Study of molecular mechanism of the ER quality control in plants

• Project/Area Number: 20380188
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Osaka Prefecture University
• Principal Investigator: KOIZUMI Nozomu Osaka Prefecture University, 生命環境科学研究科, 教授 (20252835)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000); Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2008: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: 小胞体ストレス / シロイヌナズナ / 転写因子 / スプライシング / シャペロン / 小胞体 / タンパク質の品質管理

Research Abstract

If proteins with abnormal structures accumulate in the intracellular compartment called the endoplasmic reticulum (ER), such condition is transmitted to the nucleus causing activation of genes for chaperones that assist the correct folding of proteins. We found that a sensor protein IRE1 localizing on the ER membrane is involved in cytoplasmic splicing as the first report of cytoplasmic splicing in plants. It was considered that a membrane bound transcription factor bZIP60 was converted to active form losing its transmembrane domain by the splicing and translocated to the nucleus activating target genes.

Research Products

• [Journal Article] 植物における小胞体の品質管理-病原菌認識機構の解析から見えてきたもの- (2011)
  • Author(s): 小泉望、岩田雄二
  • Journal Title: 化学と生物 49 Pages: 88-91
  • NAID: 10027898368
  • Peer Reviewed
• [Journal Article] Development of series of gateway binary vectors possessing a tunicamycin resistance gene as a marker for transformation of Arabidopsis thaliana. (2011)
  • Author(s): Tanaka, Y., Nakamaura, S., Kawamukai, M., Koizumi N., Nakagawa, T.
  • Journal Title: Bioscience, Biotechnology and Biochemistry in press
  • NAID: 10028272038
  • Peer Reviewed
• [Journal Article] De novo DNA methylation of the 35S enhancer revealed by high-resolution methylation analysis of an entire T-DNA segment in transgenicgentian. (2011)
  • Author(s): atoshi Yamasaki, Masayuki Oda, Nozomu Koizumi, Kazuhiko Mitsukuri, Masafumi Johkan, Takashi Nakatsuka, Masahiro Nishihara, Kei-ichiro Mishiba
  • Journal Title: Plant Biotechnology Volume: 28 Pages: 223-230
  • Peer Reviewed
• [Journal Article] 植物における小胞体の品質管理病原菌認識機構の解析から見えてきたもの (2011)
  • Author(s): 小泉望、岩田雄二
  • Journal Title: 化学と生物 Volume: 49 Pages: 88-91
  • NAID: 10027898368
• [Journal Article] Development of series of gateway binary vectors possessing a tunica-mycin resistance gene as a marker for transformation of Arabidopsis thaliana. (2011)
  • Author(s): Yuji TANAKA, Shinya NAKAMURA, Makoto KAWAMUKAI Nozomu KOIZUM3, Tsuyoshi NAKAGAWA
  • Journal Title: Bioscience, Biotechnology and Biochetis Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Characterization of a plant-specific gene Induced by endoplasmic reticulum stress in Arabidopsis thaliana. (2010)
  • Author(s): Iwata Y., Nishino T., Takayama S., Koizumi N.
  • Journal Title: Bioscience, Biotechnology and Biochemistry 74 Pages: 2087-2091
  • NAID: 10027560669
  • Peer Reviewed
• [Journal Article] Transcriptomic response of Arabidopsis thaliana to tunicamycin-induced endoplasmic reticulum stress. (2010)
  • Author(s): Iwata Y., Sakiyama, M., Lee M.H., Koizumi N.
  • Journal Title: Plant Biotechnology 27 Pages: 161-171
  • NAID: 10026527128
  • Peer Reviewed
• [Journal Article] Transcriptomic response of Arabidopsis thaliana to tunicamycin-induced endoplasmic reticulum stress. (2010)
  • Author(s): Yuji Iwata, Masayo Sakiyama, Mi-Hyun Lee, Nozomu Koizumi
  • Journal Title: Plant Biotechnology Volume: 27 Pages: 161-171
  • NAID: 10026527128
  • Peer Reviewed
• [Journal Article] Characterization of a plant-specific gene Induced by endoplasmic reticulum stress in Arabidopsis thaliana. (2010)
  • Author(s): Yuji Iwata, Tsuneyo Nishinio, Seiji Takayama, Nozomu Koizumi
  • Journal Title: Bioscience, Biotechnology and Biochemistry Volume: 74 Pages: 2087-2091
  • NAID: 10027560669
  • Peer Reviewed
• [Journal Article] 遺伝子組換え(GM)ナタネのこぼれ種と生物多様性 (2010)
  • Author(s): 小泉望、中山祐一郎
  • Journal Title: バイオサイエンスとインダストリー Volume: 68 Pages: 361-363
  • NAID: 10026587102
• [Journal Article] Characteristics of the nuclear form of the Arabidopsis transcription factor AtbZIP60 during the endoplasmic reticulum stress response. (2009)
  • Author(s): Iwata Y., Yoneda, M., Yanagawa, Y., Koizumi N.
