Studies on the molecular mechanisms for Marek's disease virus to increase its virulence in the field

• Project/Area Number: 20380157
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Hokkaido University
• Principal Investigator: OHASHI Kazuhiko Hokkaido University, 大学院・獣医学研究科, 教授 (90250498)
• Co-Investigator(Kenkyū-buntansha): OCHIAI Kenji 北海道大学, 大学院・獣医学研究科, 准教授 (80214162) ; KONNAI Satoru 北海道大学, 大学院・獣医学研究科, 准教授 (40396304)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2009: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2008: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
• Keywords: 病原微生物 / マレック病 / マレック病ウイルス

Research Abstract

To clarify the molecular mechanisms for Marek's disease virus (MDV) to increase its virulence, several viral genes of MDV strains recently isolated from the field were analyzed. Diversities/polymorphisms were identified in one of the MDV genes, meq, that could be potentially related to the increase in MDV virulence. When we analyzed the effects of these diversities/polymorphisms in the meq gene on the functions of the meq gene product (Meq), the amino acid substitutions resulted from the diversities/polymorphisms significantly altered the transactivation and transforming activities of Meq. These findings suggest that the diversities/polymorphisms in the meq gene could contribute to the recent increase in MDV virulence in the field.

Research Products

• [Journal Article] Analysis of transcriptional activities of the Meq proteins present in highly virulent Marek's disease virus strains, RB1B and Md5 (2011)
  • Author(s): Murata S., Konnai S., Ohashi K.
  • Journal Title: Virus genes (印刷中)
  • Peer Reviewed
• [Journal Article] Molecular cloning and expression analysis of bovine programmed death-1 (2010)
  • Author(s): Ikebuchi R., Konnai S., Sunden Y., Onuma,M., Ohashi K.
  • Journal Title: Microbiol.Immunol. 54 Pages: 291-298
  • NAID: 10029544893
  • Peer Reviewed
• [Journal Article] The detection and characterization of the meq variants of Marek's disease virus (2010)
  • Author(s): Ohashi, K., et al
  • Journal Title: Anima Viruses (Maeda, A.ed.) Pages: 117-132
• [Journal Article] Cytokine profiles in chickens infected with virulent and avirulent Marek's disease viruses : interferon-gamma is a key factor in the protection of Marek's disease by vaccination (2009)
  • Author(s): Kano R., Konnai S., Onuma M., Ohashi K.
  • Journal Title: Microbiol.Immunol 53 Pages: 224-232
  • NAID: 10027686336
  • Peer Reviewed
• [Journal Article] Microarray analysis of host immune responses to Marek's disease virus infection in vaccinated chickens (2009)
  • Author(s): Kano R., Konnai S., Onuma M., Ohashi K.
  • Journal Title: J.Vet.Med.Sci. 71 Pages: 603-610
  • NAID: 130000134371
  • Peer Reviewed
• [Journal Article] Comparative moleculo-inmunological analysis of swamp and riverine type water buffaloes responses (2009)
  • Author(s): Mingala C.N., Konnai S., Cruz L.C., Onuma M., Ohashi K.
  • Journal Title: Cytokine 46 Pages: 273-282
  • Peer Reviewed
• [Journal Article] Effect of vaccination with a recombinant metalloprotease from Haemaphysalis longicornis (2009)
  • Author(s): Imamura S., da Silva Vaz I.Jr., Konnai S., Yamada S., Najajima C., Onuma M., Ohashi K.
  • Journal Title: Exp.Appl.Acarol. 48 Pages: 345-358
