Pathogenetic mechanism of sporadic Parkinson's disease based on familial Parkinson's disease
| Project/Area Number |
20390040
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | Hiroshima University |
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Principal Investigator |
OHTA Shigeru Hiroshima University, 大学院・医歯薬学総合研究科, 教授 (60160503)
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| Co-Investigator(Kenkyū-buntansha) |
KOTAKE Yaichiro 広島大学, 大学院・医歯薬学総合研究科, 准教授 (20335649)
SUGIHARA Kazumi 広島国際大学, 薬学部, 准教授 (20271067)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2008: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | パーキンソン病 / β-チューブリン / ユビキチン化 / チューブリン / parkin / ユビキチン化阻害 / テトラハイドロイソキノリン / Parkin |
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| Research Abstract |
We have reported that 1BnTIQ, which is increased in parkinsonian cerebrospinal fluid, binds to β-tubulin and inhibits its ubiquitination. In this study, we examined the effect of MPP^+ on β-tubulin ubiquitination. MPP^+ (10μM) reduced the content ubiquitinated β-tubulin. Consistent with this, β-tubulin in 1% Nonidet P-40-insoluble fraction was accumulated in a time-dependent manner. Thus, the inhibition of tubulin ubiquitination and its accumulation may involved in neuronal cell death in Parkinson's disease.
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Report
(4 results)
Research Products
(56 results)
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[Journal Article] Crystal structure, docking study and structure-activity relationship of carborane-containing androgen receptor antagonist 3- (12-hydroxymethy1-1, 12-dicarba-closo-dodecaboran-l-yObenzonitrile.2011
Author(s)
Ohta K, Goto T, Fujii S, Kawahata M, Oda A, Ohta S, Yamaguchi K, Hirono S, Endo Y.
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Journal Title
Bioorg.Med.Chem. 19
Pages: 3540-3548
Related Report
Peer Reviewed
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[Journal Article] 1-Benzyl-1,2,3,4-tetrahydroisoquinoline binds with tubulin beta, a substrate of parkin, and reduces its polyubiquitination.2010
Author(s)
Kohta R, Kotake Y, Hosoya T, Hiramatsu T, Otsubo Y, Koyama H, Hirokane Y, Yokoyama Y, Ikeshoji H, Oofusa K, Suzuki M, Ohta S.
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Journal Title
J.Neurochem. 114
Pages: 1291-1301
Related Report
Peer Reviewed
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[Journal Article] Approach for in vivo protein binding of 5-n-butyl-yrazolo [1,5-a] pyrimidine bioactivated in chimeric mice with humanized liver by two-dimensional electrophoresis with accelerator mass spectrometry.2010
Author(s)
Yamazaki H, Kuribayashi S, Inoue T, Tateno C, Nishikura Y, Oofusa K, Harada D, Naito S, Horie T, Ohta S.
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Journal Title
Chem.Res Toxicol. 23
Pages: 152-158
Related Report
Peer Reviewed
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