Induction of intermediate mesoderm and kidney progenitors from embryonic stem cells
| Project/Area Number |
20390237
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Satomi 熊本大学, 発生医学研究所, 助教 (10321944)
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| Project Period (FY) |
2008 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Keywords | ES細胞 / 腎臓発生 / 幹細胞 |
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| Research Abstract |
This project aims at induction of the intermediate mesoderm and metanephric mesenchyme, which contains nephron progenitors, from embryonic stem(ES) cells. We generated a knock-in mouse strain in which these two kidney precursor populations were marked with GFP. Sorted GFP-positive cells were subjected to the colony-forming assay upon Wnt4 stimulation. We revealed that embryos at E9. 5, but not at E8. 5, contained colony-forming cells. As GFP-positive cells induced from ES cells were unable to form colonies, we assume that they may correspond to the E8. 5 intermediate mesoderm. Further investigation is needed to derive nephron progenitors from ES cells.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Kif26b, a kinesin family gene, regulates adhesion of the embryonic kidney mesenchyme2010
Author(s)
Uchiyama Y, Sakaguchi M, Terabayashi T, Inenaga T, Inoue S, Kobayashi C, Oshima N, Kiyonari H, Nakagata N, Sato Y, Sekiguchi K, Miki H, Araki E, Fujimura S, Tanaka SS, and Nishinakamura R.
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 107(20)
Pages: 9240-9245
Related Report
Peer Reviewed
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