Cryopreservation of oocytes and embryos by inducing the expression of cryoprotectant channels.

• Project/Area Number: 20380155
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Kochi University
• Principal Investigator: EDASHIGE Keisuke Kochi University, 教育研究部・総合科学系, 教授 (30175228)
• Co-Investigator(Kenkyū-buntansha): KASAI Magosaburo 高知大学, 教育研究部・総合科学系, 教授 (60152617) ; KOSHIMOTO Chihiro 宮崎大学, フロンティア科学実験総合センター, 教授 (70295210)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2010: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2009: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2008: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
• Keywords: 凍結保存 / 卵子 / 胚 / 水透過性 / 耐凍剤透過性 / アクアポリン / 受精卵 / 促進拡散

Research Abstract

We tried to develop a vitrification method by which mouse oocytes and embryos at various stages can be cryopreserved by a single method. First, we examined whether the membrane-permeability of mouse oocytes and embryos can be equalized, because the permeability largely affects the condition for vitrification. We tried to induce the expression of water/cryoprotectant channels (AQP) in oocytes to increase the low membrane-permeability. We also tried in morulae to decrease the permeability of AQP3 which is markedly expressed in morulae and blastocysts. The cryoprotectant-permeability of oocytes increased slightly by retinoic acid but the maturation rate decreased remarkably. It was reported that the permeability of AQP3 decreased markedly at low pH. However, the permeability to ethylene glycol of mouse morulae, which expressed AQP3 abundantly, increased at low pH. Therefore, we considered that it would be difficult to make the membrane-permeability of oocytes/embryos equal. Next, we tried to develop a new vitrification solution in which a high concentration of sucrose was included to reduce the effect of membrane-permeability of oocytes/embryos on the vitrification condition. With this solution, embryos from the 2-cell to morula stages were successfully vitrified in the same condition, and vitrified embryos could be transported with dry ice (at -79℃). The method would be useful for cryopreservation of mouse embryos at various stages.

Research Products

• [Journal Article] Developmental ability of vitrified mouse oocytes expressing water channels. (2011)
  • Author(s): Yohei Yamaji, Shinsuke Seki, Kazutsugu Matsukawa, Chihiro Koshimoto, Magosaburo Kasai, Keisuke Edashige.
  • Journal Title: The Journal of Reproduction and Development 57 Pages: 403-108
  • NAID: 10029050757
  • Peer Reviewed
• [Journal Article] Cryobiological properties of immature zebrafish oocytes assessed by their ability to be fertilized and develop into hatching embryos. (2011)
  • Author(s): Shinsuke Seki, Toshimitsu Kouya, Ryoma Tsuchiya, Delgado M.Valdez Jr., Bo Jin, Chihiro Koshimoto, Magosaburo Kasai, Keisuke Edashige.
  • Journal Title: Cryobiology 62 Pages: 8-14
  • Peer Reviewed
• [Journal Article] Human C-reactive protein enhances thrombus formation after neointimal balloon injury in transgenic rabbits. (2011)
  • Author(s): S.Matsuda, A.Yamashita, Y.Sato, S.Kitajima, T.Koike, C.Sugita, S.Moriguchi-Goto, K.Hatakeyama, M.Takahashi, C.Koshimoto, Y. Matsuura, T.Iwakiri, Y.E. Chen, J.Fan, Y.Asada.
  • Journal Title: The Journal of Thrombosis and Haemostasis 9 Pages: 201-208
  • Peer Reviewed
• [Journal Article] Human C-reactive protein enhances thrombus formation after neointimal balloon injury in transgenic rabbits (2011)
  • Author(s): Matsuda S, Yamashita A, Sato Y, Kitajima S, Koike T, Sugita C, Moriguchi-Goto S, Hatakeyama K, Takahashi M, Koshimoto C, Matsuura Y, Iwakiri T, Chen YE, Fan J, Asada Y.
