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Immunological regulation of the female reproductive tract to establish an efficient new artificial insemination system in mammals

Research Project

Project/Area Number 20380154
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionOkayama University

Principal Investigator

FUNAHASHI Hiroaki  Okayama University, 大学院・自然科学研究科, 教授 (50284089)

Co-Investigator(Kenkyū-buntansha) KONDOU Yasuhiro  岡山大学, 大学院・自然科学研究科, 教授 (90108126)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords多核白血球 / 精子 / ウシ / ブタ / カフェイン / 精漿 / 卵黄 / 人工授精 / ラット
Research Abstract

In the female reproductive tracts, immunological system exists to remove sperm from the tract rapidly after mating or artificial insemination. In the present study, we could make clear that both phagocytotic and chemotactic activities of bovine and porcine polymorphonuclear leukocytes (PMNs), which mainly perform in the system, are stimulated in the presence of fresh serum and decreased in the presence of caffeine, heparin, and seminal plasma. Furthermore, PMNs phagocytize equally both active motile and dead sperm.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (22 results)

All 2011 2010 2009 2008

All Journal Article (6 results) (of which Peer Reviewed: 5 results) Presentation (16 results)

  • [Journal Article] Caffeine, dibutyryl cyclic-AMP and heparin affect the chemotactic and phagocytotic activities of neutrophils for boar sperm in vitro.2011

    • Author(s)
      Jing-Chun Li, Shoichiro YAMAGUCHI, Yasuhiro KONDO, Hiroaki FUNAHASHI
    • Journal Title

      Theriogenology. 75

      Pages: 1336-1345

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Caffeine, dibutyryl cyclic-AMP and heparin affect the chemotactic and phagocytotic activities of neutrophils for boar sperm in vitro2011

    • Author(s)
      Jing-Chun Li, Shoichiro YAMAGUCHI, Yasuhiro KONDO, Hiroaki FUNAHASHI
    • Journal Title

      Theriogenology

      Volume: 75 Pages: 1336-1345

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of blood serum, caffeine and heparin on in vitro phagocytosis of frozen-thawed bull sperm by neutrophils derived from the peripheral blood of cows.2010

    • Author(s)
      Jing-Chun LI, Hiroaki FUNAHASHI
    • Journal Title

      Theriogenology 74

      Pages: 691-698

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] グローバルな視点での人工授精の技術開発動向2010

    • Author(s)
      舟橋弘晃
    • Journal Title

      養豚の友 5月号

      Pages: 12-17

    • Related Report
      2010 Final Research Report
  • [Journal Article] Effect of blood serum, caffeine and heparin on in vitro phagocytosis of frozen-thawed bull sperm by neutrophils derived from the peripheral blood of cows2010

    • Author(s)
      Jing-Chun LI, Hiroaki FUNAHASHI
    • Journal Title

      Theriogenology (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improved fertility in gilts and sows after artificial insemination of frozen-thawed boar semen by supplementation of semen extender with caffeine and CaCl_2.2009

    • Author(s)
      Yamaguchi, S., Funahashi, H., Murakami, T.
    • Journal Title

      Journal of Reproduction and Development. 55

      Pages: 645-649

    • NAID

      10026322753

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] The effect of pig seminal plasma and egg yolk on chemotaxis and phagocytosis of neutrophils derived from peripheral blood of pigs and cows.2011

    • Author(s)
      李井春, 舟橋弘晃
    • Organizer
      International Embryo Transfer Society, 37th Annual conference
    • Place of Presentation
      Orlando, FL, USA.
    • Related Report
      2010 Final Research Report
  • [Presentation] The effect of pig seminal plasma and egg yolk on chemotaxis and phagocytosis of neutrophils derived from peripheral blood of pigs and cows2011

    • Author(s)
      Jing-chun Li, Hiroaki Funahashi
    • Organizer
      International Embryo Transfer Society, 37th Annual conference
    • Place of Presentation
      Orlando, FL, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] The influence of caffeine or heparin on chemotaxis and phagocytosis of swine polymorphonuclear leukocytes.2009

    • Author(s)
      李井春, 舟橋弘晃
    • Organizer
      The 4th Congress of Asian Pig Veterinary Society.
    • Place of Presentation
      Tsukuba International Congress Center, Tsukuba
    • Related Report
      2010 Final Research Report
  • [Presentation] Reduction of immunological sperm loss following artificial insemination of boar semen.2009

