Characterization of molecular mechanisms of bovine fat accumulation by global gene expression analysis

• Project/Area Number: 20380149
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Zootechnical science/Grassland science
• Research Institution: Shinshu University
• Principal Investigator: ROH Sanggun Shinshu University, 大学院・農学研究科, 准教授 (90322130)
• Co-Investigator(Kenkyū-buntansha): GOTOH Takfumi 九州大学, 農学研究院, 准教授 (70294907) ; CHIKUNI Koichi 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所・研究チーム, チーム長 (40355061) ; HIRAMATSU Kohzi 信州大学, 農学部, 教授 (80238386)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2010: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2008: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: ウシ / 脂肪細胞 / 遺伝子解析 / Chemerin / Adipogenin / 脂肪組織 / 網羅的解析

Research Abstract

This study was to investigate the molecular mechanism of fat accumulation and adipogenesis by DNA chip using adipose tissues from 10- and 24-month-old Japanese Black Cattle. Several candidate genes were separated and identified from global gene expression analysis. Adipogenin, chemerin and mPARM-1 genes were confirmed to relate to lipid metabolism and adipocyte differentiation in bovine adipocytes. These results suggested that several genes were involved with adipogenesis and fat accumulation in bovine.

Research Products

• [Journal Article] Cellular conditions for intramuscular fat deposition in Japanese Black and Holstein steers. (2011)
  • Author(s): Albrecht, E., T.Gotoh, F.Ebara, J.X.Xu, T.Viergutz, G.Nuernberg, S.Maak, J.Wegner.
  • Journal Title: Meat Science in press.
  • Peer Reviewed
• [Journal Article] Gene expression and hormonal regulation of adiponectin and its receptors in bovine mammary gland and mammary epithelial cells. (2011)
  • Author(s): Ohtani Y, Yonezawa T, Song SH, Takahashi T, Ardiyanti A, Sato K, Hagino A, Roh SG, Katoh K.
  • Journal Title: Animal Science Journal. 82 Pages: 99-106
  • Peer Reviewed
• [Journal Article] Feeding responses to central administration of several somatostatin analogs in chicks. (2011)
  • Author(s): Tachibana T, Cline MA, Khan MSI, Ueda H, Hiramatsu K
  • Journal Title: Comparative Biochemistry and Physiology Part A 158 Pages: 47-51
  • Peer Reviewed
• [Journal Article] Comparison of feeding systems : feed cost, palatability and environmental impact among hay fattened beef, consistent grass-only fed beef and conventional marbled beef in Wagyu (Japanese Black cattle). (2010)
  • Author(s): Sithyphone K., M.Yabe, H.Horita, K.Hayashi, F.Tomiko, Y.Shiotsuka, T.Etoh, F.Ebara, O.Samadmanivong, J.Wegner, T.Gotoh.
  • Journal Title: Animal Science Journal. 82 Pages: 352-359
  • Peer Reviewed
• [Journal Article] Effects of insufficient levels of dietary protein on IGF-1 and IGFBPs in young chickens. (2010)
  • Author(s): Nagao K, Hiramatsu K, Tsukada A, Kita K.
  • Journal Title: The Journa of Poultry Science 47 Pages: 236-239
  • Peer Reviewed
• [Journal Article] Cloning, expression analysis, and regulatory mechanisms of bovine chemerin and chemerin receptor. (2010)
  • Author(s): Song SH, Fukui K.Nakajima K, Kozakai T, Sasaki S, Roh SG (Corresponding Author), Katoh K.
