Functional morphologic study of the peripheral cholinergic nervous system in human.

• Project/Area Number: 20390051
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Shiga University of Medical Science
• Principal Investigator: KIMURA Hiroshi Shiga University of Medical Science, 名誉教授 (40079736)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2008: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: 末梢型コリンアセチル基転移酵素 / アセチルコリン / 腸管神経系 / アミノ酸配列 / 抗体作成 / 免疫組織化学 / 神経伝達物質 / ヒト腸管神経系 / コリンアセチル基転移酵素 / pChAT / 酵素精製 / モノクローナル抗体

Research Abstract

We have previously demonstrated that the neurotransmitter acetylcholine is synthesized by two enzymes, cChAT and pChAT. The pChAT occurs not only in peripheral cholinergic neurons but also in neuronal crest derived neurons (primary sensory, postganglionic sympathetic and intramural enteric neurons) of experimental animals. The present study aims at examining whether pChAT is also present in humans. Using protein purification and antibody production methods, we have firstly succeeded to visualize neurons containing human pChAT.

Research Products

• [Journal Article] Peripheral type of choline acetyltransferase : Biological and evolutionary implications for novel mechanisms in cholinergic system (2011)
  • Author(s): Bellier JP, Kimura H
  • Journal Title: J Chem Neuroanat.
  • Peer Reviewed
• [Journal Article] Immunohistochemical demonstration of cholinergic structures in central ganglia of the slug (Limax maximus, Limax valentianus). (2011)
  • Author(s): D'Este L, Casini A, Kimura S, Bellier JP, Ito E, Kimura H, Renda TG.
  • Journal Title: Neurochem Int. Vol 58(5) Pages: 605-611
  • Peer Reviewed
• [Journal Article] Choline acetyltransferase of the common type immunoreactivity in the rat brain following different heroin treatments : a pilot study (2011)
  • Author(s): Casini A, Vivacqua G, Pontieri FE, Kimura H, Bellier JP, D'Este L, Renda TG
  • Journal Title: J Chem Neuroanat. Vol.41(2) Pages: 111-121
  • Peer Reviewed
• [Journal Article] Nuclear choline acetyltransferase activates transcription of a high-affinity choline transporter (2011)
  • Author(s): Matsuo A, Bellier JP, Nishimura M, Yasuhara O, Saito N, Kimura H
  • Journal Title: J Biol Chem Vol.286(7) Pages: 5836-5845
  • Peer Reviewed
• [Journal Article] Peripheral type of choline acetyltransferase : Biological and evolutionary implications for novel mechanisms in cholinergic system.(2011). (2011)
  • Author(s): Bellier JP, Kimura H.
  • Journal Title: J Chem Neuroanat. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Immunohistochemical demonstration of cholinergic structures in central ganglia of the slug (Limax maximus, Limax valentianus). (2011)
  • Author(s): D'Este L, Casini A, Kimura S, Bellier JP, Ito E, Kimura H, Renda TG.
  • Journal Title: Neurochem Int. Volume: 58(5) Pages: 605-611
  • Peer Reviewed
• [Journal Article] Choline acetyltransferase of the common type immunoreactivity in the rat brain following different heroin treatments : a pilot study. (2011)
  • Author(s): Casini A, Vivacqua G, Pontieri FE, Kimura H, Bellier JP, D'Este L, Renda TG.
  • Journal Title: J Chem Neuroanat. Volume: 41(2) Pages: 111-121
  • Peer Reviewed
• [Journal Article] Nuclear choline acetyltransferase activates transcription of a high-affinity choline transporter. (2011)
  • Author(s): Matsuo A, Bellier JP, Nishimura M, Yasuhara O, Saito N, Kimura H.
  • Journal Title: J Biol Chem. Volume: 286(7) Pages: 5836-5845
  • Peer Reviewed
• [Journal Article] Presynaptic inhibition of primary afferents by depolarization : Observations supporting non-traditional mechanisms (2010)
  • Author(s): Hochman S, Shreckengost J, Kimura H, Quevedo J.
  • Journal Title: Annals of the New York Academy of Science (In press)
  • Peer Reviewed
• [Journal Article] Axotomy alters alternative splicing of choline acetyltransferase in the rat dorsal motor nucleus of the vagus nerve (2009)
  • Author(s): Saito A, Sato T, Okano H, Toyoda K, Bamba H, Kimura S, Bellier J-P, Matsuo A, Hisa Y, Tooyama I.
  • Journal Title: Journal of Comparative Neurololgy 513 Pages: 237-48
  • Peer Reviewed
• [Journal Article] Axotomy alters alternative splicing of choline acetyltransferase in the rat dorsal motor nucleus of the vagus nerve. (2009)
  • Author(s): Saito A
  • Journal Title: Journal of Comparative Neurology 513 Pages: 237-248
  • Peer Reviewed
• [Journal Article] First visualization of cholinergic cells and fibers by immunohistochemistry for choline acetyltransferase of the common type in the optic lobe and peduncle complex of octopus vulgaris. (2008)
  • Author(s): D' Este L
  • Journal Title: Journal of Comparative Neurology 509(6) Pages: 566-579
  • Peer Reviewed
• [Journal Article] Distribution of a splice variant of choline acetyltrans ferase in the trigeminal ganglion and brainstem of the rat : comparison with calcitonin gene-related peptide and substance P. (2008)
  • Author(s): Yasuhara O
  • Journal Title: Journal of Comparative Neurology 509(4) Pages: 436-448
  • Peer Reviewed
• [Presentation] コリン神経研究の最近の進歩 : 末梢型アセチルコリン合成酵素の同定 (2009)
  • Author(s): 木村宏
  • Organizer: 第50回日本組織細胞化学会総会
  • Place of Presentation: 滋賀県大津市
  • Year and Date: 2009-09-26
• [Presentation] Recent advances in cholinergic mechanisms with special reference to the identification of choline acetyltransferase of the peripheral type (pChAT) (2009)
  • Author(s): 木村宏
  • Organizer: 第33回イタリア組織化学会 (シンポジウム)
  • Place of Presentation: 滋賀県大津市
  • Year and Date: 2009-06-09