| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2008: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Research Abstract |
The molecular mechanism of the functions of rhamnose-binding lectins (RBLs) from fish eggs was revealed. RBL played a role in biodefense by binding to a microsporidian as well as bacteria via glycolipids and lipopolysaccharides. The precise structures of three RBLs, CSL1, CSL2 and CSL3, from chum salmon were analyzed by using a novel chromatographic technique and X-ray crystallography. CSLs had the opsonic effect on the macrophage cell line from rainbow trout and induced the production of proinflammatory cytokines and reactive oxygen species. They were applied to reduce the infiltration of an opportunistic bacterium and induce the apoptotic death of human colon adenocarcinoma cell lines. Furthermore, the expression system for recombinant RBLs was constructed.
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