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Analysis and its significance of multifunction of staphylococcal enterotoxin family

Research Project

Project/Area Number 20390122
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionHirosaki University

Principal Investigator

NAKANE Akio  弘前大学, 大学院・医学研究科, 教授 (30164239)

Co-Investigator(Kenkyū-buntansha) HU Dong-liang  弘前大学, 大学院・医学研究科, 准教授 (10333733)
Co-Investigator(Renkei-kenkyūsha) NARITA Kouji  弘前大学, 大学院・医学研究科, 助教 (30419220)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2008: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Keywordsブドウ球菌エンテロトキシン / TSST-1 / SEA / オートファジー / IL-17 / 黄色ブドウ球菌 / 感染 / スーパー抗原 / 嘔吐活性 / ブドウ球菌エンテロトキシ / 免疫
Research Abstract

We investigated roles of staphylococcal enterotoxin family molecules in infection, emesis and diarrhea.
(1) TSST-1 played an important role in pathogenesis of Staphylococcus aureus infectio
(2) Among SEA, SEC and TSST-1, SEA and SEC showed emesis-inducing activity but none of them revealed diarrhea-inducing activity.
(3) TSST-1 suppressed autophagy formation in epithelial cells.
(4) Vaccination with recombinant clumping factor A induced acquired host resistance to S. aureus infection in mice, depending on IL-17.

Report

(5 results)
  • 2010 Annual Research Report   Self-evaluation Report ( PDF )   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (47 results)

All 2011 2010 2009 2008 Other

All Journal Article (20 results) (of which Peer Reviewed: 20 results) Presentation (23 results) Remarks (4 results)

  • [Journal Article] Superantigenic activity of toxic shock syndrome toxin-1 is resistant to heating and digestive enzymes2011

    • Author(s)
      Li S-J, Hu D-L, Maina EK, Shinagawa K, Omoe K, Nakane A
    • Journal Title

      J Appl Microbiol

      Volume: 110(3) Pages: 729-736

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of interleukin-17A in cell-mediated protection against Staphylococcus aureus infection in mice immunized with the fibronectin-binding domain of clumping factor2010

    • Author(s)
      Narita K, Hu D-L, Mori F, Wakabayashi K, Iwakura Y, Nakane A
    • Journal Title

      Infect Immun

      Volume: 78(10) Issue: 10 Pages: 4234-4242

    • DOI

      10.1128/iai.00447-10

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] High affinity of interaction between superantigen and TCRVss molecules induces a high level and prolonged expansion of superantigen-reactive CD4^+ T cells2010

    • Author(s)
      Omoe K, Nunomura W, Kato H, Li Z-J, Igarashi O, Araake M, Sano K, Ono HK, Abe Y, Hu D-L, Nakane A, Kiyono H, Takakura Y, Shinagawa K, Uchiyama T, Imanishi KI
    • Journal Title

      J Biol Che

      Volume: 285(40) Issue: 40 Pages: 30427-30435

    • DOI

      10.1074/jbc.m110.140871

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protective effect of glutathione S-transferase-fuzed mutant staphylococcal enterotoxin C against Staphylococcus aureus-induced bovine mastitis2010

    • Author(s)
      Cui J-C, Zhang B-J, Lin Y-C, Wang Q-K, Qian A-D, Nakane A, Hu D-L, Tong G-Z
    • Journal Title

      Vet Immunol Immunopath

      Volume: 135(1-2) Pages: 64-70

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] High affinity of interaction between superantigen and TCRVβ molecules induces a high level and prolonged expansion of superantigen-reactive CD4^+ T cells2010

    • Author(s)
      Omoe K, Nunomura W, Kato H, Li Z-J, Igarashi O, Araake M, Sano K, Ono HK, Abe Y, Hu D-L, Nakane A, Kiyono H, Takakura Y, Shinagawa K, Uchiyama T, Imanishi KI.
    • Journal Title

      J Biol Chem 285

      Pages: 30427-30435

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Role of interleukin-17A in cell-mediated protection against Staphylococcus aureus infection in mice immunized with the fibronectin-binding domain of clumping factor2010

    • Author(s)
      Narita K, Hu D-L, Mori F, Wakabayashi K, Iwakura Y, Nakane A.
    • Journal Title

