Immortalization of prion protein deficient cell lines and establishment of prion-susceptible susceptible cell lines by introduction of prion protein genes.
| Project/Area Number |
20380166
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied veterinary science
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
ONODERA Takashi The University of Tokyo, 大学院・農学生命科学研究科, 教授 (90012781)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2008: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
|
|---|
| Keywords | 免疫 / ウイルス / プリオン / 感染症 / マクロファージ / 細胞不死化 / BSE / スクレイピー / プリオン病 / プリオン遺伝子欠損 / プリオン遺伝子欠損マウス / 不死化遺伝子 / マウス / 骨髄細胞 |
|---|
| Research Abstract |
After prion infection, an abnormal isoform of prion protein (PrP (Sc)) converts the cellular isoform of prion protein (PrP (C)) into PrP(Sc). PrP (C)-to-PrP (Sc) conversion leads to PrP (Sc) accumulation and PrP (C) deficiency, contributing etiologically to induction of prion diseases. Presently, most of the diagnostic methods for prion diseases are dependent on PrP (Sc) detection. Highly sensitive/accurate specific detection of PrP (Sc) in many different samples is a prerequisite for attempts to develop reliable detection methods. Towards this goal, several methods have recently been developed to facilitate sensitive and precise detection of PrP (Sc), namely, protein misfolding cyclic amplification, conformation-dependent immunoassay, dissociation-enhanced lanthanide fluorescent immunoassay, capillary gel electrophoresis, fluorescence correlation spectroscopy, flow microbead immunoassay, etc. Additionally, functionally relevant prion-susceptible cell culture models that recognize the complexity of the mechanisms of prion infection have also been pursued, not only in relation to diagnosis, but also in relation to prion biology. Prion protein (PrP) gene-deficient neuronal cell lines that can clearly elucidate PrP (C) functions would contribute to understanding of the prion infection mechanism. In this review, we describe the trend in recent development of diagnostic methods and cell culture models for prion diseases and their potential applications in prion biology.
|
Report
(4 results)
Research Products
(24 results)
-
-
-
-
-
-
-
[Journal Article] Role of cellular isoform of prion protein in the function of macrophages and dendritic cells.2009
Author(s)
Nitta, K., Sakudo, A., Masuyama, J., Xue, G., Sugiura, K., Onodera, T.
-
Journal Title
Protein Pept.Lett. 16
Pages: 239-244
Related Report
Peer Reviewed
-
-
-
-
-
-
[Journal Article] Species-specific anti-apoptotic activity of cellular prion protein in a mouse PrP-deficient neuronal cell line transfected with mouse, hamster and bovine Prnp.2008
Author(s)
Wu, G.Y., Nakajima, K., Ano, Y., Takeyama, N., Yukawa, M, Taniuchi, Y., Sakudo, A., Onodera, T.
-
Journal Title
Neurosci.Lett. 466
Pages: 11-15
Related Report
Peer Reviewed
-
[Journal Article] Establishment of a new glial cell line from hippocampus of prion protein gene-deficient mice.2008
Author(s)
Nishimura, T., Sakudo, A., Xue, G., Ikuta, K., Yukawa, M., Sugiura, K., Onodera, T.
-
Journal Title
Biochem.Biophys.Res.Commun. 377
Pages: 1047-1050
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
