• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Establishment of bovine trophoblastic cell lines

Research Project

Project/Area Number 20380159
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionIwate University

Principal Investigator

HASHIZUME Kazuyoshi  岩手大学, 農学部, 教授 (10355737)

Co-Investigator(Kenkyū-buntansha) KIZAKI Keiichiro  岩手大学, 農学部, 准教授 (40337994)
Co-Investigator(Renkei-kenkyūsha) TAKAHASHI Toru  農業生物資源研究所, 生殖機構ユニット, 主任研究員 (20355738)
Research Collaborator IMAI Kei  家畜改良センター, 技術部 (70343994)
Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2010: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2008: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Keywords細胞 / 組織 / 生体分子 / 遺伝子 / 発現制御 / 細胞・組織 / マイクロアレイ
Research Abstract

The purpose of this study is to raise the trophoblastic cell lines from in vitro fertilized embryonic cells for analyzing the mechanism of bovine implantation and placentogenesis. In exploring these mechanisms, trophoblastic cell lines can provide substantial information. Unfortunately, there are few cell lines for this purpose in cattle because of the difficulty of raising successive cell stock in the long term. In this study, 12 new cell lines were established using bone morphogenetic protein 4 (BMP4). These cell lines were maintained for around 30 passages, and they retained trophoblastic characteristics and expressed bovine trophoblastic genes: placental lactogen, interferon-τ, pregnancy-associated glycoprotein 1, and prolactin-related protein 1. Although the gene expression patterns were different among cell lines and depended on the cells, there were at least four characteristics. All of them expressed bovine POU domain class 5 transcription factor 1 and caudal-type home box 2. The expression of these genes was confirmed by quantitative RT-PCR and immunohistochemical detection. These results suggest that BMP4 is involved in the raising of trophoblast cell lines from early embryonic cells and the newly developed cell lines can provide different types of bovine trophoblastic cells with different cell lineages. This may constitute a significant new tool for the examination of trophoblastic differentiation.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (58 results)

All 2011 2010 2009 2008

All Journal Article (19 results) (of which Peer Reviewed: 17 results) Presentation (39 results)

  • [Journal Article] 着床とプロラクチン-ウシPRL/GHファミリーは着床誘導物質か?-2011

    • Author(s)
      橋爪一善、高橋透、木崎景一
    • Journal Title

      Hormone Frontier in Gynecolog

      Volume: 18 Pages: 31-38

    • Related Report
      2010 Final Research Report
  • [Journal Article] Bone morphogenetic protein 4 accelerates the establishment of bovine trophoblastic cell line2011

    • Author(s)
      Suzuki Y, Koshi K, Imai K, Takahashi T, Kizaki K, Hashizume
    • Journal Title

      Reproductio

      Volume: 142 Pages: 733-43

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of novel endogenous betaretroviruses which are transcribed in bovine placent2011

    • Author(s)
      Baba K, Nakaya Y, Shojima T, Muroi Y, Kizaki K, Hashizume K, Imakawa K, Miyazawa
    • Journal Title

      J Virolog

      Volume: 85 Pages: 1237-1245

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interactions between canine RAD51 and full length or truncated BRCA2 BRC repeat2011

    • Author(s)
      Ochiai K, Yoshikawa Y, Oonuma T, Tomioka Y, Hashizume K, Morimatsu
    • Journal Title

      Veterinary

      Volume: 190 Pages: 293-295

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD52011

    • Author(s)
      Ochiai K, Yoshikawa Y, Yoshimatsu K, Oonuma T, Tomioka Y, Takeda E, Arikawa J, Mominoki K, Omi T, Hashizume K, Morimatsu
    • Journal Title

      FEBS Let

      Volume: 585 Pages: 1771-1777

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of endogenous retrovirus-like transcripts in bovine trophoblastic cell2011

    • Author(s)
      Koshi K, Ushizawa K, Kizaki K, Takahashi T, Hashizume
    • Journal Title

