Pathologic application of atomic analyzed SEM and infrared microscopic imaging in tumor tissue.

• Project/Area Number: 20390165
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: University of Fukui
• Principal Investigator: MIYOSHI Norio University of Fukui, 医学部, 助教 (40209961)
• Co-Investigator(Kenkyū-buntansha): IMAMURA Yoshiaki 福井大学, 医学部附属病院, 准教授 (40223341)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2010: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2009: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000); Fiscal Year 2008: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
• Keywords: 臨床検査システム / 赤外分光病態電顕検査システム / タンパク質2次構造成分 / 赤外分光がんマーカー / FT-IR顕微分光画像診断 / TNNI2関連蛋白 / α-ヘリックス構造成分比率

Research Abstract

Pathologic application study of and atomic analyzed scanning electron microscopic (SEM) and an infrared microscopic imaging in tumor tissue was done using SEN attached with an energy dispersion X-ray analyzer and FT-IR microscope.

Research Products

• [Journal Article] Targetig of sebaceous glands by delta-aminolevulinic acid-based photodynamic therapy : an in vivo study. (2011)
  • Author(s): Kosak, S., Miyoshi, N., Akilov, O.R., Hasan, T., Kawana, S.
  • Journal Title: photochem. & photobiol. Volume: (Accepted)
  • Peer Reviewed
• [Journal Article] 展望「癌の凍結切片組織のFT-IR顕微鏡観察による新バイオイメージング法の開発」 (2011)
  • Author(s): 三好憲雄
  • Journal Title: 日本化学会「ぶんせき」 Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] 総説「癌凍結切片組織のFT-IRイメージング」 (2011)
  • Author(s): 三好憲雄
  • Journal Title: 日本赤外線学会・特集号「ライフサイエンスと赤外線(Part-2)」 Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Targeting of sebaceous glands by delta-aminolevulinic acid-based photodynamic therapy : an in vivo study. (2010)
  • Author(s): S.Kosaka, N.Miyoshi^*, O.R.Akilov, T.Hasan.
  • Journal Title: Lasers in Surg.& Med.(*Corresponding Author)
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): S.Ito, N.Miyoshi^*, V.DeGraff, K.Nagashima, L.Kirschnbaum, P.Riesz.
  • Journal Title: Free Radical Res.(*Corresponding Author) (12) Pages: 1214-1224
  • Peer Reviewed
• [Journal Article] Terahetz pulsed imaging of frozen biological tissues (2009)
  • Author(s): Hoshina, H, Hayashi, A, Miyoshi, N, Miyamaru, F, Otani, C.
  • Journal Title: Applied Physics Letters 94 Pages: 123901-3
  • Peer Reviewed
• [Journal Article] Enhancement of 5-aminolevulinic acid-onduced oxidative stress on two cancer cell lines by gold nanoparticeles (2009)
  • Author(s): Ito, S, Miyoshi N., DeGraff, WG, Nagashima, K, Kischenbaum, LJ, Riesz, P.
  • Journal Title: Free Radical Ressearch 43(12) Pages: 1214-1224
  • Peer Reviewed
• [Journal Article] Phosphate mapping of sialoliths with raman microspectroscopy. (2008)
  • Author(s): S.Kioshita, N.Miyoshi^*, K.Miyauchi, T.Ogawa, T.Kitagawa, H.Itoh, K.Sano.
  • Journal Title: J.Rama Spectrosc.(*Corresponding Author) 39 Pages: 349-353
  • Peer Reviewed
• [Journal Article] Ultrasound induced cytolysis of cancer cells is enhanced in the presence of micron-sized slminum particles. (2008)
  • Author(s): N.Miyoshi^*, T.Tuziuti, K.Yasui, Y.lida, J.Z.Sostaric.
  • Journal Title: Ultrasonics Sonochemistry(*Corresponding Author) 15(5) Pages: 831-890
  • Peer Reviewed
• [Journal Article] Functional mapping of caries enamel of a human teeth with Raman microspectroscopy. (2008)
  • Author(s): H.Kinoshita, N.Miyoshi^*, T.Ogasawara, K.Sano.