      Infect Immun 78

      Pages: 4234-4242

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Role of interleukin-17A in cell-mediated protection against Staphylococcusaureus infection in mice immunized with the fibronectin-binding domain of clumping factor2010

    • Author(s)
      Narita K, Hu D-L, Nakane A, et al.
    • Journal Title

      Infection and Immunity

      Volume: 78 Pages: 4234-4242

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High affinity of interaction between superantigen and TCRVβ molecules induces a high level and prolonged expansion of superantigen-reactive CD4^+ T cells2010

    • Author(s)
      Omoe K, Hu D-L, Nakane A, et al.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 285 Pages: 30427-30435

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protective effect of glutathione S-transferase-fuzed mutant staphylococcal enterotoxin C against Staphylococcus aureus-induced bovine mastitis2010

    • Author(s)
      Cui J-C, Hu D-L, Nakane A, et al.
    • Journal Title

      Veterinary Immunology and Immunopathology

      Volume: 135 Pages: 64-70

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] c-di-GMP as vaccine adjuvant enhances protection against systemic methicillin-resistant2009

    • Author(s)
      Hu D-L, Narita K, Hyodo M, Hayakawa Y, Nakane A, Karaolis DKR
    • Journal Title

      Staphylococcus aureus (MRSA) infection

      Volume: 27(35) Issue: 35 Pages: 4867-4873

    • DOI

      10.1016/j.vaccine.2009.04.053

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunization with a nontoxic mutant of staphylococcal enterotoxin A, SEA D227A, protects against enterotoxin-induced emesis in house musk shrews2009

    • Author(s)
      Hu D-L, Omoe K, Sashinami H, Shinagawa K, Nakane A
    • Journal Title

      J Infect Dis

      Volume: 199(3) Issue: 3 Pages: 302-310

    • DOI

      10.1086/596065

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] c-di-GMP as vaccine adjuvant enhances protection against systemic methicillin-resistant Staphylococcus aureus (MRSA) infection2009

    • Author(s)
      Hu D-L, Narita K, Hyodo M, Hayakawa Y, Nakane A, Karaolis DKRInfect Immun
    • Journal Title

      Vaccine 27

      Pages: 4867-4873

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Immunization with a nontoxic mutant of staphylococcal enterotoxin A, SEA D227A, protects against enterotoxin-inducedemesis in house musk shrews2009

    • Author(s)
      Hu D-L, Omoe K, Sashinami H, Shinagawa K, Nakane A.
    • Journal Title

      J Infect Dis 199

      Pages: 302-310

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] c-di-GMP as vaccine adjuvant enhances protection against systemic methicillin-resistant Staphylococcus aureus(MRSA)infection2009

    • Author(s)
      Hu DL, Nakane A, et al.
    • Journal Title

      Vaccine 27

      Pages: 4867-4873

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunization with nontoxic mutant staphylococcal enterotoxin A, D227A, protects against enterotoxin-induced emesis in house musk shrews2009

    • Author(s)
      Hu DL, Nakane A, et.al.
    • Journal Title

      J. Infect. Dis 199

      Pages: 302-310

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Uchiyama T, Shinagawa K. Identification and characterization of two novel staphylococcal enterotoxins, types S and T2008

    • Author(s)
      Ono H, Omoe K, Imanishi K, Iwakabe Y, Hu D-L, Kato H, Saito N, Nakane A, Uchiyama T, Shinagawa K
    • Journal Title

      Infect Immun

      Volume: 76(11) Issue: 11 Pages: 4999-5005

    • DOI

      10.1128/iai.00045-08

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparative prevalence of superantigenic toxin genes in methicillin-resistant and methicillin-susceptible Staphylococcus aureus islates2008

    • Author(s)
      Hu D-L, Omoe K, Inoue F, Kasai T, Yasujima M, Shinagawa K, Nakane A
    • Journal Title

      J Med Microbiol

      Volume: 57(9) Issue: 9 Pages: 1106-1112

    • DOI

      10.1099/jmm.0.2008/002790-0

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparative prevalence of superantigenic toxin genes in methicillin-resistant and methicillin- susceptible Staphylococcus aureus isolates2008

    • Author(s)
      Hu D-L, Omoe K, Inoue F, Kasai T, Yasujima M, Shinagawa K, Nakane A.
    • Journal Title

