Study on factors that determine the host specificity of Bordetella pertussis infection.

• Project/Area Number: 20390126
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka University
• Principal Investigator: HORIGUCHI Yasuhiko Osaka University, 微生物病研究所, 教授 (00183939)
• Co-Investigator(Kenkyū-buntansha): KAMITANI Shigeki 大阪大学, 微生物病研究所, 助教 (60379089) ; FUKUI Aya 大阪大学, 微生物病研究所, 助教 (70397743)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000); Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2009: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2008: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: 百日咳菌 / 気管支敗血症菌 / In vivo expression technology / DNAチップ / 秘管支敗血症菌 / IVET / マクロアレイ

Research Abstract

We developed a novel method termed IVET-IP (in vivo expressed-tag immunoprecipitation system), which enables us to identify bacterial genes expressed in host environments. This method could be useful to search factors that determine the host specificity of Bordetella pertussis.

Research Products

• [Journal Article] Bordetella dermonecrotic toxin binds to target cells via the N-terminal 30 amino acids. (2011)
  • Author(s): Fukui-Miyazaki, A., S.Ohnishi, S.Kamitani, H.Abe, Y.Horiguchi
  • Journal Title: Microbiol.Immunol. 55 Pages: 154-159
  • NAID: 10031121454
  • Peer Reviewed
• [Journal Article] Bordetella dermonecrotic toxin binds to target cells via the N-terminal 30 amino acids. (2011)
  • Author(s): Fukui-Miyazaki A., Horiguchi Y., et al.
  • Journal Title: Microbiology and Immunology Volume: 55 Pages: 154-159
  • NAID: 10031121454
  • Peer Reviewed
• [Journal Article] Clostridium perfringens enterotoxin interacts with claudins via electrostatic attraction. (2010)
  • Author(s): Kimura, J., H.Abe, S.Kamitani, H.Toshima, A.Fukui, Miyake, M.Y.Kamata, Y.Sugita-Konishi, S.Yamamoto, Y.Horiguchi
  • Journal Title: The Journal of biological chemistry 285 Pages: 401-408
  • Peer Reviewed
• [Journal Article] Characterization of the membrane-targeting C1 domain in Pasteurella multocida toxin. (2010)
  • Author(s): Kamitani, S., K.Kitadokoro,, M.Miyazawa, H.Toshima, A.Fukui, H.Abe, M.Miyake, Y.Horiguchi
  • Journal Title: The Journal of biological chemistry 285 Pages: 25467-25475
  • Peer Reviewed
• [Journal Article] Association of Bordetella dermonecrotic toxin with the extracellular matrix. (2010)
  • Author(s): Fukui-Miyazaki, A., S.Kamitani, M.Miyake, Y.Horiguchi
  • Journal Title: BMC microbiology 10 Pages: 247-247
  • Peer Reviewed
• [Journal Article] Characterization of the membrane-targeting C1 domain in Pasteurella multocida toxin. (2010)
  • Author(s): Kamitani, S., Horiguchi Y., et al.
  • Journal Title: The Journal of Biological Chemistry Volume: 285(33) Pages: 25467-25475
  • Peer Reviewed
• [Journal Article] Association of Bordetella dermonecrotic toxin with the extracellular matrix. (2010)
  • Author(s): Fukui-Miyazaki, A., Horiguchi Y., et al.
  • Journal Title: BMC Microbiology Volume: 10 Pages: 247-255
  • Peer Reviewed
• [Journal Article] Clostridium perfringens enterotoxin interacts with claudins via electrostatic attraction (2010)
  • Author(s): Kimura, J., et al
  • Journal Title: J Biol Chem 285 Pages: 401-408
  • Peer Reviewed