  • Journal Title: Bioscience, Biotechnology and Biochemistry 73 Pages: 865-869
  • NAID: 10027539778
  • Peer Reviewed
• [Journal Article] The Arabidopsis membrane-bound transcription factor AtbZIP60 is a novel plant-specific endoplasmic reticulum stress transducer. (2009)
  • Author(s): Iwata Y., Fedoroff, N.V., Koizumi N.
  • Journal Title: Plant Signaling and Behavior 4 Pages: 514-516
  • Peer Reviewed
• [Journal Article] Characteristics of the nuclear form of the Arabidopsis transcription factor AtbZIP60 during the endoplasmic reticulum stress response. (2009)
  • Author(s): Iwata, Y., Yoneda, M., Yanagawa, Y., Koizumi, N
  • Journal Title: Bioscience, Biotechnology and Biochemistry 73 Pages: 865-869
  • NAID: 10027539778
  • Peer Reviewed
• [Journal Article] The Arabidopsis membrane-bound transcription factor AtbZIP60 is anovel plant-specific endoplasmic reticulum stress transducer. (2009)
  • Author(s): Iwata, Y., Fedoroff, N.V., Komumi, N.
  • Journal Title: Plant Signaling and Behavior 4 Pages: 514-516
  • Peer Reviewed
• [Journal Article] Construction of a binary vector for transformation of Arabidopsis thaliana with a new selection marker. (2008)
  • Author(s): 小泉望, 岩田雄二
  • Journal Title: Bioscience, Biotechnology and Biochemistry 72 Pages: 3041-3043
  • NAID: 10027534451
  • Peer Reviewed
• [Journal Article] Arabidopsis bZIP60 is a proteolysis-activated transcription factor involved in the endoplasmic reticulum stress response. (2008)
  • Author(s): Iwata Y., Fedoroff, N.V., Koizumi N.
  • Journal Title: Plant Cell 20 Pages: 3107-3121
  • Peer Reviewed
• [Journal Article] NtbZIP60, an endoplasmic reticulum-localized transcription factor, plays a role in the defense response against bacterial pathogens in tobacco. (2008)
  • Author(s): Tateda, C., Ozaki, R., Onodera, Y., Takahashi, Y., Yamaguchi, K., Berberich, T., Koizumi N., Kusano T.
  • Journal Title: Journal of Plant Research 121 Pages: 603-611
  • Peer Reviewed
• [Journal Article] Identification of an Arabidopsis transmembrane bZIP transcription factor involved in the endoplasmic reticulum stress response. (2008)
  • Author(s): Tajima, H., Iwata Y., Iwano, M., Takayama, S., Koizumi N.
  • Journal Title: Biochemical and Biophysical Research Communications 374 Pages: 242-247
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of an Arabidopsis gene encoding a CCT domain-containing protein during endoplasmic reticulum stress. (2008)
  • Author(s): Iwata Y., Yamada, T., Koizumi N.
  • Journal Title: Plant Biotechnology 25 Pages: 397-402
  • NAID: 10021914293
  • Peer Reviewed
• [Journal Article] Endoplasmic reticulum stress response and regulated intramembrane proteolysis in plants. (2008)
  • Author(s): Tajima, H., Iwata Y., Koizumi N.
  • Journal Title: Plant Biotechnology 25 Pages: 271-278
  • NAID: 10021913593
  • Peer Reviewed
• [Journal Article] The crystal structure of plant-specific calcium-binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14. (2008)
  • Author(s): Akaboshi, M., Hashimoto, H., Ishida, H., Saijo, S., Koizumi N., et al.
  • Journal Title: Journal of Molecular Biology 377 Pages: 246-257
  • Peer Reviewed
• [Journal Article] Arabidopsis bZIP60 is a proteolysis-activated transcription factor involved in the endoplasmic reticulum stress response (2008)
  • Author(s): Iwata Y., Fedoroff V. N., Koizumi N.
