レチノイン酸応答性の新規機能性RNAの同定による肝細胞癌の診療への応用
| Project/Area Number |
20390209
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Tottori University |
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Principal Investigator |
SHIOTA Goshi Tottori University, 大学院・医学系研究科, 教授 (70263457)
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| Co-Investigator(Kenkyū-buntansha) |
KURIMASA Akihiro 鳥取大学, 大学院・医学系研究科, 准教授 (80343276)
HOSHIKAWA Yoshiko 鳥取大学, 大学院・医学系研究科, 助教 (10181489)
土谷 博之 鳥取大学, 大学院・医学系研究科, 助教 (00403402)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Keywords | レチノイン酸 / 応答遺伝子 / インシリコ解析 / 肝細胞癌診療 / 応答性RNA / 肝細胞癌 / 遺伝子治療 / マイクロRNA |
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| Research Abstract |
It is important to clarify the mechanisms by which retinoid signaling prevents the hepatocarcinogenesis in detail. In the present study, genome-wide analysis of the downstream genes controlled by RA signaling was done by means of in silico and in vitro screenings. First, the in silico approach was used to pick up rnaClusters with RA responsive element (RARE) in their upstream regions, providing 201 rnaClusters (hereafter referred to as Target RNA of Retinoic Acid, TRRA). One hundred eleven of those TRRAs were known as Refseq Genes while the others were unknown naClusters. Next,we screened 201 TRRAs with chromatin immunoprecipitation and quantitative RT-PCR. As a result, it was suggested that the expression of 27 TRRAs was regulated through ATRA-dependent recruitment of retinoic acid eceptors to their RAREs. These twenty-seven TRRAs identified in this study may be useful targets of liver cancer treatment.
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Report
(4 results)
Research Products
(17 results)