  • Journal Title: J.Raman Spectrosc.(*Corresponding Author) 39 Pages: 655-660
  • Peer Reviewed
• [Journal Article] Characterization of chitosan/citrate and chitosan/acetate films and applications for wound healing. (2008)
  • Author(s): J.Tanigawa, N.Miyoshi^*, K.Sakurai.
  • Journal Title: J.Appl.Polym.Sci.(*Corresponding Author) 100 Pages: 608-615
  • NAID: 120001493898
  • Peer Reviewed
• [Journal Article] Dynamic adsorption properties of n-alkylglucopyranosides determine their ability to inhibit cytolysismediated by acoustic cavitations. (2008)
  • Author(s): J.Z.Sostaric, N.Miyoshi, J.Y.Cheng, P.Riesz.
  • Journal Title: J.Phys.Chem, B. 112(40) Pages: 12703-12709
  • Peer Reviewed
• [Journal Article] Terahertz pulsed imaging of frozen histological tissues. (2008)
  • Author(s): H.Hoshina, A.Hayashi, N.Mivoshi, F.Miyauchi, C.Otani.
  • Journal Title: Appl.Phys.Lett. 94(12)
  • Peer Reviewed
• [Journal Article] Dynamic absorption properties of n-alkyl glucopyranosides determine their ability to inhibit cytolysis mediated by acoustic cavitation. (2008)
  • Author(s): Sostaric, Z. J., Miyoshi, N, Cheng, Y. J., Riesz, P.
  • Journal Title: J. Phys. Chem. B 112(40) Pages: 12703-12709
  • Peer Reviewed
• [Journal Article] Functional maping of carious enamel in human teeth with Raman microspectros copy. (2008)
  • Author(s): Kinoshita, H., Miyoshi. N., Fukunaga, Y., Ogawa, T., Ogasawara, T., Sano, K.
  • Journal Title: J. Raman Spectroscopy 39 Pages: 655-660
  • Peer Reviewed
• [Journal Article] Ultrasound-induced cytolysis of cancer cells is enhanced in the presence of micron sized alumina particles. (2008)
  • Author(s): Mivoshi, N., Tuziuti, T., Yasui, K., Iida, Y., Riesz, P., Sostaric, Z. J.
  • Journal Title: Ultrasonics Sonochemistry 15(6) Pages: 881-890
  • Peer Reviewed
• [Journal Article] Fluorescence-guided ressection of met astatic brain tumors using a 5-amino-levulinic acid-induced protoporphyrin-IX : photobiological study. (2007)
  • Author(s): S.Utsuki, N.Miyoshi^*, H.Oka, H.Misajima, S.Shimizu, S.Suzuki, K.Fuji.
  • Journal Title: Brain Tumor Pathol.(*Corresponding Author) 23 Pages: 53-55
  • Peer Reviewed
• [Journal Article] PD and PDT with porphyrin precursors in Japan. (2007)
  • Author(s): N.Miyoshi^*, S.Kaneko.
  • Journal Title: J Env Pathology, Toxicology and Oncology(*Corresponding Author) 27 Pages: 108-113
  • Peer Reviewed
• [Presentation] In hope of getting over the present stage of clinical field. (2010)
  • Author(s): N.Miyoshi^*
  • Organizer: in PACIFICHEM 2010.Symposium of PD and PDT (#12-071)(*Corres-ponding Author)
  • Place of Presentation: Hawaii.(Symposium-3E : (Corres-ponding Organizer, Chair))
  • Year and Date: 2010-12-18
• [Presentation] Combination treatment of hyperthermia and PDT for experimental tumor model using gyrotron(107,203GHz). (2010)
  • Author(s): Miyoshi, N.Ito, S., Ogawa, I., Idehare, T.