  • Journal Title: Plant Cell 20 Pages: 3107-3121
  • Peer Reviewed
• [Journal Article] Identification of an Arabidopsis transmembrane bZIP transcription factor involved in the endoplasmic reticulum stress response (2008)
  • Author(s): Tajima H., et al.
  • Journal Title: Biochem. Biophy. Res. Commun 374 Pages: 242-247
  • Peer Reviewed
• [Journal Article] Construction of a binary vector for transformation of Arabidopsis thaliana with a new selection marker. (2008)
  • Author(s): Koizumi N., Iwata Y.
  • Journal Title: Biosci. Biotech. Biochem 72 Pages: 3041-3043
  • NAID: 10027534451
  • Peer Reviewed
• [Presentation] ビアラフォス、ツニカマイシン選択が可能な植物形質転換用Gatewayバイナリーベクターシリーズの開発 (2011)
  • Author(s): 田中優史、中村真也、真野昌二、大西真人、川向誠、佐藤豊、石黒澄衛、小泉望、中川剛
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-26
• [Presentation] シロイヌナズナ膜結合型転写因子bZIP60のFumonisin B1による活性化 (2011)
  • Author(s): 長島幸広, 李美賢, 小泉望
  • Organizer: 日本植物生理学会
  • Place of Presentation: 仙台(宮城)
  • Year and Date: 2011-03-20
• [Presentation] The ER Stress Response in Arabidopsis, Molecular Farming : Promises and Challenges (2011)
  • Author(s): 小泉望
  • Organizer: Korean Society for Molecular Farming Research
  • Place of Presentation: 韓国ソウル
  • Year and Date: 2011-01-18
• [Presentation] The ER Stress Response in Arabidopsis (2011)
  • Author(s): 小泉望
  • Organizer: Molecular Farming : Promises and Challenges (Korean Society for Molecular Farming Research)
  • Place of Presentation: ソウル教育文化会館(韓国ソウル)
  • Year and Date: 2011-01-18
• [Presentation] Membrane-bound Transcription Factors involved in the Arabidopsis ER stress response (2010)
  • Author(s): 小泉望
  • Organizer: BMB2010(第33回日本分子生物学会年会/第83回日本生化学会大会合同大会)
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Membrane-bound Transcription Factors involved in the Arabidopsis ER stress response (2010)
  • Author(s): 小泉望
  • Organizer: BMB2010(第33回日本分子生物学会年会/第83回日本生化学会大会合同大会)
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2010-12-08
• [Presentation] Membrane-bound transcription factors in Arabidopsis (2010)
  • Author(s): 小泉望
  • Organizer: 奈良先端科学技術大学院大学グローバルCOEセミナー
  • Place of Presentation: 奈良先端科学技術大学院大学(生駒市)
  • Year and Date: 2010-11-19
• [Presentation] シロイヌナズナ膜結合型転写因子のFumonisin B1による活性化 (2010)
  • Author(s): 李美賢, 崎山雅代, 長島幸広, 小泉望
  • Organizer: 日本農芸化学関西支部大会
  • Place of Presentation: 奈良
  • Year and Date: 2010-10-03
• [Presentation] シロイヌナズナ膜結合型転写因子のFumonisin B1による活性化 (2010)
  • Author(s): 李美賢, 崎山雅代, 長島幸広, 小泉望
  • Organizer: 日本農芸化学会平成22年度関西支部大会(第466回講演会)
  • Place of Presentation: 近畿大学農学部(奈良市)
  • Year and Date: 2010-10-03
• [Presentation] An Arabidopsis transcription factor AtbZIP60 involved in the unfolded protein response also regulates genes induced by fumonisin B1 (2010)
  • Author(s): Mi-Hyun Lee, Masayo Sakiyama, Yukihiro Nagashima, Nozomu Koizumi
  • Organizer: 国際シンポジ ウム:タンパク質の社会
  • Place of Presentation: 奈良
  • Year and Date: 2010-09-13
• [Presentation] An Arabidopsis transcription factor AtbZIP60 involved in the unfolded protein response also regulates genes induced by fumonisin (2010)
  • Author(s): Mi-Hyun Lee, Masayo Sakiyama, Yukihiro Nagashima, Nozomu Koizumi
  • Organizer: 第3回国際シンポジウム「タンパク質の社会(Protein Community)
