a study and new treatment of lung cancer stem cells

• Project/Area Number: 20390229
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Hirosaki University
• Principal Investigator: SAIJO Yasuo Hirosaki University, 大学院・医学研究科, 教授 (10270828)
• Co-Investigator(Kenkyū-buntansha): ITH JUGOH 弘前大学, 大学院・医学研究科, 准教授 (10333717)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2009: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2008: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 肺がん / がん幹細胞 / CD133 / 肺癌 / 肺幹細胞 / 肺胞上皮細胞 / SP-C / CCSP / 肺がん幹細胞 / SCIDマウス / 肺前駆細胞

Research Abstract

We identified lung cancer stem cells using CD133 as a marker. A small fraction of all 19 cell lines examined expressed CD133. Resistant cells against gefitinib showed high % of CD133 positive cells. Lung stem cells at brochiolalveolar duct junction amplified after birth and naphthalene treatment, but no after bleomycin treatment.

Research Products

• [Journal Article] Paracrine factors from Mesenchymal Stem Cells ameliorate lung injuries in elastase induced emphysema. (2011)
  • Author(s): Katsha AM, Ohkouchi S, Xin H, Kanehira M, Sun R, Nukiwa T, Saijo Y
  • Journal Title: Mol Ther 19 Pages: 196-203
• [Journal Article] Paracrine factors from Mesenchymal Stem Cells ameliorate lung injuries in elastase induced emphysema. (2011)
  • Author(s): Katsha AM, Saijo Y, et al.
  • Journal Title: Molecular Therapy Volume: 19 Pages: 196-203
  • Peer Reviewed
• [Journal Article] For the North-East Japan Study Group : Gefitinib or Chemotherapy for Non-Small Cell Lung Cancer with Mutated EGFR. (2010)
  • Author(s): Maemondo M, Inoue A, Kobayashi K, Sugawara S, Oizumi S, Isobe H, Gemma A, Harada M, Yoshizawa H, Kinoshita K, Fujita Y, Okinaga S, Hirano H, Yoshimori K, Harada T, Miyazawa H, Tanaka T, Saijo Y, Hagiwara K, Ogura T, Ando M, Morita S, Nukiwa T
  • Journal Title: New Engl J Med 362 Pages: 2380-8
• [Journal Article] Randomized phase II trial of weekly paclitaxel combined with carboplatin versus standard paclitaxel combined with carboplatin for elderly patients with advanced non-small-cell lung cancer. (2010)
  • Author(s): Sakakibara T, Inoue A, Sugawara S, Maemondo M, Ishida T, Usui K, Abe T, Kanbe M, Watanabe H, Saijo Y, Nukiwa T
  • Journal Title: Ann Oncol. 21 Pages: 795-9
• [Journal Article] Gefitinib or Chemotherapy for Non-Small Cell Lung Cancer with Mutated EGFR. (2010)
  • Author(s): Maemondo M, Saijo Y, et al.
  • Journal Title: New England of Journal of Medicine Volume: 362 Pages: 2380-2388
  • Peer Reviewed
• [Journal Article] Randomized phase II trial of weekly paclitaxel combined with carboplatin versus standard paclitaxel combined with carboplatin for elderly patients with advanced non-small-cell lung cancer. (2010)
  • Author(s): Sakakibara T, Saijo Y, et al
  • Journal Title: Annals of Oncology Volume: 21 Pages: 795-799
  • Peer Reviewed
• [Journal Article] Randomized phase II trial of weekly paclitaxel combined with carboplatin versus standard paclitaxel combined with carboplatin for elderly patients with advanced non-small-cell lung cancer. (2010)
  • Author(s): Sakakibara T, Inoue A, Sugawara S, Maemondo M, Ishida T, Usui K, Abe T, Kanbe M, Watanabe H, Saijo Y., Nukiwa T
  • Journal Title: Annals of Oncology 21 Pages: 795-799
  • Peer Reviewed
• [Journal Article] Intratracheal delivery of CX3CL1-expressing mesenchymal stem cells to muiple lung tumors. (2009)
  • Author(s): Xin H, Sun R, Kanehira M, Takahata T, Itoh J, Mizuguchi H, Saijo Y
  • Journal Title: Mol Med 15 Pages: 321-327
• [Journal Article] First-line gefitinib for advanced non-small cell lung cancer patients having epidermal growth factor receptor mutations without indication for chemotherapy. (2009)
  • Author(s): Inoue A, Kobayashi K, Usui K, Maemondo M, Okinaga S, Mikami I, Ando M, Yamazaki K, Saijo Y, Genma A, Miyazawa H, Tanaka T, Ikebuchi K, Nukiwa T, Morita S, Hagiwara K
  • Journal Title: J Clin Oncol 27 Pages: 1394-400
