Regulation of intracellular localization of ENaC by protease

• Project/Area Number: 20390060
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: MARUNAKA Yoshinori 京都府立医科大学, 大学院・医学研究科, 教授 (00127036)
• Co-Investigator(Kenkyū-buntansha): NIISATO Naomi 京都府立医科大学, 医学研究科, 准教授 (00237645) ; NAKAJIMA Ken-ichi 京都府立医科大学, 医学研究科, 助教 (40398392) ; KUSUZAKI Katsuyuki 平安女学院, 文化創造センター, 客員教授 (30177993) ; ASHIHARA Eishi 京都府立医科大学, 医学研究科, 講師 (70275197) ; HOSOGI Shigekuni 京都府立医科大学, 医学研究科, 助教 (30405261) ; 宮崎 裕明 京都府立医科大学, 医学研究科, 助教 (30360027) ; 山田 敏樹 京都府立医科大学, 医学部, プロジェクト研究員 (10453101)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2009: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2008: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: ENaC / アルデステン / 皮質集合管 / プロテアーゼ阻害剤 / 浸透圧 / トラッフィッキング / 高血圧 / ナトリウム輸送 / 透浸圧 / アルドステロン / 腎皮質集合管

Research Abstract

We tried to clarify the regulation of intracellular localization of ENaC by protease. We have found that : 1) aldosterone increases expression of protease, and 2) aldosterone elevates the recycling efficiency of ENaC in the intracellular space by cleavage of ENaC mediated through protease.

Research Products

• [Journal Article] Hypotonic stress upregulates beta-and gamma-ENaC expression through suppression of ERK by inducing MKP-1 (2012)
  • Author(s): Niisato N, Ohta M, Eaton DC, Marunaka Y
  • Journal Title: Am J Physiol Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Sensing mechanism of stretch activated ion channels Mechanically Gated Channels and mechanisms of their regulation/. Mechanosensitivity in Cells and Tissues. (2012)
  • Author(s): Niisato N, Marunaka Y.
  • Journal Title: Springer Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Procaterol-stimulated increases in ciliary bend amplitude and ciliary beat frequency in mouse bronchioles. (2012)
  • Author(s): Komatani-Tamiya N, Daikoku E, Takemura Y, Shimamoto C, Nakano T, Iwasaki Y, Matsumura H, Marunaka Y, Nakahari T
  • Journal Title: Cell Physiol Biochem Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Blockade of chloride ion transport enhances the cytocidal effect of hypotonic solution in gastric cancer cells (2012)
  • Author(s): Iitaka D, Shiozaki A, Ichikawa D, Kosuga T, Komatsu S, Okamaoto K, Fujiwara H, Ishii H, Nakahari T, Marunaka Y, Otsuji E
  • Journal Title: J Surgical Res Volume: 176(2) Pages: 524-534
  • Peer Reviewed
• [Journal Article] Hypotonic stress upregulates beta- and gamma-ENaC expression through suppression of ERK by inducing MKP-1 (2012)
  • Author(s): Niisato N, Ohta M, Eaton DC, Marunaka Y
  • Journal Title: Am J Physiol (Renal Physiol) Volume: 303(2) Pages: 240-252
  • Peer Reviewed
• [Journal Article] Regulation of epithelial sodium transport via epithelial Na^+channel(ENaC) (2011)
  • Author(s): MARUNAKA Y, Niisato N, Taruno A, Ohta M, Miyazaki H, Hosogi S, Nakajima K, Kusuzaki K, Ashihara E, Nishio K, Iwasaki Y, Nakahari T, Kubota T
  • Journal Title: J Biomed Biotechnol Volume: 2011 Pages: 978196-978196
  • Peer Reviewed
• [Journal Article] Quercetin stimulates NGF-induced neurite outgrowth in PC12 cells via activation of Na^+/K^+/2Cl^-cotransporter (2011)
  • Author(s): Nakajima K, Niisato N, MARUNAKA Y
  • Journal Title: Cell Physiol Biochem Volume: 28 Pages: 147-156
  • Peer Reviewed
• [Journal Article] Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma (2011)
  • Author(s): Kosuga T, Shiozaki A, Ichikawa D, Fujiwara H, Kotnatsu S, Iitaka D, Tsujiura M, Morimura R, Takeshita H, Nagata H, Okamoto K, Nakahari T, Marunaka Y, Otsuji E
  • Journal Title: Oncolgy Reports Volume: 26 Pages: 577-586
  • Peer Reviewed
• [Journal Article] Quercetin stimulates NGF-induced neurite outgrowth in PC12 cells via activation of Na+/K+/2Cl-cotransporter (2011)
  • Author(s): Nakajima K, Niisato N, Marunaka Y
  • Journal Title: Cell Physiol Biochem Volume: 28 Pages: 351-356
  • Peer Reviewed
• [Journal Article] Genistein enhances the NGF-induced neurite outgrowth (2011)
  • Author(s): Nakajima K, Niisato N, Marunaka Y
  • Journal Title: Biomed Res Volume: 32(5) Pages: 351-356
  • NAID: 130004724062
  • Peer Reviewed
• [Journal Article] Regulation of epithelial sodium transport via epithelial Na^+ channel (ENaC) (2011)
  • Author(s): Marunaka Y, Niisato N, Taruno A, Ohta M, Miyazaki H, Hosogi S, Nakajima K, Kusuzaki K, Ashihara E, Nishio K, Iwasaki Y, Nakahari T, Kubota T
  • Journal Title: J Biomed Biotechnol Volume: 2011 Issue: 1 Pages: 978196-978196
  • DOI: 10.1155/2011/978196
  • Peer Reviewed
• [Journal Article] KF-1 Ubiquitin Ligase : Anxiety Suppressor Model. (2011)
  • Author(s): Hashimoto-Gotoh T, Iwabe N, Tsujimura A, Nakagawa M, Marunaka Y.
