The role of vaspin in metabolic syndrome

• Project/Area Number: 20390257
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Okayama University
• Principal Investigator: WADA Jun Okayama University, 大学院・医歯薬学総合研究科, 准教授 (30294408)
• Co-Investigator(Kenkyū-buntansha): MAKINO Hirofumi 岡山大学, 大学院・医歯薬学総合研究科, 教授 (50165685)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2008: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: メタボリックシンドローム / インスリン抵抗性 / アディポカイン / バスピン / 糖尿病 / 脂質異常症 / 高血圧 / 肥満症 / メタボリック症候群 / 肥満

Research Abstract

Vaspin is a novel adipocytokine, which was identified from visceral adipose tissues in obese rats. The injection of recombinant vaspin into obese mice and experiments using vaspin transgenic and knockout mice revealed that vaspin is a compensatory factor for insulin resistance in metabolic syndrome. Vaspin circulated in the sera of human subjects and it positively correlated with body mass index and degree of insulin resistance. Serum levels of vaspin were higher in patients with type 2 diabetes compared with normal healthy subjects. The regulation of serum concentration of vaspin differed from leptin and adiponectin and vaspin may be a new therapeutic target for metabolic syndrome.

Research Products

• [Journal Article] Effects of icodextrin peritoneal dialysis solution on the peritoneal membrane in the STZ-induced diabetic rat model with partial nephrectomy. (2010)
  • Author(s): Nakao A, Nakao K, Takatori Y, Kojo S, Inoue J, Akagi S, Sugiyama H, Wada J, Makino H
  • Journal Title: Nephrol Dial Transplant 25(5) Pages: 1479-1488
  • Peer Reviewed
• [Journal Article] Comparison of insulin detemir and insulin glargine on glycemic variability in patients with type 1 and type 2 diabetes. (2010)
  • Author(s): Tone A, Iseda I, Higuchi C, Tsukamoto K, Katayama A, Matsushita Y, Hida K, Wada J, Shikata K
  • Journal Title: Exp Clin Endocrinol Diabetes 118(5) Pages: 320-324
  • Peer Reviewed
• [Journal Article] In vivo delivery of gremlin siRNA plasmid reveals therapeutic potential against diabetic nephropathy by recovering bone morphogenetic protein-7. (2010)
  • Author(s): Zhang Q, Shi Y, Wada J, Malakauskas SM, Liu M, Ren Y, Du C, Duan H, Li Y, Li Y, Zhang Y
  • Journal Title: PLoS ONE 5(7)
  • Peer Reviewed
• [Journal Article] Polymorphisms in NRXN3, TFAP2B, MSRA, LYPLAL1, FTO and MC4R and their effect on visceral fat area in the Japanese population. (2010)
  • Author(s): Hotta K, Nakamura M, Nakamura T, Matsuo T, Nakata Y, Kamohara S, Miyatake N, Kotani K, Komatsu R, Itoh N, Mineo I, Wada J, Yoneda M, Nakajima A, Funahashi T, Miyazaki S, Tokunaga K, Kawamoto M, Masuzaki H, Ueno T, Hamaguchi K, Tanaka K, Yamada K, Hanafusa T, Oikawa S, Yoshimatsu H, Nakao K, Sakata T, Matsuzawa Y, Nakamura Y, Kamatani N
  • Journal Title: J Hum Genet 55(11) Pages: 738-742
  • NAID: 10030737572
  • Peer Reviewed
• [Journal Article] Effects of icodextrin peritoneal dialysis solution on the peritoneal membrane in the STZ-induced diabetic rat model with partial nephrectomy. (2010)
  • Author(s): Nakao A, Nakao K, Takatori Y, Kojo S, Inoue J, Akagi S, Sugiyama H, Wada J, Makino H