    • Author(s)
      Hiroaki FUNAHASHI, Jing-Chun LI, Shoichiro YAMAGUCHI, Tetsuya MURAKAMI
    • Organizer
      The 4th Congress of Asian Pig Veterinary Society.
    • Place of Presentation
      Tsukuba International Congress Center, Tsukuba
    • Related Report
      2010 Final Research Report
  • [Presentation] 卵黄含有希釈液の注入がラット子宮内多核白血球数に及ぼす影響2009

    • Author(s)
      藪崎雅紀, 舟橋弘晃
    • Organizer
      第102回日本繁殖生物学会大会
    • Place of Presentation
      近畿大学農学部(奈良市)
    • Related Report
      2010 Final Research Report
  • [Presentation] カフェイン及びヘパリンが豚白血球の走化性と貪食性に及ぼす影響2009

    • Author(s)
      李井春、舟橋弘晃
    • Organizer
      第102回日本繁殖生物学会大会
    • Place of Presentation
      近畿大学農学部(奈良市)
    • Related Report
      2010 Final Research Report
  • [Presentation] 卵黄含有希釈液の注入がラット子宮内多核白血球数に及ぼす影響2009

    • Author(s)
      藪崎雅紀, 舟橋弘晃
    • Organizer
      第102回日本繁殖生物学会大会
    • Place of Presentation
      近畿大学農学部、奈良県奈良市
    • Related Report
      2009 Annual Research Report
  • [Presentation] カフェイン及びヘパリンが豚白血球の走化性と貪食性に及ぼす影響2009

    • Author(s)
      李井春、舟橋弘晃
    • Organizer
      第102回日本繁殖生物学会大会
    • Place of Presentation
      近畿大学農学部、奈良県奈良市
    • Related Report
      2009 Annual Research Report
  • [Presentation] The influence of caffeine or heparin on chemotaxis and phagocytosis of swine polymorphonuclear leukocytes2009

    • Author(s)
      Jing-Chun Li, Funahashi Hiroaki
    • Organizer
      The 4th Congress of Asian Pig Veterinary Society
    • Place of Presentation
      Tsukuba International Congress Center, Tsukuba, Japan
    • Related Report
      2009 Annual Research Report
  • [Presentation] Reduction of immunological sperm loss following artificial insemination of boar semen2009

    • Author(s)
      Hiroaki FUNAHASHI, Jing-Chun LI, Shoichiro YAMAGUCHI,
    • Organizer
      The 4th Congress of Asian Pig Veterinary Society
    • Place of Presentation
      Tsukuba International Congress Center, Tsukuba, Japan
    • Related Report
      2009 Annual Research Report
  • [Presentation] ラット雌性生殖道内に射出された精子の多核白血球による貪食2008

    • Author(s)
      薮崎雅紀、舟橋弘晃
    • Organizer
      ラット生殖工学ワークショップ
    • Place of Presentation
      麻布大学獣医学部(相模原市)
    • Year and Date
      2008-07-20
    • Related Report
      2010 Final Research Report
  • [Presentation] ラット雌性生殖道内に射出された精子の多核白血球による貪食2008

    • Author(s)
      薮崎雅紀, 舟橋弘晃
    • Organizer
      ラット生殖工学ワークショップ
    • Place of Presentation
      麻布大学獣医学部
    • Year and Date
      2008-07-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] 牛多核白血球の精子および血清に対する走化性2008

    • Author(s)
      李井春、薮崎雅紀、舟橋弘晃
    • Organizer
      第101回日本繁殖生物学会大会
    • Place of Presentation
      九州大学医学部(福岡市)
    • Related Report
      2010 Final Research Report
  • [Presentation] ラット子宮内多核白血球の性周期および交配後の変動2008

    • Author(s)
      薮崎雅紀、李井春、舟橋弘晃
    • Organizer
      第101回日本繁殖生物学会大会
    • Place of Presentation
      九州大学医学部(福岡市)
    • Related Report
      2010 Final Research Report
  • [Presentation] 牛多核白血球の精子および血清に対する走化性2008

    • Author(s)
      李井春, 薮崎雅紀, 舟橋弘晃
    • Organizer
      第101回日本繁殖生物学会大会
    • Place of Presentation
      九州大学医学部
    • Related Report
      2008 Annual Research Report
  • [Presentation] ラット子宮内多核白血球の性周期および交配後の変動2008

    • Author(s)
      薮崎雅紀, 李井春, 舟橋弘晃
    • Organizer
      第101回日本繁殖生物学会大会
    • Place of Presentation
      九州大学医学部
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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