like serine protease from kuruma shrimp Marsupenaeus japonicus (2009)
  • Author(s): Danwattananusorn T, Kondo H, Aoki T, Hirono I.
  • Journal Title: Fisheries Science 75 Pages: 1231-1238
  • NAID: 10025406919
  • Peer Reviewed
• [Journal Article] Gene expression profile of haemocytes of kuruma shrimp, Marsupenaeus japonicus following peptidoglycan stimulation (2008)
  • Author(s): Fagutao FF, Yasuike M, Caipang CM, Kondo H, Hirono I, Takahashi Y, Aoki T
  • Journal Title: Mar Biotechnol Volume: 10 Issue: 6 Pages: 731-740
  • DOI: 10.1007/s10126-008-9110-0
• [Journal Article] Gene expression profile of haemocytes of kuruma shrimp, Marsupenaeus japonicus following peptidoglycan stimulation. (2008)
  • Author(s): Fagutao FF, Yasuike M, Caipang CM, Kondo H, Hirono I, Takahashi Y, Aoki T.
  • Journal Title: Mar Biotechnol 10 Pages: 731-740
• [Journal Article] Differentially expressed genes in Penaeus monodon hemocytes following infection with yellow head virus. (2008)
  • Author(s): Pongsomboon S, Tang S, Boonda S, Aoki T, Hirono I, Yasuike M, Tassanakajon A.
  • Journal Title: BMB Rep. 41 Pages: 670-677
• [Journal Article] A peroxiredoxin from Kuruma shrimp, Marsupenaeus japonicus inhibited by peptidoglycan. (2008)
  • Author(s): Maningas MBB, Koyama T, Kondo H, Hirono I, Aoki T.
  • Journal Title: Dev Comp Immunol. 32 Pages: 198-203
• [Journal Article] Essential function of transglutaminase and clotting protein in shrimp immunity. (2008)
  • Author(s): Maningas MBB, Kondo H, Hirono I, Taki T, Aoki T.
  • Journal Title: Mol.Immunol. 45 Pages: 1269-1275
• [Journal Article] A peroxiredoxin from Kuruma shrimp, Marsupenaeus japonicusinhibited by peptidoglycan. (2008)
  • Author(s): Maningas MBB, Koyama T, Kondo H, Hirono I, Aoki T.
  • Journal Title: Developmental and Comparative Immunology 32 Pages: 198-203
  • Peer Reviewed
• [Journal Article] Essential function of transglutaminase and clotting protein in shrimp immunity. (2008)
  • Author(s): Maningas MBB, Kondo H, Hirono I, Taki T, Aoki T.
  • Journal Title: Molecular Immunology 45 Pages: 1269-1275
  • Peer Reviewed
• [Journal Article] Molecular cloning, genomic organization and recombinantexpression of a crustin-like antimicrobial peptide from black tigershrimp Penaeus monodon. (2008)
  • Author(s): Amparyup P, Kondo H, Hirono I, Aoki T, Tassanakajon A.
  • Journal Title: Molecular Immunology 45 Pages: 1085-1093
  • Peer Reviewed
• [Presentation] RNA干渉法を用いたクルマエビペナエジン様遺伝子の機能解析 (2011)
  • Author(s): 古宮謙太・海津彰弘・近藤秀裕・廣野育生
  • Organizer: 平成23年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京都)
  • Year and Date: 2011-03-28
• [Presentation] クルマエビゲノム中に新たに見つかったWSSV類似遺伝子の構造および発現解析 (2011)
  • Author(s): 設楽愛子・山田裕恵・近藤秀裕・青木宙・廣野育生
  • Organizer: 平成23年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京都)
  • Year and Date: 2011-03-28
• [Presentation] RNA干渉法を用いたクルマエビペナエジン様遺伝子の機能解析 (2011)
  • Author(s): 古宮謙太・海津彰弘・近藤秀裕・廣野育生
  • Organizer: 平成23年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
• [Presentation] クルマエビゲノム配列中に新たに発見されたWSSV類似配列の発現解析 (2010)
  • Author(s): 山田裕恵・設楽愛子・海津彰弘・近藤秀裕・廣野育生・Mary Beth B.Maningas