  • Journal Title: Domestic Animal Endocrinology 39(2) Pages: 97-105
  • Peer Reviewed
• [Journal Article] Intensified nursing dramatically accelerates growth performance and the size of the body frame in Japanese Black and Holstein crossbred steers. (2010)
  • Author(s): Inada S., F.Ebara, S.Asaoka, K.sada, Y.Isozaki, A.Saito, T.Gotoh
  • Journal Title: J.Anim. Vet.Adv. 9(6) Pages: 1037-1047
  • Peer Reviewed
• [Journal Article] Intensive nursing and feeding during the early growth period altered intramuscular adipogenesis in crossbred steers (Japanese Black male×Holstein female). (2010)
  • Author(s): Ebara F., S.Inada, S.Asaoka, Y.Isozaki, A.Saito, T.Etoh, Y.Shiotsuka, T.Gotoh
  • Journal Title: J.Anim.Vet.Adv. 9(6) Pages: 982-989
  • Peer Reviewed
• [Journal Article] Cloning, expression analysis, and regulatory mechanisms of bovine chemerin and chemerin receptor (2010)
  • Author(s): Song SH, Fukui K. Nakajima K, Kozakai T, Sasaki S, Rob SG, Katoh K
  • Journal Title: Domestic Animal Endocrinology Volume: 39(2) Pages: 97-105
  • Peer Reviewed
• [Journal Article] Differencesin muscle and fat accretion in Japanese Black and European cattle. (2009)
  • Author(s): Gotoh, T., E.Albrecht, F.Teuscher, K.Kawabata, K.Sakashita, H.Iwamoto, J.Wegner.
  • Journal Title: Meat Science 82 Pages: 300-308
  • Peer Reviewed
• [Journal Article] Prostatic Androgen-Repressed Message-1 as a Regulator of Adipocyte Differentiation in the mouse. (2009)
  • Author(s): Song SH, Hong YH, Sasaki S, Roh SG (Corresponding Author), Katoh K.
  • Journal Title: Tohoku J.of Exp.Med. 219 Pages: 311-317
  • Peer Reviewed
• [Journal Article] Differential action of trans-10, cis-12 conjugated linoleic acid on adipocyte differentiation of ovine and 3T3-L1 preadipocytes. (2009)
  • Author(s): Iga T, Satoh T, Yamamoto S, Fukui K, Song SH, Choi KC, Roh SG, Sasaki S.
  • Journal Title: Asian-Australian Journal of Animal Science. 22(11) Pages: 1566-1573
  • Peer Reviewed
• [Journal Article] Immunohistochemical study on the innervation of the chicken pancreas by pituitary adenylate cyclase-activating polypeptides (PACAPs)-containing nerves. (2009)
  • Author(s): Hiramatsu K, Yamasaki A
  • Journal Title: The Journal of Poultry Science 2009 46 Pages: 234-239
  • Peer Reviewed
• [Journal Article] Central administration of somatostatin stimulates feeding behavior in chicks. (2009)
  • Author(s): Tachibana T, Cline MA, Sugahara K, Ueda H, Hiramatsu K
  • Journal Title: Comparative Biochemistry and Physiology Part A 161 Pages: 354-359
  • Peer Reviewed
• [Journal Article] ニワトリ視床下部における神経ペプチドY(NPY)含有ニューロンの投射経路に関する組織学的研究 (2009)
  • Author(s): 渡辺純、平松浩二
  • Journal Title: 北信越畜産学会報 98 Pages: 23-28
  • NAID: 40016616378
  • Peer Reviewed
• [Journal Article] Depletion of primordial germ cells (PGCs) by X-irradiation to extraembryonic region of chicken embryos and expression of xenotransplanted quail PGCs. (2009)
  • Author(s): Atsumi Y, Yazawa S, Usui F, Nakamura Y, Yamamoto Y, Tagami T, Hiramatsu K, Kagami H, Ono T.
  • Journal Title: The Journal of Poultry Science 46 Pages: 136-143
  • Peer Reviewed
• [Journal Article] Complete regeneration of muscular dystrophy chickens by mating of male and female offspring derived from germline chimeras. (2009)
  • Author(s): Fujiwara A, Ono T, Hiramatsu K, Kagami H.
