Analysis and its significance of multifunction of staphylococcal enterotoxin family

• Project/Area Number: 20390122
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Hirosaki University
• Principal Investigator: NAKANE Akio 弘前大学, 大学院・医学研究科, 教授 (30164239)
• Co-Investigator(Kenkyū-buntansha): HU Dong-liang 弘前大学, 大学院・医学研究科, 准教授 (10333733)
• Co-Investigator(Renkei-kenkyūsha): NARITA Kouji 弘前大学, 大学院・医学研究科, 助教 (30419220)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000); Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2008: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
• Keywords: ブドウ球菌エンテロトキシン / TSST-1 / SEA / オートファジー / IL-17 / 黄色ブドウ球菌 / 感染 / スーパー抗原 / 嘔吐活性 / ブドウ球菌エンテロトキシ / 免疫

Research Abstract

We investigated roles of staphylococcal enterotoxin family molecules in infection, emesis and diarrhea.
(1) TSST-1 played an important role in pathogenesis of Staphylococcus aureus infectio
(2) Among SEA, SEC and TSST-1, SEA and SEC showed emesis-inducing activity but none of them revealed diarrhea-inducing activity.
(3) TSST-1 suppressed autophagy formation in epithelial cells.
(4) Vaccination with recombinant clumping factor A induced acquired host resistance to S. aureus infection in mice, depending on IL-17.

Research Products

• [Journal Article] Superantigenic activity of toxic shock syndrome toxin-1 is resistant to heating and digestive enzymes (2011)
  • Author(s): Li S-J, Hu D-L, Maina EK, Shinagawa K, Omoe K, Nakane A
  • Journal Title: J Appl Microbiol Volume: 110(3) Pages: 729-736
  • Peer Reviewed
• [Journal Article] Role of interleukin-17A in cell-mediated protection against Staphylococcus aureus infection in mice immunized with the fibronectin-binding domain of clumping factor (2010)
  • Author(s): Narita K, Hu D-L, Mori F, Wakabayashi K, Iwakura Y, Nakane A
  • Journal Title: Infect Immun Volume: 78(10) Issue: 10 Pages: 4234-4242
  • DOI: 10.1128/iai.00447-10
  • Peer Reviewed
• [Journal Article] High affinity of interaction between superantigen and TCRVss molecules induces a high level and prolonged expansion of superantigen-reactive CD4^+ T cells (2010)
  • Author(s): Omoe K, Nunomura W, Kato H, Li Z-J, Igarashi O, Araake M, Sano K, Ono HK, Abe Y, Hu D-L, Nakane A, Kiyono H, Takakura Y, Shinagawa K, Uchiyama T, Imanishi KI
  • Journal Title: J Biol Che Volume: 285(40) Issue: 40 Pages: 30427-30435
  • DOI: 10.1074/jbc.m110.140871
  • Peer Reviewed
• [Journal Article] Protective effect of glutathione S-transferase-fuzed mutant staphylococcal enterotoxin C against Staphylococcus aureus-induced bovine mastitis (2010)
  • Author(s): Cui J-C, Zhang B-J, Lin Y-C, Wang Q-K, Qian A-D, Nakane A, Hu D-L, Tong G-Z
  • Journal Title: Vet Immunol Immunopath Volume: 135(1-2) Pages: 64-70
  • Peer Reviewed
• [Journal Article] High affinity of interaction between superantigen and TCRVβ molecules induces a high level and prolonged expansion of superantigen-reactive CD4^+ T cells (2010)
  • Author(s): Omoe K, Nunomura W, Kato H, Li Z-J, Igarashi O, Araake M, Sano K, Ono HK, Abe Y, Hu D-L, Nakane A, Kiyono H, Takakura Y, Shinagawa K, Uchiyama T, Imanishi KI.
  • Journal Title: J Biol Chem 285 Pages: 30427-30435
  • Peer Reviewed
• [Journal Article] Role of interleukin-17A in cell-mediated protection against Staphylococcus aureus infection in mice immunized with the fibronectin-binding domain of clumping factor (2010)
  • Author(s): Narita K, Hu D-L, Mori F, Wakabayashi K, Iwakura Y, Nakane A.
