Identification of canine hair follicle stem cells, reconstruction Of hair follicle and its application to wound healing

• Project/Area Number: 20380171
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: IWASAKI Toshiroh Tokyo University of Agriculture and Technology, 大学院・農学研究院, 教授 (50262754)
• Co-Investigator(Kenkyū-buntansha): NISHIFUJI Koji 東京農工大学, 大学院・農学研究院, 准教授 (20365422) ; OHYAMA Manabu 慶応義塾大学, 医学研究科, 講師 (10255424)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2009: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2008: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
• Keywords: 犬 / 幹細胞 / 皮膚 / 毛包 / 創傷治癒

Research Abstract

This research project demonstrated that canine hair follicle stem cells are located in a bulge area of hair follicles and high proliferation potency in the hair follicle stem cells. Stem cell marker proteins, K15, follistatin were stained intensely in the bulge area and related genes such as SOX9, LHX2, TCF4 and NFATC1 were expressed in hair follicle cells of bulge area. In vivo transplant of canine hair follicle stem cells with dermal papilla cells to nude mice successfully reconstituted the hair follicle and its adnexa in nude mouse skin. These results may give a new insight not only to canine hair follicle biology but also to human medicine.

Research Products

• [Journal Article] Molecular biological and immunohistological characterization of canine dermal papilla cells and the evaluation of cutaneous conditions. (2011)
  • Author(s): Kobayashi T, Fujisawa A, Amagai A, Iwasaki T, Ohyama M
  • Journal Title: Veterinary Dermatology in press
  • Peer Reviewed
• [Journal Article] Molecular biological and immunohistopathological characterization of canine dermal papilla cells and the evaluation of cutaneous conditions (2011)
  • Author(s): Kobayashi T, Iwasaki T, Ohyama T, et al.
  • Journal Title: Veterinary Dermatology
  • Peer Reviewed
• [Journal Article] Canine follicle stem cell candidates reside in the bulge and share characteristic features with human bulge cells. (2010)
  • Author(s): Kobayashi T, Iwasaki T, Amagai M, Ohyama M
  • Journal Title: Journal of Investigative Dermatology 130 Pages: 1988-1995
  • Peer Reviewed
• [Journal Article] Canine follicle stem cell candidates reside in the bulge and share chars cteristic features with human bulge cells (2010)
  • Author(s): Kobayashi T, Iwasaki T, Ohyama, et al.
  • Journal Title: Journal of Investigative Dermatology Volume: 130 Pages: 1988-1995
  • Peer Reviewed
• [Journal Article] Canine follicle stem cell candidates reside in the bulge and share characteristic features with human bulge cells (2010)
  • Author(s): Kobayashi T, Iwasaki T, et al.
  • Journal Title: Journal of Investigative Dermatology (印刷中)
  • Peer Reviewed
• [Journal Article] Canine hair-follicle keratinocytes enriched with bulge cells have the highly proliferative characteristic of stem cells. (2009)
  • Author(s): Kobayashi T, Shimizu A, Nishifuji K, Amagai M, Iwasaki T, Ohyama M
  • Journal Title: Veterinary Dermatology 20 Pages: 338-346
  • Peer Reviewed
• [Journal Article] Canine hair follicle keratinocytes enriched with bulge cells have the highly proliferative characteristic of stem cells (2009)
  • Author(s): Kobayashi T, Iwasaki T, et al.
  • Journal Title: Veterinary Dermatology 20 Pages: 338-346
  • Peer Reviewed
• [Journal Article] Identification and isolation of highly proliferative canine hair follicle kera tinocytes enriched for bulge eelIs : promis ing candidates for epithelial stem cells (2009)
