| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2010: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2008: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Research Abstract |
Allele-specific gene silencing by RNAi (allele-specific RNAi : ASP-RNAi) is an advanced application of RNAi techniques, by which the expression of an allele of interest can be specifically inhibited. Thus, ASP-RNAi is therapeutically useful for specifically inhibiting the expression of disease-causing alleles without suppressing the expression of corresponding wild-type alleles. To achieve ASP-RNAi, it is vital to design small interfering RNAs (siRNAs) conferring allele discrimination. We developed an easy assay system with reporter alleles. Using our assay system, we successfully designed and selected competent siRNAs conferring ASP-RNAi against mutant Prion Protein (PRNP) and Huntingtin (HTT) genes.
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