• [Journal Article] A novel tumor-targeted therapy using a claudin-4-targeting molecule. (2009)
  • Author(s): Saeki, R., M.Kondoh, H.Kakutani, S.Tsunoda, Y.Mochizuki, T.Hamakubo, Y.Tsutsumi, Y.Horiguchi, K.Yagi
  • Journal Title: Mol Pharmacol 76 Pages: 918-926
  • Peer Reviewed
• [Journal Article] Human herpesvirus 6 ganciclovir-resistant strain with amino acid substitutions associated with the death of an allogeneic stem cell transplant recipient. (2009)
  • Author(s): Isegawa, Y., J.Hara, K.Amo, Y.Osugi, M.Takemoto, K.Yamanishi, R.Fukunaga, M.Shibata,, A.Ohshima, Y.Horiguchi, N.Sugimoto
  • Journal Title: J Clin Virol 44 Pages: 15-19
  • Peer Reviewed
• [Journal Article] A novel tumor-targeted therapy using a claudin-4-targeting molecule (2009)
  • Author(s): Saeki, R., et al
  • Journal Title: Mol Pharmacol 76(4) Pages: 918-926
  • Peer Reviewed
• [Journal Article] Human herpesvirus 6 ganciclovir-resistant strain with amino acid substitutions associated with the death of an allogeneic stem cell transplant recipient (2009)
  • Author(s): Isegawa, Y., et al
  • Journal Title: J Clin Virol 44(1) Pages: 15-19
  • Peer Reviewed
• [Journal Article] Domain mapping of a claudin-4 modulator, the C-terminal region of C-terminal fragment of Clostridium perfringens enterotoxin, by site-directed mutagenesis. (2008)
  • Author(s): Takahashi, A., E.Komiya, H.Kakutani, T.Yoshida, M.Fujii, Y.Horiguchi, H.Mizuguchi, Y.Tsutsumi, S.Tsunoda, N.Koizumi, K.Isoda, K.Yagi, Y.Watanabe, M.Kondoh
  • Journal Title: Biochem Pharmacol 75 Pages: 1639-1648
  • Peer Reviewed
• [Journal Article] The morphological changes in cultured cells caused by Bordetella pertussis adenylate cyclase toxin. (2008)
  • Author(s): Ohnishi, H., M.Miyake, S.Kamitani, Y.Horiguchi
  • Journal Title: FEMS Microbiol Lett 279 Pages: 174-179
  • Peer Reviewed
• [Journal Article] A colorimetric assay for studying effector secretion through the bacterial type III secretion system. (2008)
  • Author(s): Miyake, M., S.Sakane, C.Kobayashi, M.Hanajima-Ozawa, A.Fukui, S.Kamitani, Y.Horiguchi
  • Journal Title: FEMS Microbiol Lett 278 Pages: 36-42
  • Peer Reviewed
• [Journal Article] Characterization of the human herpesvirus 6 U69 gene product and identification of its nuclear localization signal. (2008)
  • Author(s): Isegawa, Y., Y.Miyamoto, Y.Yasuda, K.Semi, K.Tsujimura, R.Fukunaga, A.Ohshima, Y.Horiguchi, Y.Yoneda, N.Sugimoto
  • Journal Title: J Virol 82 Pages: 710-718
  • Peer Reviewed
• [Journal Article] Crystal structures reveal a thiol protease-like catalytic triad in the C-terminal region of Pasteurella multocida toxin. (2007)
  • Author(s): Kitadokoro, K., S.Kamitani, M.Miyazawa, M.Hanajima-Ozawa, A.Fukui, M.Miyake, Y.Horiguchi
  • Journal Title: Proc Natl Acad Sci USA 104 Pages: 5139-5144
  • Peer Reviewed
• [Journal Article] Role of tyrosine residues in modulation of claudin-4 by the C-terminal fragment of Clostridium perfringens enterotoxin (2007)
  • Author(s): Harada, M., M.Kondoh, C.Ebihara, A.Takahashi, E.Komiya, M.Fujii, H.Mizuguchi, S.Tsunoda, Y.Horiguchi, K.Yagi, Y.Watanabe
  • Journal Title: Biochem Pharmacol 73 Pages: 206-214
  • Peer Reviewed
• [Journal Article] Enteropathogenic Escherichia coli, Shigella flexneri, and Listeria monocytogenes recruit a junctional protein, zonula occludens-1, to actin tails and pedestals. (2007)
