Development of sensory genomics for evaluation of the molecular basis of taste response and palatability distinction
Project/Area Number |
20380183
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied molecular and cellular biology
|
Research Institution | The University of Tokyo |
Principal Investigator |
ABE Keiko The University of Tokyo, 大学院・農学生命科学研究科, 特任教授 (10151094)
|
Co-Investigator(Kenkyū-buntansha) |
OKADA Shinji 東京大学, 大学院・農学生命科学研究科, 助教 (50376563)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
|
Keywords | 味覚システム / トランスジェニック動物 / 味覚応答 / 味の嗜好性 / 味覚伝達路 / 味細胞系譜 |
Research Abstract |
Using mice with a WGA tracer introduced, we analyzed the genes expressions in taste cells, taste neurons and cerebral cells by transcriptome, and revealed the molecular properties of geniculate ganglion (GG), petrosal ganglion (PG), and nucleus of the solitary tract (NST) WGA-positive neurons transmitting from sweet/umami taste- and bitter taste-receiving cells. We also constructed transgenic medaka fish whose amino acid-receiving (T1Rs-positive) and bitter taste-receiving (T2Rs-positve) cells were made to have the WGA, and succeeded in visualizing their taste-transmitting pathways.
|
Report
(4 results)
Research Products
(156 results)
-
-
[Journal Article] The single pore residue Asp (523) in PKD2L1 deter-mines Ca^<2+> permeation of a PKD1L3/PKD2L1 complex.2011
Author(s)
Fujimoto, C., Ishimaru, Y., Katano, Y., Misaka, T., Yamasoba, T., Asakura, T., Abe, K.
-
Journal Title
Biochem.Biophys.Res.Commun. 404
Pages: 946-951
Related Report
-
[Journal Article] Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion : case of a nonglycosylated mutant of miraculin, a taste-modifying protein.2011
Author(s)
Ito, K., Sugawara, T., Koizumi, A., Nakajima, K.I., Shimizu-Ibuka, A., Shiroishi, M., Asada, H., Yurugi-Kobayashi, T., Shimamura, T., Asakura, T., Misaka, T., Iwata, S., Kobayashi, T., Abe, K.
-
Journal Title
Biotechnol.Lett. 33
Pages: 103-107
NAID
Related Report
-
-
-
-
[Journal Article] The human bitter taste receptor, hTAS2R16, discriminates slight differences in the configuration of disaccharides.2010
Author(s)
Sakurai, T., Misaka, T., Ueno, Y Ishiguro, M., Matsuo, S., Ishimaru, Y., Asakura, T., Abe, K.
-
Journal Title
Biochem.Biophys.Res.Commun. 402
Pages: 595-601
Related Report
-
[Journal Article] Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans.2010
Author(s)
Ito, K., Ito, S., Shimamura, T., Kawarasaki, Y., Abe, K., Misaka, T., Kobayashi, T., Iwata, S.
-
Journal Title
Acta Crystallogr.Sect.F Struct.Biol.Cryst.Commun. 66
Pages: 1086-1088
Related Report
-
[Journal Article] Surface plasmon resonance analysis on interactions of food components with a taste epithelial cell model.2010
Author(s)
Miyano, M., Yamashita, H., Sakurai, T., Nakajima, K., Ito, K., Misaka, T., Ishimaru, Y., Abe, K., Asakura, T.
-
Journal Title
J.Agric.Food Chem. 58
Pages: 11870-11875
Related Report
-
[Journal Article] Characterization of the beta-D-glucopyranoside binding site of the human bitter taste receptor hTAS2R16.2010
Author(s)
Sakurai, T., Misaka, T., Ishiguro, M., Masuda, K., Sugawara, T., Ito, K., Kobayashi, T., Matsuo, S., Ishimaru, Y., Asakura, T., Abe, K.
-
Journal Title
J.Biol.Chem. 285
Pages: 28373-28378
Related Report
-
[Journal Article] Interaction between PKD1L3 and PKD2L1 through their transmembrane domains is required for localization of PKD2L1 at taste pores in taste cells of circumvallate and foliate papillae.2010
Author(s)
Ishimaru, Y., Katano, Y., Yamamoto, K., Akiba, M., Misaka, T., Roberts, R.W., Asakura, T., Matsunami H., Abe, K.
-
Journal Title
FASEB J. 24
Pages: 4058-4067
Related Report
-
[Journal Article] Amiloride reduces the sweet taste intensity by inhibiting the human sweet taste receptor.2010
Author(s)
Imada, T., Misaka, T., Fujiwara, S., Okada, S., Fukuda, Y., Abe, K.