  • Place of Presentation: ホテル日航奈良(奈良市)
  • Year and Date: 2010-09-13
• [Presentation] シロイヌナズナの膜結合型転写因子bZIP60によるPDR12の制御 (2010)
  • Author(s): 李美賢、崎山雅代、長島幸広、小泉望
  • Organizer: 日本植物細胞分子生物学会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-03
• [Presentation] シロイヌナズナの膜結合型転写因子bZIP60によるPDR12の制御 (2010)
  • Author(s): 李美賢、崎山雅代、長島幸広、小泉望
  • Organizer: 第28回日本植物細胞分子生物学会(仙台)大会
  • Place of Presentation: 東北大学(仙台市)
  • Year and Date: 2010-09-03
• [Presentation] 小胞体ストレス応答を制御する転写因子のプログラム細胞死への関与 (2010)
  • Author(s): 李美賢, 小泉望
  • Organizer: 日本植物細胞分子生物学会
  • Place of Presentation: 湘南(神奈川)
  • Year and Date: 2010-07-30
• [Presentation] A membrane-bound transcription factor mediates two independent signaling pathways (2010)
  • Author(s): Mi-Hyun Lee, Yuji Iwata, Nozomu Koizumi
  • Organizer: 第21回国際シロイヌナズナ研究会議
  • Place of Presentation: 横浜
  • Year and Date: 2010-06-08
• [Presentation] Membrane-bound transcription factor mediates two independent signaling pathways (2010)
  • Author(s): Mi-Hyun Lee, Yuji Iwata, Nozomu Koizumi
  • Organizer: 21stICAR(第21回国際シロイヌナズナ研究会議)
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2010-06-08
• [Presentation] Membrane-bound transcription factor in Arabidopsis mediates two independent signaling (2010)
  • Author(s): 小泉望
  • Organizer: Crop Functional Genomics 2010(Korean Society for Plant Biotechnology)
  • Place of Presentation: 韓国済州島
  • Year and Date: 2010-04-16
• [Presentation] Membrane-bound transcription factor in Arabidopsis mediates two independent signaling (2010)
  • Author(s): 小泉望
  • Organizer: Crop Functional Genomics 2010 (Korean Society for Plant Biotechnology)
  • Place of Presentation: ラマダプラザチェジュ(韓国済州島)
  • Year and Date: 2010-04-16
• [Presentation] Regulation of the Arabidopsis ER stress response by membrane-bound bZIP transcription factors (2009)
  • Author(s): Nozomu Koizumi, Hiromi Tajima, Nina V Fedoroff, Yuji Iwata
  • Organizer: Plant Biology 2009
  • Place of Presentation: 米国ホノルル
  • Year and Date: 2009-07-20
• [Presentation] Regulation of the Arabidopsis ER stress response by membrane-bound bZIP transcription factors (2009)
  • Author(s): Nozomu Koizumi, Hiromi Tajima, Nina V Fedoroff, Yuji Iwata
  • Organizer: Plant Biology 2009
  • Place of Presentation: ホノルル(米国)
  • Year and Date: 2009-07-20
• [Presentation] シロイヌナズナの小胞体ストレス応答に関わる膜局在型転写因子 (2009)
  • Author(s): 小泉望, 田嶋紘美, 岩田雄二
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-23
• [Presentation] シロイヌナズナの小胞体ストレス応答に関わる膜局在型転写因子 (2009)
  • Author(s): 小泉 望, 田嶋紘美, 岩田雄二
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-23
• [Presentation] 小胞体ストレス応答遺伝子の花粉特異的発現 (2008)
  • Author(s): 西野恒代, 李美賢, 岩田雄二, 岩野恵, 高山誠司, 小泉望
  • Organizer: 日本植物細胞分子生物学会
  • Place of Presentation: 吹田(大阪)
  • Year and Date: 2008-09-02
• [Book] Analysis of a transcription factor using transient assay in Arabidopsis protoplasts, in Plant Transcription Factors(eds. L. Yuan & S.E. Perry)Vol.754 (2011)
  • Author(s): Iwata, Y., Lee, M.H., Koizumi, N.
  • Publisher: Humana Press in press
• [Book] Plant Transcription Factors(Methods in Molecular Biology, Vol.754) (2011)
  • Author(s): Yuji Iwata, Mi-Hyun Lee, Nozomu Koizumi
  • Total Pages: 310
  • Publisher: A Humana Press