Studies on generation of peptide vaccine against cancer stem cells of pancreatic cancer using proteomic analysis
| Project/Area Number |
20390214
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | Jikei University School of Medicine |
|---|
Principal Investigator |
HOMMA Sadamu Jikei University School of Medicine, 医学部, 准教授 (50192323)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
小井戸 薫雄 東京慈恵会医科大学, 医学部, 准教授 (70266617)
伊藤 正紀 東京慈恵会医科大学, 医学部, 助教 (80297366)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
|---|
| Keywords | 膵癌 / ペプチドワクチン / プロテオーム / 癌幹細胞 / 樹状細胞 |
|---|
| Research Abstract |
We have tried to identify cancer stem cell-specific antigenic peptide from dendritic cells (DCs) loaded with side-population (SP-population) of human pancreatic cancer cells. DCs pulsed with lysate of SP-fraction or non-SP-fraction of Panc-1 cells were treated with an adequate detergent and MHC class-I/antigenic peptide complex was isolated from these DCs using anti-HLA-A02 affinity column chromatography. The several specific peptides only from DCs loaded with SP-fraction were obtained. The isolated peptide is now being analyzed for identification of a possible candidate for peptide vaccine against cancer stem cells of human pancreatic cancer.
|
Report
(4 results)
Research Products
(55 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation]2008
Author(s)
込田英夫, 小井戸薫雄, 田尻久雄, 本間定, ほか
Organizer
第12回基盤的癌免疫研究会総会
Place of Presentation
東京
Year and Date
2008-07-02
Related Report
-
-