  • Organizer: IRMMW-THz, 2010,35th Int. Conference on Infrared, Millimeter and Terahertz wave
  • Place of Presentation: Roma, Italy
  • Year and Date: 2010-10-25
• [Presentation] Symposium, Present and future of photodvnamic laser therapv. (2010)
  • Author(s): Miyoshi, N.
  • Organizer: The Asian Pucific Association for Laser Med. and Sur.
  • Place of Presentation: Suwa, Nagano
  • Year and Date: 2010-10-18
• [Presentation] Combination treatment of hyperthermia and PDT for experimental tumor model using gyrotron (107, 203 GHz). (2010)
  • Author(s): N.Miyoshi^*, S.Ito, I.Ogawa, T.Idehara.
  • Organizer: In 35th Int.Conference on Infrared, Millimeter and Terahertz Wave (IRMMW-THz 2010)(*Corresponding Author)
  • Place of Presentation: Roma.(Symposium)
  • Year and Date: 2010-09-14
• [Presentation] Introduction : In hope of detting over the present stage of clinical field. (2010)
  • Author(s): N.Miyoshi^* K.Inoue, T.Tanaka.
  • Organizer: The 2nd Asia Japan-Korea-China PDT Joint Symposium(*Corresponding Author)
  • Place of Presentation: Fukui.(Symposium, Corresponding Organizer, Chair)
  • Year and Date: 2010-06-11
• [Presentation] Introduction for Symposium"In hope of getting over the presentstepofclinicalfield". (2010)
  • Author(s): Miyoshi, N. Inoue, K., Tanaka, T.
  • Organizer: The 2nd Asia Japan-Korea-ChinaPDT Joint SympOSlum
  • Place of Presentation: Fukui-ity, AOSSA-8F
  • Year and Date: 2010-06-11
• [Presentation] In hope of getting over the present step of clinical field. (2010)
  • Author(s): Miyoshi, N.
  • Organizer: PACIFICHEM2010, Symposium(#12-071)
  • Place of Presentation: Hawaii Convention Center
• [Presentation] PDD/PDT光源の開発と狂く手新しい光増感剤の開発 (2010)
  • Author(s): 三好憲雄、井上克司、田中徹
  • Organizer: 第20回日本光線力学学会学術講演会
  • Place of Presentation: 福井市・県民ホール
• [Presentation] Application for hyperthermia treatment of an experimental tumor using a gyrotron (107, 203 GHz). (2009)
  • Author(s): N.Miyoshi^*, Y.Fukunaga, I.Ogawa, T.Idehara.
  • Organizer: The 34th Int.Conference on Infrared, Milimeter and Teraherrz Waves (IRMMW-THz, 2009)(*Corresponding Author)
  • Place of Presentation: Korea(Symposium Session)
  • Year and Date: 2009-11-23
• [Presentation] Combination therapy of PDT with proton irradiation. (2009)
  • Author(s): N.Miyoshi^*, Y.Fukunaga, K.Kume, E.Minehara.
  • Organizer: 18th Congress of the Int.Soc.for Laser Surg.Med.(*Corresponding Author)
  • Place of Presentation: Tokyo.(Symposium)
  • Year and Date: 2009-11-12
• [Presentation] Introduction of Japanese 5-ALA Association on Basic and Clinical Studies.(Abstract Book, p.19) (2009)
  • Author(s): N.Miyoshi^*, S.Kaneko, Y.Fukunaga, R.Kitai, M.Ishizuka, Y.Matsumoto, S.Kosaka, K.Inoue, T.Akasu, T.Tanaka, H.Hisazumi.
  • Organizer: 9th Annual Congress the Korean Photodynamic Association and 1st Korean-Japan Photodynamic Symposium(*Corresponding Author)
  • Place of Presentation: Korea.(Plenary Lecture (Invited))
  • Year and Date: 2009-08-22
• [Presentation] Introduction of Japanese 5-ALA Association on Basic and Clinical Studies (2009)
  • Author(s): Miyoshi, N., Kaneko, S, Fukunaga, Y., Kitai, S, Ishizuka M, Matsumoto, Y, Kosaka S, Inoue, K, Akasu, T, Tanaka, T, Hisazumi, H.
