| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2008: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Research Abstract |
To develop and establish the direct stock assessment by the Egg Production Method in chub mackerel, following studies have been performed. 1) Development of the new clearing solution for identification of the nucleus in the formalin-preserved oocyte, 2) Development of the simple method to identify the postovulatory follicles by using PAS staining, and estimation of the spawning frequency in the wild stock, 3) Analysis of the cell division cycles in oogonia and oocytes, and mode of the determination of total fecundity, and 4) Evaluation of spawning in individual female fish based on the mitochondrial DNA analysis.
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