  • Journal Title: Nephrol Dial Transplant Volume: 25(5) Pages: 1479-1488
  • Peer Reviewed
• [Journal Article] Comparison of insulin detemir and insulin glargine on glycemic variability in patients with type 1 and type 2 diabetes. (2010)
  • Author(s): Tone A, Iseda I, Higuchi C, Tsukamoto K, Katayama A, Matsushita Y, Hida K, Wada J, Shikata K
  • Journal Title: Exp Clin Endocrinol Diabetes Volume: 118(5) Pages: 320-324
  • Peer Reviewed
• [Journal Article] In vivo delivery of gremlin siRNA plasmid reveals therapeutic potential against diabetic nephropathy by recovering bone morphogenetic protein-7 (2010)
  • Author(s): Zhang Q, Shi Y, Wada J, Malakauskas SM, Liu M, Ren Y, Du C, Duan H, Li Y, Li Y, Zhang Y
  • Journal Title: PLoS ONE Volume: 5(7)
  • Peer Reviewed
• [Journal Article] Polymorphisms in NRXN3, TFAP2B, MSRA, LYPLAL1, FTO and MC4R and their effect on visceral fat area in the Japanese population. (2010)
  • Author(s): Hotta K, Nakamura M, Nakamura T, Matsuo T, Nakata Y, Kamohara S, Miyatake N, Kotani K, Komatsu R, Itoh N, Mineo I, Wada J, Yoneda M, Nakajima A, Funahashi T, Miyazaki S, Tokunaga K, Kawamoto M, Masuzaki H, Ueno T, Hamaguchi K, Tanaka K, Yamada K, Hanafusa T, Oikawa S, Yoshimatsu H, Nakao K, Sakata T, Matsuzawa Y, Nakamura Y, Kamatani N
  • Journal Title: J Hum Genet Volume: 55(11) Pages: 738-742
  • NAID: 10030737572
  • Peer Reviewed
• [Journal Article] Screening of 336 single-nucleotide polymorphisms in 85 obesity-related genes revealed McKusick-Kaufman syndrome gene variants are associated with metabolic syndrome. (2009)
  • Author(s): Hotta K, Nakamura T, Takasaki J, Takahashi H, Takahashi A, Nakata Y, Kamohara S, Kotani K, Komatsu R, Itoh N, Mineo I, Wada J, Masuzaki H, Yoneda M, Nakajima A, Funahashi T, Miyazaki S, Tokunaga K, Hamaguchi K, Tanaka K, Yamada K, Hanafusa T, Oikawa S, Yoshimatsu H, Nakao K, Sakata T, Matsuzawa Y, Kamatani N, Nakamura Y
  • Journal Title: J Hum Genet 54(4) Pages: 230-235
  • NAID: 10030730053
  • Peer Reviewed
• [Journal Article] Enhanced interaction between focal adhesion and adherens junction proteins : involvement in sphingosine 1-phosphate-induced endothelial barrier enhancement. (2009)
  • Author(s): Sun X, Shikata Y, Wang L, Ohmori K, Watanabe N, Wada J, Shikata K, Birukov KG, Makino H, Jacobson JR, Dudek SM, Garcia JG
  • Journal Title: Microvasc Res 77(3) Pages: 304-313
  • Peer Reviewed
• [Journal Article] Endothelial barrier protection by FTY720 under hyperglycemic condition : involvement of focal adhesion kinase, small GTPases, and adherens junction proteins. (2009)
  • Author(s): Sarai K, Shikata K, Shikata Y, Omori K, Watanabe N, Sasaki M, Nishishita S, Wada J, Goda N, Kataoka N, Makino H
  • Journal Title: Am J Physiol Cell Physiol 297(4)
  • Peer Reviewed
• [Journal Article] Association between obesity and polymorphisms in SEC16B, TMEM18, GNPDA2, BDNF, FAIM2 and MC4R in a Japanese population. (2009)
  • Author(s): Hotta K, Nakamura M, Nakamura T, Matsuo T, Nakata Y, Kamohara S, Miyatake N, Kotani K, Komatsu R, Itoh N, Mineo I, Wada J, Masuzaki H, Yoneda M, Nakajima A, Funahashi T, Miyazaki S, Tokunaga K, Kawamoto M, Ueno T, Hamaguchi K, Tanaka K, Yamada K, Hanafusa T, Oikawa S, Yoshimatsu H, Nakao K, Sakata T, Matsuzawa Y, Kamatani N, Nakamura Y