-
Journal Title
Biochem.Biophys.Res.Commun. 397
Pages: 220-225
Related Report
-
-
[Journal Article] Bulky high-mannose-type N-glycan blocks the taste-modifying activity of miraculin.2010
Author(s)
Ito, K., Sugawara, T., Koizumi, A., Nakajima, K., Shimizu-Ibuka, A., Shiroishi, M., Asada, H., Yurugi-Kobayashi, T., Shimamura, T., Asakura, T., Misaka, T., Iwata, S., Kobayashi, T., Abe, K.
-
Journal Title
Biochim.Biophys.Acta 1800
Pages: 986-992
Related Report
-
[Journal Article] Photoactive ligands probing the sweet taste receptor.Design and synthesis of highly potent diazirinyl d-phenylalanine derivatives.2010
Author(s)
Masuda, K., Koizumi, A., Misaka, T., Hatanaka, Y., Abe, K., Tanaka, T., Ishiguro, M., Hashimoto, M.
-
Journal Title
Bioorg.Med.Chem.Lett. 20
Pages: 1081-1083
Related Report
-
-
-
-
-
-
-
-
-
-
[Journal Article] The Hsp90 inhibitor geldanamycin abrogates colocalization of eIF4E and 4E-T into stress granules and association of eIF4E with eIF4G.2009
Author(s)
Suzuki, Y., Minami, M., Suzuki, M., Abe, K., Zenno, S., Tsujimoto, M., Matsumoto, K., Minami, Y.
-
Journal Title
J.Biol.Chem. 284
Pages: 35597-35604
Related Report
-
[Journal Article] pH-dependent structural change in neoculin with special reference to its taste-modifying activity.2009
Author(s)
Morita, Y., Nakajima, K., Iizuka, K., Terada, T., Shimizu-Ibuka, A., Ito, K., Koizumi, A., Asakura, T., Misaka, T., Abe, K.
-
Journal Title
Biosci.Biotechnol.Biochem. 73
Pages: 2552-2555
NAID
Related Report
-
[Journal Article] Spatial differences in molecular characteristics of the pontine parabrachial nucleus.2009
Author(s)
Maeda, N., Onimura, M., Ohmoto, M., Inui, T., Yamamoto, T., Matsumoto, I., Abe, K.
-
Journal Title
Brain Res. 1296
Pages: 24-34
Related Report
-
[Journal Article] Acetic acid activates PKD1L3-PKD2L1 channel-A candidate sour taste receptor.2009
Author(s)
Ishii, S., Misaka, T., Kishi, M., Kaga, T., Ishimaru, Y. Abe, K.
-
Journal Title
Biochem.Biophys.Res.Commun. 385
Pages: 346-350
Related Report
-
-
[Journal Article] Functional organization of the rodent parabrachial nucleus.2009
Author(s)
Yamamoto, T., Takemura, M., Inui, T., Torii, K., Maeda, N., Ohmoto, M., Matsumoto, I., Abe, K.
-
Journal Title
Ann.N.Y.Acad.Sci. 1170
Pages: 378-382
Related Report
-
-
[Journal Article] Flourescence- based optimization of human bitter taste receptor expression in Saccharomyces cerevisiae.2009
Author(s)
Sugawara, T., Ito, K., Shiroishi, M., Tokuda, N., Asada, H., Yurugi-Kobayashi, T., Shimamura, T., Misaka, T., Nomura, N., Murata, T., Abe, K., Iwata, S., Kobayashi, T.
-
Journal Title
Biochem.Biophys.Res.Commun. 382
Pages: 704-710
Related Report
-
-
[Journal Article] pH-Dependent inhibition of the human bitter taste receptor hTAS2R16 by a variety of acidic substances.2009
Author(s)
Sakurai, T., Misaka, T., Nagai, T., Ishimaru, Y., Matsuo, S., Asakura T., Abe, K.
-
Journal Title
J.Agric.Food Chem. 57
Pages: 2508-2514
Related Report
-
[Journal Article] Umami taste dysfunction in patients receiving radiotherapy for head and neck cancer.2009
Author(s)
Yamashita, H., Nakagawa, K., Hosoi, Y., Kurokawa, A., Fukuda, Y., Matsumoto, I., Misaka, T., Abe, K.
-
Journal Title
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Introduction2010
Author(s)
阿部啓子
Organizer
NNC Meeting at Nestle
Place of Presentation
Switzerland, Geneva.
Year and Date
2010-04-16
Related Report
-
[Presentation] Introduction2010
Author(s)
Keiko Abe
Organizer
NNC Meeting at Nestle
Place of Presentation
Switzerland, Geneva
Year and Date
2010-04-16
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-