| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2008: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Research Abstract |
hen the industrial fungus Aspergillus oryzae was grown on cellulose and keratin as solid polymer substrates, two small bio-surfactant proteins (Mw <20 kDa)that were attached to the solid surfaces were isolated. Two genes encoding the two proteins were cloned and highly expressed in A. oryzae. The two purified bio-surfactant proteins attached to the surface of cellulose recruited xylanase F3 (XynF3)which lacks a carbohydrate biding domain. In vitro, molecular interaction analysis revealed the two proteins immobilized on the solid surfaces possessed dissociation constants KD. 10μM order to XynF3. We also analyzed interaction of esterase with another surfactant protein RolA attached to the two solid materials
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