  • Author(s): Kobayashi T, Shimizu A, Iwasaki T et. al.
  • Journal Title: Veterinary Dermatology (印刷中)
  • Peer Reviewed
• [Presentation] Recent advances in canine hair follicle stem cell biology (2011)
  • Author(s): 小林哲郎, 岩崎利郎, 大山学
  • Organizer: 2011 Annual Meeting of Australian College of Veterinary Sciences.
  • Place of Presentation: Gold Coast, Australia
• [Presentation] 犬の毛包幹細胞の同定および生物学的特徴の解析 (2010)
  • Author(s): 小林哲郎、西藤公司、天谷雅之、岩崎利郎、大山学
  • Organizer: 第149回日本獣医学会学術集会シンポジウム:再生医療の最前線～獣医臨床応用に向けた課題
  • Place of Presentation: 東京
  • Year and Date: 2010-03-27
• [Presentation] 犬の毛包幹細胞の同定と創傷治癒への応用について (2010)
  • Author(s): 小林哲郎、岩崎利郎、大山学, ほか
  • Organizer: 第149回日本獣医学会
  • Place of Presentation: 日本獣医畜産大学、東京
  • Year and Date: 2010-03-27
• [Presentation] Canine bulge cells share intrinsic properties with human bulge cells and provide a novel model for human hair follicles stem cell research. (2010)
  • Author(s): Kobayashi T, Iwasaki T, Amagai M, Ohyama M
  • Organizer: 6^<th> World Congress for Hair Research
  • Place of Presentation: Cairns, Australis
• [Presentation] Canine bulge cells share intrinsic proterties with human bulge cells abd provide a novel model for human hair follicle stem cell research (2010)
  • Author(s): Kobayashi T, Iwasaki T, Amagai M, Ohyama M
  • Organizer: The 6^<th> World Congress of Hair Research
  • Place of Presentation: Cairns, Australia
• [Presentation] イヌの毛包幹細胞の同定および生物学的特徴の解析 (2009)
  • Author(s): 小林哲郎、岩崎利郎、大山学, ほか
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: とりぎん文化会館、鳥取
  • Year and Date: 2009-09-26
• [Presentation] 犬の毛包幹細胞の同定および生物学的特徴の解析 (2009)
  • Author(s): 小林哲郎、岩崎利郎、西藤公司、大山学
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取
• [Presentation] 犬毛包はヒト毛包幹細胞研究に有用である-バルジ細胞の類似性を中心に- (2008)
  • Author(s): 小林哲郎, 岩崎利郎, 天谷雅行, 大山学
  • Organizer: 第15回毛髪利学研究会
  • Place of Presentation: 福岡
  • Year and Date: 2008-11-29
• [Presentation] Identification and isolation of highly proliferat ivecanine hair follicle bulge cells : promising candidatefor stem cells (2008)
  • Author(s): Kobayashi T, Nishifuji K, Iwasaki T, 0yama M, et al
  • Organizer: The 6^<th> World Congress of Veterinary Dermato1ogy
  • Place of Presentation: 香港国際会議&展示場
  • Year and Date: 2008-11-19
• [Presentation] 犬毛包はヒト毛包幹細胞研究に有用である-バルジ細胞の類似性を中心に- (2008)
  • Author(s): 小林哲郎、岩崎利郎、天谷雅之、大山学
  • Organizer: 第15回毛髪科学研究会
  • Place of Presentation: 福岡
  • Year and Date: 2008-11-09
• [Presentation] Identification and isolation of highly proliferative canine hair follicle bulge cells : promising candidate for stem cells. (2008)
  • Author(s): Kobayashi T, Nishifuji K, Iwasaki T, Amagai M, Ohyama M
  • Organizer: The 6^<th> World Congress of Veterinary Dermatology
  • Place of Presentation: Hong Kong
• [Presentation] Identification of human bulge cell counterpart in canine hair follicles : A valuable material for stem cell investigation (2008)
  • Author(s): Kobayashi T, Iwasaki T, Nishifuji K, Amagai M, Ohyama M
  • Organizer: International Investigative Dermatology Meeting
  • Place of Presentation: Kyoto
• [Presentation] Identification of human bulge cell counterpart in canine hair follicle : A valuable material for stem cell inve stigation (2008)
  • Author(s): Kobayashi T, Iwasaki T, Nishifuji K, Amagai M, 0yama M, et, al.
  • Organizer: International Investigative Dermatology meeting
  • Place of Presentation: 京都国際会館