  • Journal Title: The Journal of Thrombosis and Haemostasis Volume: 9 Pages: 201-208
  • Peer Reviewed
• [Journal Article] Cryobiological properties of immature zebrafish oocytes assessed by their ability to be fertilized and develop into hatching embryos (2011)
  • Author(s): Seki S, Kouya T, Tsuchiya R, Valdez DM Jr, Jin B, Koshimoto C, Kasai M, Edashige K.
  • Journal Title: Cryobiology Volume: 62 Pages: 8-14
  • Peer Reviewed
• [Journal Article] Developmental ability of vitrified mouse oocytes expressing water channels (2011)
  • Author(s): Yamaji Y, Seki S, Matsukawa K, Koshimoto C, Kasai M, Edashige K.
  • Journal Title: The Journal of Reproduction and Development Volume: (in press)
  • NAID: 10029050757
  • Peer Reviewed
• [Journal Article] Equilibrium vitrification of mouse embryos. (2010)
  • Author(s): Bo Jin, Keiji Mochida, Atsuo Ogura, Eri Hotta, Yukiko Kobayashi, Kaori Ito, Go Egawa, Shinsuke Seki, Hiroshi Honda, Keisuke Edashige, Magosaburo Kasai.
  • Journal Title: Biology of Reproduction 82 Pages: 444-450
  • Peer Reviewed
• [Journal Article] Effects of bile acids on rat hepatic microsomal type I 11β-hydroxyl steroid dehydrogenase. (2010)
  • Author(s): Yorio Maeda, Seiji Naganuma, Ichiro Niina, Akio Shinohara, Chihiro Koshimoto, Kazuhiro Kondo, Kazuo Chijiiwa.
  • Journal Title: Steroids 75 Pages: 164-168
  • Peer Reviewed
• [Journal Article] Equilibrium vitrification of mouse embryos (2010)
  • Author(s): Jin B, Mochida K, Ogura A, Hotta E, Kobayashi Y, Ito K, Egawa G, Seki S, Honda H, Edashige K, Kasai M.
  • Journal Title: Biology of Reproduction Volume: 82 Pages: 444-450
  • Peer Reviewed
• [Journal Article] Effects of bile acids on rat hepatic microsomal type I 11beta-hydroxysteroid dehydrogenase (2010)
  • Author(s): Maeda Y, Naganuma S, Niina I, Shinohara A, Koshimoto C, Kondo K, Chijiiwa K.
  • Journal Title: Steroids Volume: 75 Pages: 164-168
  • Peer Reviewed
• [Journal Article] Effects of bile acids on rat hepatic microsomal type I 11 beta-hydroxysteroid dehydrogenase (2010)
  • Author(s): Yorio Maeda
  • Journal Title: Steroids 75 Pages: 164-168
  • Peer Reviewed
• [Journal Article] Equilibrium vitrification of mouse embryos (2010)
  • Author(s): Bo Jin
  • Journal Title: Biology of Reproduction 82 Pages: 444-450
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): Magosaburo Kasai
  • Journal Title: Movement of water and cryoprotectants in mouse oocytes and embryos at different stages : relevance to cryopreservation. In : Fertility Cryopreservation(Chian R, Quinn P(eds.))(Cambridge University Press, Cambridge, U.K.) Pages: 16-23
• [Journal Article] Critical role of von Willebr and factorand platelet interaction in venous thromboembolism. (2009)
  • Author(s): Misaki Takahashi, Atsushi Yamashita, Sayaka Moriguchi-Goto, Kousuke Marutsuka, Yuichiro Sato, Hiroshi Yamamoto, Chihiro Koshimoto, Yujiro Asada.
  • Journal Title: Histology and Histopathology 24 Pages: 1391-1398
  • Peer Reviewed
• [Journal Article] Maintenance of fertility in cryopreserved Indian gerbil (Tatera indica) spermatozoa. (2009)
  • Author(s): Chihiro Koshimoto, Daisuke Watanabe, Akio Shinohara, Tetsuo Morita.