      Placent

      Volume: 32 Pages: 493-9

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of novel endogenous betaretroviruses which are transcribed in bovine placenta2011

    • Author(s)
      Baba K, Nakaya Y, Shojima T, Muroi Y, Kizaki K, Hashizume K, Imakawa K, Miyazawa T
    • Journal Title

      J Virology

      Volume: 85 Pages: 1237-1245

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interactions between canine RAD51 and full length or truncated BRCA2 BRC repeats2011

    • Author(s)
      Ochiai K, Yoshikawa Y, Oonuma T, Tomioka Y, Hashizume K, Morimatsu M
    • Journal Title

      Veterinary J

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transforming growth factor beta family expression at the bovine feto-maternal interfac2010

    • Author(s)
      Sugawara K, Kizaki K, Herath CB, Hasegawa Y, Hashizume
    • Journal Title

      Reprod Biol Endocrino

      Volume: 8 Pages: 120-120

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and its related extracellular matrix degrading enzymes in the endometrium during estrous cycle and early gestation in cattle2010

    • Author(s)
      Mishra B, Kizaki K, Koshi K, Ushizawa K, Takahashi T, Hosoe M, Sato T, Ito A, Hashizume
    • Journal Title

      Reprod Biol Endocrino

      Volume: 8 Pages: 60-60

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cloning and expression of SOLD1 in ovine and caprine placenta, and their expected roles during the development of placentome2010

    • Author(s)
      Ushizawa K, Takahashi T, Hosoe M, Kizaki K, Hashizume
    • Journal Title

      BMC Dev Bio

      Volume: 10 Pages: 9-9

    • Related Report
      2010 Final Research Report
  • [Journal Article] Cleaved bovine prolactin-related protein-I stimulates vascular endothelial cell proliferatio2010

    • Author(s)
      Ushizawa K, Takahashi T, Hosoe M, Kizaki K, Hashizume
    • Journal Title

      Mol Cell Endocrino

      Volume: 323 Pages: 277-281

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transforming growth factor beta family expression at the bovine feto-maternal interface2010

    • Author(s)
      Sugawara K, Kizaki K, Herath CB, Hasegawa Y, Hashizume K
    • Journal Title

      Reprod Biol Endocrinol

      Volume: 8(電子雑誌) Pages: 120-120

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and its related extracellular matrix degrading enzymes in the endometrium during estrous cycle and early gestation in cattle2010

    • Author(s)
      Mishra B, Kizaki K, Koshi K, Ushizawa K, Takahashi T, Hosoe M, Sato T, Ito A, Hashizume K
    • Journal Title

      Reprod Biol Endocrinol

      Volume: 8(電子雑誌) Pages: 60-60

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cleaved bovine prolactin-related protein-I stimulates vascular endothelial cell proliferation2010

    • Author(s)
      Ushizawa K, Takahashi T, Hosoe M, Kizaki K, Hashizume K
    • Journal Title

      Mol Cell Endocrinol

      Volume: 323 Pages: 277-281

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cloning and expression of SOLD1 in ovine and caprine placenta, and their expected roles during the development of placentomes2010

    • Author(s)
      Ushizawa K, Takahashi T, Hosoe M, Kizaki K, Hashizume K.
    • Journal Title

      BMC Developmental Biology 10

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization and Expression Analysis of SOLD1, a Novel Member of the Retrotransposon-Derived Ly-6 Superfamily, in Bovine Placental Villi.2009

    • Author(s)
      Ushizawa K, Takahashi T, Hosoe M, Kizaki K, Hashizume K
    • Journal Title

      PLoS ONE 4(6)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Induction of Endogenous Tau Interferon Gene Transcription by CDX2 and High Acetylation in Bovine Non-Trophoblast Cells.2009

    • Author(s)
      Sakurai T, Sakamoto A, Muroi Y, Bai H, Nagaoka K, Tamura K, Takahashi T, Hashizume K, Sakatani M, Takahashi M, Godkin JD, Imakawa K
    • Journal Title