      J Med Microbiol 57(9)

      Pages: 1106-1112

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Comparative Prevalence of Superantigenic Toxin Genes in Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolates2008

    • Author(s)
      Hu DL, Nakane A., et.al.
    • Journal Title

      J. Med. Microbiol 57

      Pages: 1106-1112

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization of two novel staphylocoCcAl enteroloxins types S and T2008

    • Author(s)
      Ono H. Hu DL, Nakane A, et.al.
    • Journal Title

      Infect. Immun 76

      Pages: 4999-5005

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Staphylococcal enterotoxin A is resistant to heating and digestive enzyme treatment on the emetic activity2010

    • Author(s)
      Hu D-L, Maina EK, Tomioka Y, Li SJ, Narita K, Osanai A, Shinagawa K, Omoe K, Nakane A
    • Organizer
      The12^<th>Meeting of Hirosaki International Forum of medical Science
    • Place of Presentation
      Hirosaki
    • Year and Date
      2010-10-29
    • Related Report
      2010 Final Research Report
  • [Presentation] The role of IL-17A in the cell-mediated immunity induced by an immunization with clumping factor A against Staphylococcus aureus infection2010

    • Author(s)
      Narita K, Hu D-L, Nakane A
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      Kobe
    • Year and Date
      2010-08-25
    • Related Report
      2010 Final Research Report
  • [Presentation] The superantigenic activity of toxic shock syndrome toxin-1 is resistant to heating and digestive enzyme treatment2010

    • Author(s)
      Hu DL, Maina EK, Tomioka Y, Li SJ, Narita K, Osanai A, Shinagawa K, Omoe K, Nakane A
    • Organizer
      14^<th> International Congress of Immunolog
    • Place of Presentation
      Kobe
    • Year and Date
      2010-08-25
    • Related Report
      2010 Final Research Report
  • [Presentation] Analysis of staphylococcal enterotoxin type A movement in gastrointestinal tract of house musk shrew and characterization of target cell of toxin2010

    • Author(s)
      Ono HK, Nishizawa M, Yamamoto Y, Hu DL, Nakane A, Omoe K
    • Organizer
      14^<th> International Congress of Immunology
    • Place of Presentation
      Kobe
    • Year and Date
      2010-08-25
    • Related Report
      2010 Final Research Report
  • [Presentation] The role of IL-17A in the cell-mediated immunity induced by an immunization with clumping factor A against Staphylococcus aureus infection2010

    • Author(s)
      Narita K, Hu D-L, Nakane A.
    • Organizer
      14^<th> International Congress of Immunology
    • Place of Presentation
      Kobe International Exhibition Hall, Kobe, Japan
    • Year and Date
      2010-08-25
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] The role of IL-17A in the cell-mediated immunity induced by animmunization with dumping factor A against Staphylococcus aureusinfection2010

    • Author(s)
      Kouji Narita
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      神戸国際展示場(神戸市)
    • Year and Date
      2010-08-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] c-di-GMP as a vaccine adjuvant enhances protection against Staphylococcus aureus infection2010

    • Author(s)
      Narita K, Hu D-L, Nakane A
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2010-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] The role of interleukin-17 in the protective effect of an immunization with clamping factor A2010

    • Author(s)
      Hu D-L, Narita K, Nakane A
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2010-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] c-di-GMP as a vaccine adjuvant enhances protection against Staphylococcus aureus infection2010

    • Author(s)
      Narita K, Hu D-L, Nakane A
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] The role of interleukin-17 in the protective effect of an immunization with clamping factor A2010

    • Author(s)
      Hu D-L, Narita K, Nakane A
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] C-di-GMP as a vaccine adjuvant enhances protection against Staphylococcus aureus infection2010

    • Author(s)
      胡東良、成田浩司、中根明夫
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      横浜パシフィコ(横浜市)
    • Year and Date
      2010-03-27
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] 無毒変異体ブドウ球菌エンテロトキシンAの免疫による嘔吐予防効果2009

    • Author(s)
      胡東良、重茂克彦、差波拓志、品川邦汎、中根明夫
    • Organizer
      第56回トキシンシンポジウム
    • Place of Presentation
      長良川
    • Year and Date
      2009-08-26
    • Related Report
      2010 Final Research Report
  • [Presentation] 無毒変異体ブドウ球菌エンテロトキシンAの免疫による嘔吐予防効果2009