  • Journal Title: Cryobiology 58 Pages: 303-307
  • Peer Reviewed
• [Journal Article] Analysis of antibody response by temperature-sensitive measles vaccine strain in the cotton rat model. (2009)
  • Author(s): Takeshi Haga, Niho Murayama, Yuya Shimizu, Akatsuki Saito, Takumi Sakamoto, Tetsuo Morita, Katsuhiro Komase, Tetsuo Nakayama, Kazuyuki Uchida, Tetsuro Katayama, Akio Shinohara, Chihiro Koshimoto, Hiroshi Sato, Hironori Miyata, Kiyoaki Katahira, Yoshitaka Goto.
  • Journal Title: Comparative Immunology, Microbiology and Infectious Diseases 32 Pages: 395-406
  • Peer Reviewed
• [Journal Article] Analysis of antibody response by temperature-sensitive measles vaccine strain in the cotton rat model (2009)
  • Author(s): Takeshi Haga
  • Journal Title: Comparative Immunology, Microbiology and Infectious Diseases 32 Pages: 395-406
  • Peer Reviewed
• [Journal Article] Maintenance of fertility in cryopreserved Indian gerbil(Tatera indica)spermatozoa (2009)
  • Author(s): Chihiro Koshimoto
  • Journal Title: Cryobiology 58 Pages: 303-307
  • Peer Reviewed
• [Journal Article] Critical role of von Willebrand factor and platelet interaction in venous thromboembolism (2009)
  • Author(s): Misaki Takahashi
  • Journal Title: Histology and Histopathology 24 Pages: 1391-1398
  • Peer Reviewed
• [Journal Article] The mechanism by which mouse spermatozoa are injured during freezing. (2008)
  • Author(s): Bo Jin, Chihiro Yamasaki, Naoko Yamada, Shinsuke Seki, Delgado M. Valdez Jr., Magosaburo Kasai, Keisuke Edashige.
  • Journal Title: Journal of Reproduction and Development 54 Pages: 265-269
  • NAID: 10024289393
  • Peer Reviewed
• [Journal Article] Formation of extracellular and intracellular ice during warming of vitrified mouse morulae and its effect on embryo survival. (2008)
  • Author(s): Bo Jin, Kenji Kusanagi, Makiko Ueda, Shinsuke Seki, Delgado M. Valdez Jr., Keisuke Edashige, Magosaburo Kasai.
  • Journal Title: Cryobiology 56 Pages: 233-240
  • Peer Reviewed
• [Journal Article] Development of a reliable in vitro maturation system for zebrafish oocytes. (2008)
  • Author(s): Shinsuke Seki, Toshimitsu Kouya, Ryoma Tsuchiya, Delgado M.Valdez Jr., Bo Jin, Takao Hara, Naoya Saida, Magosaburo Kasai, Keisuke Edashige.