  • Journal Title: Cell Biochem Biophys Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Analysis of blocker-labeled channels reveals the dependence of recycling rates of ENaC on the total amount of recycled channels (2010)
  • Author(s): Taruno A, Marunaka Y
  • Journal Title: Cell Phsyiol Biochem Volume: 26巻 Pages: 925-934
  • Peer Reviewed
• [Journal Article] PPAR-alpha modulation of Ca^<2+>-regulated exocytosis via arachidonic acid in guinea pig antral mucous cells (2010)
  • Author(s): Sawabe Y, Shimamoto C, Sakai A, Kuwabara H, Saad AH, Nakano T, Takitani K, Tamai H, Mori H, MARUNAKA Y, Nakahari T
  • Journal Title: Exp Physiol Volume: 95 Pages: 858-868
  • Peer Reviewed
• [Journal Article] NaCl flux between apical and basolateral side recruits claudin-1 to tight junction strands and regulate paracellular transport (2010)
  • Author(s): Tokuda S, Miyazaki H, Nakajima K, Yamada T, MARUNAKA Y
  • Journal Title: Biochem Biophys Res Commun Volume: 393 Pages: 390-396
  • Peer Reviewed
• [Journal Article] Analysis of blocker-labeled channels reveals the dependence of recycling rates of ENaC on the total amount of recycled channels. (2010)
  • Author(s): Taruno A, Marunaka Y.
  • Journal Title: Cell Phsyiol Biochem 26 Pages: 925-934
• [Journal Article] NaCl flux between apical and basolateral side recruits claudin-1 to tight junction strands and regulate paracellular transport. (2010)
  • Author(s): Tokuda S, Miyazaki H, Nakajima K, Yamada T, Marunaka Y.
  • Journal Title: Biochem Biophys Res Commun 393 Pages: 390-396
• [Journal Article] Photodynamic therapy with acridine orange in musculoskeletal sarcomas. (2010)
  • Author(s): Matsubara T, Kusuzaki K, Matsumine A, Murata H, Marunaka Y, Hosogi S, Uchida A, Sudo A.
  • Journal Title: J Bone Joint Surg Br. Volume: 92(6) Pages: 760-762
  • Peer Reviewed
• [Journal Article] Peroxisome proliferation activation receptor alpha modulation of Ca^<2+>-regulatedexocytosis via arachidonic acid in guinea-pig antral mucous cells. (2010)
  • Author(s): Sawabe Y, Shimamoto C, Sakai A, Kuwabara H, Saad AH, Nakano T, Takitani K, Tamai H, Mori H, Marunaka Y, Nakabari T
  • Journal Title: Exp Physiol. Volume: 95(8) Pages: 858-868
  • Peer Reviewed
• [Journal Article] Photodynamic therapy with acridine orangein musculoskeletal sarcomas. (2010)
  • Author(s): Matsubara T, Kusuzaki K, Matsumine A, Murata H, Marunaka Y, Hosogi S, Uchida A, Sudo A.
  • Journal Title: J Bone Joint Surg (Br). Volume: 92(6) Pages: 760-762
  • Peer Reviewed
• [Journal Article] Analysis of blocker-labeled channels reveals the dependence of recycling rates of ENaC on the total amount of recycled channels (2010)
  • Author(s): Taruno A, Marunaka Y.
  • Journal Title: Cell Phsyiol Biochem. Volume: 26(6) Pages: 925-934
  • Peer Reviewed
• [Journal Article] Chloride ion modulates cell proliferation of human androgen-independent prostatic cancer cell. (2010)
  • Author(s): Hiraoka K, Miyazaki H, Niisato N, Iwasaki Y, Kawauchi A, Miki T, Marunaka Y.
  • Journal Title: Cell Physiol Biochem. 25(4-5) Pages: 379-388
  • Peer Reviewed
• [Journal Article] Intracellular chloride regulates cell proliferation through the activation of stress-activated protein kinases in MKN28 human gastric cancer cells. (2010)
  • Author(s): Ohsawa R, Miyazaki H, Niisato N, Shiozaki A, Iwasaki Y, Otsuji E, Marunaka Y.
  • Journal Title: J Cell Physiol. 223(3) Pages: 764-770
  • Peer Reviewed
• [Journal Article] NaCl flux between apical and basolateral side recruits claudin-1 to tight junction strands and regulates paracellular transport. (2010)
  • Author(s): Tokuda S, Miyazaki H, Nakajima K, Yamada T, Marunaka Y.