  • Organizer: 平成22年度日本水産学会秋期大会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2010-09-23
• [Presentation] クルマエビゲノム中に存在する遺伝子ユニットの解析 (2010)
  • Author(s): 設楽愛子・小山喬・近藤秀裕・青木宙・廣野育生
  • Organizer: 平成22年度日本水産学会秋期大会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2010-09-23
• [Presentation] クルマエビのペナエジン様cDNA構造およびRNA干渉による機能解析 (2010)
  • Author(s): 古宮謙太・海津彰弘・近藤秀裕・廣野育生
  • Organizer: 平成22年度日本水産学会秋期大会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2010-09-22
• [Presentation] クルマエビのペナエジン様 cDNA 構造およびRNA干渉による機能解 (2010)
  • Author(s): 古宮謙太・海津彰弘・近藤秀裕・廣野育生
  • Organizer: 平成22年度日本水産学会秋期大会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2010-09-22
• [Presentation] クルマエビリゾチームの生体内での役割について (2010)
  • Author(s): 海津彰弘, 近藤秀裕, 青木宙, 廣野育生
  • Organizer: 第13回マリンバイオテクノロジー学会
  • Place of Presentation: 広島大学(広島県)
  • Year and Date: 2010-05-29
• [Presentation] クルマエビゲノム配列に存在するWSSV類似遺伝子の発現解析 (2010)
  • Author(s): 設楽愛子、小山喬、近藤秀裕、青木宙、廣野育生
  • Organizer: 第13回マリンバイオテクノロジー学会
  • Place of Presentation: 広島大学(広島県)
  • Year and Date: 2010-05-29
• [Presentation] Molecular biology of shrimp (2010)
  • Author(s): Ikuo Hirono
  • Organizer: 2010 Symposium on Biotechnology Development in Taiwan
  • Place of Presentation: National Cheng Kung University(台南市、台湾)
  • Year and Date: 2010-04-20
• [Presentation] Molecular biology of shrimp (2010)
  • Author(s): Ikuo Hirono
  • Organizer: 2010 Symposium on Biotechnology Development in 12010 *4 A 20 HTaiwan
  • Place of Presentation: National Cheng Kung University (台湾市、台湾)
  • Year and Date: 2010-04-20
• [Presentation] Molecular immunology on kuruma shrimp (2010)
  • Author(s): I Hirono, T Koyama, A Kaizu, A Shitara, MBB Maningas, FF Fagtao, H Yamada, K Komiya, T Aoki, H Kondo
  • Organizer: Marsupenaeus japonicus, Sixth International Symposium on Aquatic Animal Health
  • Place of Presentation: タンパマリオットウオーターサイドホテル(タンパ、フロリダ)
• [Presentation] Shrimp lysozyme is important to regulate gram-negative bacteria in haemolymph (2010)
  • Author(s): A Kaizu, H Kondo, T Aoki and I Hirono
  • Organizer: Sixth International Symposium on Aquatic Animal Health
  • Place of Presentation: タンパマリオットウオーターサイドホテル(タンパ、フロリダ)
• [Presentation] Molecular immunology on kuruma shrimp, Marsupenaeus japonicus (2010)
  • Author(s): I Hirono, T Koyama, A Kaizu, A Shitara, MBB Maningas, FF Fagtao, H Yamada, K Komiya, T Aoki, H Kondo
  • Organizer: Sixth International Symposium on Aquatic Animal Health
  • Place of Presentation: タンパ、フロリダ
• [Presentation] クルマエビリゾチームの生体内での役割について (2010)
  • Author(s): 海津彰弘, 近藤秀裕, 青木宙, 廣野育生
  • Organizer: 第13回マリンバイオテクノロジー学会
  • Place of Presentation: 広島大学
• [Presentation] Characterization of the genes homologous to WSSV genome in the genomic DNA of kuruma shrimp, Marsupenaeus japonicus (2010)
  • Author(s): A Shitara, H Yamada, T Koyama, Maningas MBB, H kondo, TAoki, I Hirono
  • Organizer: Sixth International Symposium on Aquatic Animal Health
  • Place of Presentation: タンパマリオットウオーターサイドホテル(タンパ、フロリダ)
• [Presentation] Effect of low water temperature on viral infection and replication of whitespot syndrome virus (WSSV) in kuruma shrimp, Marsupenaeus japonicus (2010)