      Biol Reprod. 80

      Pages: 1223-1231

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The characterization of DNA methylation-mediated regulation of bovine placental lactogen and bovine prolactin-related prot ein-1 genes2009

    • Author(s)
      Nakaya, Y., Kizaki, K., Takah ashi, T., Patel, O.V., Hashizu me, K.
    • Journal Title

      BMC Mol Biol 10

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Differential cleavage of Bucentaur family members, intrinsically disordered/unstructured proteins, in bovine placentom2010

    • Author(s)
      Iwashita S, Nakashima K, Ohno-Iwashita Y, Kizaki K, Hashizume K, Imajoh-Ohmi S, Song S-Y
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
    • Related Report
      2010 Final Research Report
  • [Presentation] Differential cleavage of Bucentaur family members, intrinsically disordered/unstructured proteins, in bovine placentome2010

    • Author(s)
      Iwashita S, Nakashima K, Ohno-Iwashita Y, Kizaki K, Hashizume K, Imajoh-Ohmi S, Song S-Y
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Functional characterizations and expressional profiles of bovine endogenous retrovirus envelope proteins2010

    • Author(s)
      Nakaya Y, Koshi K, Kizaki K, Baba K, Imakawa K, Hashizume K, Miyazawa T
    • Organizer
      The 22nd Workshop on Retroviral Pathogenesis
    • Place of Presentation
      California(USA)
    • Year and Date
      2010-11-21
    • Related Report
      2010 Final Research Report
  • [Presentation] Functional characterizations and expressional profiles of bovine endogenous retrovirus envelope proteins2010

    • Author(s)
      Nakaya Y, Koshi K, Kizaki K, Baba K, Imakawa K, Hashizume K, Miyazawa T
    • Organizer
      The 22nd Workshop on Retroviral Pathogenesis
    • Place of Presentation
      California (USA)
    • Year and Date
      2010-11-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] ウシ内在性レトロウイルスのエンベロープタンパク質の発現と機能解析2010

    • Author(s)
      仲屋友喜、越勝男、木崎景一郎、馬場健司、今川和彦、橋爪一善、宮沢孝幸
    • Organizer
      第58回日本ウイルス学会学術集会
    • Place of Presentation
      徳島
    • Year and Date
      2010-11-08
    • Related Report
      2010 Final Research Report
  • [Presentation] ウシ内在性レトロウイルスのエンベロープタンパク質の発現と機能解析2010

    • Author(s)
      仲屋友喜、越勝男、木崎景一郎、馬場健司、今川和彦、橋爪一善、宮沢孝幸
    • Organizer
      第58回日本ウイルス学会学術集会
    • Place of Presentation
      徳島あわぎんホール
    • Year and Date
      2010-11-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] イヌ乳腺腫瘍関連遺伝子Brca2のBRC repeat4のアミノ酸配列がRad51との相互作用に及ぼす影響2010

    • Author(s)
      落合和彦、吉川泰永、大沼俊名、橋爪一善、森松正美
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広
    • Year and Date
      2010-09-16
    • Related Report
      2010 Final Research Report
  • [Presentation] ウシ内在性レトロウイルスエンベロープ遺伝子の胎盤形成に果たす役割2010

    • Author(s)
      越勝男、牛澤浩一、細江実佐、高橋透、木崎景一郎、橋爪一善
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広
    • Year and Date
      2010-09-16
    • Related Report
      2010 Final Research Report
  • [Presentation] ウシ子宮内膜におけるT細胞の動態と細胞障害性因子の発現解析2010

    • Author(s)
      大田友和、牛澤浩一、細江実佐、高橋透、山口高弘、木崎景一郎、橋爪一善
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広
    • Year and Date
      2010-09-16
    • Related Report
      2010 Final Research Report
  • [Presentation] イヌ乳腺腫瘍関連遺伝子Brca2のBRC repeat4のアミノ酸配列がRad51との相互作用に及ぼす影響2010

