Clarification of molecular mechanisms of abnormal vascular contraction by functional proteomics and a single molecular analysis of signal transduction

• Project/Area Number: 20390059
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Yamaguchi University
• Principal Investigator: KOBAYASHI Sei Yamaguchi University, 大学院・医学系研究科, 教授 (80225515)
• Co-Investigator(Kenkyū-buntansha): KISHI Hiroko 山口大学, 大学院・医学系研究科, 講師 (40359899) ; KAJIYA Katsuko 山口大学, 大学院・医学系研究科, 助教 (00379942) ; KAWAMICHI Hozumo 山口大学, 大学院・医学系研究科, 助教 (80363042)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000); Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2008: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
• Keywords: 病態生理 / 血管病 / 1分子 / シグナル伝達 / 機能的プロテオミクス / 質量分析

Research Abstract

We tried to clarify the molecular mechanisms of abnormal vascular contraction by functional proteomics and a single molecular analysis of signal transduction. We identified some candidates for novel signaling molecules for abnormal vascular contraction. We found that myosin light chain are phosphorylated by Rho-kinase in the absence of Ca2+ and can rapid sliding with actin. We observed the interaction between SPC and lipid raft model membrane.

Research Products

• [Journal Article] Focused proteomicsによる、血管平滑筋異常収縮の新規病的シグナル分子の探索 (2010)
  • Author(s): 岸博子, ら
  • Journal Title: 日本平滑筋学会雑誌 14巻
  • Peer Reviewed
• [Journal Article] Focused proteomic approach to identify novel signaling molecules for abnormal vascular smooth muscle contraction. (2010)
  • Author(s): 岸博子, et al.
  • Journal Title: J Physiol Sci 60 (Supp)
  • Peer Reviewed
• [Journal Article] Focused Proteomicsによる、血管平滑筋異常収縮の新規病的シグナル分子の探索 (2010)
  • Author(s): 岸博子, ら
  • Journal Title: 日本平滑筋学会雑誌 Volume: 14
  • Peer Reviewed
• [Journal Article] Focused proteomic approach to identify novel signaling molecules for abnormal vascular smooth muscle contraction (2010)
  • Author(s): Hiroko Kishi, et al.
  • Journal Title: The Journa of Physiological Sciences Volume: 60(Supplement 1)
  • Peer Reviewed
• [Journal Article] Elevated concentrations of sphingosylphosphorylcholine in cerebrospinal fluid after subarachnoid hemorrhage : A possible role as a spasmogen. (2009)
  • Author(s): T.Kurokawa, Y.Yumiya, H.Fujisawa, S.Shirao, S.Kashiwagi, M.Sato, H.Kishi, S.Miwa, K.Mogami, S.Kato, T.Akimura, M.Soma, K.Ogasawara, A.Ogawa, S.Kobayashi, M.Suzuki
  • Journal Title: J Clin Nuerosci 16 Pages: 1064-1068
  • Peer Reviewed
• [Journal Article] 血管異常収縮の分子機構と分子標的治療薬の探索 (2009)
  • Author(s): 川道穂津美, ら
  • Journal Title: 日薬理誌 133巻 Pages: 124-129
  • NAID: 10025740121
• [Journal Article] Elevated concentrations of sphingosylphosphorylcholine in cerebrospinal fluid after subarachnoid hemorrhage : A possible role as a spasmogen (2009)
  • Author(s): Kurokawa T, et al.
  • Journal Title: J.Clin. Nuerosci. 16 Pages: 1064-1068
  • Peer Reviewed
• [Journal Article] 血管異常収縮の分子機構と分子標的治療薬の探索 (2009)
  • Author(s): 川道穂津美ら
  • Journal Title: 日薬理誌 133 Pages: 124-129
  • NAID: 10025740121
• [Journal Article] スフィンゴ脂質による血管収縮のカルシウム感受性制御の血管病における意義 (2008)
  • Author(s): 加治屋勝子, ら
  • Journal Title: 血管医学 9巻 Pages: 231-238
• [Journal Article] スフィンゴ脂質による血管収縮のカルシウム感受性制御の血管病における意義 (2008)
  • Author(s): 加治屋勝子ら
  • Journal Title: 血管医学 9 Pages: 231-238
• [Presentation] 血管平滑筋収縮のCa2+-sensitizationを特異的に阻害する植物由来成分 (2010)
  • Author(s): 宮成健司, ら
  • Organizer: 筋生理の集い
  • Place of Presentation: 東京(東京慈恵会医科大学)
  • Year and Date: 2010-12-04
• [Presentation] 血管平滑筋収縮のCa^<2+>-sensitizationを特異的に阻害する植物由来成分 (2010)
  • Author(s): 宮成健司, ら
  • Organizer: 筋生理の集い
  • Place of Presentation: 東京(東京慈恵会医科大学)
  • Year and Date: 2010-12-04
• [Presentation] Essential roles of Fyn tyrosine kinase and membrane lipid raft in smooth muscle contraction (2009)
  • Author(s): 小林誠
  • Organizer: International Symposium Post-genomic advances in the physiology of smooth muscle
  • Place of Presentation: 名古屋市立大学
  • Year and Date: 2009-07-23
• [Presentation] Essential roles of Fyn tyrosine kinase and membrane lipid raft in Ca2+-sensitization of vascular smooth mudcle contraction. (2009)
  • Author(s): Kobayashi S
  • Organizer: International Symposium Post-genomic Advances in the Physiology of Smooth Muscle
  • Place of Presentation: 名古屋市立大学
  • Year and Date: 2009-07-23
• [Presentation] 血管平滑筋収縮のCa感受性増強におけるFynチロシンキナーゼの重要性 (2008)
  • Author(s): 小林誠
  • Organizer: 適応医学会
  • Place of Presentation: 別府ビーコンプラザ
  • Year and Date: 2008-06-06
• [Presentation] 血管平滑筋収縮のCa^<2+>感受性増強におけるFynチロシンキナーゼ. の重要性 (2008)
  • Author(s): 小林 誠
  • Organizer: 適応医学会第12回学術集会
  • Place of Presentation: ビーコンプラザ、別府
  • Year and Date: 2008-06-06
• [Remarks] ホームページ等
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• [Remarks]
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• [Remarks]
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