Study on the functions of WRN and WRNIP1 that interacts with WRN
| Project/Area Number |
20390020
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Tohoku University |
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Principal Investigator |
ENOMOTO Takemi Tohoku University, 薬学研究所, 教授 (80107383)
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| Co-Investigator(Kenkyū-buntansha) |
TADA Shusuke 帝京平成大学, 薬学部, 教授 (00216970)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2010: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2008: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Keywords | ウェルナー症候群 / 早期老化 / WRN / WRNIP1 / RAD18 / DNA損傷回避 / 複製フォーク / ユビキチン化 / WPN / RAK18 / WRNTP1 |
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| Research Abstract |
In this project, we tried to identify the DNA damage avoidance pathway in which WRNIP1 is involved and to clarify how to affect RAD18 and WRN this pathway, and obtained the following results.
(1) WRNIP1 localized to the region containing DNA single-strand breaks via its N-terminal UBZ domain.
(2) RAD18-RAD6 complex bound to replication fork like DNA recruited WRNIP1 to the DNA, and recruited WRNIP1 replaced RAD18-RAD6 complex.
(3) WRNIP1 bound to template-primer DNA recruited WRN to the DNA, and recruited WRN replaced WRNIP1.
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Report
(4 results)
Research Products
(63 results)
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[Journal Article] Biphasic chromatin binding of FACT during DNA replication.2011
Author(s)
Kundu L.R., Seki M., Watanabe N., Murofusi H, Furukohri A., Waga S., Score A.J., Blow J.J., Horikoshi M., Enomoto T., Tada S.
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Journal Title
Biochim.Biophys.Acta 1813
Pages: 1129-1136
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[Journal Article] Generation and characterization of cells that can be conditionally depleted of mitochondrial SOD2.2009
Author(s)
Takada S., Inoue E., Tano K., Yoshii H., Abe T., Yoshimura A., Akita M., Tada S., Watanabe M., Seki M., Enomoto T.
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Journal Title
Biochem.Biophys.Res.Commun. 379
Pages: 233-238
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[Journal Article] RMI, a new OB-fold complex essential for Bloom syndrome protein to maintain genome stability.2008
Author(s)
Xu D., Guo R., Sobeck A., Bachrati C.Z., Yang J., Enomoto T., Brown G.W., Hoatlin M.E., Hickson I.D., Wang W.
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Journal Title
Gene Dev. 22
Pages: 2843-2855
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[Journal Article] KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation.2008
Author(s)
Abe T., Ishiai M., Hosono Y., Yoshimura A., Tsuji H., Tada S., Adachi N., Koyama H., Takata M., Takeda S., Enomoto T., Seki M.
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Journal Title
Cell Signal. 20
Pages: 1978-1985
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[Journal Article] Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIa.2008
Author(s)
Otsuki M., Seki M., Inoue E., Abe T., Narita Y., Yoshimura A., Tada S., Ishii Y., Enomoto T.
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Journal Title
Biochim.Biophys.Acta 1782
Pages: 75-81
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