Functions of myeloid-lectin receptors

• Project/Area Number: 20390109
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Kyoto University
• Principal Investigator: INABA Kayo Kyoto University, 大学院・生命科学研究科, 教授 (00115792)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000); Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2008: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: 動物 / 細胞 / 分子 / 炎症 / レクチン / マクロファージ / 樹状細胞 / C型レクチン / 好中球 / 肝炎 / 表現型 / 活性化 / 細胞分化 / 機能 / 単球 / リンパ器官 / 皮膚

Research Abstract

We generated SIGNR3-specific mAbs and investigated SIGNR3 expression in vivo. SIGNR3 was expressed on a vast majority of CD11b^+CD11c^<low> myeloid cells in various tissues, such as blood, dermis, lung, spleen and lymph nodes. By the cell transfer experiments using fluorescenated Ly6C^<high>SIGNR3^- bone marrow monocytes, SIGNR3 expression was upregulated along with the decrease in Ly6C expression during the circulation. Upon arrival at the peripheral LNs through HEV, the inoculated cells differentiated into CD11b^+ DCs in a couple of days. These results indicate that some DCs, especially in the subcutaneous lymph nodes, are possibly replenished by Ly6C^<high> monocytes.
DCIR1 was detected mainly intracellularly in various cell types, but transferred onto the cell surface by the activation with LPS and TNF-α.

Research Products

• [Journal Article] C-type lectin SIGNR1 enhances cellular oxidative burst responses against C. albicans in cooperation with Dectin-1. (2011)
  • Author(s): Takahara, K., Tokieda, T., Nagaoka, K., Takeda, T., Kimura, Y., Inaba.K.
  • Journal Title: Eur.J. Immunol. Feb24 (Epub ahead of print)
• [Journal Article] C-type lectin SIGNR1 enhances cellular oxidative burst responses against C.albicans in cooperation with Dectin-1 (2011)
  • Author(s): Kazuhiko Takahara
  • Journal Title: European Journal of Immunology Volume: 41(予定)(Feb 24 ahead of print)
  • Peer Reviewed
• [Journal Article] Expression of the C-type lectin, SIGNR3, on subsets of dendritic cells, macrophages and monocytes using a new monoclonal antibody. (2010)
  • Author(s): Nagaoka, K., Takahara, K., Minamino, K., Takeda, T., Yoshida, Y., Inaba, K.
  • Journal Title: J Leu.Biol. 88(5) Pages: 913-924
  • Peer Reviewed
• [Journal Article] Rho-mDial pathway is required for adhesion, migration, and T cell stimulation in dendritic cells. (2010)
  • Author(s): Tanizaki, H., Egawa, G., Inaba, K., Honda, T., Nakajima, S., Sagita Moniaga, C., Otsuka, A., Ishizaki, T., Tomura, M., Watanabe, T., Miyachi, Y., Narumiya, S., Okada, T., Kabashima, K.
  • Journal Title: Blood 116(26) Pages: 5875-5884
  • Peer Reviewed
• [Journal Article] Ly49Q plays a crucial role in neutrophil polarization and migration by regulating raft trafficking. (2010)
  • Author(s): Sasawatari, S., Yoshizaki, M., Taya, C., Tazawa, A., Furuyama-Tanaka, K., Yonekawa, H., Dohi, T., Makrigiannis, A.P., Sasazuki, T., Inaba, K., Toyama-Sorimachi, N.
  • Journal Title: Immunity 31(2) Pages: 200-213
  • Peer Reviewed
• [Journal Article] Expression of the C-type lectin, SIGNR3, on subsets of dendritic cells, macrophages and monocytes using a new monoclonal antibody. (2010)
  • Author(s): Koji Nagaoka
  • Journal Title: Journal of Luekocyte Biology Volume: 88 Pages: 913-924
  • Peer Reviewed
• [Journal Article] Invariant NKT cell anergy is induced by a strong TCR-mediated signal plus costimulation (2010)
  • Author(s): Tomoniri Iyoda
  • Journal Title: International Immunology Volume: 22 Pages: 905-913
  • Peer Reviewed
• [Journal Article] Rho-mDial pathway is required for adhesion, migration, and T cell stimulation in dendritic cells (2010)
  • Author(s): Hideaki Tanizaki
  • Journal Title: Blood Volume: 116 Pages: 5875-5884
  • Peer Reviewed
• [Journal Article] Ly49Q plays a crucial role in neutrophil polarization and migration by regulating raft trafficking (2010)
  • Author(s): Shigemi Sasawatari, et al.
  • Journal Title: Immunity Volume: 31 Pages: 200-213
  • Peer Reviewed
• [Journal Article] Dual role for Id2 in chemical carcinogen-induced skin tumorigenesis. (2009)
  • Author(s): Tokuriki, A., Iyoda, T., Inaba, K., Ikuta, K., Fijimoto, S., Kumakiri, M., Yokota, Y.