  • Journal Title: Biochem Biophys Res Commun. 393(3) Pages: 390-396
  • Peer Reviewed
• [Journal Article] Hydrostatic pressure regulates tight junctions, actin cytoskeleton and transcellular on transport (2009)
  • Author(s): Tokuda S, Miyazaki H, Nakajima K, Yamada T, MARUNAKA Y
  • Journal Title: Biochem Biophys Res Commun Volume: 390 Pages: 1315-1321
  • Peer Reviewed
• [Journal Article] Regulation of paracellular Na^+and Cl^-conductances by hydrostatic pressure (2009)
  • Author(s): Tokuda S, Niisato N, Nagai T, Taruno A, Nakajima K, Miyazaki H, Yamada T, Hosogi S, Ohta M, Nishio K, Iwasaki Y, MARUNAKA Y
  • Journal Title: Cell Biol Int Volume: 33 Pages: 949-956
  • Peer Reviewed
• [Journal Article] Effects of extracellular chloride ion on epithelial sodium channel(ENaC) in arginine vasotocin(AVT)-stimulated renal epithelial cells (2009)
  • Author(s): Yamada T, Niisato N, MARUNAKA Y
  • Journal Title: Biomed Res Volume: 30 Pages: 193-198
  • NAID: 130004470752
  • Peer Reviewed
• [Journal Article] Hydrostatic pressure regulates tight junctions, actin cytoskeleton and transcellular ion transport. (2009)
  • Author(s): Tokuda S, Miyazaki H, Nakajima K, Yamada T, Marunaka Y.
  • Journal Title: Biochem Biophys Res Commun 390 Pages: 1315-1321
• [Journal Article] Regulation of paracellular Na+ and Cl- conductances by hydrostatic pressure. (2009)
  • Author(s): Tokuda S, Niisato N, Nagai T, Taruno A, Nakajima K, Miyazaki H, Yamada T, Hosogi S, Ohta M, Nishio K, Iwasaki Y, MARUNAKA Y.
  • Journal Title: Cell Biol Int 33 Pages: 949-956
• [Journal Article] Hydrostatic pressure regulates tight junctions, actin cytoskeleton and transcellular iontransport. (2009)
  • Author(s): Tokuda S, Miyazaki H, Nakajima K, Yamada T, Marunaka Y.
  • Journal Title: Biochem Biophys Res Commun. 390(4) Pages: 1315-1321
  • Peer Reviewed
• [Journal Article] Two cases of complex sleep apnea syndrome. (2009)
  • Author(s): Yamada T, Arimoto T, Koyama Y, Masui A, Tamiya N, Hosogi S, Hiramatu A, Nakanishi M, Kohno Y, Ueda M, Iwasaki Y, Marunaka Y.
  • Journal Title: Nihon Kokyuki Gakkai Zasshi 47(9) Pages: 793-797
  • NAID: 10026272278
  • Peer Reviewed
• [Journal Article] Phase II trial of weekly gemcitabine and split-dose cisplatin for advanced non-small-cell lung cancer. (2009)
  • Author(s): Hiramatsu A, Iwasaki Y, Koyama Y, Tamiya N, Hosogi S, Nakanishi M, Kohno Y, Ueda M, Arimoto T, Marunaka Y.
  • Journal Title: Jpn J Clin Oncol 39(12) Pages: 779-783
  • Peer Reviewed
• [Journal Article] Effects of extracellular chloride ion on epithelial sodium channel (ENaC) in arginine vasotocin (AVT)-stimulated renal epithelial cells. (2009)
  • Author(s): Yamada T, Niisato N, Marunaka Y.
  • Journal Title: Biomed Res. 30(3) Pages: 193-198
  • NAID: 130004470752
  • Peer Reviewed
• [Journal Article] Regulation of paracellular Na+ and Cl(-) conductances by hydrostatic pressure (2009)
  • Author(s): Tokuda S, Niisato N, Nagai T, Taruno A, Nakajima K, Miyazaki H, Yamada T, Hosogi S, Ohta M, Nishio K, Iwasaki Y, Marunaka Y.
  • Journal Title: Cell Biol Int. 33(9) Pages: 949-956
  • Peer Reviewed
• [Journal Article] Blockers of K+/Cl- transporter/channels diminish proliferation of osteoblastic cells. (2009)
  • Author(s): Maki M, Miyazaki H, Niisato N, Morihara T, Marunaka Y, Kubo T.
  • Journal Title: Biomed Res. 30(2) Pages: 137-140
  • NAID: 130004470739
  • Peer Reviewed
• [Journal Article] Action of N-acylated ambroxol derivatives on secretion of chloride ions in human airway epithelia. (2009)
  • Author(s): Yamada T, Takemura Y, Niisato N, Mitsuyama E, Iwasaki Y, Marunaka Y.
  • Journal Title: Biochem Biophys Res Commun. 380(3) Pages: 586-590
  • Peer Reviewed
• [Journal Article] Quercetin stimulates Na+/K+/2Cl-cotransport via PTK-dependent mechanisms in human airway epithelium. (2009)
  • Author(s): Asano J, Niisato N, Nakajima K, Miyazaki H, Yasuda M, Iwasaki Y, Hama T, Dejima K, Hisa Y, Marunaka Y.
  • Journal Title: Am J Respir Cell Mol Biol. 41(6) Pages: 688-695
  • Peer Reviewed
• [Journal Article] Atypical antipsychotics suppress production of proinflammatory cytokines and up-regulate interleukin-10 in lipopolysaccharide-treated mice. (2009)
  • Author(s): Sugino H, Futamura T, Mitsumoto Y, Maeda K., Marunaka Y.
  • Journal Title: Prog Neuropsychopharmacol Biol Psychiatry. 33(2) Pages: 303-307
  • Peer Reviewed
• [Journal Article] Action of N-acylated ambroxol derivatives on secretion of chloride ions in human airway epithelia (2009)
  • Author(s): Yamada T, Takemura Y, Niisato N, Mitsuyama E, Iwasaki Y, MARUNAKA Y.