    • Author(s)
      落合和彦, 吉川泰永, 大沼俊名, 橋爪一善, 森松正美
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広畜産大学
    • Year and Date
      2010-09-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] ウシ内在性レトロウイルスエンベロープ遺伝子の胎盤形成に果たす役割2010

    • Author(s)
      越勝男, 牛澤浩一, 細江実佐, 高橋透, 木崎景一郎, 橋爪一善
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広畜産大学
    • Year and Date
      2010-09-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] ウシ子宮内膜におけるT細胞の動態と細胞障害性因子の発現解析2010

    • Author(s)
      大田友和, 牛澤浩一, 細江実佐, 高橋透, 山口高弘, 木崎景一郎, 橋爪一善
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広畜産大学
    • Year and Date
      2010-09-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Expression of ADAMTS-1 in the cyclic and pregnant bovine endometrium2010

    • Author(s)
      Mishra B, Kizaki K, Ushizawa K, Takahashi T, Hosoe M, Sato T, Ito A, Hashizume K
    • Organizer
      第103回日本繁殖生物学会大会
    • Place of Presentation
      十和田
    • Year and Date
      2010-09-04
    • Related Report
      2010 Final Research Report
  • [Presentation] Expression of ADAMTS-1 in the cyclic and pregnant bovine endometrium2010

    • Author(s)
      Mishra B, Kizaki K, Ushizawa K, Takahashi T, Hosoe M, Sato T, Ito A, Hashizume K
    • Organizer
      第103回日本繁殖生物学会大会
    • Place of Presentation
      十和田北里大学
    • Year and Date
      2010-09-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] ウシ子宮内膜間質細胞の遺伝子発現動態に及ぼす培養基質の影響2010

    • Author(s)
      木村美貴、木崎景一郎、橋爪一善
    • Organizer
      第103回日本繁殖生物学会大会
    • Place of Presentation
      十和田
    • Year and Date
      2010-09-02
    • Related Report
      2010 Final Research Report
  • [Presentation] ウシ胎盤における内在性レトロウイルスエンベロープ遺伝子の発現解析2010

    • Author(s)
      越 勝男、牛澤浩一、細江実佐、高橋透、木崎景一郎、橋爪一善
    • Organizer
      第103回日本繁殖生物学会大会
    • Place of Presentation
      十和田
    • Year and Date
      2010-09-02
    • Related Report
      2010 Final Research Report
  • [Presentation] ウシ子宮内膜間質細胞の遺伝子発現動態に及ぼす培養基質の影響2010

    • Author(s)
      木村美貴, 木崎景一郎, 橋爪一善
    • Organizer
      第103回日本繁殖生物学会大会
    • Place of Presentation
      十和田北里大学
    • Year and Date
      2010-09-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] ウシ胎盤における内在性レトロウイルスエンベロープ遺伝子の発現解析2010

    • Author(s)
      越勝男, 牛澤浩一, 細江実佐, 高橋透, 木崎景一郎, 橋爪一善
    • Organizer
      第103回日本繁殖生物学会大会
    • Place of Presentation
      十和田北里大学
    • Year and Date
      2010-09-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Active roles of heparin-biding EGF-like groth factor and its receptor in bovine endometrium during implantation2010

    • Author(s)
      Kizaki K, Kimura M, Iga K, Takahashi T, Hashizume K
    • Organizer
      Soc. for the Study of Reprod. 43rd Ann. Meeting
    • Place of Presentation
      Milwaukee (USA)
    • Year and Date
      2010-08-02
    • Related Report
      2010 Final Research Report
  • [Presentation] Expression of the endogenous retrovirus-like transcripts in bovine placenta2010