    • Author(s)
      胡東良、重茂克彦、差波拓志、品川邦汎、中根明夫
    • Organizer
      第56回トキシンシンポジウム
    • Place of Presentation
      岐阜県長良川
    • Year and Date
      2009-08-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] A nontoxic mutant staphylococcal enterotoxin A protects against enterotoxin-induced emesis2009

    • Author(s)
      胡東良、重茂克彦、差波拓志、品川邦汎、中根明夫
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-12
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] A nontoxic mutant staphylococcal enterotoxin A protects against enterotoxin-induced emesis2009

    • Author(s)
      胡東良、重茂克彦、差波拓志、品川邦汎、中根明夫
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Year and Date
      2009-03-12
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] Vaccination with nontoxic mutant staphylococcal enterotoxin A, D227A, protects against enterotoxin-induced emesis in house musk shrews2008

    • Author(s)
      胡東良、重茂克彦、差波拓志、品川邦汎、中根明夫
    • Organizer
      第31回日本分子生物学会・第81回日本生化学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] クランピングファクターAによる免疫はIL-17産生を促進し黄色ブドウ球菌感染防御効果を示す2008

    • Author(s)
      成田浩司、胡 東良、中根明夫
    • Organizer
      第38回日本免疫学会総会
    • Place of Presentation
      京都
    • Year and Date
      2008-12-01
    • Related Report
      2010 Final Research Report
  • [Presentation] クランピングファクターAによる免疫はIL-17産生を促進し黄色ブトウ球菌感染防御効果を示す2008

    • Author(s)
      成田浩司、胡東良、中根明夫
    • Organizer
      第38回日本免疫学会総会
    • Place of Presentation
      京都
    • Year and Date
      2008-12-01
    • Related Report
      2008 Annual Research Report
  • [Presentation] Staphylococcal enterotoxin A induces emesis through increasing serotonin release in intestine and endogenous cannabinoid system protects against the emetic response2008

    • Author(s)
      Hu, D-L, Omoe K, Shinagawa K, Nakane A
    • Organizer
      13th International Symposium on Staphylococci & Staphylococcal Infections
    • Place of Presentation
      Carens, Australia
    • Year and Date
      2008-09-07
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] Staphylococcal enterotoxin A induces emesis through increasing serotonin release in intestine and endogenous cannabinoid system protects against emetic response2008

    • Author(s)
      Hu D-L, Omoe K, Shinagawa K, Nakane A.
    • Organizer
      13^<th> International Symposium on Staphylococci and Staphylococcal Infections
    • Place of Presentation
      Cairns Convention Centre, Cairns, Australia
    • Year and Date
      2008-09-07
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] ブドウ球菌エンテロトキシンの嘔吐誘導とその制御機序2008

    • Author(s)
      胡 東良、重茂克彦、品川邦汎、中根明夫
    • Organizer
      第2回細菌学・若手コロッセウム
    • Place of Presentation
      湘南
    • Year and Date
      2008-08-03
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] 黄色ブドウ球菌接着因子による感染予防効果2008

    • Author(s)
      成田浩司、胡 東良、重茂克彦、品川邦汎、中根明夫
    • Organizer
      第73回JSICR・第19回日本生体防御学会・第45回補体シンポジウム合同学会
    • Place of Presentation
      札幌
    • Year and Date
      2008-07-10
    • Related Report
      2010 Final Research Report
  • [Presentation] 黄色フドワ球函接看因チによる感染予防効果2008

    • Author(s)
      成田浩司、胡東良、重茂克彦、品川邦汎、中根明夫
    • Organizer
      第73回JSICR・第19回日本生体防御学会・第45回補体シンポジウム合同学会
    • Place of Presentation
      札幌
    • Year and Date
      2008-07-10
    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.bact.hirosaki-u.ac.jp/

    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ情報

    • URL

      http://www.bact.hirosaki-u.ac.jp/

    • Related Report
      2010 Self-evaluation Report
  • [Remarks]

    • URL

      http://www.bact.hirosaki-u.ac.jp/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.bact.hirosaki-u.ac.jp/

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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