A novel immune regulation mechanism by Ca-independent PLA2 and its contribution to neurodegenerative diseases

• Project/Area Number: 20390117
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: St.Marianna University School of Medicine
• Principal Investigator: SEINO Ken-Ichiro St.Marianna University School of Medicine, 遺伝子病制御研究所, 教授 (20312845)
• Research Collaborator: WADA Haruka 北海道大学, 遺伝子病制御研究所, 助教 (70392181) ; KOJO Satoshi 北海道大学, 遺伝子病制御研究所, 助教 (70360542)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2008: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
• Keywords: 疾患モデル動物 / NKT細胞 / INAD

Research Abstract

We have investigated the mutant mice bearing a point mutation in Ca-independent PLA2 (iPLA2・、Pla2g6) . They showed a progressive paralysis in their lower limbs since several weeks after the birth. The mutant mice were realized as model mice of human infantile neuroaxonal dystrophy. The mice also showed some immune abnormality such as increase of NKT cell population. We can use the mutant mice for further investigating the disease mechanism and developing new therapeutic strategies.

Research Products

• [Journal Article] Successful differentiation to T cells, but unsuccessful B-cell generation, from B-cell-derived induced pluripotent stem cells. (2011)
  • Author(s): Wada H, Kojo S, Kusama C, Okamoto N, Sato Y, Ishi zuka B, Seino K.
  • Journal Title: Int Immunol Volume: 23 Pages: 65-74
  • NAID: 10030575944
  • Peer Reviewed
• [Journal Article] Establishment of an improved mouse model for infantile neuroaxonal dystrophy bearing a point mutation in Pla2g6 which shows early disease onset. (2009)
  • Author(s): Wada H, Yasuda T, Miura I, Watabe K, Sawa C, Kamijuku H, Kojo S, Taniguchi M, Nishino I, Wakana S, Yoshida H, Seino K.
  • Journal Title: Am J Pathol 175 Pages: 2257-63
  • Peer Reviewed
• [Journal Article] Establishment of an improved mouse model for infantile neuroaxonal dy strophy bearing a point mutation in Pla2g6 which shows early disease onset. (2009)
  • Author(s): Wada H, et.al
  • Journal Title: Am J Pathol 175 Pages: 2257-63
  • Peer Reviewed
• [Presentation] Successful differentiation to T cells, but unsuccessful B-cell generation, from B-cell-derived induced pluripotent stem cells. (2010)
  • Author(s): Wada H, Seino K.
  • Organizer: Natural Immunity & NK2010
  • Place of Presentation: DUBROVNIK, Croatia
  • Year and Date: 2010-09-14
• [Presentation] ENU mutagenesisによるヒト乳児型軸索ジストロフィーモデルマウスの樹立 (2009)
  • Author(s): 渡部和彦, 他
  • Organizer: 第41回日本臨床分子形態学会総会・学術集会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2009-09-04
• [Presentation] Establishment of a mouse mutant bearing a point mutation in Pla2g6 which show early onset of neuroaxonal dystrophy. (2009)
  • Author(s): Wada H, et.al
  • Organizer: A_2 and Lipid Mediators PLM2009
  • Place of Presentation: 国立情報学研究所(東京都)
  • Year and Date: 2009-05-26
• [Presentation] Establishment of a mouse mutant bearing a point mutation in Pla2g6 which show early onset of neuroaxonal dystrophy. (2009)
  • Author(s): Wada H, Yasuda T, Sawa C, Kamijuku H, Watabe K, Yoshida H, Seino K.
  • Organizer: Phospholipase A_2 and Lipid Mediators PLM2009
  • Year and Date: 2009-05-25
• [Presentation] ENUランダムミュータジェネシスによる神経系および免疫系に変調をきたすマウス系統の樹立と解析 (2008)
  • Author(s): 和田 はるか
  • Organizer: 日本免疫学会総会
  • Place of Presentation: 国立京都国際会館
• [Remarks] ホームプージ等