  • Journal Title: Infect Immun 78 Pages: 4234-4242
  • Peer Reviewed
• [Journal Article] Role of interleukin-17A in cell-mediated protection against Staphylococcusaureus infection in mice immunized with the fibronectin-binding domain of clumping factor (2010)
  • Author(s): Narita K, Hu D-L, Nakane A, et al.
  • Journal Title: Infection and Immunity Volume: 78 Pages: 4234-4242
  • Peer Reviewed
• [Journal Article] High affinity of interaction between superantigen and TCRVβ molecules induces a high level and prolonged expansion of superantigen-reactive CD4^+ T cells (2010)
  • Author(s): Omoe K, Hu D-L, Nakane A, et al.
  • Journal Title: Journal of Biological Chemistry Volume: 285 Pages: 30427-30435
  • Peer Reviewed
• [Journal Article] Protective effect of glutathione S-transferase-fuzed mutant staphylococcal enterotoxin C against Staphylococcus aureus-induced bovine mastitis (2010)
  • Author(s): Cui J-C, Hu D-L, Nakane A, et al.
  • Journal Title: Veterinary Immunology and Immunopathology Volume: 135 Pages: 64-70
  • Peer Reviewed
• [Journal Article] c-di-GMP as vaccine adjuvant enhances protection against systemic methicillin-resistant (2009)
  • Author(s): Hu D-L, Narita K, Hyodo M, Hayakawa Y, Nakane A, Karaolis DKR
  • Journal Title: Staphylococcus aureus (MRSA) infection Volume: 27(35) Issue: 35 Pages: 4867-4873
  • DOI: 10.1016/j.vaccine.2009.04.053
  • Peer Reviewed
• [Journal Article] Immunization with a nontoxic mutant of staphylococcal enterotoxin A, SEA D227A, protects against enterotoxin-induced emesis in house musk shrews (2009)
  • Author(s): Hu D-L, Omoe K, Sashinami H, Shinagawa K, Nakane A
  • Journal Title: J Infect Dis Volume: 199(3) Issue: 3 Pages: 302-310
  • DOI: 10.1086/596065
  • Peer Reviewed
• [Journal Article] c-di-GMP as vaccine adjuvant enhances protection against systemic methicillin-resistant Staphylococcus aureus (MRSA) infection (2009)
  • Author(s): Hu D-L, Narita K, Hyodo M, Hayakawa Y, Nakane A, Karaolis DKRInfect Immun
  • Journal Title: Vaccine 27 Pages: 4867-4873
  • Peer Reviewed
• [Journal Article] Immunization with a nontoxic mutant of staphylococcal enterotoxin A, SEA D227A, protects against enterotoxin-inducedemesis in house musk shrews (2009)
  • Author(s): Hu D-L, Omoe K, Sashinami H, Shinagawa K, Nakane A.
  • Journal Title: J Infect Dis 199 Pages: 302-310
  • Peer Reviewed
• [Journal Article] c-di-GMP as vaccine adjuvant enhances protection against systemic methicillin-resistant Staphylococcus aureus(MRSA)infection (2009)
  • Author(s): Hu DL, Nakane A, et al.
  • Journal Title: Vaccine 27 Pages: 4867-4873
  • Peer Reviewed
• [Journal Article] Immunization with nontoxic mutant staphylococcal enterotoxin A, D227A, protects against enterotoxin-induced emesis in house musk shrews (2009)
  • Author(s): Hu DL, Nakane A, et.al.
