| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Research Abstract |
This project aims at induction of the intermediate mesoderm and metanephric mesenchyme, which contains nephron progenitors, from embryonic stem(ES) cells. We generated a knock-in mouse strain in which these two kidney precursor populations were marked with GFP. Sorted GFP-positive cells were subjected to the colony-forming assay upon Wnt4 stimulation. We revealed that embryos at E9. 5, but not at E8. 5, contained colony-forming cells. As GFP-positive cells induced from ES cells were unable to form colonies, we assume that they may correspond to the E8. 5 intermediate mesoderm. Further investigation is needed to derive nephron progenitors from ES cells.
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