  • Organizer: The 9^<th> Annual Congress the Korean Photodynamic Association and the 4th International & 1st Asia Korea-Japan Photodynamic Therapy Symposium
  • Place of Presentation: The Catholic University of Korea, Seoul city, Korea
• [Presentation] Modification of porphyrin precursor, 5-ALA with TiO_2 particles and the photodynamic effect for experimental tumor model.(Abstract book, p.18) (2008)
  • Author(s): N.Miyoshi^*, Y.Fukunaga, K.Sugimoto, H.Hisazumi.
  • Organizer: The 12th Congress of Asian Pacific Association for Laser Medicine and Surgery.(*Corresponding Author)
  • Place of Presentation: Korea.(Invited Speaker)
  • Year and Date: 2008-10-18
• [Presentation] Modification of porphyrin precursor 5-ALA with TiO_2 particles and the photodynamic effect for experimental tumor model. (2008)
  • Author(s): Mivoshi. N., Fukunaga, Y., Sugimoto, K., Hisazumi, H.
  • Organizer: The 12^<th> Congress of Asian Pacific Association for Laser Medicine & Surgery.
  • Place of Presentation: Yonsei University, Seoul city, Korea
• [Presentation] 美容1:皮膚科における光線力学療法(PDT)の基礎研究 (2007)
  • Author(s): 三好憲雄^*
  • Organizer: 第25回日本美容皮膚科学会総会・学術大会
  • Place of Presentation: 横浜(教育講演)
  • Year and Date: 2007-08-18
• [Presentation] サテライトシンポジウム:5-ALA の基礎研究と臨床研究の利用拡大を目指して (2007)
  • Author(s): 三好憲雄^*
  • Organizer: 第17回日本光線力学学会学術講演会
  • Place of Presentation: 栃木
  • Year and Date: 2007-06-16
• [Presentation] Modification of porphyrin precursor, 5-ALA with TiO_2 particles by ultrasound irradiation. (2007)
  • Author(s): N.Miyoshi^*, T.Ogasawara, H.Kinoshita, H.Hisazumi.
  • Organizer: The 11th World Congress of the Int.Photodynamic Association (IPA)(*Corresponding Author)
  • Place of Presentation: Seattle.(Workshope)
  • Year and Date: 2007-05-29
• [Book] ナノ材料のリスク評価と安全性対策(6章:酸化チタンとナノリスク「ナノ粒子がもたらす有効性と環境保護への心配から」)(フロンティアテクノシリーズ「)(*Corresponding Author) (2010)
  • Author(s): 三好憲雄*、伊藤慎治、福永幸裕
  • Publisher: フロンティア出版、東京
• [Book] エレクトロニクス「次世代光医療-レーザー技術の臨床への橋渡しー」(第12章:「利用拡大を目指した5-ALAを用いたがんのPDD/PDT 」)(*Corresponding Author) (2010)
  • Author(s): 三好憲雄*、伊藤慎治、福永幸裕
  • Publisher: シーエムシー出版
• [Book] フロンティアテクノシリーズ「ナノ材料のリスク評価と安全性対策」監修:直理文夫;フロンティア出版 (2010)
  • Author(s): 三好憲雄脚、伊藤慎治、福永幸裕
  • Total Pages: 317
  • Publisher: 第6章;酸化チタンとナノリスク「ナノ粒子がもたちす有効性と環境保健への心配から」
• [Book] エレクトロニクスシリーズ「次世代光医療:レーザー技術の臨床への橋渡し」監修:粟津邦雄;シーエムシー出版 (2010)
  • Author(s): 三好憲雄、伊藤慎治、福永幸裕
  • Total Pages: 252
  • Publisher: 第12章:「利用拡大を目指した5-ALAを用いたがんのPDD/PDT」
• [Book] 「次世代光医療-光技術の臨床への橋渡し」第13章 利用拡大を目指した5-ALAを用いたがんのPDD-PDT (2010)
  • Author(s): 三好憲雄, 伊藤慎治, 福永幸裕, 木下英荘
  • Publisher: (株) シーエムシー出版
• [Book] An application of fluorescence analysis in tumor tissue.(Eds.by 三好憲雄, S.Kaneko ; pp.1-92)(Recent Progress on Clinical and Basic Research of ALA.)(*Corresponding Author) (2007)
  • Author(s): N.Miyoshi^*, S.Kaneko, H.Hisazumi.
