Comprehensive study of fungal biosurfactant proteins that recruit polymer-degrading enzymes onto solid polymer-surfaces
| Project/Area Number |
20380175
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | Tohoku University |
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Principal Investigator |
ABE Keietsu 東北大学, 大学院・農学研究科, 教授 (50312624)
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| Co-Investigator(Kenkyū-buntansha) |
SANO Motoaki 金沢工業大学, ゲノム生物工学研究所, 講師 (80410299)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2008: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | バイオマス / 固体醗酵 / 界面活性蛋白質 / hydrophobin / バイオマ / 固体発酵 / 界面活性タンパク質 |
|---|
| Research Abstract |
hen the industrial fungus Aspergillus oryzae was grown on cellulose and keratin as solid polymer substrates, two small bio-surfactant proteins (Mw <20 kDa)that were attached to the solid surfaces were isolated. Two genes encoding the two proteins were cloned and highly expressed in A. oryzae. The two purified bio-surfactant proteins attached to the surface of cellulose recruited xylanase F3 (XynF3)which lacks a carbohydrate biding domain. In vitro, molecular interaction analysis revealed the two proteins immobilized on the solid surfaces possessed dissociation constants KD. 10μM order to XynF3. We also analyzed interaction of esterase with another surfactant protein RolA attached to the two solid materials
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Characterization of Aspergillus oryzae glycoside hydrolase family 43 beta-xylosidase expressed in Escherichia coli2010
Author(s)
Suzuki S, Fukuoka M, Ookuchi H, Sano M, Ozeki K, Nagayoshi E, Takii Y, Matsushita M, Tada S, Kusumoto K, Kashiwagi Y
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Journal Title
J.Bioscie.Bioeng
Volume: 109
Pages: 115-117
Related Report
Peer Reviewed
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[Journal Article] Utilization of Aspergillus oryzae actin gene promoter for novel reporter system exploring anti-microtubule and lipid metabolism compounds and the target genes2010
Author(s)
Marui J., Yoshimi A., Hagiwara D., Fujii-Watanabe Y., Oda K., Koike H., Tamano K., Ishii T., Sano M., Machida M., K.Abe
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Journal Title
Appl.Microbiol.Biotechnol.
Volume: 87
Pages: 1829-1840
Related Report
Peer Reviewed
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[Book] Novel Industrial Applications of Aspergillus oryzae Genomics. Aspergillus : Molecular Biology and Genomics, Chapter 102010
Author(s)
Abe K., K. Furukawa, T. Fujioka, D. Hagiwara, H. Maeda, J. Marui, O. Mizutani, T. Takahashi, A. Yoshimi, Y. Yamagata, K. Gomi, F. Hasegawa
Publisher
Caister Academic Press
Related Report
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[Book] "Novel Industrial Applications of Aspergillus oryzae Genomics."Aspergillus : Molecular Biology and Genomics, Chapter 10, pp.199-2272010
Author(s)
Abe K., K.Furukawa, T.Fujioka, D.Hagiwara, H.Maeda, J.Marui, O.Mizutani, T.Takahashi, A.Yoshimi, Y.Yamagata, K.Gomi, F.Hasegawa
Publisher
Caister Academic Press
Related Report
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