  • Journal Title: J. Infect. Dis 199 Pages: 302-310
  • Peer Reviewed
• [Journal Article] Uchiyama T, Shinagawa K. Identification and characterization of two novel staphylococcal enterotoxins, types S and T (2008)
  • Author(s): Ono H, Omoe K, Imanishi K, Iwakabe Y, Hu D-L, Kato H, Saito N, Nakane A, Uchiyama T, Shinagawa K
  • Journal Title: Infect Immun Volume: 76(11) Issue: 11 Pages: 4999-5005
  • DOI: 10.1128/iai.00045-08
  • Peer Reviewed
• [Journal Article] Comparative prevalence of superantigenic toxin genes in methicillin-resistant and methicillin-susceptible Staphylococcus aureus islates (2008)
  • Author(s): Hu D-L, Omoe K, Inoue F, Kasai T, Yasujima M, Shinagawa K, Nakane A
  • Journal Title: J Med Microbiol Volume: 57(9) Issue: 9 Pages: 1106-1112
  • DOI: 10.1099/jmm.0.2008/002790-0
  • Peer Reviewed
• [Journal Article] Comparative prevalence of superantigenic toxin genes in methicillin-resistant and methicillin- susceptible Staphylococcus aureus isolates (2008)
  • Author(s): Hu D-L, Omoe K, Inoue F, Kasai T, Yasujima M, Shinagawa K, Nakane A.
  • Journal Title: J Med Microbiol 57(9) Pages: 1106-1112
  • Peer Reviewed
• [Journal Article] Comparative Prevalence of Superantigenic Toxin Genes in Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolates (2008)
  • Author(s): Hu DL, Nakane A., et.al.
  • Journal Title: J. Med. Microbiol 57 Pages: 1106-1112
  • Peer Reviewed
• [Journal Article] Identification and characterization of two novel staphylocoCcAl enteroloxins types S and T (2008)
  • Author(s): Ono H. Hu DL, Nakane A, et.al.
  • Journal Title: Infect. Immun 76 Pages: 4999-5005
  • Peer Reviewed
• [Presentation] Staphylococcal enterotoxin A is resistant to heating and digestive enzyme treatment on the emetic activity (2010)
  • Author(s): Hu D-L, Maina EK, Tomioka Y, Li SJ, Narita K, Osanai A, Shinagawa K, Omoe K, Nakane A
  • Organizer: The12^<th>Meeting of Hirosaki International Forum of medical Science
  • Place of Presentation: Hirosaki
  • Year and Date: 2010-10-29
• [Presentation] The role of IL-17A in the cell-mediated immunity induced by an immunization with clumping factor A against Staphylococcus aureus infection (2010)
  • Author(s): Narita K, Hu D-L, Nakane A
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-25
• [Presentation] The superantigenic activity of toxic shock syndrome toxin-1 is resistant to heating and digestive enzyme treatment (2010)
  • Author(s): Hu DL, Maina EK, Tomioka Y, Li SJ, Narita K, Osanai A, Shinagawa K, Omoe K, Nakane A
  • Organizer: 14^<th> International Congress of Immunolog
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-25
• [Presentation] Analysis of staphylococcal enterotoxin type A movement in gastrointestinal tract of house musk shrew and characterization of target cell of toxin (2010)
  • Author(s): Ono HK, Nishizawa M, Yamamoto Y, Hu DL, Nakane A, Omoe K
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-25
• [Presentation] The role of IL-17A in the cell-mediated immunity induced by an immunization with clumping factor A against Staphylococcus aureus infection (2010)
  • Author(s): Narita K, Hu D-L, Nakane A.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe International Exhibition Hall, Kobe, Japan
  • Year and Date: 2010-08-25
• [Presentation] The role of IL-17A in the cell-mediated immunity induced by animmunization with dumping factor A against Staphylococcus aureusinfection (2010)
  • Author(s): Kouji Narita
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸国際展示場(神戸市)
  • Year and Date: 2010-08-25
• [Presentation] c-di-GMP as a vaccine adjuvant enhances protection against Staphylococcus aureus infection (2010)
  • Author(s): Narita K, Hu D-L, Nakane A
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-29