  • Journal Title: Reproduction 135 Pages: 285-292
  • Peer Reviewed
• [Journal Article] Development of a reliable in vitro maturation system forzebrafi sh oocytes. (2008)
  • Author(s): Shinsuke Seki, Toshimitsu Kouya, Ryoraa Tsuchiya, Delgado M. Valdez Jr., Bo Jin, Takao Hara, Naoya Saida, Magosaburo Kasai, Keisuke Edashige
  • Journal Title: Reproduction 135 Pages: 285-292
  • Peer Reviewed
• [Journal Article] Formation of extracellular and intracellular ice during warming of vitrified mouse morulae and its effect on embryo survival. (2008)
  • Author(s): Bo Tin, Kenji Kusanagi, Makiko Ueda, Shinsuke Seki, Delgado M. Valdez Jr., Keisuke Edashige, Magosaburo Kasai
  • Journal Title: Cryobiology 56 Pages: 233-240
  • Peer Reviewed
• [Journal Article] The mechanism by which mouse spermatozoa are injured during freezing. (2008)
  • Author(s): Bo Jin, Chihiro Yamasaki, Naoko Yamada, Shinsuke Seki, Delgado M. Valdez Jr., Magosaburo Kasai, Keisuke Edashiee
  • Journal Title: Journal of Reproduction and Development 54 Pages: 265-269
  • NAID: 10024289393
  • Peer Reviewed
• [Presentation] マウスの卵子および胚への耐凍剤透過におけるAquaporin 9の役割 (2010)
  • Author(s): 有村隼, 中田裕一, 関信輔, 松尾完, 金波, 松川和嗣, 葛西孫三郎, 枝重圭祐
  • Organizer: 第103回日本繁殖生物学会大会
  • Place of Presentation: 十和田市,北里大学獣医学部
• [Presentation] 凍結乾燥したウシ体細胞による核移植胚作出の試み (2010)
  • Author(s): 長尾さや子, 長瀬祐樹, 枝重圭祐, 葛西孫三郎, 保地眞一, 松川和嗣
  • Organizer: 第103回日本繁殖生物学会大会
  • Place of Presentation: 十和田市,北里大学獣医学部
• [Presentation] Aquaporin 9 facilitates the movement of DMSO and acetamide through the plasma membrane in mouse morulae. (2010)
  • Author(s): Keisuke Edashige, Shinsuke Seki, Hayato Arimura, Kan Matsuo, Yu-ichi Nakata, Bo Jin, Kazutsugu Matsukawa, Chihiro Koshimoto, Magosaburo Kasai
  • Organizer: 第15回中国畜産獣医学会(動物繁殖分会)
  • Place of Presentation: Tianjin, Ruby Hotel.
• [Presentation] The role of aquaporin 9 in the movement of Me_2SO and acetamide in mouse morulae. (2010)
  • Author(s): Keisuke Edashige, Shinsuke Seki, Hayato Arimura, Kan Matsuo, Yu-ichi Nakata, Bo Jin, Chihiro Koshimoto, Magosaburo Kasai
  • Organizer: 47th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Bristol, Bristol Royal Marriott Hotel.
• [Presentation] Handy transportation of vitrified mouse embryos with dry ice. (2010)
  • Author(s): Bo Jin, Keiji Mochida, Atsuo Ogura, Magosaburo Kasai, Keisuke Edashige
  • Organizer: 47th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Bristol, Bristol Royal Marriott Hotel.
• [Presentation] 耐凍剤チャンネル発現の誘導によるマウス卵子の耐凍性向上の試み (2010)
  • Author(s): 和田紗希子, 中田裕一, 金波, 越本知大, 葛西孫三郎, 枝重圭祐
  • Organizer: 第57回日本実験動物学会総会
  • Place of Presentation: 京都,京都テルサ
• [Presentation] LASセミナー3:胚・精子の凍結保存 (2010)
  • Author(s): 中潟直己, 枝重圭祐
  • Organizer: 第57回日本実験動物学会総会
  • Place of Presentation: 京都,京都テルサ
• [Presentation] Handy transportation of vitrified mouse embryos with dry ice (2010)
  • Author(s): Jin B, Mochida K, Ogura A, Kasai M, Edashige, K.
  • Organizer: 47th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Bristol, Bristol Royal Marriott Hotel(イギリス)
• [Presentation] The role of aquaporin 9 in the movement of Me_2SO and acetamide in mouse morulae (2010)
  • Author(s): Edashige K, Seki S, Arimura H, Matsuo K, Nakata Y, Jin B, Koshimoto C, Kasai M.
  • Organizer: 47th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Bristol, Bristol Royal Marriott Hotel(イギリス)
• [Presentation] Aquaporin 9 facilitates the movement of DMSO and acetamide through the plasma membrane in mouse morulae. (2010)
  • Author(s): Edashige K, Seki S, Arimura H, Matsuo K, Nakata Y, Jin B, Matsukawa K, Koshimoto C, Kasai M.