  • Journal Title: Carcinogenesis. 30(9) Pages: 1645-1650
  • Peer Reviewed
• [Journal Article] Dual role for Id2 in chemical carcinogen-induced skin tumorigenesis (2009)
  • Author(s): Atsushi Tokuriki, et al.
  • Journal Title: Carcinogenesis Volume: 30 Pages: 1645-1650
  • Peer Reviewed
• [Journal Article] Spatiotemporal regulation of intracellular trafficking of TLR9 by an inhibitory receptor, Ly49Q (2009)
  • Author(s): Mariko Yoshizaki, et al.
  • Journal Title: Blood Volume: 114 Pages: 1518-1527
  • Peer Reviewed
• [Journal Article] CD8^+CD205^+splenic dendritic cells are specialized to induce Foxp3^+ regulatory T cells. (2008)
  • Author(s): Yamazaki,S., Dudziak, D., Heidkamp,F.D., Fiorese, C., Bonito, A.J., Inaba,K., Nussenzweig, M.D., Steinman, R.M.
  • Journal Title: J.Immunol. 181(10) Pages: 6923-6933
  • Peer Reviewed
• [Journal Article] CD8^+CD205^+ splenic dendritic cells are specialized to induce Foxp3^+regulatory T cells. (2008)
  • Author(s): Yamazaki, S., Dudziak D., Heidkamp, GF., Fiorese, C., Bonito, AJ., Inaba, K., Nussenzweig, MC., Steinman, RM.
  • Journal Title: J.Immunol 181(10) Pages: 6923-6933
  • Peer Reviewed
• [Journal Article] Serial assessment of immune status by circulating CD8^+effector T cell frequencies for posttransplant infectious complications. (2008)
  • Author(s): Umenoto, S., Ozawa, K., Egawa, H., Takada, Y., Sato, H., Teramukai, S., Kasahara, M., Ofura, K., Ono, M., Takai, K., Fukushima, M., Inaba, K., Tanaka, K.
  • Journal Title: Clin.Dev.Immunol.
  • Peer Reviewed
• [Journal Article] Dendritic cells derived from thioredoxin binding protein-2 deficient mice attenuate Th1 responses. (2008)
  • Author(s): Son, A., Nakamura, H., Oka, S-I., Takenaka, M., Yoshihara, E., Liu, W.m Matsuo, Y., Kondo, N., Masutani, H., Ishii, Y., Iyoda, T., Inaba, K., Yodoi, J.
  • Journal Title: Eur.J.Immunol. 38(5) Pages: 1358-1367
  • Peer Reviewed
• [Journal Article] Dendritic cells derived from thioredoxin binding protein-2 deficient mice attenuate Th1 responses (2008)
  • Author(s): Aoi Son
  • Journal Title: Eur. J. Immunol. 38 Pages: 1358-1367
  • Peer Reviewed
• [Journal Article] Serial assessment of immune status by circulating CD8^+ effector T cell frequencies for posttransplant inferctions complications (2008)
  • Author(s): Uemoto Shinji
  • Journal Title: Clin. Dev. Immunol. 2008 Pages: 718386-718399
  • Peer Reviewed
• [Journal Article] CD8+ CD205+ splenic dendritic cells are specialized to induce Foxp3+ regulatory T cells (2008)
  • Author(s): Sayuri Yamazaki
  • Journal Title: J. Immunol. 181 Pages: 6923-6933
  • Peer Reviewed
• [Journal Article] Invariant NKT cell anergy is induced by a strong TCR-mediated signal plus costimulation.
  • Author(s): Iyoda, T., Ushida, M., Kimura, Y., Minamino, K., Hayuka, A., Yokohata, S., Ehara, H., Kayo Inaba, K.
