Metagenomic diagnosis of bacterial infections

• Project/Area Number: 20390128
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka University
• Principal Investigator: IIDA Tetsuya Osaka University, 微生物病研究所, 特任教授 (90221746)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2009: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2008: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
• Keywords: 次世代シーケンサ / 細菌感染症 / 迅速診断法 / 検出 / 下痢症 / 診断 / メタゲノム

Research Abstract

We have been applying so-called "high-throughput DNA sequencers" to the analysis of infectious diseases, especially enteric bacterial infections. The approach is in principle a method that does not depend on the type of target pathogens, so that it is possible to analyze various microorganisms with a single common protocol. The approach enables us to conduct an unbiased analysis of dynamics of the pathogen and associated microbiota in human specimens during the course of infectious diseases.

Research Products

• [Journal Article] Metagenomic analysis of bacterial infections by means of high-throughput DNA sequencing. (2011)
  • Author(s): Nakamura S., Nakaya T., Iida T.
  • Journal Title: Exp.Biol.Med. in press
  • Peer Reviewed
• [Journal Article] Metagenomic analysis of bacterial infections by means of high-throughput DNA sequencing (2011)
  • Author(s): Nakamura, S., Iida, T., et al.
  • Journal Title: Exp.Biol.Med. Volume: (未定 印刷中)
  • Peer Reviewed
• [Journal Article] 次世代シーケンサを用いた細菌感染症のメタゲノミック診断 (2010)
  • Author(s): 飯田哲也
  • Journal Title: Medical Technology 38 Pages: 227-229
• [Journal Article] 次世代シーケンサを用いた細菌感染症のメタゲノミック診断 (2010)
  • Author(s): 飯田哲也
  • Journal Title: Medical Technology Volume: 38 Pages: 227-229
• [Journal Article] Two regulators of Vibrio parahaemolyticus play important roles in enterotoxicity by controlling the expression of genes in the Vp-PAI region. (2010)
  • Author(s): Kodama, et al.
  • Journal Title: PLoS One 5
  • Peer Reviewed
• [Journal Article] Association of Vibrio parahaemolyticus thermostable direct hemolysin with lipid rafts is essential for its exerting cytotoxic effect but not its he molvtic activity. (2010)
  • Author(s): Matsuda, et al.
  • Journal Title: Infect.Immun. 78 Pages: 603-610
  • Peer Reviewed
• [Journal Article] Direct Metagenomic Detection of Viral Pathogens in Nasal and Fecal Specimens using an Unbiased High-throughput Sequencing Approach. (2009)
  • Author(s): Nakamura S., Yang C.-S., Sakon N., Ueda M., Tougan T., Yamashita A., Goto N., Takahashi K., Yasunaga T., Ikuta K., Mizutani T., Okamoto Y., Tagami M., Morita R., Maeda N., Kawai J., Hayashizaki Y., Nagai Y., Horii T., Iida T., Nakaya T.
  • Journal Title: PLoS One 4
  • Peer Reviewed
• [Journal Article] 次世代シーケンサを用いた細菌感染症のメタゲノミック診断 (2009)
  • Author(s): 飯田哲也
  • Journal Title: JVM 62 Pages: 819-820
• [Journal Article] 次世代シーケンサーを用いた微生物・感染症解析 (2009)
  • Author(s): 飯田哲也
  • Journal Title: 医学のあゆみ 231 Pages: 180-181
  • NAID: 10029759115
• [Journal Article] Genomic taxonomy of vibrios. (2009)
  • Author(s): Thompson, et al.
  • Journal Title: BMC Evol.Biol. 9 Pages: 258-258
  • Peer Reviewed
• [Journal Article] Direct metagenomic detect ion of viral pathogens in nasal and fecal specimens using an unbiased high-throughput seauencing approach (2009)
  • Author(s): Nakamura, et al.
  • Journal Title: PLoS One 4
  • Peer Reviewed
• [Journal Article] Metagenomic diagnosis of bacterial infections. (2008)
  • Author(s): Nakamura, S., Maeda, N., Miron, I.M., Yoh, M., Izutsu, K., Kataoka, C., Honda, T., Yasunaga, T., Nakaya, T., Kawai, J., Hayashizaki, Y., Horii, T., Iida T.