    • Author(s)
      Koshi K, Ushizawa K, Takahashi T, Kizaki K, Hashizume K
    • Organizer
      Soc. for the Study of Reprod. 43rd Ann. Meeting
    • Place of Presentation
      Milwaukee (USA)
    • Year and Date
      2010-08-02
    • Related Report
      2010 Final Research Report
  • [Presentation] Active roles of heparin-biding EGF-like groth factor and its receptor in bovine endometrium during implantation2010

    • Author(s)
      Kizaki K, Kimura M, Iga K, Takahashi T, Hashizume K
    • Organizer
      Soc.for the Study of Reprod.43rd Ann.Meeting
    • Place of Presentation
      Milwaukee (USA)
    • Year and Date
      2010-08-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Specific characters of new established bovine trophoblastic cell lines2010

    • Author(s)
      Hashizume K, Suzuki Y, Imai K, Takahashi T, Kizaki K
    • Organizer
      Soc. for the Study of Reprod. 43rd Ann. Meeting
    • Place of Presentation
      Milwaukee (USA)
    • Year and Date
      2010-08-01
    • Related Report
      2010 Final Research Report
  • [Presentation] Differential expression of EMMPRIN regulates endometrial remodeling during early gestation in the cow2010

    • Author(s)
      Mishra B, Kizaki K, Koshi K, Ushizawa K, Takahashi T, Hosoe M, Sato T, Ito A, Hashizume K
    • Organizer
      Soc. for the Study of Reprod. 43rd Ann. Meeting
    • Place of Presentation
      Milwaukee (USA)
    • Year and Date
      2010-08-01
    • Related Report
      2010 Final Research Report
  • [Presentation] Specific characters of new established bovine trophoblastic cell lines2010

    • Author(s)
      Hashizume K, Suzuki Y, Imai K, Takahashi T, Kizaki K
    • Organizer
      Soc.for the Study of Reprod.43rd Ann.Meeting
    • Place of Presentation
      Milwaukee (USA)
    • Year and Date
      2010-08-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] Expression of the endogenous retrovirus-like transcripts in bovine placenta2010

    • Author(s)
      Koshi K, Ushizawa K, Takahashi T, Kizaki K, Hashizume K
    • Organizer
      Soc.for the Study of Reprod.43rd Ann. Meeting
    • Place of Presentation
      Milwaukee (USA)
    • Year and Date
      2010-08-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] Differential expression of EMMPRIN regulates endometrial remodeling during early gestation in the cow2010

    • Author(s)
      Mishra B, Kizaki K, Koshi K, Ushizawa K, Takahashi T, Hosoe M, Sato T, Ito A, Hashizume K
    • Organizer
      Soc.for the Study of Reprod.43rd Ann.Meeting
    • Place of Presentation
      Milwaukee (USA)
    • Year and Date
      2010-08-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] ウシ栄養膜細胞特異的遺伝子は胎子-母体胎盤の形成と着床を誘導する2010

    • Author(s)
      木崎景一郎, 高橋透, 牛澤浩一, 細江実佐, 今井敬, 橋爪一善
    • Organizer
      世界的な乳牛繁殖効率低下の原因と対策に関するシンポジウム(平成21年度 文部科学省科学研究費補助金(研究成果公開促進費)補助事業)
    • Place of Presentation
      ワールドコンベンションセンター(宮崎)
    • Year and Date
      2010-01-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] ウシ栄養膜細胞の細胞譜系検証の試み2009

    • Author(s)
      鈴木康規, 木崎景一郎, 古澤軌, 細江実佐, 高橋透, 橋爪一善
    • Organizer
      第148回日本獣医学会学術集会
    • Place of Presentation
      市民会館(鳥取)
    • Year and Date
      2009-09-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] レトロトランスポゾン構造を持った分泌型Ly-6ドメインタンパク質SOLD1のウシ胎盤における発現と特徴2009