  • Peer Reviewed
• [Journal Article] Identification and characterization of antimicrobial peptides, defensin, in the taiga tick Ixodes persulcatus (2009)
  • Author(s): Saito Y., Konnai S., Yamada S., Imamura S., Nishikado H., Ito T., Onuma M., Ohashi K.
  • Journal Title: Insect Mol.Biol. 18 Pages: 531-539
  • Peer Reviewed
• [Journal Article] Quantification of water buffalo (Bubal us bubalis) cytokine expression in response to inactivated foot-and-mouth disease (FMD) vaccine (2009)
  • Author(s): Mingala C.N., Konnai S., Venturina A., Onuma M., Ohashi K.
  • Journal Title: Res.Vet.Sci. 87 Pages: 213-217
  • Peer Reviewed
• [Journal Article] Classification of new BVDV isolates from Philippine water buffalo using the viral E2 region (2009)
  • Author(s): Mingala C.N., Konnai S., Onuma M., Ohashi K.
  • Journal Title: J.Basic Microbiol. 49 Pages: 495-500
  • Peer Reviewed
• [Journal Article] Molecular detection of trypanosomes in cattle in South America and genetic diversity of Trypanosoma evansi based on expression-site-associated gene 6 (2009)
  • Author(s): Mekata H., Konnai S., Witola W.H., Inoue N., Onuma M., Ohashi K.
  • Journal Title: Infect.Genet.Evol. 9 Pages: 1301-1305
  • Peer Reviewed
• [Journal Article] Comparative immunogenicity of Haemaphysa Us longicornis and Riphicephalus (Boophilus) microplus calreticulins (2009)
  • Author(s): Parizi L.F., Rech H., Ferreira C.A., Imamura S., Ohashi K., Onuma M., Masuda A., da Silva Vaz I.Jr.
  • Journal Title: Vet.Parasitol. 164 Pages: 282-290
  • Peer Reviewed
• [Journal Article] Cytokine profiles in chickens infected with virulent and avirulent Marek's disease viruses : interferon-gamma is a key factor in the protection of Marek's disease by vaccination (2009)
  • Author(s): Kano, R., et al.
  • Journal Title: Microbiology and Immunology 53 Pages: 224-232
  • Peer Reviewed
• [Journal Article] Cytokine profiles in chickens infected with virulent and avirulent Marek's disease viruses: interferon-gamma is a key factor in the protection of Marek's disease by vaccination (2009)
  • Author(s): Kano, R., et.al.
  • Journal Title: Microbiology and Immunology (印刷中)
• [Journal Article] Cytokine responses in camels (Camelus bactrianus) vaccinated with Brucella abortus strain 19 vaccine (2008)
  • Author(s): Odbileg R., Purevtseren B., Gantsetseg D., Boldbaatar B., Buyannemekh T., Galmandakh Z., Erdenebaatar J., Konnai S., Onuma M., Ohashi K.
  • Journal Title: J.Vet.Med.Sci. 70 Pages: 197-201
  • NAID: 110006613926
  • Peer Reviewed
• [Journal Article] A recombinant avian leukosis virus associated with fowl glioma in layer chickens in Japan (2008)
  • Author(s): Hatai H., Ochiai K., Nagakura K., Imanishi S., Ochi A., Kozakura R., Ono M., Goryo M., Ohashi K., Umemura T.
  • Journal Title: Avian Pathol. 37 Pages: 127-137
  • Peer Reviewed
• [Journal Article] Prevalence of fowl glioma-inducing virus in chickens of zoological gardens in Japan and nucleotide variation in the env gene (2008)
  • Author(s): Hatai H., Ochiai K., Murakami M., Imanishi S., Tomioka Y., Toyoda T., Ohashi K., Umemura T.
  • Journal Title: J.Vet.Med.Sci. 70 Pages: 469-474
  • NAID: 110006688951
  • Peer Reviewed
• [Journal Article] Serological survey of Toxoplasma gondii in wild waterfowl in chukotka, Kamchatka, Russia and Hokkaido, Japan (2008)
  • Author(s): Murao T., Omata Y., Kano R., Murata S., Okada T., Konnai S., Asakawa M., Ohashi K., Onuma M.
  • Journal Title: J.Parasitol. 94 Pages: 830-833
  • Peer Reviewed
• [Presentation] マレック病ウイルス感染鶏における免疫抑制受容体PD-1とそのリガンドPD-L1の発現解析 (2010)
  • Author(s): 河東あゆ美、村田史郎、加納里佳、今内覚、大橋和彦
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広畜産大学(帯広)
  • Year and Date: 2010-09-18
• [Presentation] マガンおよび鶏におけるinterferon-γ遺伝子プロモーター活性の比較解析 (2010)
  • Author(s): 松原綾子、村田史郎、加納里佳、橋口知幸、伊勢崎政美、河東あゆ美、小沼操、今内覚、大橋和彦
  • Organizer: 第150回 日本獣医学会学術集会
  • Place of Presentation: 帯広畜産大学(帯広)
  • Year and Date: 2010-09-18
• [Presentation] マレック病ウイルス感染鶏における免疫抑制受容体PD-1とそのリガンドPD-L1の発現解析 (2010)
  • Author(s): 河東あゆ美, ら
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広市・帯広畜産大学
  • Year and Date: 2010-09-18
• [Presentation] マガンなどの野生水禽の疾病抵抗性とマレック病ウイルスの分布 (2010)
  • Author(s): 大橋和彦、松原綾子、村田史郎、今内覚
  • Organizer: 第149回 日本獣医学会学術集会
  • Place of Presentation: 日本獣医生命科学大学(武蔵野)
  • Year and Date: 2010-03-28
• [Presentation] マガンなどの野生水禽の疾病抵抗性とマレック病ウイルスの分布 (2010)
  • Author(s): 大橋和彦, ら
  • Organizer: 第149回日本獣医学会学術集会
  • Place of Presentation: 武蔵野市・日本獣医生命科学大学
  • Year and Date: 2010-03-28
• [Presentation] Regulation of the interferon gamma expression by an onocoprotein of Marek's disease virus in wild waterfowls and chickens (2010)
  • Author(s): 松原綾子, 村田史郎, 加納里佳, 橋口知幸, 伊勢崎政美, 河東あゆ美, 小沼操, 今内覚, 大橋和彦
  • Organizer: 9th International Veterinary Immunology symposium
  • Place of Presentation: Tokyo, Japan
• [Presentation] 弱毒および強毒マレック病ウイルス感染鶏における免疫応答に関する研究 (2009)
  • Author(s): 加納里佳、今内覚、小沼操、大橋和彦
  • Organizer: 第7回サイトカイン研究会学術集会
  • Place of Presentation: とりぎん文化会館(鳥取)
  • Year and Date: 2009-09-24
• [Presentation] マレック病ウイルスmeq遺伝子の多型に伴うMeqタンパクの転写活性化能の変化 (2008)
  • Author(s): 橋口知幸、村田史郎、岡田宰、加納里佳、今内覚、小沼操、大橋和彦
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: シーガイア(宮崎)
  • Year and Date: 2008-09-24
• [Presentation] マレック病ウイルスmeq遺伝子の多型に伴うMeqタンパクの転写活性化能の変化 (2008)
  • Author(s): 橋口知幸ら
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎市・ワールドコンベンションセンターサミット
  • Year and Date: 2008-09-24
• [Presentation] Amino acid substitutions or insertion in the Meq proteins could affect their transactivation and transformation abilities (2008)
  • Author(s): Murata S., Okada T., Kano R., Hayashi Y., Hashiguchi T., Konnai S., Onuma M., Ohashi K.
  • Organizer: 8th International Marek's disease symposium
  • Place of Presentation: Townsvillie, Australia
• [Presentation] Expression of genes related to cell-mediated immune responses in chickens infected with Marek's disease virus (2008)
  • Author(s): 加納里佳, 村田史郎, 岡田宰, 今内覚, 小沼操, 大橋和彦
  • Organizer: 8th International Marek's disease symposium
  • Place of Presentation: Townsville, Australia
• [Presentation] Analysis of chicken p63 genein chicken lymphoblastoid tumor cell lines (2008)
  • Author(s): Kokubu R., Yamagishi N., Okada T., Ohashi K., Takagi M.
  • Organizer: 8th International Marek's disease symposium
  • Place of Presentation: Townsvi lie, Australia
• [Book] Animal Viruses(Maeda, A.(ed))(The detection and characterization of the meq variants of Marek's disease virus ) (2010)
  • Author(s): Ohashi K., Murata S., Okada T., Konnai S.
  • Publisher: Transworld Research Network, Kerala, India