  • Journal Title: Biochem. Biophys. Res. Commun. 380 Pages: 586-590
  • Peer Reviewed
• [Journal Article] Quercetin stimulates NKCC1 via a PTK-dependent mechanism in human airway epithelium (2009)
  • Author(s): Maki M, Miyazaki H, Niisato N, Morihara M, MARUNAKA Y, Kubo T
  • Journal Title: Biomed. Res. 30 Pages: 137-140
  • Peer Reviewed
• [Journal Article] Regulation of the paracellular Na^+and Cl^-conductances by the NaCl-generated osmotic gradient in a manner dependent on the direction of osmotic gradients (2008)
  • Author(s): Tokuda S, Niisato N, Nakajima K, MARUNAKA Y
  • Journal Title: Biochem Biophys Res Commun Volume: 366 Pages: 464-470
  • Peer Reviewed
• [Journal Article] Chloride ions control the G1/S cell-cycle checkpoint by regulating the expression of p21 through a p53-independent pathway in human gastric cancer cells (2008)
  • Author(s): Miyazaki H, Shiozaki A, Niisato N, Ohsawa R, Itoi H, Ueda Y, Otsuji E, Yamagishi H, Iwasaki Y, Nakano T, Nakahari T, MARUNAKA Y
  • Journal Title: Biochem Biophys Res Commun Volume: 366 Pages: 506-512
  • Peer Reviewed
• [Journal Article] Intracellular calcium plays a role as the second messenger of hypotonic stress in gene regulation of SGK1 and ENaC in renal epithelial A6 cells (2008)
  • Author(s): Taruno A, Niisato N, MARUNAKA Y
  • Journal Title: Am J Physiol Renal Physiol Volume: 294
  • Peer Reviewed
• [Journal Article] Osmortransduction through volume-sensitive Cl-channels. Mechanosensitive ion channels/Mechanosensitivity in Cells and Tissues (2008)
  • Author(s): Niisato N, MARUNAKA Y
  • Journal Title: Springer Volume: Vol.1 Pages: 179-202
  • Peer Reviewed
• [Journal Article] Intracellular calcium plays a role as the second messenger of hypotonic stress in gene regulation of SGK1 and ENaC in renal epithelial A6 cells. (2008)
  • Author(s): Taruno A, Niisato N, Marunaka Y.
  • Journal Title: Am J Physiol Renal Physiol 294
• [Journal Article] Effect of inducible nitric oxide synthase on apoptosis in Candia-inducedacute lung injury (2008)
  • Author(s): Hosogi S, Iwasaki, Yamada T, Komatani-Tamiya, Hiramatsu A, Kohno Y, Ueda M, Arimoto T, MARUNAKA Y
  • Journal Title: Biomed. Res. 29 Pages: 257-266
  • Peer Reviewed
• [Journal Article] Effects of extracellular chloride ion on epihtelial sodium channel (ENaC) in arginine vasotocin (AVT)-stimulated renal epihtelial cells
  • Author(s): Yamada T, Niisato N, MARUNAKA Y.
  • Journal Title: Biomed. Res. (印刷中)
  • Peer Reviewed
• [Journal Article] Quercetin stimulates NKCC1 via a PTK-dependent mechanism in human airway epithelium
  • Author(s): Asano J, Niisato N, Nakajima K, Miyazaki H, Yasuda M, Iwasaki Y, Hama T, Dejima K, Hisa Y, MARUNAKA Y.
  • Journal Title: Am. J. Resp. Cell Mol. Biol. (印刷中)
  • Peer Reviewed
• [Presentation] Recycling rates of ENaC depend on the total amount of recycled channels (2012)
  • Author(s): Marunaka Y, Taruno A
  • Organizer: The 89^<th> Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Matsumoto
• [Presentation] p-38-dependent suppression of ENaC endocytosis is involved in stimulation of Na^+ reabsorption by aldosterone in renal epithelial A6 cells (2012)
  • Author(s): Niisato N, Ohta M, Marunaka Y
  • Organizer: The 89^<th> Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Matsumoto
• [Presentation] Analysis of epithelial ion transport by mathematical model (2012)
  • Author(s): Sasamoto Kouhei, Niisato Naomi, Marunaka Yoshinori
  • Organizer: The 89^<th> Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Matsumoto
• [Presentation] The mechanisms of Na^+/H^+ exchanger inhibitor on proliferation of gastric cancer cells with several pathways (2012)
  • Author(s): Hosogi Shigekuni, Ohta Mariko, Nakajima Ken-ichi, Ashihara Eishi, Niisato Naomi, Marunaka Yoshinori
  • Organizer: The 89^<th> Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Matsumoto
• [Presentation] Measurement of intracellular chloride concentration in differentiating PC12 cells (2012)
  • Author(s): Nakajima Ken-ichi, Niisato Naomi, Marunaka Yoshinori
  • Organizer: The 89^<th> Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Matsumoto
• [Presentation] Regulatory Mechanism of Epithelial Na^+ Channel (ENaC) (2011)
  • Author(s): Marunaka, Y