• [Presentation] The role of interleukin-17 in the protective effect of an immunization with clamping factor A (2010)
  • Author(s): Hu D-L, Narita K, Nakane A
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-29
• [Presentation] c-di-GMP as a vaccine adjuvant enhances protection against Staphylococcus aureus infection (2010)
  • Author(s): Narita K, Hu D-L, Nakane A
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2010-03-29
• [Presentation] The role of interleukin-17 in the protective effect of an immunization with clamping factor A (2010)
  • Author(s): Hu D-L, Narita K, Nakane A
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2010-03-29
• [Presentation] C-di-GMP as a vaccine adjuvant enhances protection against Staphylococcus aureus infection (2010)
  • Author(s): 胡東良、成田浩司、中根明夫
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜パシフィコ(横浜市)
  • Year and Date: 2010-03-27
• [Presentation] 無毒変異体ブドウ球菌エンテロトキシンAの免疫による嘔吐予防効果 (2009)
  • Author(s): 胡東良、重茂克彦、差波拓志、品川邦汎、中根明夫
  • Organizer: 第56回トキシンシンポジウム
  • Place of Presentation: 長良川
  • Year and Date: 2009-08-26
• [Presentation] 無毒変異体ブドウ球菌エンテロトキシンAの免疫による嘔吐予防効果 (2009)
  • Author(s): 胡東良、重茂克彦、差波拓志、品川邦汎、中根明夫
  • Organizer: 第56回トキシンシンポジウム
  • Place of Presentation: 岐阜県長良川
  • Year and Date: 2009-08-26
• [Presentation] A nontoxic mutant staphylococcal enterotoxin A protects against enterotoxin-induced emesis (2009)
  • Author(s): 胡東良、重茂克彦、差波拓志、品川邦汎、中根明夫
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-12
• [Presentation] A nontoxic mutant staphylococcal enterotoxin A protects against enterotoxin-induced emesis (2009)
  • Author(s): 胡東良、重茂克彦、差波拓志、品川邦汎、中根明夫
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2009-03-12
• [Presentation] Vaccination with nontoxic mutant staphylococcal enterotoxin A, D227A, protects against enterotoxin-induced emesis in house musk shrews (2008)
  • Author(s): 胡東良、重茂克彦、差波拓志、品川邦汎、中根明夫
  • Organizer: 第31回日本分子生物学会・第81回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] クランピングファクターAによる免疫はIL-17産生を促進し黄色ブドウ球菌感染防御効果を示す (2008)
  • Author(s): 成田浩司、胡 東良、中根明夫
  • Organizer: 第38回日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] クランピングファクターAによる免疫はIL-17産生を促進し黄色ブトウ球菌感染防御効果を示す (2008)
  • Author(s): 成田浩司、胡東良、中根明夫
  • Organizer: 第38回日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] Staphylococcal enterotoxin A induces emesis through increasing serotonin release in intestine and endogenous cannabinoid system protects against the emetic response (2008)
  • Author(s): Hu, D-L, Omoe K, Shinagawa K, Nakane A
  • Organizer: 13th International Symposium on Staphylococci & Staphylococcal Infections
  • Place of Presentation: Carens, Australia
  • Year and Date: 2008-09-07
• [Presentation] Staphylococcal enterotoxin A induces emesis through increasing serotonin release in intestine and endogenous cannabinoid system protects against emetic response (2008)
  • Author(s): Hu D-L, Omoe K, Shinagawa K, Nakane A.
  • Organizer: 13^<th> International Symposium on Staphylococci and Staphylococcal Infections
  • Place of Presentation: Cairns Convention Centre, Cairns, Australia
  • Year and Date: 2008-09-07
• [Presentation] ブドウ球菌エンテロトキシンの嘔吐誘導とその制御機序 (2008)
  • Author(s): 胡 東良、重茂克彦、品川邦汎、中根明夫
  • Organizer: 第2回細菌学・若手コロッセウム
  • Place of Presentation: 湘南
  • Year and Date: 2008-08-03
• [Presentation] 黄色ブドウ球菌接着因子による感染予防効果 (2008)
  • Author(s): 成田浩司、胡 東良、重茂克彦、品川邦汎、中根明夫
  • Organizer: 第73回JSICR・第19回日本生体防御学会・第45回補体シンポジウム合同学会
  • Place of Presentation: 札幌
  • Year and Date: 2008-07-10
• [Presentation] 黄色フドワ球函接看因チによる感染予防効果 (2008)
  • Author(s): 成田浩司、胡東良、重茂克彦、品川邦汎、中根明夫
  • Organizer: 第73回JSICR・第19回日本生体防御学会・第45回補体シンポジウム合同学会
  • Place of Presentation: 札幌
  • Year and Date: 2008-07-10
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