  • Journal Title: J Hum Genet 54(12) Pages: 727-731
  • NAID: 10030732862
  • Peer Reviewed
• [Journal Article] Screening of 336 single-nucleotide polymorphisms in 85 obesity-related genes revealed McKusick-Kaufman syndrome gene variants are associated with metabolic syndrome. (2009)
  • Author(s): Hotta K, (以下25名省略), Wada J, Nakamura Y
  • Journal Title: J Hum Genet. 54(4) Pages: 230-235
  • NAID: 10030730053
  • Peer Reviewed
• [Journal Article] Enhanced interaction between focal adhesion and adberens junction proteins : involvement in sphingosine 1-phosphate-induced endothelial barrier enbancement. (2009)
  • Author(s): Sun X, Shikata Y, Wang L, Ohmori K, Watanabe N, Wada J, Shikata K, Birukov KG, Makino H, Jacobson JR, Dudek SM, Garcia JG
  • Journal Title: Microvasc Res 77(3) Pages: 304-313
  • Peer Reviewed
• [Journal Article] Urinary excretions of lipocalin-type prostaglandin D synthase predict renal injury in type-2 diabetes : a cross-sectional and prospective multicentre study. (2009)
  • Author(s): Uehara Y, Makino H, Seiki K, Urade Y, LPGDS Clinical Research Group of Kidney
  • Journal Title: Nephrol Dial Transplant 24(2) Pages: 475-482
  • Peer Reviewed
• [Journal Article] Endothelial barrier protection by FTY720 under hyperglycemic condition : involvement of focal adhesion kinase, small GTPases, and adherens junctionproteins. (2009)
  • Author(s): Sarai K, Shikata K, Shikata Y, Omori K, Watanabe N, Sasaki M, Nishishita S, Wada J, Goda N, Kataoka N, Makino H
  • Journal Title: Am J Physiol Cell Physiol 297(4)
  • Peer Reviewed
• [Journal Article] Effects of icodextrin peritoneal dialysis solution on the peritoneal membrane in the STZ-induced diabetic rat model with partial nephrectomy. (2009)
  • Author(s): Nakao A, Nakao K, Takatori Y, Kojo S, Inoue J, Akagi S, Sugiyama H, Wada J, Makino H
  • Journal Title: Nephrol Dial Transplant (In press)
  • Peer Reviewed
• [Journal Article] Procollagen C-proteinase enhancer-1 (PCPE-1) interacts with beta(2)-microglobulin (beta2-m) and may help initiate beta2-m amyloid fibril formation in connective tissues. (2008)
  • Author(s): Morimoto H, Wada J, Font B, Mott JD, Hulmes DJ, Ookoshi T, Naiki H, Yasuhara A, Nakatsuka A, Fukuoka K, Takatori Y, Ichikawa H, Akagi S, Nakao K, Makino H
  • Journal Title: Matrix Biol 27(3) Pages: 211-219
  • Peer Reviewed
• [Journal Article] Comparison of serum uric acid levels between Japanese with and without metabolic syndrome. (2008)
  • Author(s): Numata T, Miyatake N, Wada J, Makino H
  • Journal Title: Diabetes Res Clin Pract 80(1)
  • Peer Reviewed
• [Journal Article] Amelioration of high glucose-induced renal cell mitochondrial dysfunction by Rap1b GTPase. (2008)
  • Author(s): Sun L, Xie P, Wada J, Kashihara N, Lie F-Y, Zhao Y, Kumar D, Chugh SS, Danesh FR, Kanwar YS
  • Journal Title: J Am Soc Nephrol 19(12) Pages: 2293-2301
  • Peer Reviewed
• [Journal Article] Glycated albumin levels predict long-term survival in diabetic patients undergoing hemodialysis. (2008)