  • Organizer: Institute of Developmental Biology and Cancer Research, University of Nice
  • Place of Presentation: Nice(招待講演)
  • Year and Date: 2011-11-17
• [Presentation] イオン環境依存性癌細胞増殖機構 (2011)
  • Author(s): 丸中良典
  • Organizer: 第1回癌イオノミクス研究会
  • Place of Presentation: 京都
  • Year and Date: 2011-11-05
• [Presentation] Regulatory mechanisms of cancer cell growth by ionic environments. International Symposium on Cancer Research (2011)
  • Author(s): Marunaka, Y
  • Organizer: Italy/Japan Joint Meeting on Cancer Research
  • Place of Presentation: Kyoto(招待講演)
  • Year and Date: 2011-11-04
• [Presentation] 神経突起伸張におけるK+/Cl-共輸送体の役割 (2011)
  • Author(s): 中島謙一、永尾光、新里直美、久育男、丸中良典
  • Organizer: 日本生理学会第104回近畿生理学談話会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-01
• [Presentation] 管腔側膜上ENaC発現制御におけるp38の役割 (2011)
  • Author(s): 太田麻利子、新里直美、丸中良典
  • Organizer: 日本生理学会第104回近畿生理学談話会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-01
• [Presentation] 胃癌細胞株MKN-28細胞株のおけるNa+/H+ exchanger (NHE)阻害剤の細胞内pH・Cl-濃度の与える影響と細胞増殖抑制機構 (2011)
  • Author(s): 細木誠之、太田麻利子、中島謙一、宮崎裕明、芦原英司、楠崎克之、新里直美、丸中良典
  • Organizer: 日本生理学会第104回近畿生理学談話会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-01
• [Presentation] クロライドイオン輸送体活性化による神経突起伸長促進機構の解明 (2011)
  • Author(s): 丸中良典、楠崎克之、新里直美、芦原英司、中島謙一、細木誠之、羽柴光起
  • Organizer: ソルト・サイエンス研究財団第23回助成研究発表会
  • Place of Presentation: 東京
  • Year and Date: 2011-07-19
• [Presentation] 気道上皮Calu-3細胞でのFisetinによるアニオン分泌制御 (2011)
  • Author(s): 新里直美、丸中良典
  • Organizer: 第10回肺サーファクタント分子病態研究会
  • Place of Presentation: 札幌
  • Year and Date: 2011-06-25
• [Presentation] マウス細気管支線毛運動における周波数と振幅の調節 (2011)
  • Author(s): 中張隆司、丸中良典
  • Organizer: 第5回iPUC-II (Integrated Pulmonary Circulation Research-II)
  • Place of Presentation: 東京
  • Year and Date: 2011-06-25
• [Presentation] Hypotonicity-induced upregulation of beta-and gamma-ENaC expression through suppression of ERK by inducing MKP-1 (2011)
  • Author(s): Marunaka Y, Niisato N, Ohta M
  • Organizer: The 7th International Symposium on Aldosterone and the ENaC/Degenerin Family of Ion Channels : Molecular Mechanisms and Pathophysiology
  • Place of Presentation: Pacific Grove, California, USA
• [Presentation] Hypotonicity-induced upregulation of beta- and gamma-ENaC expression through suppression of ERK by inducing MKP-1 (2011)
  • Author(s): Marunaka Y, Niisato N, Ohta M
  • Organizer: The 7th International Symposium on Aldosterone and the ENaC/Degenerin Family of Ion Channels : Molecular Mechanisms and Pathophysiology. American Physiological Society
  • Place of Presentation: Pacific Grove(招待講演)
• [Presentation] Involvement of p38-mecliated endocytosis in aldosterone-stimulated Na+ reabsorption in renal epithelial A6 cells (2011)
  • Author(s): Niisato N, Ohata M, Marunaka Y
  • Organizer: The 7th International Symposium on Aldosterone and the ENaC/Degenerin Family of Ion Channels : Molecular Mechanisms and Pathophysiology. American Physiological Society
  • Place of Presentation: Pacific Grove
• [Presentation] Dependence of ENaC recycling rate on the total amount of recycled channels (2011)
  • Author(s): Taruno A, Marunaka Y
  • Organizer: The 7th International Symposium on Aldosterone and the ENaC/Degenerin Family of Ion Channels : Molecular Mechanisms and Pathophysiology. American Physiological Society
  • Place of Presentation: Pacific Grove
• [Presentation] An inhibitor of Na^+/H^+ exchanger (NHE), ethyl-isopropyl amiloride (EIPA), diminishes proliferation of MKN28 human gastric cancer cells by decreasing the intracellular Cl^- concentration via DIDS-sensitive pathway (2011)
  • Author(s): Hosogi S, Ashihara E, Niisato N, Kusuzaki K, Marunaka Y
  • Organizer: The 2nd Symposium of the International Society for Proton Dynamics in Cancer 2011
  • Place of Presentation: Nice
• [Presentation] Hypotonic stress up-regulates Na+ reabsorption through MKP-1-mediated dephosphorylation in renal epithelial A6 cells (2011)
  • Author(s): Niisato N, Ohta M, Marunaka Y
  • Organizer: The 5th International Congress on Stress Response in Biology and Medicine
  • Place of Presentation: Quebec City
• [Presentation] 大豆ゲニステインによる免疫細胞活性化 (2011)
  • Author(s): 丸中良典、芦原英司、新里直美、太田麻利子
  • Organizer: 第14回研究報告会(平成22年度助成課題)
  • Place of Presentation: 大阪
