| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2008: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
|
|---|
| Research Abstract |
Osmoprotectant glycinebetaine synthesis enzymes from choline to glycine betaine was known only in Chenopodiaceae (spinach, sugar beet and halophytes) , although glycinebetaine is synthesized and accumulated in several plant families. We tried to find out the glycinebetaine synthesis enzymes in Gramineae, one of the most important crop plants using barley as experimental plant. We succeeded to characterize barley novel colinemonooxygenase (HvCMO) and betaine aldehyde dehydrogenases (HvBADH1 and HvBADH2) and determined the intercellular localization of HvCMO (peroxisome), HvBADH1 (peroxisome) andHvBADH2 (cytosol). These findings are totally unique and different from those of the Chenopodiaceae glycinebetaine synthetic enzymes in chloroplasts. We also studied on a novel glycinebetaine/proline transporter gene expressed in the mestome sheath and lateral root cap cells in barley. This finding suggests that plants have complicated but impressive glycinebetaine long distance transport systems.
|