  • Journal Title: Int.Immunol. 22(11) Pages: 905-913
  • Peer Reviewed
• [Presentation] Recognition and cellular function of SIGNR1 in response to C.albicans in situ (2010)
  • Author(s): Kazuhiko Takahara
  • Organizer: The 10^<th> Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路夢舞台(兵庫県)
  • Year and Date: 2010-09-08
• [Presentation] Ly6C^<high> SIGNR3^- monocytes can differentiate to CD11b^+ dendritic cells in subcutaneous lymph nodes under steady-state condition (2010)
  • Author(s): Koji Nagaoka
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: The Kobe Convention Center Complex(兵庫県)
  • Year and Date: 2010-08-25
• [Presentation] Regulation of B cell function and development through IRF-2 in type I interferon receptor-dependent and-independent mechanisms (2010)
  • Author(s): Kento Minamino
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: The Kobe Convention Center Complex(兵庫県)
  • Year and Date: 2010-08-24
• [Presentation] Role of conventional dendritic cell subsets in the presentation of α-galactosylceramide to invariant NKT cells in vivo (2010)
  • Author(s): Maki Ushida
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: The Kobe Convention Center Complex(兵庫県)
  • Year and Date: 2010-08-24
• [Presentation] Binding property of SIGNR1 against surface mannoproteins of Candia albicans (2010)
  • Author(s): Sumika Tokieda
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: The Kobe Convention Center Complex(兵庫県)
  • Year and Date: 2010-08-24
• [Presentation] Invariant NKT cell anergy is induced by strong TCR-mediated signal with co-stimulation (2010)
  • Author(s): Tomoniri Iyoda
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: The Kobe Convention Center Complex(兵庫県)
  • Year and Date: 2010-08-23
• [Presentation] 樹状細胞-基礎から臨床へ- (2010)
  • Author(s): 稲葉カヨ
  • Organizer: 第11回国際統合医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-18
• [Presentation] 樹状細胞-基礎から臨床へ (2010)
  • Author(s): 稲葉カヨ
  • Organizer: 第11回国際統合医学会学術集会
  • Place of Presentation: 東京コンファレンスセンター品川(東京都)(招待講演)
  • Year and Date: 2010-07-18
• [Presentation] Is SIGNR3 a new marker to identify intermeidated cell type differentiating into dendritic cells? (2010)
  • Author(s): 稲葉カヨ
  • Organizer: In "Asia Pacific Regional Meeting"
  • Place of Presentation: Osaka
  • Year and Date: 2010-02-04
• [Presentation] 樹状細胞研究の動向と展望 (2010)
  • Author(s): 稲葉カヨ
  • Organizer: 第9回リウマチ性疾患研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-01-23
• [Presentation] Role of SIGNR1 molecules in innate immune responses. (2010)
  • Author(s): Inaba K., Takahara, K.
  • Organizer: In "Adaptive and Innate Immune Responses to Neglected Tropical Diseases Conference".
  • Place of Presentation: San Diego, Ca.
• [Presentation] Role of SIGNR1 mocleuls in innate immune responses (2010)
  • Author(s): Kayo Inaba
  • Organizer: Adaptive and Innate Immune Responses to Neglected Tropical Diseases
  • Place of Presentation: San Diego (USA)
• [Presentation] CD8+ CD205+ 脾樹状細胞サブセットと Foxp3+ 制御性T細胞の誘導 (2009)
  • Author(s): 山崎小百合, et al.
  • Organizer: 第39回日本免疫学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2009-12-02
• [Presentation] Invariant NKT cell anergy can be induced via a PD-1 independent manner. (2009)
  • Author(s): Tomonori Iyoda, et al.
  • Organizer: 第39回日本免疫学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2009-12-02
• [Presentation] A crucial role of Ly49Q-mediated dynamic regulation of lipid rafts in neutrophils (2009)
  • Author(s): Shigeo Sasawatari, et al.
  • Organizer: 第39回日本免疫学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2009-12-01
• [Presentation] Determination of polysaccharides on C.albicans mannoprotein recognized by human C-type lectin, DC-SIGN. (2009)
  • Author(s): Kazuhiko Takahara, et al.
  • Organizer: 9th Awaji International Forum for Infection and Immunity
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫県)
  • Year and Date: 2009-09-08
• [Presentation] 樹状細胞研究の動向・展望と臨床応用への課題 (2009)
  • Author(s): 稲葉カヨ
  • Organizer: 第84回北野学術講演会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-25
• [Presentation] 樹状細胞 (2009)
  • Author(s): 稲葉カヨ
  • Organizer: 日本免疫学会サマースクール2009
  • Place of Presentation: 淡路
  • Year and Date: 2009-07-17
• [Presentation] CD8^+ CD205^+ splenic dendritic cells are specialized to induce Foxp3^+ regulatory T cells (2009)
  • Author(s): Sayuri Yamazaki, et al.
  • Organizer: 第74回日本インターフェロン・サイトカイン研究会
  • Place of Presentation: 京都大学医学部芝蘭会館(京都府)
  • Year and Date: 2009-06-26
• [Presentation] DC2008をふり返って (2009)
  • Author(s): 稲葉カヨ
  • Organizer: 第108回日本皮膚科学会学術集会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2009-04-24
• [Presentation] Role of SIGN-related molecules in innate immune responses (2009)
  • Author(s): Kazuhiko Takahara
  • Organizer: International Symposium "Acquired Immunity and Glycobiology
  • Place of Presentation: かずさ
  • Year and Date: 2009-03-24
• [Presentation] 樹状細胞:免疫応答制御の視点から (2009)
  • Author(s): 稲葉カヨ
  • Organizer: 第5回千葉基礎・臨床免疫セミナー
  • Place of Presentation: 千葉
  • Year and Date: 2009-01-09
• [Presentation] Determination of polysaccharides on C.albicans mannoprotein recognized by human C-type lectin, DC-SIGN. (2009)
  • Author(s): Takahara, K., Arita, T., Shibata, N., Okawa, Y, Inaba K.