• [Presentation] アルドルテロンによるp38/Akt依存的なナトリウム再吸収制御 (2011)
  • Author(s): 新里直美、西尾恭介、丸中良典
  • Organizer: 第54回日本腎臓学会学術総会
  • Place of Presentation: 横浜
• [Presentation] アルドステロンはp38依存的なENaCエンドサイトーシスを介してNa^+再吸収を制御する (2011)
  • Author(s): 新里直美、太田麻利子、丸中良典
  • Organizer: 2011年度生理学研究所研究会「上皮細胞の恒常性維持機構におけるイオン・物資輸送の新しい分子生理」
  • Place of Presentation: 岡崎
• [Presentation] プロカテロール刺激時PDE1によるマウス細気管支線毛運動の周波数と振幅の調節 (2011)
  • Author(s): 中張隆司、丸中良典
  • Organizer: 2011年度生理学研究所研究会「上皮細胞の恒常性維持機構におけるイオン・物資輸送の新しい分子生理」
  • Place of Presentation: 岡崎
• [Presentation] 上皮組織における細胞外浸透圧感受機構 (2011)
  • Author(s): 新里直美、丸中良典
  • Organizer: 第88回日本生理学会大会、第116回日本解剖学会総会・全国学術集会
  • Place of Presentation: 横浜.シンポジウム
• [Presentation] 上皮型ナトリウムチャネル(ENaC)細胞内輸送速度のENaC総数への依存性 (2011)
  • Author(s): 丸中良典、樽野陽幸
  • Organizer: 第88回日本生理学会大会、第116回日本解剖学会総会・全国学術集会
  • Place of Presentation: 横浜.シンポジウム
• [Presentation] アルドステロン誘因性管腔側膜上ENaC発現増大におけるp38の役割 (2011)
  • Author(s): 太田麻利子、新里直美、丸中良典
  • Organizer: 第88回日本生理学会大会、第116回日本解剖学会総会・全国学術集会
  • Place of Presentation: 横浜
• [Presentation] β_2刺激によるマウス末梢気道線毛運動の活性化:高速度カメラを用いた観察 (2010)
  • Author(s): 中張隆司、大黒恵理子、丸中良典
  • Organizer: 肺サーファクタント分子病態研究会
  • Place of Presentation: 札幌
  • Year and Date: 2010-07-03
• [Presentation] Chloride ion effect on cancer cells. (2010)
  • Author(s): Marunaka Y.
  • Organizer: Symposium on Advanced Biotechnologies & Instrumentations.
  • Place of Presentation: China
• [Presentation] Na^+/H^+ exchanger isoform 1 (NHE-1)-dependent proliferation of gastric cancer cells. (2010)
  • Author(s): Hosogi S, Kusuzaki K, Niisato N, Marunaka Y.
  • Organizer: The 1^<st> Symposium of the International Society for Proton Dynamics in Cancer.
  • Place of Presentation: Rome
• [Presentation] Translational research of photodynamic therapy with acridine orange which targets acidic vesicles and environment of cancer cells. (2010)
  • Author(s): Kusuzaki K, Hosogi S, Matsubara T, Satonaka H, Nakamura T, Matsumine A, Sudo A, Marunaka Y.
  • Organizer: The 1^<st> Symposium of the International Society for Proton Dynamics in Cancer.
  • Place of Presentation: Rome
• [Presentation] Flavonoids stimulate NGF-induced neurite outgrowth in PC12 cells via activation of Na^+/K^+/2Cl^- cotransporter. (2010)
  • Author(s): Nakajima K, Niisato N, Marunaka Y.
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies.
  • Place of Presentation: Hawaii
• [Presentation] Flavonoids stimulate NKCC-mediated Cl- secretion in human airway epithelial Calu-3 cells. (2010)
  • Author(s): Niisato N, Marunaka Y.
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies.
  • Place of Presentation: Hawaii
• [Presentation] クロライドイオンによる癌細胞増殖制御メカニズム (2010)
  • Author(s): 丸中良典
  • Organizer: 第34回近畿肉腫研究会
  • Place of Presentation: 三重
• [Presentation] Regulatory mechanism of Cl^-secretion by flavonoid in human airway epithelial Calu-3 cells. (2010)
  • Author(s): Niisato N, Marunaka Y.
  • Organizer: The 87^<th> Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Morioka
• [Presentation] Cholinergic regulation of epithelial Na^+ channel (ENaC) in alveolar type 2 cell. (2010)
  • Author(s): Takemura Y, Helms My N, Eaton Douglas C, Marunaka Y.
  • Organizer: The 87^<th> Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Morioka
• [Presentation] Flavonoids stimulate NGF-induced neurite outgrowth in PC 12 cells via activation of Na^+/K^+/2Cl^- cotransporter. (2010)
  • Author(s): Nakajima K, Niisato N, Marunaka Y.
  • Organizer: The 87^<th> Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Morioka
• [Presentation] Na^+/H^+ exchanger isoform 1(NHE-1)-dependent proliferation of gastric cancer cells (2010)
  • Author(s): Hosogi S, Nakajima K, Miyazaki H, Niisato N, Marunaka Y.
  • Organizer: The 87^<th> Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Morioka
• [Presentation] The essential role of p38 in aldosterone action on ENaC-mediated Na^+ transport. (2010)
  • Author(s): Ohta M, Niisato N, Marunaka Y.
  • Organizer: The 87^<th> Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Morioka
• [Presentation] Morphological alterations of cell membrane during apoptosis induced by photodynamic therapy with acridine orange. (2010)
  • Author(s): Kusuzaki K, Hosogi S, Ashihara E, Nakajima K, Niisato N, Marunaka Y.