  • Organizer: 第15回中国畜産獣医学会(動物繁殖分会)
  • Place of Presentation: 天津, Ruby Hotel(中国)
• [Presentation] 凍結乾燥したウシ体細胞による核移植胚作出の試み (2010)
  • Author(s): 長尾さや子, 長瀬祐樹, 枝重圭祐, 葛西孫三郎, 保地眞一, 松川和嗣
  • Organizer: 第103回日本繁殖生物学会
  • Place of Presentation: 十和田市,北里大学獣医学部(青森県)
• [Presentation] マウスの卵子および胚への耐凍剤透過におけるAquaporin 9の役割 (2010)
  • Author(s): 有村隼, 中田裕一, 関信輔, 松尾完, 金波, 松川和嗣, 葛西孫三郎, 枝重圭祐
  • Organizer: 第103回日本繁殖生物学会
  • Place of Presentation: 十和田市,北里大学獣医学部(青森県)
• [Presentation] マウス卵巣のガラス化凍結保存 (2009)
  • Author(s): 金波, 本田宙, 西門優, 葛西孫三郎, 枝重圭祐
  • Organizer: 第102回日本繁殖生物学会大会
  • Place of Presentation: 奈良,近畿大学農学部
• [Presentation] Effect of the expression of aquaporins 1 and 3 in mouse MII oocytes on the nucleation temperature for intracellular ice formation. (2009)
  • Author(s): Shinsuke Seki, Keisuke Edashige, Peter Mazur
  • Organizer: 46th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Sapporo, Conference Hall on Hokkaido University.
• [Presentation] Rapid movement of water and cryoprotectants in pig expanded blastocysts via channel processes -its relevance to their tolerance to cryopreservation-. (2009)
  • Author(s): Keisuke Edashige, Bo Jin, Ryu-ichi Higashiyama, Jun-ichi Yonezawa, Masashi Miyake, Sei-ichi Takahashi, Ken-ichi Yazawa, Magosaburo Kasai
  • Organizer: 46th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Sapporo, Conference Hall on Hokkaido University.
• [Presentation] Equilibrium vitrification of mouse embryos. (2009)
  • Author(s): Bo Jin, Eri Hotta, Yukiko Kobayashi, Kaori Ito, Go Egawa, Shinsuke Seki, Hiroshi Honda, Keiji Mochida, Atsuo Ogura, Keisuke Edashige, Magosaburo Kasai
  • Organizer: 46th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Sapporo, Conference Hall on Hokkaido University.
• [Presentation] Survival of oocytes in antral follicles of vitrified mouse ovaries. (2009)
  • Author(s): Keisuke Edashige, Delgado M. Valdez Jr., Hiroshi Honda, Yu Nishikado, Bo Jin, Magosaburo Kasai
  • Organizer: 46th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Sapporo, Conference Hall on Hokkaido University.