  • Publisher: Published by Kashiwaba Neurosurgical Hospital.
• [Book] Distribution of protoporphyrin-IX (Pp-IX) in the animal acne model.(Eds.by 三好憲雄, S.Kaneko; pp.1-92)(Recent Progress on Clinical and Basic Research of ALA.)(*Corresponding Author) (2007)
  • Author(s): K.Nakano, E.Tachihara, N.Miyoshi^*.
  • Publisher: Published by Kashiwaba Neurosurgical Hospital.
• [Book] An application of fluorescence analysis in tumor tissue.(Eds.by 三好憲雄, S.Kaneko; pp.1-92)(Recent Progress on Clinical and Basic Research of ALA.)(*Corresponding Author) (2007)
  • Author(s): T.Ogasawara, N.Miyoshi^*, K.Sano.
  • Publisher: Published by Kashiwaba Neurosurgical Hospital.
• [Book] Chapter 4.New Approaches Detection Secondary Conformation of Prion-Section Tissue by Fourier-Transform Microscopy.(Eds.by K.Kneipp, R.Aroca, H.Kneipp, E.Wentrup-Byrne; pp.1-453).(New Approaches in Biomedical Spectroscopy)(*Corresponding Author). (2007)
  • Author(s): N.Miyoshi^*, H.Okada, M.Takada, M.Shinagawa, K.Akao.
  • Publisher: Published by American Chemical Society, Symposium Series,963.
• [Remarks] ホームページ等
  • URL: http://ala.umin.jp
• [Remarks]
  • URL: http://ala.umin.jp
• [Patent(Industrial Property Rights)] 粒子線治療を併用する光線力学治療法 (2010)
  • Inventor(s): 三好憲雄, 久米恭
  • Industrial Property Rights Holder: 福井大学若狭湾エネルギー研究センター
  • Filing Date: 2010-04-13
• [Patent(Industrial Property Rights)] ヒトや動物の細胞の増殖の抑制を容易にかつ安価に実施できる方法および薬剤提供する。 (2007)
  • Inventor(s): 三好憲雄、伊藤慎治
  • Industrial Property Rights Holder: 三好憲雄
  • Industrial Property Number: 2007-263784
  • Filing Date: 2007-09-01
• [Patent(Industrial Property Rights)] 光力学的治療法に使用するための光増感剤組成物 (2007)
  • Inventor(s): 三好憲雄、杉本和広
  • Industrial Property Rights Holder: テイカ株式会社
  • Industrial Property Number: 2007-046640
  • Filing Date: 2007-06-18
• [Patent(Industrial Property Rights)] 光線力学治療法 (2007)
  • Inventor(s): 三好憲雄、久米恭
  • Industrial Property Rights Holder: (財)若狭湾エネルギー研究センター、(国立大学法人)福井大学
  • Industrial Property Number: 2009-271588
  • Filing Date: 2007-11-30
• [Patent(Industrial Property Rights)] 粒子線治療を併用する光線力学治療法 (2007)
  • Inventor(s): 三好憲雄、久米恭
  • Industrial Property Rights Holder: (財)若狭湾エネルギー研究センター、(国立大学法人)福井大学
  • Industrial Property Number: 2010-092621
  • Filing Date: 2007-04-13