| Project/Area Number |
20390009
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Tohoku University |
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Principal Investigator |
GOTO Junichi Tohoku University, 病院, 名誉教授 (80006337)
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| Co-Investigator(Kenkyū-buntansha) |
MANO Nariyasu 東北大学, 病院, 教授 (50323035)
SHIMADA Miki 東北大学, 病院, 准教授 (10196488)
YAMAGUCHI Hiroaki 東北大学, 病院, 助教 (80400373)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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| Keywords | 分析化学 / 機能性器材 / 脳 / トランスポーター / 胆汁酸 / プロテオーム / リン酸化タンパク質 / 結合タンパク質 / ケノデオキシコール酸 / アフィニティー抽出 / 疑似ニュートラルロス / 成長ホルモン / 脳内トランスポーター / 誘導体化 / アフィニティーラベル化 / SLC10A4 |
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| Research Abstract |
According to proteomics approach, we tried to develop an analytical method for bile acid signal transduction and clarify the function of bile acids in the brain. The results by the affinity labeling analysis of growth hormone using chenodeoxycholyl adenylate showed that chenodeoxycholate binds to the region different from the growth hormone receptor binding site. Next, we developed an analytical method of phosphorylated proteins using the specific derivatization and the double pseudo-neutral loss extraction on nanoLC/ESI-MS/MS. We found its usefulness for identification of phosphorylated proteins in complex protein mixtures and identification of phosphorylated sites. In addition, we extracted chenodeoxycholate-binding proteins in hepatocytes by using the chenodeoxycholate-immobilized cleavable affinity gel, and identified dihydrodiol dehydrogenase, which is known as a bile acid-binding protein in the hepatocyte, and peroxiredoxin 1.
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