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Immortalization of prion protein deficient cell lines and establishment of prion-susceptible susceptible cell lines by introduction of prion protein genes.

Research Project

Project/Area Number 20380166
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionThe University of Tokyo

Principal Investigator

ONODERA Takashi  The University of Tokyo, 大学院・農学生命科学研究科, 教授 (90012781)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2008: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Keywords免疫 / ウイルス / プリオン / 感染症 / マクロファージ / 細胞不死化 / BSE / スクレイピー / プリオン病 / プリオン遺伝子欠損 / プリオン遺伝子欠損マウス / 不死化遺伝子 / マウス / 骨髄細胞
Research Abstract

After prion infection, an abnormal isoform of prion protein (PrP (Sc)) converts the cellular isoform of prion protein (PrP (C)) into PrP(Sc). PrP (C)-to-PrP (Sc) conversion leads to PrP (Sc) accumulation and PrP (C) deficiency, contributing etiologically to induction of prion diseases. Presently, most of the diagnostic methods for prion diseases are dependent on PrP (Sc) detection. Highly sensitive/accurate specific detection of PrP (Sc) in many different samples is a prerequisite for attempts to develop reliable detection methods. Towards this goal, several methods have recently been developed to facilitate sensitive and precise detection of PrP (Sc), namely, protein misfolding cyclic amplification, conformation-dependent immunoassay, dissociation-enhanced lanthanide fluorescent immunoassay, capillary gel electrophoresis, fluorescence correlation spectroscopy, flow microbead immunoassay, etc. Additionally, functionally relevant prion-susceptible cell culture models that recognize the complexity of the mechanisms of prion infection have also been pursued, not only in relation to diagnosis, but also in relation to prion biology. Prion protein (PrP) gene-deficient neuronal cell lines that can clearly elucidate PrP (C) functions would contribute to understanding of the prion infection mechanism. In this review, we describe the trend in recent development of diagnostic methods and cell culture models for prion diseases and their potential applications in prion biology.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (24 results)

All 2011 2010 2009 2008 Other

All Journal Article (17 results) (of which Peer Reviewed: 16 results) Presentation (2 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Enhanced enteric invasion of scrapie into villous columnar epithelium via immunoglobulin.2010

    • Author(s)
      Ano Y, Onodera T, et al
    • Journal Title

      Int.J.Mol.Med. 26

      Pages: 845-851

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of phagocytic activity by prion protein (PrP) in PrP-deficient macrophage cells.2010

    • Author(s)
      Uraki R, Onodera T, et al
    • Journal Title

      Int.J.Mol.Med. 26

      Pages: 527-532

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neuronal oxidative stress from NADPH oxidase among microglial cells infected with encephalomyocarditis virus.2010

    • Author(s)
      Ano Y, Onodera T, et al
    • Journal Title

      Neurosci.Lett. 469

      Pages: 39-43

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neuronal oxidative stress from NADPH oxidase among microglial cellsi nfected with encephalomyocarditis virus.2010

    • Author(s)
      Ano Y, Onodera T, et al.
    • Journal Title

      Neurosci.Lett.

      Volume: 469 Pages: 39-43

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of phagocytic activity by prion protein (PrP) in PrP-deficient macrophage cells.2010

    • Author(s)
      Uraki R, Onodera T, et al.
    • Journal Title

      Int.J.Mol.Med.

      Volume: 26 Pages: 527-532

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced enteric invasion of scrapie into villous columnar epithelium via immunoglobulin.2010

    • Author(s)
      Ano Y, Onodera T, et al.
    • Journal Title

      Int.J.Mol.Med.

