| Project/Area Number |
20380174
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Clinical veterinary science
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
INABA Toshio 大阪府立大学, 生命環境科学研究科, 教授 (00137241)
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| Co-Investigator(Kenkyū-buntansha) |
SHUGIURA Kikuya 大阪府立大学, 生命環境科学研究科, 准教授 (30171143)
HATOYA Shingo 大阪府立大学, 生命環境科学研究科, 助教 (40453138)
SAKURAGAWA Norio 北里大学, 医療衛生学部, 研究員 (70183374)
TORII Ryuzo 滋賀医科大学, 動物生命科学研究センター, 教授 (50106647)
TAMADA Hiromichi 大阪府立大学, 生命環境科学研究科, 教授 (10155252)
KAWATE Noritoshi 大阪府立大学, 生命環境科学研究科, 准教授 (80221901)
KIDA Kayoko 大阪府立大学, 生命環境科学研究科, 助教 (50405362)
TANI Hiroyuki 大阪府立大, 生命環境科学研究科, 准教授 (00305658)
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| Project Period (FY) |
2008 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2010: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2009: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2008: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | 獣医学 / トランスレーショナルリサーチ / バイオテクノロジー / 移植・再生医療 / 発生・分化 / ES細胞 / イヌ / iPS細胞 / 卵子 / 体外成熟 / 神経細胞 / 骨髄間質細胞 / 再生医療 / 体外受精 / 体外発育 / 胚盤胞期胚 / 神経幹細胞 / コラーゲン・ゲル / 三次元培養 |
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| Research Abstract |
The objective of this study was to establish embryonic stem(ES) cell lines from in vitro matured and fertilized dog oocytes for regenerative veterinary medicine, and the following results were obtained.(1) We successfully enhanced oocyte growth and maturation by controlling the induction of fertile oestrus in immature and dioestrous bitches.(2) The in vitro maturation rate of canine oocytes was improved by embedding and culturing in collagen gels.(3) Canine ES-like cell colonies were formed by culturing inner cell masses collected from blastocysts in uterine horns, and maintained their proliferation and morphology beyond passage 10.(2) Neurons could be induced simply from primate ES cells.(3) Bone marrow stromal cell transplantation caused no complications and could have beneficial effects on functional recovery of dogs with spinal cord injury, and further studies are required to apply canine ES cells for the treatment of these dogs.
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