  • Organizer: Membrane Symposium 2010
  • Place of Presentation: Kyoto
• [Presentation] Regulation of Cell Growth by Chloride Ion via MAPK-dependent Pathways. (2010)
  • Author(s): Marunaka Y.
  • Organizer: BIT's 3rd Annual World Congress of PepCon-2010
  • Place of Presentation: China
• [Presentation] プロテアーゼによるENaC寿命決定メカニズム (2009)
  • Author(s): 丸中良典, 新里直美, 宮崎裕明, 中島謙一, 山田敏樹
  • Organizer: 第21回平成20年度助成研究発表会
  • Place of Presentation: 東京
  • Year and Date: 2009-07-21
• [Presentation] ケルセチンはヒト気道上皮においてPTKが関与する経路を介しNa^+/K^+/2CI cotransportを活性化する (2009)
  • Author(s): 安田誠, 浅野純志, 新里直美, 宮崎裕明, 中島謙一, 岩崎吉伸, 浜雄光, 出島健司, 久育男, 丸中良典
  • Organizer: 第3回iPUC-II (Integrtated Pulonary Circulation Research-II)
  • Place of Presentation: 東京
  • Year and Date: 2009-06-27
• [Presentation] ERK dephosphorylation by MKP-1 stimulates Na+ reabsorption through beta- and gamma-ENaC expression in hypotonic stress. (2009)
  • Author(s): Niisato N, Ohta M, Marunaka Y.
  • Organizer: Satellite Symp Intl Union Physiol Sci-2009, Epithelial Ion Transport in Health and Disease.
  • Place of Presentation: Kyoto, Japan.
• [Presentation] Dephosphorylation of ERK by MKP-1 stimulates beta-and gamma-ENaC mRNA expression of renal A6 cells in hypotonic stress. (2009)
  • Author(s): Niisato N, Ohta M, Marunaka Y.
  • Organizer: 40th NIPS International Symposium- International Joint Symposium : PAT-CVR 2009.
  • Place of Presentation: Okazaki, Japan.
• [Presentation] ERK dephosphorylation by MKP-1 stimulates Na^+ reabsorption through beta-and gamma-ENaC expression in hypotonic stress. (2009)
  • Author(s): Niisato N, Ohta M, Marunaka Y.
  • Organizer: Satellite Symposium of International Union of Physiological Sciences-2009, Epithelial Ion Transport in Health and Disease.
  • Place of Presentation: Kyoto
• [Presentation] Intracellular chloride regulates the G1/S cell cycle checkpoint in human gastric cancer MKN28 cells. (2009)
  • Author(s): Miyazaki H, Ohsawa R, Niisato N, Marunaka Y.
  • Organizer: Satellite Symposium of International Union of Physiological Sciences-2009, Epithelial Ion Transport in Health and Disease.
  • Place of Presentation: Kyoto
• [Presentation] The role of chlorideion in dynamics of microtubule. (2009)
  • Author(s): Nakajima K, Niisato N, Marunaka Y.
  • Organizer: Satellite Symposium of International Union of Physiological Sciences-2009, Epithelial Ion Transport in Health and Disease.
  • Place of Presentation: Kyoto
• [Presentation] p38 MAPK regulate ENaC-mediated Na^+ transporty aldosterone in renal epithelial A6 cells. (2009)
  • Author(s): Ohta M, Niisato N, Marunaka Y.
  • Organizer: Satellite Symposium of International Union of Physiological Sciences-2009, Epithelial Ion Transport in Health and Disease.
  • Place of Presentation: Kyoto
• [Presentation] Dephosphorylation of ERK by MKP-1 stimulates beta-and gamma-ENaC mRNA expression of renal A6 cells in hypotonic stress. (2009)
  • Author(s): Niisato N, Ohta M, Marunaka Y.
  • Organizer: 40th NIPS International symposium-International Joint symposium : PAT-CVR 2009
  • Place of Presentation: Okazaki
• [Presentation] MKP1-DEPENDENT ERK KEPHOSPHORYLATION SYIMULATES Na^+ REABSORPTION THROUGH BETA-AND GAMMA-ENaC EXPRESSION IN HYPOTONIC STRESS. (2009)
  • Author(s): Niisato N, Ohta M, Marunaka Y.
  • Organizer: 36TH INTERNATIONAL CONGRESS OF PHYSIOLOGICAL SCIENCES.
  • Place of Presentation: Kyoto
• [Presentation] MODIFICATION OF OSMOREGULATED ENaC TRAFFICKING BY ALDOSTERONE AND PROTEASE INHIBITOR. (2009)
  • Author(s): Nagai T, Niisato N, Marunaka Y.
  • Organizer: 36TH INTERNATIONAL CONGRESS OF PHYSIOLOGICAL SCIENCES.
  • Place of Presentation: Kyoto
• [Presentation] SODIUM HYDROGEN EXCHANGER ISOFORM1 (NHE-1)-DEPENDENT PROLIFERATION OF GASTRIC CANCER CELLS. (2009)
  • Author(s): Hosogi S, Takemura Y, Miyazaki H, Niisato N, Iwasaki Y, Marunaka Y.
  • Organizer: 36TH INTERNATIONAL CONGRESS OF PHYSIOLOGICAL SCIENCES.