• [Journal Article] Intratracheal delivery of CX3CL1-expressing mesenchymal stem cells to multiple lung tumors. (2009)
  • Author(s): Xin H, Sun R, Ahmed K, Kanehira M, Mizuguchi H, Hayakawa T, Nukiwa T, Saijo Y.
  • Journal Title: Molecular Medicine 15 Pages: 321-327
  • Peer Reviewed
• [Journal Article] First-line gefitinib for advanced non-small cell lung cancer patients having epidermal growth factor receptor mutations without indication for chemotherapy. (2009)
  • Author(s): Inoue A, Kobayashi K, Usui K, Maemondo M, Okinaga S, Mikami I, Ando M, Yamazaki K, Saijo Y, Genma A, Miyazawa H, Tanaka T, Ikebuchi K, Nukiwa T, Morita S, Hagiwara K.
  • Journal Title: Journal of Clinical Oncology 27 Pages: 1394-1400
  • Peer Reviewed
• [Journal Article] Nukiwa Randomized Phase II Trial Comparing Amrubicin With Topotecan in Patients With Previously Treated Small-Cell Lung Cancer : North Japan Lung Cancer Study Group Trial 0402. (2008)
  • Author(s): Inoue A, Sugawara S, Yamazaki K, Maemondo M, Suzuki T, Gomi K, Takanashi S, Inoue C, Inage M, Yokouchi H, Watanabe H, Tsukamoto T, Saijo Y, Ishimoto O, Hommura F
  • Journal Title: J Clin Oncol 26 Pages: 5401-6
• [Journal Article] Suppression of Surfactant Protein-A by an Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor Exacerbates Lung Inflammation. (2008)
  • Author(s): Inoue A, Xin H, Suzuki T, Kanehira M, Kuroki Y, Fukuhara T, Kikuchi T, Maemondo M, Nukiwa T, Saijo Y
  • Journal Title: Cancer Science 99 Pages: 1679-84
  • NAID: 10024010194
• [Journal Article] Adenocarcinoma with epidermal growth factor receptor gene mutations in three siblings. (2008)
  • Author(s): Sakakibara T, Saijo Y, Fukuhara T, Gomi K, Inoue K, Ishimoto O, Sugawara S, Nukiwa T
  • Journal Title: J Thorac Oncol 3 Pages: 311-3
• [Journal Article] Randomized Phase II Trial Comparing Amrubicin With Topotecan in Patients With Previously Treated Small-Cell Lung Cancer : North Japan Lung Cancer Study Group Trial 0402 (2008)
  • Author(s): Inoue A, Sugawara S, Yamazak i K, Maemondo M, Saijo Y, et.al.
  • Journal Title: J Clin Oncol 26 : 5401-6, 2008 26 Pages: 5401-6
  • Peer Reviewed
• [Journal Article] Suppression of Surfactant Protein-A by an Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor Exacerbates Lung Inflammation (2008)
  • Author(s): Inoue A, Xin H, Suzuki T, Kanehira M, Saijo Y, et.al.
  • Journal Title: Cancer Science 99 Pages: 1679-84
  • NAID: 10024010194
  • Peer Reviewed
• [Journal Article] Opioid Rotation from Oral Morphine to Oral Oxycodone in Cancer Patients with Intolerable Adverse Effects : An Open-Label Trial (2008)
  • Author(s): Narabayashi M, Saijo Y, Takenoshita S, Chida M, Shimoyama
  • Journal Title: Jpn J Clin Oncol 38 Pages: 296-304
  • Peer Reviewed
• [Presentation] マウス成長肺と傷害修復過程における肺幹細胞の動態解析 (2010)
  • Author(s): 孫若文、高畑武功、石黒敦、伊東重豪、貫和敏博、西條康夫
  • Organizer: 第50回日本呼吸器学会学術講演会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2010-04-24
• [Presentation] 日本人悪性リンパ腫患者における臨床病理学的因子に影響する遺伝子多型 (2009)
  • Author(s): 高畑武功、伊東重豪、花田直之、孫若文、鈴木一宏、鬼島宏、伊東健、福田真作、西條康夫
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-02
• [Presentation] 日本人悪性リンパ腫患者における臨床病理学的因子に影響する遺伝子多型 (2009)
  • Author(s): 高畑武功、伊東重豪、花田直之、孫若文、鈴木一宏、鬼島宏、伊東健、福田真作、西條康夫
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-10-02
• [Presentation] ヒト大腸癌細胞株におけるkeap1プロモーター領域のメチル化 (2009)
  • Author(s): 花田直之、高畑武功、伊東重豪、孫若文、鈴木一宏、石黒敦、坂田優、福田真作、西條康夫
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-01
• [Presentation] ヒト大腸癌細胞株におけるkeap 1プロモーター領域のメチル化 (2009)
  • Author(s): 花田直之、高畑武功、伊東重豪、孫若文、鈴木一宏、石黒敦、坂田優、福田真作、西條康夫
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-01
• [Presentation] マウス肺の成長過程における肺幹細胞の動態解析 (2009)
  • Author(s): 孫若文、辛紅、高畑武功、伊東重豪、鬼島宏、貫和敏博、西條康夫
  • Organizer: 日本呼吸器学会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2009-06-12
• [Presentation] 間葉系幹細胞は生体の腫瘍増殖を促進させる (2008)
  • Author(s): 鈴木一広、折口真人、兼平雅彦、孫若文、花田直之、高畑武功、伊東重豪、西條康夫
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Remarks] ホームページ等