  • Author(s): Fukuoka K, Nakao K, Morimoto H, Nakao A, Takatori Y, Arimoto K, Taki M, Wada J, Makino H
  • Journal Title: Nephrology (Carlton) 13(4) Pages: 278-283
  • Peer Reviewed
• [Journal Article] Variations in the FTO gene are associated with severe obesity in the Japanese. (2008)
  • Author(s): Hotta K, Nakata Y, Matsuo T, Kamohara S, Kotani K, Komatsu R, Itoh N, Mineo I, Wada J, Masuzaki H, Yondeda M, Nakajima A, Miyazaki S, Tokunaga K, Kawamoto M, Funahashi T, Hamaguchi K, Yamada K, Hanafusa T, Oikawa S, Yoshimatsu H, Nakao K, Sakata T, Matsuzawa Y, Tanaka K, Kamatani N, Nakamura Y
  • Journal Title: J Hum Genet 53(6) Pages: 546-553
  • NAID: 10021249391
  • Peer Reviewed
• [Journal Article] INSIG2 gene rs7566605 polymorphism is associated with severe obesity in Japanese. (2008)
  • Author(s): Hotta K, Nakamura M, Nakata Y, Matsuo T, Kamohara S, Kotani K, Komatsu R, Itoh N, Mineo I, Wada J, Masuzaki H, Yoneda M, Nakajima A, Miyazaki S, Tokunaga K, Kawamoto M, Funahashi T, Hamaguchi K, Yamada K, Hanafusa T, Oikawa S, Yoshimatsu H, Nakao K, Sakata T, Matsuzawa Y, Tanaka K, Kamatani N, Nakamura Y
  • Journal Title: J Hum Genet 53(9) Pages: 857-862
  • NAID: 10021929575
  • Peer Reviewed
• [Journal Article] Collectrin is involved in the development of salt-sensitive hypertension by facilitating the membrane trafficking of apical membrane proteins via interaction with SNARE complex. (2008)
  • Author(s): Yasuhara A, Wada J, Malakauskas SM, Zhang Y, Eguchi J, Nakatsuka A, Murakami K, Kanzaki M, Teshigawara S, Yamagata K, Le TH, Makino H
  • Journal Title: Circulation 118 Pages: 2146-2155
  • Peer Reviewed
• [Journal Article] Procollagen C-proteinase enhancer-1 (PCPE-1)interacts with beta(2)-microglobulin (beta2-m)and may help initiate beta2-mamyloid fibril formation in connective tissues. (2008)
  • Author(s): Morimoto H, Wada J, FontB, Mott JD, Hulmes DJ, Ookoshi T, Naiki H, Yasuhara A, Nakatsuka A, Fukuoka K, Takatori Y, Ichikawa H, Akagi S, Nakao K, Makino H
  • Journal Title: Matrix Biol 27(3) Pages: 211-219
  • Peer Reviewed
• [Journal Article] Comparison of serum uric acid levels between Japanese with and without metabolic syndrome. (2008)
  • Author(s): Numata T, Miyatake N, Wada J, Makino H
  • Journal Title: Diabetes Res Clin Prac 80(1)
  • Peer Reviewed
• [Journal Article] Vaspin : a novel serpin with insulin-sensitizing effects. (2008)
  • Author(s): Wada J
  • Journal Title: Expert Opin lnvestig Drugs 17(3) Pages: 327-333
  • Peer Reviewed
• [Journal Article] Diabetic nephropathy : mechanism of renal disease progression. (2008)
  • Author(s): Kanwar YS, Wada J. Sun L, Xie P, Wallner El, Chen s, Chugh s, Danesh FR
  • Journal Title: Exp Biol Med (Maywood) 233(1) Pages: 4-11
  • Peer Reviewed
• [Journal Article] Variations in the FTO gene are associated with severe obesity in the Japanese. (2008)
  • Author(s): Hotta K, Nakata Y, Matsuo T, Kamohara S, Kotani K, Komatsu R, ltoh N, Mineo I, Wada J, Masuzaki H, Yondeda M, Nakajima A, Miyazaki S, Tokunaga K, Kawamoto M, Funahashi T, Hamaguchi K, Yamada K, Hanafusa T, Oikawa S, Yoshimatsu H, Nakao K, Sakata T, Matsuzawa Y, Tanaka K, Kamatani N, Nakamura Y