  • Place of Presentation: Kyoto
• [Presentation] INTRACELLULAR CHLORIDE REGULATES CELL PROLIFERATION OF ANDROGEN-INDEPENDENT PROSTATE CANCER PC3 CELL. (2009)
  • Author(s): Hiraoka K, Miyazaki H, Niisato N, Marunaka Y.
  • Organizer: 36TH INTERNATIONAL CONGRESS OF PHYSIOLOGICAL SCIENCES.
  • Place of Presentation: Kyoto
• [Presentation] INTRACELLULAR CHLORIDE-DEPENDENT MECHANISM OF ACTIVATION OF STRESS-ACTIVATED PROTEIN KINASED IN HUMAN GASTRIC CANCER MKN28 CELLS. (2009)
  • Author(s): Ohsawa R, Miyazaki H, Otsuji E, Niisato N, Marunaka Y.
  • Organizer: 36TH INTERNATIONAL CONGRESS OF PHYSIOLOGICAL SCIENCES.
  • Place of Presentation: Kyoto
• [Presentation] INTRACELLULAR CHLORIDE REGULATES THE G1/S CELL CYCLE CHECKPOINT IN HUMAN GASTRIC CANCER MKN28 CELLS. (2009)
  • Author(s): Miyazaki H, Ohsawa R, Niisato N, Marunaka Y.
  • Organizer: 36TH INTERNATIONAL CONGRESS OF PHYSIOLOGICAL SCIENCES.
  • Place of Presentation: Kyoto
• [Presentation] THE ROLE OF CHLORIDE ION IN DYNAMICS OF MICROTUBULE. (2009)
  • Author(s): Nakajima K, Niisato N, Marunaka Y.
  • Organizer: 36TH INTERNATIONAL CONGRESS OF PHYSIOLOGICAL SCIENCES.
  • Place of Presentation: Kyoto
• [Presentation] ヒト気道上皮細胞におけるクロライドイオン分泌のフラボノイドによる制御機構;アルドステロンおよび浸透圧の上皮型ナトリウムチャネル細胞内局在修飾作用 (2009)
  • Author(s): 丸中良典, 新里直美
  • Organizer: 日本膜学会第31年会
  • Place of Presentation: 東京
• [Presentation] 低浸透圧刺激は、MKP1の発現制御によりENaCを介するNa+再吸収を亢進する (2009)
  • Author(s): 新里直美, 西尾恭介, 丸中良典
  • Organizer: 第52回日本腎臓学会学術総会
  • Place of Presentation: 横浜
• [Presentation] フラボノイドによる神経再生の試み (2009)
  • Author(s): 丸中良典, 新里直美
  • Organizer: 第12回研究報告会
  • Place of Presentation: 大阪
• [Presentation] クロライドイオン輸送体を分子標的とするアンドロゲン非依存性前立腺癌細胞増殖制御の試み (2009)
  • Author(s): 平岡健児, 沖原宏治, 浮村理, 高羽夏樹, 水谷陽一, 河内明宏, 宮崎裕明, 新里直美, 丸中良典, 三木恒治
  • Organizer: 第97回日本泌尿器科学会総会
  • Place of Presentation: 岡山
• [Presentation] 神経突起伸長におけるクロライドイオン輸送体NKCC1の役割-フラボノイドによる神経再生の試み- (2009)
  • Author(s): 中島謙一、新里直美、丸中良典
  • Organizer: 生理研研究会「上皮組織における細胞外環境感受機構」
  • Place of Presentation: 岡崎
• [Presentation] アルドステロンによるNa+再吸収亢進はp38を介する (2009)
  • Author(s): 太田麻利子、新里直美、丸中良典
  • Organizer: 生理研研究会「上皮組織における細胞外環境感受機構」
  • Place of Presentation: 岡崎
• [Presentation] Aldosterone modifies osmoregulated ENaC trafficking (2008)
  • Author(s): 丸中良典, 新里直美, 樽野陽幸, 西尾恭介
  • Organizer: 日本腎臓学会学術総会
  • Place of Presentation: 福岡
  • Year and Date: 2008-05-31
• [Presentation] Abnormal regulation of ENaC and SGK1 by aldosterone in Dahl salt-sensitive hypertension rat (2008)
  • Author(s): 新里直美, 宮崎裕明, 西尾恭介, 丸中良典
  • Organizer: 日本腎臓学会学術総会
  • Place of Presentation: 福岡
  • Year and Date: 2008-05-31
• [Presentation] Dietary sodium induces absormal espression of ENaC and SGK1 mRNA in Dahl salt-sensitively hypertensive rat (2008)
  • Author(s): 新里直美, 宮崎裕明, 西尾恭介, 丸中良典
  • Organizer: 日本腎臓学会学術総会
  • Place of Presentation: 福岡
  • Year and Date: 2008-05-31
• [Presentation] Nongenomic osmoregulation of ENaC-mediated sodium reabsorption (2008)
  • Author(s): 樽野陽幸, 新里直美, 西尾恭介, 丸中良典
  • Organizer: 日本腎臓学会学術総会
  • Place of Presentation: 福岡
  • Year and Date: 2008-05-31
• [Presentation] Osmoregulation of ENaC-mediated renal sodium reabsorption via genomic mechanisms (2008)
  • Author(s): 樽野陽幸, 新里直美, 西尾恭介, 丸中良典
  • Organizer: 日本腎臓学会学術総会
  • Place of Presentation: 福岡
  • Year and Date: 2008-05-31
• [Remarks]
  • URL: http://kpum-molecular-cell-physiology.info/index.html