  • Organizer: In "The 9^<th> Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji Yumebutai, Japan
• [Presentation] Role of SIGN-related molecules in innate immune responses. (2009)
  • Author(s): Takahara, K., Inaba K.
  • Organizer: International Symposium "Acquired Immunity and Glycobiology"
  • Place of Presentation: Kazusa, Chiba
• [Presentation] Recognition property of SIGNRl for C. albicans mannoprotein to enhance TNF-α production and candicidal activity (2008)
  • Author(s): Kazuhiko Takahara
  • Organizer: 第38回日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] C-type lectins expressed on macrophages and dendritic cell subsets (2008)
  • Author(s): Kayo Inaba
  • Organizer: The 57th Fall Conference of the Korean Association of Immunobiologists
  • Place of Presentation: Seoul
  • Year and Date: 2008-11-14
• [Presentation] Subsets of macrophages and dendritic cells defined by newly established monoclonal antibody against SIGNR3 (2008)
  • Author(s): Kayo Inaba
  • Organizer: the 8th Awaji International Forum for Infection and Immunity
  • Place of Presentation: 淡路島
  • Year and Date: 2008-09-10
• [Presentation] Role of SIGNRl in anti-microbial responses (2008)
  • Author(s): Kayo Inaba
  • Organizer: US-Japan Cooperative Medical Science Program Aging & Immunosenescence Joint Meeting and Workshop
  • Place of Presentation: San Francisco
  • Year and Date: 2008-06-19
• [Presentation] マウスC型レクチンSIGNRファミリー分子の機能 (2008)
  • Author(s): 稲葉カヨ
  • Organizer: 第48回日本リンパ網内系学会
  • Place of Presentation: 大阪
  • Year and Date: 2008-06-13
• [Presentation] C-type lectins expressed on macrophages and dendritic cells. (2008)
  • Author(s): Inaba K., Takahara, K.
  • Organizer: In the 57^<th> Fall conference of the Korean Association of Immunologist.
  • Place of Presentation: Seoul, Korea
• [Presentation] Subsets of macrophages and dendritic cells defined by newly established monoclonal antibody against SIGNR3. (2008)
  • Author(s): 稲葉カヨ
  • Organizer: In 8^<th> Awaji International Forum for Infection and Immunity
  • Place of Presentation: Awaji Island, Hyogo
• [Presentation] Role of SIGNR1 in anti-microbial responses. (2008)
  • Author(s): Inaba K., Takahara, K.
  • Organizer: In U.S.-Japan Cooperative Medical Science Program : Workshop on Aging&Immunosenescence
  • Place of Presentation: San Francisco, CA
• [Presentation] Preferential migration of Ly6C^<high> SIGNR3^+monocyte subset into peripheral lympb nodes in the teadvv state (2008)
  • Author(s): Koji Nagaoka
  • Organizer: 第38日本免疫学会学術集会
  • Place of Presentation: 京都
• [Book] 樹状細胞研究のトピックス『炎症と免疫』特集IVol.18, No.5. (2010)
  • Author(s): 稲葉カヨ
  • Publisher: 先端医学社
• [Book] 炎症と免疫特集I(編集)(樹状細胞研究のトピックス) (2010)
  • Author(s): 稲葉カヨ
  • Publisher: 先端医学社
• [Book] Isolation of Dendritic Cells. Aug ; Chapter 3 : Unit3.7 (2009)
  • Author(s): Inaba K., Swiggard, W.J., Steinman, R.M., Romani, N., Schuler, G., Brinster, C.
  • Publisher: Curr Protoc Immunol.
• [Book] 樹状細胞『炎症・再生医学事典』 (2009)
  • Author(s): 稲葉カヨ
  • Publisher: 朝倉書店
• [Book] 生体における樹状細胞の前駆細胞とその分化『肝胆膵』Vol.58, No.2 (2009)
  • Author(s): 長岡孝治、稲葉カヨ
  • Publisher: アークメディア
• [Book] 樹状細胞:発見・同定の歴史を今に見る『実験医学・増刊:樹状細胞による免疫制御と臨床応用』 (2008)
  • Author(s): 稲葉カヨ
  • Publisher: 羊土社
• [Book] 第7章抗原提示「免疫学イラストレイテッド原著第7版」(訳) (2008)
  • Author(s): 稲葉カヨ
• [Book] 生体における樹状細胞の前駆細胞とその分化 (2008)
  • Author(s): 長岡孝治
  • Total Pages: 10
  • Publisher: アークメディア
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