      Volume: 26 Pages: 845-851

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of cellular isoform of prion protein in the function of macrophages and dendritic cells.2009

    • Author(s)
      Nitta, K., Sakudo, A., Masuyama, J., Xue, G., Sugiura, K., Onodera, T.
    • Journal Title

      Protein Pept.Lett. 16

      Pages: 239-244

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Uptake and dynamics of infectious prion protein in the intestine.2009

    • Author(s)
      Ano, Y., Onodera, T., et al.
    • Journal Title

      Protein Pept.Lett. 16

      Pages: 397-404

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Updated prediction for the BSE epidemic in dairy cattle in Japan.2009

    • Author(s)
      Sugiura, K., Onodera, T., et al
    • Journal Title

      Prev.Vet.Med. 89

      Pages: 272-276

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Risk of induction of BSE into Japan by historical importation of Cattle from the United Kingdom.2009

    • Author(s)
      Sugiura, K., Onodera, T., et al.
    • Journal Title

      J.Vet.Med.Sci. 71

      Pages: 133-138

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      小野寺節、杉浦勝明
    • Journal Title

      プリオン病と遅発性ウイルス感染症(金原出版)

      Pages: 1-12

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Transient production of abnormal prion protein requires the octapeptide repeat region of prion protein (PrP) in PrP-deficient neuronal cell line.2008

    • Author(s)
      Sakudo, A., Wu, G.Y., Onodera, T., Ikuta, K.
    • Journal Title

      Biochem.Biophys.Res.Commun. 365

      Pages: 164-169

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Species-specific anti-apoptotic activity of cellular prion protein in a mouse PrP-deficient neuronal cell line transfected with mouse, hamster and bovine Prnp.2008

    • Author(s)
      Wu, G.Y., Nakajima, K., Ano, Y., Takeyama, N., Yukawa, M, Taniuchi, Y., Sakudo, A., Onodera, T.
    • Journal Title

      Neurosci.Lett. 466

      Pages: 11-15

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of a new glial cell line from hippocampus of prion protein gene-deficient mice.2008

    • Author(s)
      Nishimura, T., Sakudo, A., Xue, G., Ikuta, K., Yukawa, M., Sugiura, K., Onodera, T.
    • Journal Title

      Biochem.Biophys.Res.Commun. 377

      Pages: 1047-1050

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transient production of abnormal prion protein requires the octarepeat region of PrP in PrP-deficient neuronal cell line2008

    • Author(s)
      Sakudo, A., Onodera, T. et. Al
    • Journal Title

      Biochem. Biophys. Res. Commun. 365

      Pages: 164-169

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cellular prion protein prevents brain damage after EMCV infection in mice.2008

    • Author(s)
      Nasu-Nishimura, Y., Onodera, T, et al.
    • Journal Title

      Arch. Virol. 153

      Pages: 1007-1012

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distinct immunohistochemical localization of Kuru plaques Using novel anti-PrP monoclonal antibodies.2008

    • Author(s)
      Hosokawa, T., and Onodera, T., etal.
    • Journal Title

      Microbiol. Immunol. 52

      Pages: 25-29

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] スクレイピー感染マウス脳における酸化ストレス障害2009

    • Author(s)
      阿野泰久、作道章一、梶村佳史、生田和良、小野寺節
    • Organizer
      第147回日本獣医学会学術集会
    • Place of Presentation
      栃木県宇都宮
    • Year and Date
      2009-04-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] 離乳移行期における経口投与アミロイド蛋白の腸管での取り込み2008

    • Author(s)
      阿野泰久、小野寺節、他
    • Organizer
      第145回日本獣医学会学術集会
    • Place of Presentation
      麻布大学
    • Year and Date
      2008-04-02
    • Related Report
      2008 Annual Research Report
  • [Book] 杉浦勝明食品におけるプリオン汚染リスク(食の安全科学の展開)シーエムシー2011

    • Author(s)
      小野寺節
    • Total Pages
      22
    • Related Report
      2010 Final Research Report
  • [Book] 食品におけるプリオン汚染リスク(食の安全科学の展開)2010

    • Author(s)
      小野寺節、杉浦勝明
    • Total Pages
      9
    • Publisher
      シーエムシー
    • Related Report
      2010 Annual Research Report
  • [Remarks] ホームページ等 東京大学食の安全研究センター

    • URL

      http://www.frc.a.u-tokyo.ac.jp/list/list_bse.html

    • Related Report
      2010 Final Research Report
  • [Remarks] 東京大学食の安全研究センター

    • URL

      http://www.frc.a.u-tokyo.ac.jp/list/list_bse.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.vm.a.u-tokyo.ac.jp/immune/2009-pub.html

    • Related Report
      2009 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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