• [Presentation] ドライシッパーを用いないマウス胚の冷蔵及びドライアイス温度下での輸送の検討 (2009)
  • Author(s): 持田慶司, 鬼頭靖司, 金波, 枝重圭祐, 葛西孫三郎, 小倉淳郎
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 大宮,大宮ソニックシティ
• [Presentation] 様々な発育ステージのガラス化凍結マウス胚の-80℃での短期保持-ドライアイスを用いた簡便な輸送を目指して- (2009)
  • Author(s): 金波, 堀田英里, 小林由希子, 伊藤香, 関信輔, 本田宙, 持田慶司, 小倉淳郎, 葛西孫三郎, 枝重圭祐
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 大宮,大宮ソニックシティ
• [Presentation] LASセミナー3:胚・精子の凍結保存 (2009)
  • Author(s): 中潟直己, 枝重圭祐
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 大宮,大宮ソニックシティ
• [Presentation] ブタの卵子と拡大胚盤胞における水と耐凍剤の透過経路 (2009)
  • Author(s): 金波, 東山龍一, 米澤潤一, 高橋誠一, 三宅正史, 葛西孫三郎, 枝重圭祐
  • Organizer: 第110回日本畜産学会大会
  • Place of Presentation: 藤沢市,日本大学生物資源科学部
• [Presentation] 胚・精子の凍結保存 (2009)
  • Author(s): 中潟直己
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 大宮ソニックシティ(埼玉県)
• [Presentation] 様々な発育ステージのガラス化凍結マウス胚の-80℃での短期保持-ドライアイスを用いた簡便な輸送を目指して- (2009)
  • Author(s): 金波
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 大宮ソニックシティ(埼玉県)
• [Presentation] ドライシッパーを用いないマウス胚の冷蔵及びドライアイス温度下での輸送の検討 (2009)
  • Author(s): 持田慶司
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 大宮ソニックシティ(埼玉県)
• [Presentation] Equilibrium vitrification of mouse embryos (2009)
  • Author(s): 金波
  • Organizer: 46th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: The Conference Hall, Hokkaido University(Hokkaido)
• [Presentation] Survival of oocytes in antral follicles of vitrified mouse ovaries (2009)
  • Author(s): Keisuke Edashige
  • Organizer: 46th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: The Conference Hall, Hokkaido University(Hokkaido)
• [Presentation] Rapid movement of water and cryoprotectants in pig expanded blastocysts via channel processes -its relevance to their tolerance to cryopreservation- (2009)
  • Author(s): Keisuke Edashige
  • Organizer: 46th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: The Conference Hall, Hokkaido University(Hokkaido)
• [Presentation] Effect of the expression of aquaporins 1 and 3 in mouse MII oocytes on the nucleation temperature for intracellular ice formation (2009)
  • Author(s): Shinsuke Seki
  • Organizer: 46th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: The Conference Hall, Hokkaido University(Hokkaido)
• [Presentation] マウス卵巣のガラス化凍結保存 (2009)
  • Author(s): 金波
  • Organizer: 第102回日本繁殖生物学会大会
  • Place of Presentation: 近畿大学農学部(奈良県)
• [Presentation] ブクの卵子と拡大胚盤胞における水と耐凍剤の透過経路 (2009)
  • Author(s): 金 波, 東山 龍一, 米澤 潤一, 高橋 誠一, 三宅 正史, 葛西 孫三郎, 枝重 圭祐
  • Organizer: 第110回日本畜産学会大会
  • Place of Presentation: 神奈川県藤沢市日本大学生物資源学部
• [Presentation] Exogenous expression of rat aquaporin-3 enhances permeability to water and cryoprotectants of immature oocytes in the zebrafish (Danio rerio). (2008)
  • Author(s): Shinsuke Seki, Bo Jin, Tsuchiya Ryouma, Toshimitsu Kouya, Takao Hara, Delgado M.Valdez Jr., Magosaburo Kasai, Keisuke Edashige.
  • Organizer: The 5th Annual Conference of the Asia Reproductive Biotechnology Society
  • Place of Presentation: Kunming, Horizon Hotel.