  • Journal Title: The Journal of Poultry Science 46 Pages: 123-126
  • Peer Reviewed
• [Journal Article] Differential action of trans-10, cis-12 conjugated linoleic acid on adipocyte differentiation of ovine and 313-L1 preadipocytes (2009)
  • Author(s): Iga T, Satoh T, Yamamoto S, Fukui K, Song SH, Choi KC, Roh SG, Sasaki S
  • Journal Title: Asian-Australian Journal of Animal Science Volume: 22(11) Pages: 1566-1573
  • Peer Reviewed
• [Journal Article] Prostatic Androgen-Repressed Message-1 as a Regulator of Adipocyte Differentiation in the mouse. (2009)
  • Author(s): Song SH, Hong YH, Sasaki S, Roh SG (Corresponding Author), Katoh K.
  • Journal Title: Tohoku J.of Exp.Med. Volume: 219 Pages: 311-317
  • Peer Reviewed
• [Journal Article] Central administration of galanin stimulates feeding behavior in chicks. (2008)
  • Author(s): Tachibana T, Mori M, Khan MdSI, Ueda H, Sugahara K, Hiramatsu K.
  • Journal Title: Comparative Biochemistry and Physiology Part A 151 Pages: 637-640
  • Peer Reviewed
• [Presentation] ヒト肝がん細胞(HepG2)におけるChemerin分泌調節 (2010)
  • Author(s): 盧尚建、宋相憲、鈴木裕、Ardiyanti Astrid、加藤和雄
  • Organizer: BMB2010 1P-0029
  • Place of Presentation: 神戸ポートアイランド、神戸
  • Year and Date: 2010-12-08
• [Presentation] ヒト肝がん細胞(HepG2)におけるChemerin分泌調節 (2010)
  • Author(s): 盧尚建、宋相憲、鈴木裕、Ardiyanti Astrid、加藤下和雄
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド、神戸
  • Year and Date: 2010-12-08
• [Presentation] 筋芽細胞および脂肪細胞の脂肪蓄積能におけるAdipogeninの機能解析 (2010)
  • Author(s): 宋相憲、鈴木裕、盧尚建、加藤和雄
  • Organizer: Adiposcience研究会 p.36
  • Place of Presentation: 大阪
  • Year and Date: 2010-08-21
• [Presentation] 脂肪細胞と肝細胞におけるChemerinとChemerin受容体の発現動態 (2010)
  • Author(s): 鈴木裕、宋相憲、盧尚建、加藤和雄
  • Organizer: 講演要旨集Adiposcience研究会 p.33
  • Place of Presentation: 大阪
  • Year and Date: 2010-08-21
• [Presentation] 脂肪細胞と肝細胞におけるChemerinとChemerin受容体の発現動態 (2010)
  • Author(s): 鈴木裕、宋相憲、盧尚建、加藤和雄
  • Organizer: Adiposcience研究会
  • Place of Presentation: 大阪
  • Year and Date: 2010-08-21
• [Presentation] ウシ脂肪細胞におけるケメリン(Chemerin)とその受容体の発現調節機構 (2010)
  • Author(s): 鈴木裕、宋相憲、盧尚建、加藤和雄
  • Organizer: 日本畜産学会第112回大会 P.98
  • Place of Presentation: 東京
  • Year and Date: 2010-03-28
• [Presentation] ウシ乳腺上皮細胞におけるアディポネクチンのシグナル伝達とエネルギー代謝及び増殖、生存関連遺伝子発現に及ぼす影響 (2010)
  • Author(s): 大谷喜永、宋相憲、萩野顕彦、盧尚建、加藤和雄
  • Organizer: 日本畜産学会第112回大会 P.98
  • Place of Presentation: 東京
  • Year and Date: 2010-03-28
• [Presentation] ヒツジとヤギの血中ACTHおよびGH濃度に及ぼす頚静脈内Apelin投与の影響 (2010)
  • Author(s): 佐藤勝祥、高橋辰行、小岩幸太、萩野顕彦、盧尚建、加藤和雄
  • Organizer: 日本畜産学会第112回大会 P.99
  • Place of Presentation: 東京
  • Year and Date: 2010-03-28
• [Presentation] 幼動物へのミルク摂取時に見られる血中GHとインスリン濃度の増加機構 (2010)
  • Author(s): 千田はるか、加藤伸一、佐藤勝祥、高橋辰行、星野由美、盧尚建、加藤和雄
  • Organizer: 日本畜産学会第112回大会 P.100
  • Place of Presentation: 東京