    • Author(s)
      牛澤浩一, 高橋透, 細江実佐, 木崎景一郎, 橋爪一善
    • Organizer
      第102回日本繁殖生物学会大会
    • Place of Presentation
      近畿大学(奈良)
    • Year and Date
      2009-09-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] ウシ子宮内膜組織における免疫関連細胞傷害性遺伝子Granulysinの発現解析2009

    • Author(s)
      大田友和, 木崎景一郎, 橋爪一善
    • Organizer
      第102回日本繁殖生物学会大会
    • Place of Presentation
      近畿大学(奈良)
    • Year and Date
      2009-09-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] Isolation of binucleate trophoblastic cells from bovine placenta.2009

    • Author(s)
      Kizaki K, Tanaka Y, Suzuki Y, Furusawa T, Ushizawa K, Hosoe M, Takahashi T, Hashizume K.
    • Organizer
      SSR 42nd Annual Meeting
    • Place of Presentation
      David L Lawrence Convention Center(Pittsburgh)
    • Year and Date
      2009-07-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] Effects of BMP4 on the establishment of bovine trophoblast cell lines2009

    • Author(s)
      Hashizume K, Suzuki Y, Kizaki K, Imai K, Takahashi T, Hosoe M.
    • Organizer
      SSR 42nd Annual Meeting
    • Place of Presentation
      David L Lawrence Convention Center (Pittsburgh)
    • Year and Date
      2009-07-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] ウシ栄養膜細胞系樹立におよぼすBMP4の作用2009

    • Author(s)
      鈴木康規, 木崎景一郎, 今井敬, 高橋透, 橋爪一善
    • Organizer
      第147回日本獣医学会学術集会
    • Place of Presentation
      栃木県総合文化センター(宇都宮)
    • Year and Date
      2009-04-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] ウシ胎盤性ラクトジェン(bCSH1)およびウシプロラクチン関連タンパク質1(bPRP1)遺伝子の転写制御機構の解析2009

    • Author(s)
      仲屋友喜, 木崎景一郎, 高橋透, Patel Osman, 橋爪一善
    • Organizer
      日本畜産学会第110回大会
    • Place of Presentation
      日本大学・藤沢
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] ウシ伸長胚における遺伝子発現動態の解析2009

    • Author(s)
      木崎景一郎, 今井敬, 牛澤浩一, 細江実佐, 高橋透, 橋爪一善
    • Organizer
      日本畜産学会第110回大会
    • Place of Presentation
      日本大学・藤沢
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] ウシプロラクチン関連タンパク質1の発現制御におけるDNAメチル化の関与について2008

    • Author(s)
      仲屋友喜, 木崎景一郎, 高橋透, 橋爪一善
    • Organizer
      第146回日本獣医学会学術集会
    • Place of Presentation
      シーガイヤ・宮崎
    • Year and Date
      2008-09-24
    • Related Report
      2008 Annual Research Report
  • [Presentation] 新規ウシ栄養膜細胞系樹立の試みとその特徴2008

    • Author(s)
      鈴木康規, 木崎景一郎, 今井敬, 高橋透, 橋爪一善
    • Organizer
      第101回日本繁殖生物学会大会
    • Place of Presentation
      九州大学・福岡
    • Year and Date
      2008-09-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヒツジ栄養膜細胞における分化制御因子の検索2008

    • Author(s)
      木崎景一郎, Stterfield, C., Song, G., Simmons, R., 橋爪一善, Spellcer. T.
    • Organizer
      第101回日本繁殖生物学会大会
    • Place of Presentation
      九州大学・福岡
    • Year and Date
      2008-09-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] Global gene expression profiling and differentiating bovine tr ophoblast cells2008

    • Author(s)
      Hashizume, K., Kizaki, K., Us hizawa, K., Hosoe, M., Takah ashi, T.
    • Organizer
      15th International Congress on Biotechnolo ev in Animal Reproduction
    • Place of Presentation
      Bangladesh Agricultural U niversity, Mymensingh
    • Year and Date
      2008-08-06
    • Related Report
      2008 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi