| Project/Area Number |
20380159
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Iwate University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KIZAKI Keiichiro 岩手大学, 農学部, 准教授 (40337994)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKAHASHI Toru 農業生物資源研究所, 生殖機構ユニット, 主任研究員 (20355738)
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| Research Collaborator |
IMAI Kei 家畜改良センター, 技術部 (70343994)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2010: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2008: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Keywords | 細胞 / 組織 / 生体分子 / 遺伝子 / 発現制御 / 細胞・組織 / マイクロアレイ |
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| Research Abstract |
The purpose of this study is to raise the trophoblastic cell lines from in vitro fertilized embryonic cells for analyzing the mechanism of bovine implantation and placentogenesis. In exploring these mechanisms, trophoblastic cell lines can provide substantial information. Unfortunately, there are few cell lines for this purpose in cattle because of the difficulty of raising successive cell stock in the long term. In this study, 12 new cell lines were established using bone morphogenetic protein 4 (BMP4). These cell lines were maintained for around 30 passages, and they retained trophoblastic characteristics and expressed bovine trophoblastic genes: placental lactogen, interferon-τ, pregnancy-associated glycoprotein 1, and prolactin-related protein 1. Although the gene expression patterns were different among cell lines and depended on the cells, there were at least four characteristics. All of them expressed bovine POU domain class 5 transcription factor 1 and caudal-type home box 2. The expression of these genes was confirmed by quantitative RT-PCR and immunohistochemical detection. These results suggest that BMP4 is involved in the raising of trophoblast cell lines from early embryonic cells and the newly developed cell lines can provide different types of bovine trophoblastic cells with different cell lineages. This may constitute a significant new tool for the examination of trophoblastic differentiation.
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