  • Year and Date: 2010-03-28
• [Presentation] 黒毛和種肥育牛の枝肉成績・筋肉内脂肪酸組成とFASNおよびSCD遺伝子多型との関連 (2010)
  • Author(s): 杉田春奈、Astrid Ardiyanti、横田祥子、為岡奈々恵、阿部剛、庄司則章、小林栄治、鈴木啓一、盧尚建、加藤和雄
  • Organizer: 日本畜産学会第112回大会 P.109
  • Place of Presentation: 東京
  • Year and Date: 2010-03-28
• [Presentation] 脂肪細胞由来遺伝子、Adipogeninの発現調節について (2010)
  • Author(s): 盧尚建、宋相憲、鈴木裕、佐々木晋一、加藤和雄
  • Organizer: 日本畜産学会第112回大会 P.110
  • Place of Presentation: 東京
  • Year and Date: 2010-03-28
• [Presentation] Chemerin, highly expressed in adipose tissues, stimulates the glycerol release in bovine differentiated adipocytes in vitro. (2009)
  • Author(s): Song, S.H., Fukui, K., Hamano, K., Sasaki, S., Roh, S.G., Katoh, K.
  • Organizer: Proceeding of XIth International Symposium on Ruminant Physiology
  • Place of Presentation: Clermont-Ferrand, French
  • Year and Date: 2009-09-07
• [Presentation] Overexpression of adipogenin enhances the fat accumulation of C2C12 my oblasts. (2009)
  • Author(s): Song, S.H., Roh, S.G., Katoh K.
  • Organizer: Experimental Biology
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2009-04-22
• [Presentation] Overexpression of adipogenin enhances the fat accumulation of C2C12 myoblasts. (2009)
  • Author(s): Song, S.H., Roh, S.G., Katoh K.
  • Organizer: Experimental Biology 2009
  • Place of Presentation: Anaheim, CA, USA
  • Year and Date: 2009-04-19
• [Presentation] Cloning and expression of bovine chemerin and chemerin receptor. (2008)
  • Author(s): Roh, S.G., Song, S.H., Fukui, K, Satoh, T., Katoh, K., Sasaki, S.
  • Organizer: 6th International Congress on Farm Animal Endocrinology
  • Place of Presentation: The Hotel Roanoke & Conference Center, Roanoke, USA
  • Year and Date: 2008-11-15
• [Presentation] Cloning and expression of bovine chemerin and chemerin receptor (2008)
  • Author(s): Roh SG, Song SH, Fukui K, Satoh T, Katoh K, Sasakis.
  • Organizer: 6^<th> International Congress of Farm Animal Endocrinology
  • Place of Presentation: Virginia, Roanoke
  • Year and Date: 2008-11-14
• [Presentation] Chemerin, highly expressed in adipose tissues, stimulates the glycerol release in bovine differentiated adipocytes in vitro. (2008)
  • Author(s): Song, S.H., Fukui, K., Hamano, K., Sasaki, S., Roh, S.G., Katoh, K.
  • Organizer: Proceeding of XIth International Symposium on Ruminant Physiology p.662-663
  • Place of Presentation: Clermont-Ferrand、France
  • Year and Date: 2008-09-07
• [Presentation] Up-regulation of chemerin and chemerin receptor by TNF-α in bovine differentiated adipocytes in vitro. (2008)
  • Author(s): Roh, S.G., Katoh K.
  • Organizer: Proceeding of XXXVI International Congress of Physiological Science. P4AM-8-4
  • Place of Presentation: 京都
  • Year and Date: 2008-07-29