  • Journal Title: Emerg.Infect.Dis. 14 Pages: 1784-1786
  • Peer Reviewed
• [Journal Article] Metagenomic diagnosis of bacterial infections (2008)
  • Author(s): Nakamura, et al.
  • Journal Title: Emerg. Infect. Dis. 14 Pages: 1784-1786
  • Peer Reviewed
• [Journal Article] Differential replication dynamics for large and smal1 Vibriochromosomes affect gene dosage, expression and location. (2008)
  • Author(s): Dryselius, et al.
  • Journal Title: BMC Genomics 9 Pages: 559-559
  • Peer Reviewed
• [Presentation] 次世代DNAシーケンサの細菌感染症への応用 (2010)
  • Author(s): 飯田哲也
  • Organizer: 特別講演日本細菌学会中国四国支部総会
  • Place of Presentation: 松山大学
  • Year and Date: 2010-10-19
• [Presentation] 次世代DNAシーケンサの細菌感染症への応用 (2010)
  • Author(s): 飯田哲也
  • Organizer: 日本細菌学会中国四国支部総会
  • Place of Presentation: 松山大学(招待講演)
  • Year and Date: 2010-10-16
• [Presentation] Metagenomic analysis of infectious diseases. (2010)
  • Author(s): 飯田哲也
  • Organizer: The 10th Japan-Korea International Symposium on Microbiology
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 2010-03-26
• [Presentation] Metagenomic analysis of enteric infections. (2010)
  • Author(s): 飯田哲也
  • Organizer: 14^<th> International Conference on Emerging Infectious Diseases in the Pacific Rim : Next Generation Diagnostics for Infectious Diseases.
  • Place of Presentation: Penang, Malaysia
• [Presentation] I believe in sequencing. Symposium : Future high-throughput microbiology laboratory. (2010)
  • Author(s): 飯田哲也
  • Organizer: 20th ECCMID, European Cngress of Clinical Microbiology and Infectious Diseases
  • Place of Presentation: Vienna, Austria
• [Presentation] Metagenomic analysis of intestinal microbiota in infections.国際シンポジウムGut defense and microbial infections (2010)
  • Author(s): 飯田哲也
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Metagenomic diagnosis of infectious diseases. (2009)
  • Author(s): Tetsuya Iida
  • Organizer: 12^<th> ISTC-SAC Seminar -Combatine Global Infections-
  • Place of Presentation: Irkutsk, Russia
  • Year and Date: 2009-09-22
• [Presentation] 次世代シーケンサを用いた感染症のメタゲノミツク診断 (2009)
  • Author(s): 飯田哲也
  • Organizer: 第3回日本ゲノム微生物学会
  • Place of Presentation: 中央大学理工学部
  • Year and Date: 2009-03-07
• [Presentation] 次世代シーケンサを用いた微生物・感染症解析 (2009)
  • Author(s): 飯田哲也
  • Organizer: 特別講演.日本微生物資源学会第16回大会
  • Place of Presentation: 大阪大学銀杏会館
• [Presentation] Metagenomic diagnosis of infectious diseases. (2009)
  • Author(s): 飯田哲也
  • Organizer: 12^<th> ISTC-SAC Seminar. Combating Global Infections-
  • Place of Presentation: Irkutsk, Russia
• [Presentation] Metagenomic approach for detection of bacterial pathogens. (2008)
  • Author(s): 飯田哲也
  • Organizer: 2008 International Symposium & Annual Meeting of the Korean Society for Microbiology and Biotechnology.
  • Place of Presentation: Seoul KyoYuk MunHwa HoeKwan, Seoul, Korea
• [Book] Direct Metagenomic Detection of Viral Pathogens in Human Specimens using an Unbiased High-throughput Sequencing Approach. In: Handbook of Molecular Microbial Ecology II: Metagenomics in Different Habitats(Ed. Frans J. de Bruijn. Wiley-Blackwell)Hoboken, NJ, USA. (2011)
  • Author(s): Nakaya T., Nakamura S., Okamoto Y., Nagai Y., Kawai J., Hayashizaki Y., Iida T., Horii, T.
  • Publisher: an imprint of John Wiley & Sons Ltd in press
• [Book] Handbook of Molecular Microbial Ecology II : Metagenomics in Different Habitats (2011)
  • Author(s): Nakaya, T., Iida, T., et al.
  • Publisher: Wiles-Blackwell(印刷中)
• [Book] 次世代シークエンサーを用いた感染症の診断と解析メタゲノム解析技術の最前線 (2010)
  • Author(s): 中村昇太、中屋隆明、飯田哲也
  • Publisher: シーエムシー出版
• [Book] メタゲノム解析技術の最前線 (2010)
  • Author(s): 中村昇太、飯田哲也、, 他
  • Publisher: シーエムシー出版