  • Author(s): Hanajima-Ozawa, M., T.Matsuzawa, A.Fukui, S.Kamitani, H.Ohnishi, A.Abe, Y.Horiguchi, M.Miyake
  • Journal Title: Infect Immun 75 Pages: 565-573
  • Peer Reviewed
• [Journal Article] Role of Tyr306 in the C-terminal fragment of Clostridium perfringens enterotoxin for modulation of tight junction. (2007)
  • Author(s): Ebihara, C., M.Kondoh, M.Harada, M.Fujii, H.Mizuguchi, S.Tsunoda, Y.Horiguchi, K.Yagi, Y.Watanabe
  • Journal Title: Biochem Pharmacol 73 Pages: 824-830
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary crystallographic studies of the Pasteurella multocida toxin catalytic domain. (2006)
  • Author(s): Miyazawa, M., K.Kitadokoro, S.Kamitani, H.Shime, Y.Horiguchi
  • Journal Title: Acta Crystallograph Sect F Struct Biol Cryst Commun 62 Pages: 906-908
  • Peer Reviewed
• [Journal Article] Dermonecrotic toxin: The old but new virulence factor produced by Bordetella spp. (2006)
  • Author(s): Fukui, A., Y.Horiguchi
  • Journal Title: Toxin Rev. 25 Pages: 125-135
  • Peer Reviewed
• [Journal Article] Preparation of a claudin-targeting molecule using a C-terminal fragment of Clostridium perfringens enterotoxin. (2006)
  • Author(s): Ebihara, C., M.Kondoh, N.Hasuike, M.Harada, H.Mizuguchi, Y.Horiguchi, M.Fujii, Y.Watanabe
  • Journal Title: J Pharmacol Exp Ther 316 Pages: 255-260
  • Peer Reviewed
• [Presentation] ウェルシュ菌エンテロトキシンの受容体認識機構の解析 (2009)
  • Author(s): 木村淳, 安倍裕順, 神谷重樹, 戸嶋ひろ野, 福井理, 三宅眞実, 堀口安彦
  • Organizer: 日本細菌学会
  • Place of Presentation: 名古屋市名古屋国際会議場
  • Year and Date: 2009-03-12
• [Presentation] ウェルシュ菌エンテロトキシンの受容体認識機構の解析 (2008)
  • Author(s): 木村淳, 安倍裕順, 神谷重樹, 戸嶋ひろ野, 福井理, 三宅眞実, 堀口安彦
  • Organizer: 分子生物学会
  • Place of Presentation: 横浜市パシフィコ横浜
  • Year and Date: 2008-12-09
• [Presentation] パスツレラ毒素C1ドメインの膜局在化能の機能解析 (2008)
  • Author(s): 神谷重樹, 北所健悟, 戸嶋ひろ野, 福井理, 三宅眞実, 堀口安彦
  • Organizer: 日本細菌学会
  • Place of Presentation: 京都市国立京都国際会館
  • Year and Date: 2008-03-24
• [Presentation] Bordetella皮膚壊死毒素の細胞外マトリクスへの付着 (2007)
  • Author(s): 福井理, 神谷重樹, 三宅眞実, 堀口安彦
  • Organizer: 日本分子生物学会日本生化学会合同大会
  • Place of Presentation: 横浜市パシフィコ横浜
  • Year and Date: 2007-12-11
• [Presentation] ウエルシュ菌エンテロトキシンの受容体認識機構の解析 (2007)
  • Author(s): 木村淳, 神谷重樹, 戸嶋ひろ野, 福井理, 三宅眞実, 堀口安彦
  • Organizer: 日本分子生物学会日本生化学会合同大会
  • Place of Presentation: 横浜市パシフィコ横浜
  • Year and Date: 2007-12-11
• [Presentation] Pasteurellamultocida toxinに存在するシステインプロテアーゼ様活性中心 (2007)
  • Author(s): 神谷重樹, 北所健悟, 宮澤雅之, 福井理, 三宅眞実, 堀口安彦
  • Organizer: 日本細菌学会
  • Place of Presentation: 大阪市アジア太平洋トレードセンター
  • Year and Date: 2007-03-26
• [Presentation] Pasteurellamultocida toxin(PMT)の活性ドメインの全体構造 (2007)
  • Author(s): 北所健悟, 神谷重樹, 宮澤雅之, 福井理, 三宅眞実, 堀口安彦
  • Organizer: 日本細菌学会
  • Place of Presentation: 大阪市アジア太平洋トレードセンター
  • Year and Date: 2007-03-26
• [Presentation] Bordetella属細菌の菌体遊離型壊死毒の検出 (2006)
  • Author(s): 木村淳, 福井理, 神谷重樹, 三宅眞実, 堀口安彦
  • Organizer: 日本細菌学会
  • Place of Presentation: 金沢市観光会館
  • Year and Date: 2006-03-29
• [Presentation] 腸管病原性大腸菌3型分泌装置により分泌されるエフェクターの宿主細胞内移行検出系の開発 (2006)
  • Author(s): 坂根貞嗣, 花嶋美幸, 堀口安彦, 三宅眞実
  • Organizer: 日本細菌学会
  • Place of Presentation: 金沢市観光会館
  • Year and Date: 2006-03-29
• [Remarks] ホームページ等
  • URL: http://bactox1.biken.osaka-u.ac.jp