  • Journal Title: J Hum Genet 53(6) Pages: 546-553
  • NAID: 10021249391
  • Peer Reviewed
• [Journal Article] INSIG2 gene rs7566605 polymorphism is associated with severe obesity in Japanese. (2008)
  • Author(s): Hotta, K, Nakamura, M, Nakata, Y, Matsuo, T, Kamohara, S, Kotani, K, Komatsu, R, ltoh, N, Mineo, |, Wada, J, Masuzaki, H, Yoneda, M, Nakajima, A, Miyazaki, S, Tokunaga, K, Kawamoto, M, Funahashi, T, Hamaguchi, K, Yamada, K, Hanafusa, T, Oikawa, S, Yoshimatsu, H, Nakao, K, Sakata, T, Matsuzawa, Y, Tanaka, K, Kamatani, N, Nakamura, Y
  • Journal Title: J Hum Genet 53(9) Pages: 857-862
  • NAID: 10021929575
  • Peer Reviewed
• [Journal Article] Collectrin is involved in the development of salt-sensitive hypertension by facilitating the membrane trafficking of apicalmembrane proteins via interaction with SNARE complex. (2008)
  • Author(s): Yasuhara A, Wada J, Malakauskas SM, Zhang Y, Eguchi J, Nakatsuka A, Murakami K, Kanzaki M, Teshigawara S, Yamagata K, Le TH, Makino H
  • Journal Title: Circulation 118 Pages: 2146-2155
  • Peer Reviewed
• [Presentation] コレクトリン:その機能と異常 (2010)
  • Author(s): 和田淳
  • Organizer: 第53回日本腎臓学会
  • Place of Presentation: 神戸ポートピアホテル(神戸市)
  • Year and Date: 2010-06-16
• [Presentation] Vaspin as a New Therapeutic Target for Diabetes and Obesity. (2009)
  • Author(s): Wada J
  • Organizer: BIT Life Sciences' 7^<th> Annual Congress of International Drug Discovery Sciences & Technology-2009
  • Place of Presentation: Shanghai, China
  • Year and Date: 2009-10-24
• [Presentation] Vaspin as a New Therapeutic Target for Diabetes and Obesity. (2009)
  • Author(s): Wada J, 他 共著
  • Organizer: BIT Life Sciences' 7th Annual Congress of International Drug Discovery Sciences & Technology-2009
  • Place of Presentation: 上海
  • Year and Date: 2009-10-24
• [Presentation] Vaspin, A Novel Adipokine with Insulin-Sensitizing Effects. (2008)
  • Author(s): Wada J
  • Organizer: GTCbio's 4th Metabolic Diseases World Summit
  • Place of Presentation: Boston, USA
  • Year and Date: 2008-11-06
• [Presentation] Vaspin, A Novel Adipokine with Insulin Sensitizing Effects. (2008)
  • Author(s): Wada J
  • Organizer: GTCbio's 4th Metabolic Diseases World Summit
  • Place of Presentation: Boston, US
  • Year and Date: 2008-11-06
• [Presentation] Drug discovery related to vaspin, visceral adipose tissue-derived serine protease inhibitor, a novel adipokine with insulin-sensitizing effects. (2008)
  • Author(s): Wada J
  • Organizer: EHRLICH II, 2nd World Conference on Magic Bullets
  • Place of Presentation: Nurnberg, Germany
  • Year and Date: 2008-10-04
• [Book] Vaspin : Visceral Adipose Tissue-Derived Serpin with Insulin-Sensitizing Effects(Ed Bojidor Georgiev and Sava Markovski)(In Serpins and Protein Kinase Inhibitors : Novel Functions, Structural Features and Molecular Mechanisms, Nova Science Publishers, Hauppauge NY) (2009)
  • Author(s): Wada J
• [Book] Serpins and Protein Kinase Inhibitors : Novel Functions, Structural Features and Molecular Mechanisms (2009)
  • Author(s): Wada J
  • Total Pages: 257
  • Publisher: Nova Science Publishers