  • Author(s): LT Dang, A Shitara, A Kaizu, H Kondo, T Aoki , I Hirono
  • Organizer: Sixth International Symposium on Aquatic Animal Health
  • Place of Presentation: タンパマリオットウオーターサイドホテル(タンパ、フロリダ)
• [Presentation] Molecular immunology on kuruma shrimp, Marsupenaeus japonicus (2010)
  • Author(s): I Hirono, T Koyama, A Kaizu, A Shitara, MBB Maningas, FF Fagtao, H Yamada, K Komiya, TAoki, H Kondo
  • Organizer: Sixth International Symposium on Aquatic Animal Health
  • Place of Presentation: タンパマリオットウオーターサイドホテル(タンパ、フロリダ)
• [Presentation] クルマエビゲノム高頻度巨大反復配列中に存在した新規Heat Shock Protein70の解析 (2010)
  • Author(s): 設楽愛子・小山喬・近藤秀裕・青木宙・廣野育生
  • Organizer: 第33回分子生物学会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
• [Presentation] Transglutaminase may regulate immune-related genes in shrimp (2009)
  • Author(s): Fernand Fagutao, Mary Beth Maningas, Hidehiro Kondo, Takashi Aoki, Ikuo Hirono
  • Organizer: 平成21年度日本水産学会秋季大会
  • Place of Presentation: 盛岡
  • Year and Date: 2009-09-30
• [Presentation] クルマエビリゾチームの局在と活性について (2009)
  • Author(s): 海津彰弘・近藤秀裕・青木宙・廣野育生
  • Organizer: 平成21年度日本魚病学会
  • Place of Presentation: 東北大学農学部
  • Year and Date: 2009-09-28
• [Presentation] ABSENCE OF PROPHENOLOXIDASE CAUSES INCREASE IN BACTERIAL LOAD IN SHRIMP HAEMOLYMPH (2009)
  • Author(s): F.F.Fagutao, T.Koyama, A.Kaizu, H.Kondo, T.Aoki, I.Hirono
  • Organizer: 第11回国際比較免疫学会
  • Place of Presentation: プラハ (チェコ)
  • Year and Date: 2009-06-30
• [Presentation] Molecular Immunology and Genomics of Shrimp, ABIC 2009 (2009)
  • Author(s): Ikuo Hirono
  • Organizer: Agricultural Biotechnology for Better Living and a Clean Environment
  • Place of Presentation: Queen Sirikit National Convention Center, Bangkok, Thailand
• [Presentation] Molecular cloning, characterization, and expression analysis of chymotrypsin-like serine proteinase from kuruma shrimp Marsupenaeus japonicus (2009)
  • Author(s): Threechada Danwattananusorn, Hidehiro Kondo, Takashi Aoki, Ikuo Hirono
  • Organizer: 第10回国際養殖遺伝学会議
  • Place of Presentation: バンコク (タイ)
• [Presentation] 二本鎖RNAがクルマエビの生体防御に与える影響について (2008)
  • Author(s): 海津彰弘, 近藤秀裕, 青木宙, 廣野育生
  • Organizer: 第11回マリンバイオテクノロジー学会大会
  • Place of Presentation: 京都大学
• [Presentation] Prophenoloxidase knockdown causes increase in bacterial load in kuruma shrimp haemolymph. (2008)
  • Author(s): Fernand Fagutao, Takashi Koyama, Hidehiro Kondo, Takashi Aoki, Ikuo Hirono
  • Organizer: The 7th Symposium on Diseases in AsianAquaculture
  • Place of Presentation: 台北(台湾)
• [Presentation] Analysis of gene expression profile in Marsupenaeus japonicus after injection with dsRNA (2008)
  • Author(s): Kaizu, H. Kondo, T. Aoki, I. Hirono
  • Organizer: Fifth International Symposium of The Japanese Society for Fish Pathology
  • Place of Presentation: 東京大学農学部
• [Presentation] Knockdown of prophenoloxidase gene leads to increase in bacterial load in haemolymph of kuruma shrimp, Marsupenaeus japonicus (2008)
  • Author(s): Fernand FAGUTAO, Takashi KOYAMA, Hidehiro KONDO, Takashi AOKI, Ikuo HIRONO
  • Organizer: 5th World Fisheries Congress
  • Place of Presentation: 横浜市