• [Presentation] Short-term storage of vitrified two-cell mouse embryos at -80oC for handy transportation with dry ice. (2008)
  • Author(s): Bo Jin, Eri Hotta, Yukiko Kobayashi, Shinsuke Seki, Masahiro Yoshimura, Hiroshi Honda, Keiji Mochida, Atsuo Ogura, Magosaburo Kasai, Keisuke Edashige
  • Organizer: 第101回日本繁殖生物学会大会
  • Place of Presentation: 福岡市,九州大学医学部百年講堂
• [Presentation] マウス桑実胚の耐凍剤透過性におけるaquaporin 9の役割 (2008)
  • Author(s): 松尾完, 関信輔, 金波, Valdez M.Delgado Jr., 太田悟史, 好村正博, 葛西孫三郎, 枝重圭祐
  • Organizer: 第101回日本繁殖生物学会大会
  • Place of Presentation: 福岡市,九州大学医学部百年講堂
• [Presentation] マウス精子が凍結保存によって受ける傷害のメカニズム (2008)
  • Author(s): 好村正博, 金波, 山崎千寛, 山田奈央子, 関信輔, Valdez M.Delgado Jr., 葛西孫三郎, 枝重圭祐
  • Organizer: 第101回日本繁殖生物学会大会
  • Place of Presentation: 福岡市,九州大学医学部百年講堂
• [Presentation] ウシ桑実胚の水透過と耐凍剤透過におけるaquaporin 3の役割 (2008)
  • Author(s): 河合泰典, 原隆夫, 松尾完, Valdez M.Delgado Jr., 金波, 好村正博, 東山龍一, 市川恭子, 恒石望太郎, 枝重圭祐, 葛西孫三郎
  • Organizer: 第101回日本繁殖生物学会大会
  • Place of Presentation: 福岡市,九州大学医学部百年講堂
• [Presentation] Effects of raffinose concentration and methyl-beta cyclodextrin on IVF with cryopreserved C57BL/6 sperm. (2008)
  • Author(s): Keiji Mochida, Chihiro Koshimoto, Keisuke Edashige, Kentaro Aizawa, Kyuichi Taguma, Akihiko Ohta, Atsuo Ogura
  • Organizer: 45th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Charlotte, The Omni Charlotte Hotel.
• [Presentation] The pathway for the movement of water and cryoprotectants in bovine oocytes and embryos. (2008)
  • Author(s): Keisuke Edashige, Takao Hara, Yasunori Kawai, Masahiro Yoshimura, Bo Jin, Delgado M.Valdez, Jr., Magosaburo Kasai
  • Organizer: 45th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Charlotte, The Omni Charlotte Hotel.
• [Presentation] Permeability to water and cryoprotectants of zebrafish (Danio Rerio) oocytes at mid stage III. (2008)
  • Author(s): Delgado M.Valdez Jr., Ryoma Tsuchiya, Shinsuke Seki, Naoya Saida, Bo Jin, Masahiro Yoshimura, Chihiro Koshimoto, Magosaburo Kasai, Keisuke Edashige
  • Organizer: 45th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Charlotte, The Omni Charlotte Hotel.
• [Presentation] 動物細胞と凍結-哺乳動物の卵子と受精卵の耐凍性に関わるタンパク質- (2008)
  • Author(s): 枝重圭祐
  • Organizer: 第54回日本生物工学会大会セミナー
  • Place of Presentation: 石川郡野々市町,石川県立大学
• [Presentation] 動物細胞と凍結-哺乳動物の卵子と受精卵の耐凍性に関わるタンパク質- (2008)
  • Author(s): 枝重圭祐
  • Organizer: 第54回日本生物工学会大会
  • Place of Presentation: 石川県石川郡野々市町石川県立大学
• [Presentation] Permeability to water and cryoprotectants of zebrafish (Danio Rerio) oocytes at mid stage III. (2008)
  • Author(s): Delgado M. Valdez, Jr., Ryoma Tsuchiya, Shinsuke Seki, Naoya Saida, Bo Jin, Masahiro Yoshimura, Chihiro Koshimoto, Magosaburo Kasai, Keisuke Edashige
  • Organizer: The 45th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: The Omni Charlotte Hotel, Charlotte, NC, USA
• [Presentation] The pathway for the movement of water and cryoprotectantsin bovine oocytes and embryos. (2008)
  • Author(s): Keisuke Edashige, Takao Hara, Yasunori Kawai, Masahiro Yoshimura, Bo Jin, Delgado M. Valdez, Jr., Magosaburo Kasai
  • Organizer: The 45th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: The Omni Charlotte Hotel, Charlotte, NC, USA
• [Presentation] Effects of raffinose concentration and methyl-beta-cyclodextrin on IVF with cryopreserved C57BL/6 sperm. (2008)
  • Author(s): Keiji Mochida, Chihiro Koshimoto, Keisuke Edashige, Kentaro Aizawa, Kyuichi Taguma, Akihiko Ohta, Atsuo Ogura
  • Organizer: The 45th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: The Omni Charlotte Hotel, Charlotte, NC, USA
• [Presentation] ウシ桑実胚の水透過と耐凍剤透過におけるaquaporin 3 の役割 (2008)
  • Author(s): 河合 泰典, 原 隆夫, 松尾 完, Valdez M. Delgado Jr., 金 波, 好村 正博, 東山 龍一, 市川 恭子, 恒石 望太郎, 枝重 圭祐, 葛西 孫三郎
  • Organizer: 第101回日本繁殖生物学会
  • Place of Presentation: 福岡県福岡市九州大学医学部
• [Presentation] マウス桑実胚の耐凍剤透過性におけるaquaporin 9の役割 (2008)
  • Author(s): 松尾 完, 関 信輔, 金 波, Valdez M. Delgado Jr., 太田 悟史, 好村 正博, 葛西 孫三郎, 枝重 圭祐
  • Organizer: 第101回日本繁殖生物学会
  • Place of Presentation: 福岡県福岡市九州大学医学部
• [Presentation] マウス精子が凍結保存によって受ける傷害のメカニズム. (2008)
  • Author(s): 好村 正博, 金 波, 山崎 千寛, 山田 奈央子, 関 信輔, Valdez M. Delgado Jr., 葛西 孫三郎, 枝重圭祐
  • Organizer: 第101回日本繁殖生物学会
  • Place of Presentation: 福岡県福岡市九州大学医学部
• [Presentation] Short-term storage of vitrified two一cell mouse embryos at-80°C for handy transportation with dry ice. (2008)
  • Author(s): Bo Jin, Eri Hotta, Yukiko Kobayashi, Shinsuke Seki, Masahiro Yoshimura, Hiroshi Honda, Keiji, Mochida, Atsuo Ogura, Magosaburo Kasai, Keisuke Edashige
  • Organizer: 第101回日本繁殖生物学会
  • Place of Presentation: 福岡県福岡市九州大学医学部
• [Presentation] Exogenous expression of rat aquaporin-3 enhances permeability to water and cryoprotectants of immature oocytes in the zebrafish (Danio rerio). (2008)
  • Author(s): Shinsuke Seki, Bo Jin, Tsuchiya Ryouma, Toshimitsu Kouya, Takao Hara, Delgado M. Valdez Jr., Magosaburo Kasai, Keisuke Edashige
  • Organizer: The 5th Annual Conference of the Asia Reproductive Biotechnology Society
  • Place of Presentation: Horizon Hotel, Kunming, Yunnan Province, China
• [Book] 生殖系列細胞の保存.生命の誕生に向けて(第二版)生殖補助医療(ART)培養の理論と実際(日本哺乳動物卵子学会編) (2011)
  • Author(s): 葛西孫三郎, 枝重圭祐
  • Publisher: 近代出版
• [Book] Fertility Cryopreservation(Movement of water and cryoprotectants in mouse oocytes and embryos at different stages : relevance to cryopreservation)(Ri-Cheng Chian, Patrick Quinn (Eds.)) (2010)
  • Author(s): 葛西孫三郎, 枝重圭祐
  • Publisher: Cambridge University Press
• [Book] 生殖系列細胞の保存(生命の誕生に向けて(第二版)生殖補助医療(ART)培養の理論と実際(日本哺乳動物卵子学会編) (2010)
  • Author(s): 葛西孫三郎, 枝重圭祐
  • Publisher: 近代出版
• [Book] Movement of water and cryoprotectants in mouse oocytes and embryos at different stages : relevance to cryopreservation, In "Fertility Cryopreservation"(Ri-Cheng Chian & Patrick Quinn (Eds.)) (2010)
  • Author(s): Kasai M, Edashige K.
  • Publisher: Cambridge University Press, Cambridge.